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Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique 被引量:4
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作者 骆蒙 孔秀英 +3 位作者 姜涛 贾川 周荣华 贾继增 《Acta Botanica Sinica》 CSCD 2002年第5期567-572,共6页
Powdery mildew is a serious disease of wheat in China. As part of ITEC (International Triteace EST cooperation), EST (expressed sequence tags) technique was used to explore the gene expression in leaf induced by Ery... Powdery mildew is a serious disease of wheat in China. As part of ITEC (International Triteace EST cooperation), EST (expressed sequence tags) technique was used to explore the gene expression in leaf induced by Erysiphe graminis DC. A conventional cDNA library was constructed, and a total of 1 500 clones picked randomly from the library were sequenced, three hundred and eighty_seven ESTs of them were unique, which got the Accession Number in GenBank. About 49.4% ESTs showed significant similarity to functions of known sequences in GenBank. There are 196 ESTs' with functions not able to be determined, and eighty_four ESTs were demonstrated to be novel sequences. High_density dot membranes from unique clones were produced, and several disease resistance related genes were screened by differential hybridization. 展开更多
关键词 WHEAT erysiphe graminis expressed sequence tags disease resistance gene
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