Total RNA from leaves of Poapratensis cultivars under drought stress was extracted for reversing transcription to cDNA and then cDNA as template for PCR reaction by designing primer of cds of Hordeum valgare HVA1 drou...Total RNA from leaves of Poapratensis cultivars under drought stress was extracted for reversing transcription to cDNA and then cDNA as template for PCR reaction by designing primer of cds of Hordeum valgare HVA1 drought resistance gene from GenBank. The amplified products were positive recon identified by using procedures of recovery, connection, transformation and enzyme separation. The length of cloned gene sequence was 324 bp, identity reached 79.27% with Barley HVA1 gene that meaned the cloned gene sequence was the partial HVA1 gene of Poapratensis.展开更多
基金Supported by Committee of Nature Science Foundation of Heilongjiang Province (C0207)
文摘Total RNA from leaves of Poapratensis cultivars under drought stress was extracted for reversing transcription to cDNA and then cDNA as template for PCR reaction by designing primer of cds of Hordeum valgare HVA1 drought resistance gene from GenBank. The amplified products were positive recon identified by using procedures of recovery, connection, transformation and enzyme separation. The length of cloned gene sequence was 324 bp, identity reached 79.27% with Barley HVA1 gene that meaned the cloned gene sequence was the partial HVA1 gene of Poapratensis.
