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HPV感染病例与P16^(INK4a)表达的关联研究
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作者 牛爱琴 杨苗苗 +2 位作者 朱冰 王艳春 竞柏松 《黑龙江医药科学》 2024年第1期71-74,共4页
目的:研究P16^(INK4a)在宫颈炎症与宫颈鳞状上皮内病变以及癌组织中的免疫表达及其与临床病理特征和HPV-DNA分型的相关性。方法:收集商丘市第一人民医院2020年4月至2023年4月404例宫颈病变组织病理活检,包括炎症组织、宫颈鳞状上皮内病... 目的:研究P16^(INK4a)在宫颈炎症与宫颈鳞状上皮内病变以及癌组织中的免疫表达及其与临床病理特征和HPV-DNA分型的相关性。方法:收集商丘市第一人民医院2020年4月至2023年4月404例宫颈病变组织病理活检,包括炎症组织、宫颈鳞状上皮内病变以及鳞状细胞癌(SCC);统计所有病例的P16^(INK4a)免疫组化染色与HPV-DNA分型结果,并分析各病变组织中的P16^(INK4a)与HPV分型关系。结果:P16^(INK4a)的表达在各种宫颈病变组织中存在显著差异(P<0.05),且P16^(INK4a)的阳性率与表达强度随宫颈病变程度的加深而增加;宫颈组织中P16^(INK4a)的表达在低危型与高危型HPV间有显著差异(P<0.05),P16^(INK4a)的表达强度与高危型HPV有相关性(P<0.001)。结论:P16^(INK4a)免疫染色结合HPV-DNA分型能有助于鉴别高级别CIN和宫颈癌,以及区分这些病变与低级别CIN和正常宫颈组织。 展开更多
关键词 宫颈病变 P16^(ink4a) HPV分型 免疫组化
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美洲大蠊提取物CⅡ-3调控p16^(INK4a)诱导SKOV3细胞衰老 被引量:1
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作者 毕子莹 周玥 +4 位作者 张成桂 刘衡 汪小波 田露 吴渟 《医学研究与教育》 CAS 2024年第1期1-8,共8页
目的探讨美洲大蠊提取物CⅡ-3调控p16^(INK4a)蛋白诱导卵巢癌细胞SKOV3衰老的作用。方法通过CCK-8法测定不同浓度和时间美洲大蠊提取物CⅡ-3对SKOV3细胞增殖的作用;运用细胞集落形成实验测定美洲大蠊提取物CⅡ-3对SKOV3细胞增殖能力的影... 目的探讨美洲大蠊提取物CⅡ-3调控p16^(INK4a)蛋白诱导卵巢癌细胞SKOV3衰老的作用。方法通过CCK-8法测定不同浓度和时间美洲大蠊提取物CⅡ-3对SKOV3细胞增殖的作用;运用细胞集落形成实验测定美洲大蠊提取物CⅡ-3对SKOV3细胞增殖能力的影响;流式细胞术检测CⅡ-3作用后SKOV3细胞周期的变化;衰老相关-β-半乳糖苷酶(SA-β-Gal)染色检测CⅡ-3作用后SKOV3细胞衰老变化;Western Blotting检测CⅡ-3作用后SKOV3细胞衰老调控分子p16^(INK4a)蛋白表达的变化。结果美洲大蠊提取物CⅡ-3可有效抑制SKOV3细胞增殖,最佳作用浓度为80μg/mL,作用时间为48 h;CⅡ-3作用后,SKOV3细胞集落形成能力下降;阻滞于G_(0)/G_(1)期细胞比例增高,S期细胞比例减少;SA-β-Gal阳性细胞百分比增加;衰老调控分子p16^(INK4a)蛋白表达上调。结论美洲大蠊提取物CⅡ-3通过调控衰老调控分子p16^(INK4a)蛋白表达诱导SKOV3细胞衰老。 展开更多
关键词 美洲大蠊提取物CⅡ-3 SKOV3细胞 衰老 p16^(ink4a)蛋白
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Alleviation of the plastic deformation of gel ink under strong stress through an esterification of xanthan gum reinforcing its double helix structure
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作者 Xiaokun Li Mingyi Wang +5 位作者 Zilu Liu Song Yang Na Xu Wei Zhao Gan Luo Shoujun Liu 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2024年第3期49-57,共9页
As a natural organic polymer,xanthan gum(XG)can alleviate the plastic deformation of gel ink under strong stress and realize the reasonable regulation of the rheological properties of gel ink.However,as the double-hel... As a natural organic polymer,xanthan gum(XG)can alleviate the plastic deformation of gel ink under strong stress and realize the reasonable regulation of the rheological properties of gel ink.However,as the double-helix structure connected by hydrogen bonds cannot resist the mechanical environment of strong stress,XG shows poor shear resistance.In this study,a polymer gel with interpenetrating polymer network structure was prepared by esterifying XG,taking polystyrene maleic anhydride(SMA)as the modifier.In addition to retaining the excellent rheological properties of XG,the generated polymer gel also exhibited high shear resistance.The optimal addition amount of the esterification reaction modifier was determined as mXG:mSMA=5:3 according to the gel ink standard.With this amount,the viscosity of the modified xanthan gum(SXG)gel increased to 1578.8 mPa·s and 100.7 mPa·s at shear rates of 4 s1 and 383 s1,respectively,and the shear resistance increased more than 2 times compared to the unmodified one.It is because of the ester bond formed by esterification that the reaction strengthens the interaction between molecular segments,enabling the new gel to resist to strong mechanical stress.The new polymer gel studied in this paper and the proposed mechanism of action provide new insights for the development of high-end gel ink and also provide theoretical support for the study of rheological properties of non-Newtonian fluids. 展开更多
关键词 Gel ink Xanthan gum ESTERIFICATION Simulation Shear resistance GELS
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Naturally Crosslinked Biocompatible Carbonaceous Liquid Metal Aqueous Ink Printing Wearable Electronics for Multi-Sensing and Energy Harvesting
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作者 King Yan Chung Bingang Xu +3 位作者 Di Tan Qingjun Yang Zihua Li Hong Fu 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第8期148-168,共21页
Achieving flexible electronics with comfort and durability comparable to traditional textiles is one of the ultimate pursuits of smart wearables.Ink printing is desirable for e-textile development using a simple and i... Achieving flexible electronics with comfort and durability comparable to traditional textiles is one of the ultimate pursuits of smart wearables.Ink printing is desirable for e-textile development using a simple and inexpensive process.However,fabricating high-performance atop textiles with good dispersity,stability,biocompatibility,and wearability for high-resolution,large-scale manufacturing,and practical applications has remained challenging.Here,waterbased multi-walled carbon nanotubes(MWCNTs)-decorated liquid metal(LM)inks are proposed with carbonaceous gallium–indium micro-nanostructure.With the assistance of biopolymers,the sodium alginate-encapsulated LM droplets contain high carboxyl groups which non-covalently crosslink with silk sericin-mediated MWCNTs.E-textile can be prepared subsequently via printing technique and natural waterproof triboelectric coating,enabling good flexibility,hydrophilicity,breathability,wearability,biocompatibility,conductivity,stability,and excellent versatility,without any artificial chemicals.The obtained e-textile can be used in various applications with designable patterns and circuits.Multi-sensing applications of recognizing complex human motions,breathing,phonation,and pressure distribution are demonstrated with repeatable and reliable signals.Self-powered and energy-harvesting capabilities are also presented by driving electronic devices and lighting LEDs.As proof of concept,this work provides new opportunities in a scalable and sustainable way to develop novel wearable electronics and smart clothing for future commercial applications. 展开更多
关键词 BIOCOMPATIBLE Conductive ink BIOPOLYMER E-textile Carbonaceous liquid metal
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细胞学、组织P16~(INK4A)和HPV感染结果与年轻妇女CIN1进展情况的相关性研究
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作者 江慧 贾鑫华 +4 位作者 胡尚英 潘秦镜 张询 赵方辉 乔友林 《中国妇幼卫生杂志》 2024年第3期56-62,共7页
目的 探索在不同细胞学筛查结果、免疫组化染色法体现抑癌基因P16^(INK4A)表达情况和人乳头瘤病毒(human papilloma virus,HPV)感染状态下,轻度宫颈上皮内瘤样病变(cervical intraepithelial neoplasia grade Ⅰ,CIN1)人群的进展状况。... 目的 探索在不同细胞学筛查结果、免疫组化染色法体现抑癌基因P16^(INK4A)表达情况和人乳头瘤病毒(human papilloma virus,HPV)感染状态下,轻度宫颈上皮内瘤样病变(cervical intraepithelial neoplasia grade Ⅰ,CIN1)人群的进展状况。方法 回顾性分析2008—2019年149例初次宫颈活检病理为CIN1的年轻妇女的长期随访资料,以中重度宫颈上皮内瘤样病变(CIN2+)为研究终点,使用Kaplan-Meier生存分析计算CIN2+的累积发生率和平均生存时间,使用Log Rank检验比较不同检测结果组间的生存时间差异性,使用Cox回归计算细胞学筛查结果、组织P16^(INK4A)表达情况和HPV感染状态对进展为CIN2+的风险比。结果 149例CIN1人群的中位随访时间为68个月,随访结束时共有16例进展为CIN2+,CIN2+的累积发生率为10.74%。细胞学不除外高级别鳞状上皮内病变/高度鳞状上皮内病变/非典型性腺细胞的妇女进展风险是细胞学阴性/意义不明确的非典型鳞状细胞/低度鳞状上皮内病变妇女的3.04倍(95%CI:1.06~8.76,P=0.04)。组织P16^(INK4A)弥漫性染色阳性妇女CIN2+的累积发生率为14.00%,进展风险是P16^(INK4A)弥漫性染色阴性者的1.70倍(95%CI:0.63~4.58,P=0.29)。HPV-16/18感染、HPV-31/33/45/52/58感染和其他HPV高危型别感染妇女CIN2+的累积发生率分别为34.78%、9.84%和2.63%,进展风险分别是高危HPV阴性者的12.70倍(95%CI:1.58~102.16,P=0.02)、2.96倍(95%CI:0.36~24.60,P=0.32)和0.82倍(95%CI:0.05~13.05,P=0.89)。联合分析HPV感染状态和组织P16^(INK4A)染色结果时发现,HPV-16/18感染且P16^(INK4A)弥漫性阳性人群进展为CIN2+的风险是高危HPV感染和P16^(INK4A)弥漫性染色同时阴性人群的14.43倍(95%CI:1.60~130.49,P=0.02)。结论 细胞学筛查结果和HPV-16/18检测对CIN1的进展具有重要指示作用,而组织P16^(INK4A)染色作为CIN1进展参考指标的可行性还需进一步研究。 展开更多
关键词 轻度宫颈上皮内瘤样病变 细胞学 P16~(ink4A)免疫组化染色 高危型人乳头瘤病毒
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P16^(INK4a)与人乳头瘤病毒感染及宫颈上皮内瘤变进展的相关性
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作者 陈霞慧 马向薇 +1 位作者 郝冬梅 于月新 《中国医科大学学报》 CAS 北大核心 2024年第9期777-781,共5页
目的探讨P16^(INK4a)与人乳头瘤病毒(HPV)感染及宫颈上皮内瘤变(CIN)的相关性。方法选取2020年2月至2023年5月在我院就诊的CIN患者197例(CIN组),其中CIN1患者62例,CIN2患者89例,CIN3患者46例;同时选取接受子宫全切除术且术后病理证实宫... 目的探讨P16^(INK4a)与人乳头瘤病毒(HPV)感染及宫颈上皮内瘤变(CIN)的相关性。方法选取2020年2月至2023年5月在我院就诊的CIN患者197例(CIN组),其中CIN1患者62例,CIN2患者89例,CIN3患者46例;同时选取接受子宫全切除术且术后病理证实宫颈组织正常患者50例作为对照组;检测2组P16^(INK4a)表达及HPV感染情况。结果对照组P16^(INK4a)阳性表达率和HPV感染率(10.00%和0.00%)显著低于CIN组(72.08%和45.69%,P<0.05);CIN2和CIN3患者P16^(INK4a)阳性表达率(80.90%和91.30%)显著高于CIN1患者(45.16%,P<0.05)。CIN3患者HPV感染率(63.04%)高于CIN1、CIN2患者(33.87%和44.94%,P<0.05)。有HPV感染的CIN患者P16^(INK4a)阳性表达率(93.33%)显著高于无HPV感染的CIN患者(54.21%,P<0.05);HPV16/18感染患者P16^(INK4a)阳性表达率(100.00%)显著高于HPV31/33/53感染患者(85.00%,P<0.05);不同年龄、体重指数CIN患者P16^(INK4a)阳性表达率比较,差异无统计学意义(P>0.05)。结论CIN患者P16^(INK4a)表达率明显升高,且与病变程度相关,P16^(INK4a)表达与HPV感染有关。 展开更多
关键词 P16^(ink4a) 人乳头瘤病毒 宫颈上皮内瘤变 相关性
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尖锐湿疣患者组织中P16INK4A、ZnT1的表达及其与HPV感染和预后的关系
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作者 王泽淑 王万军 匡乃峰 《检验医学与临床》 CAS 2024年第13期1921-1926,共6页
目的探讨尖锐湿疣(CA)患者组织中P16INK4A蛋白、锌离子转运体1(ZnT1)mRNA表达水平及其与人乳头瘤病毒(HPV)感染的关系。方法选取2019年5月至2022年5月在该院就诊的82例CA患者作为CA组,另选取同期在该院进行包皮环切术或外阴整形术的64... 目的探讨尖锐湿疣(CA)患者组织中P16INK4A蛋白、锌离子转运体1(ZnT1)mRNA表达水平及其与人乳头瘤病毒(HPV)感染的关系。方法选取2019年5月至2022年5月在该院就诊的82例CA患者作为CA组,另选取同期在该院进行包皮环切术或外阴整形术的64名皮肤健康者作为对照组。收集CA组疣体组织及对照组的正常包皮或外阴组织,采用聚合酶链反应(PCR)-反向点杂交技术进行HPV分型检测,比较CA组与对照组,以及不同HPV分型CA患者P16INK4A与ZnT1 mRNA表达水平。所有CA患者均随访治疗8个月,复发的患者纳入复发组,未复发的患者纳入未复发组,比较两组P16INK4A与ZnT1 mRNA表达水平。绘制受试者工作特征(ROC)曲线分析P16INK4A、ZnT1单独及2项指标联合检测对CA预后复发的预测价值。结果82例CA患者中,27例为低危型感染(32.93%),25例为高危型感染(30.49%),30例为混合型感染(36.59%),感染HPV亚型包括6、11、16、18、31和51。CA组P16INK4A、ZnT1表达水平明显高于对照组,差异均有统计学意义(P<0.05)。P16INK4A高表达组45例、低表达组37例;ZnT1高表达组43例、低表达组39例。P16INK4A mRNA低表达组与高表达组年龄、性别、疣体数量、疣体位置比较,差异均无统计学意义(P>0.05),疣体直径、病程、HPV分型比较,差异均有统计学意义(P<0.05)。ZnT1 mRNA低表达组与高表达组年龄、性别、疣体数量、疣体位置比较,差异均无统计学意义(P>0.05),疣体直径、病程、HPV分型比较,差异均有统计学意义(P<0.05)。混合型、高危型P16INK4A、ZnT1 mRNA表达水平明显高于低危型,差异均有统计学意义(P<0.05);高危型P16INK4A、ZnT1 mRNA表达水平明显高于混合型,差异均有统计学意义(P<0.05)。CA患者随访8个月后,有29例复发(复发组),53例未复发(未复发组)。复发组P16INK4A、ZnT1 mRNA表达水平明显高于未复发组,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,P16INK4A、ZnT1 mRNA单独及2项指标联合检测预测CA预后复发的曲线下面积(AUC)分别为0.840、0.880、0.963,P16INK4A与ZnT1 mRNA联合检测预测CA预后复发的AUC大于2项指标单独检测(Z_(二者联合-P16INK4A)=2.097,P=0.036;Z_(二者联合-ZnT1)=2.074,P=0.038)。结论CA患者疣体组织中P16INK4A、ZnT1 mRNA表达水平明显升高,其表达水平与HPV感染密切相关。 展开更多
关键词 尖锐湿疣 P16ink4A 锌离子转运体1 人乳头瘤病毒 相关性 预后
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p16^(INK4a)在细胞衰老调控中的作用
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作者 苏浩康 夏玉熙 +1 位作者 郭凤凤 祝田田 《医学综述》 CAS 2024年第22期2710-2717,共8页
细胞衰老的原因通常包括外在和内在两个方面。外源性因素主要是细胞外基质的改变、炎症反应和衰老相关分泌表型的表达。内源性因素通常源于调节细胞周期基因的影响,如肿瘤抑制基因。p16^(INK4a)是一种肿瘤抑制因子和细胞周期调节因子,... 细胞衰老的原因通常包括外在和内在两个方面。外源性因素主要是细胞外基质的改变、炎症反应和衰老相关分泌表型的表达。内源性因素通常源于调节细胞周期基因的影响,如肿瘤抑制基因。p16^(INK4a)是一种肿瘤抑制因子和细胞周期调节因子,与细胞衰老密切相关。在衰老细胞和与年龄相关的病理过程中,p16^(INK4a)表达显著增加,p16^(INK4a)被认为是检测细胞衰老的标志物。p16^(INK4a)高表达是防止受损细胞继续分裂的保护机制,与细胞周期停滞相关,有助于防止癌症发展。因此,p16^(INK4a)的表达调控在衰老细胞的积累、细胞周期的控制和肿瘤抑制中发挥重要作用。 展开更多
关键词 细胞衰老 p16^(ink4a) 视网膜母细胞瘤蛋白 P53
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p16INK4a联合人乳头瘤病毒及液基细胞学检查在宫颈上皮内瘤变临床诊断中的应用
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作者 肖雪 王宇琦 +5 位作者 景尉 钟绍涛 赵翠柳 刘丹 谭荣韶 赖晓岚 《现代医院》 2024年第1期140-143,共4页
目的分析p16INK4a蛋白、人乳头瘤病毒及液基细胞学检查(LCT)在宫颈癌前病变筛查中的检测效率,为宫颈癌的预防和治疗提供依据。方法分析2019年1月—2020年6月广州市妇女儿童医疗中心的139例住院患者的宫颈上皮细胞p16INK4a染色、人乳头... 目的分析p16INK4a蛋白、人乳头瘤病毒及液基细胞学检查(LCT)在宫颈癌前病变筛查中的检测效率,为宫颈癌的预防和治疗提供依据。方法分析2019年1月—2020年6月广州市妇女儿童医疗中心的139例住院患者的宫颈上皮细胞p16INK4a染色、人乳头瘤病毒检测及宫颈细胞学检测结果。这些病例包括111例宫颈上皮内瘤变(CIN)患者和28例宫颈炎性疾病患者。比较三种方法单独及联合应用筛查CIN病变的效能。结果在对CIN患者的检测中,p16INK4a、生物微流控芯片和宫颈细胞学检测CIN及以上病变的灵敏度分别为91.89%、94.59%和78.20%,特异度分别为57.14%、17.86%和46.43%,AUC分别为0.75、0.56和0.66。而“p16INK4a+LCT”、“p16INK4a+hrHPV”、“LCT+hrHPV”以及三种方法联合检测的灵敏度分别为96.40%、97.30%、94.59%和99.10%,特异度分别为85.71%、92.86%、89.29%和92.86%,AUC分别为0.91、0.95、0.92和0.96。结论p16INK4a和hrHPV联合检测有助于提高诊断的准确性和早期发现率,从而使得能更早进行干预或治疗。通过这种联合应用,可以更准确地鉴别出低级别和高级别的宫颈上皮内瘤变,为个性化的病患管理提供更多信息。 展开更多
关键词 人乳头瘤病毒 生物微流控芯片 P16ink4A 液基细胞学 宫颈上皮内瘤变
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棕榈酸酯通过调控p16INK4a/Rb信号通路诱导AC16人心肌细胞发生衰老表型改变
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作者 李雯曦 林添海 +2 位作者 彭程 黄水金 刘丰 《岭南心血管病杂志》 CAS 2023年第4期433-442,共10页
目的探讨棕榈酸酯(palmitic acid,PA)在AC16人心肌细胞衰老表型改变中的作用及机制。方法AC16细胞分为(1)对照组PA浓度(0.08、0.19、0.3、0.8 mmol/L)组;(2)对照组,时间(24、36、48、72 h)刺激组。细胞计数试剂盒-8法测细胞总体活性,流... 目的探讨棕榈酸酯(palmitic acid,PA)在AC16人心肌细胞衰老表型改变中的作用及机制。方法AC16细胞分为(1)对照组PA浓度(0.08、0.19、0.3、0.8 mmol/L)组;(2)对照组,时间(24、36、48、72 h)刺激组。细胞计数试剂盒-8法测细胞总体活性,流式细胞术测细胞周期;活性氧(reactive oxygen species,ROS)试剂盒测细胞内ROS水平;β-半乳糖苷酶(SA-β-Gal)染色测染色阳性细胞率;酶联免疫吸附测定法(enzyme-linked immuno⁃sorbent assay,ELISA)测衰老相关分泌表型(senescence-associated secretory phenotype,SASP)相关因子白细胞介素(interleukin,IL)-1A、IL-6、IL-8和干扰素(interferon,IFN)-γ浓度;实时定量聚合酶链反应(quantitative real time polymerase chain reaction,qRT-PCR)及Western blot测SASP及p16^(INK4a)/视网膜母细胞瘤抑制蛋白(retinoblasto⁃ma protein,Rb)通路表达。为观察p16^(INK4a)/Rb通路对心肌衰老表型影响,细胞分为4组:对照组、PA组、si-con组和si-p16^(INK4a)组,qRT-PCR测p16^(INK4a) mRNA表达;Western blot测p16^(INK4a)、p-Rb、Rb及IL-1A、IFN-γ蛋白表达;β-半乳糖苷酶染色测阳性细胞率。结果随PA浓度及刺激时间增加,细胞活性不断下降,差异有统计学意义(P<0.05)。与对照组相比,0.19 mmol/L PA组在G1期比例显著升高,S期显著降低,差异有统计学意义(P<0.05)。0.08~0.3 mmol/L PA组ROS水平高于对照组,PA组中β-半乳糖苷酶染色阳性率均高于对照组,差异有统计学意义(P<0.05)。0.19 mmol/L PA组IL-1A、IL-6、IL-8、IFN-γ浓度显著高于对照组,差异有统计学意义(P<0.05)。0.19 mmol/LPA组IL-1A、IL-6、IL-8、IFN-γ及p16^(INK4a)、Rb mRNA及蛋白表达明显高于对照组,而p-Rb蛋白表达下降,差异有统计学意义(P<0.05)。si-p16^(INK4a)组IL-1A、IFN-γ蛋白表达及SA-β-Gal阳性率低于si-con组,差异有统计学意义(P<0.05)。结论0.19 mmol/L PA可能通过调控p16^(INK4a)/Rb通路诱导人心肌细胞衰老表型改变。 展开更多
关键词 棕榈酸酯 衰老相关分泌表型 p16ink4a/Rb 心肌衰老
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Direct ink writing to fabricate porous acetabular cups from titanium alloy 被引量:1
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作者 Naima Valentin Weijian Hua +3 位作者 Ashish K.Kasar Lily Raymond Pradeep L.Menezes Yifei Jin 《Bio-Design and Manufacturing》 SCIE EI CAS CSCD 2023年第2期121-135,共15页
Acetabular cups,which are among themost important implants in total hip arthroplasty,are usually made from titanium alloys with high porosity and adequate mechanical properties.The current three-dimensional(3D)printin... Acetabular cups,which are among themost important implants in total hip arthroplasty,are usually made from titanium alloys with high porosity and adequate mechanical properties.The current three-dimensional(3D)printing approaches to fabricate customized acetabular cups have some inherent disadvantages such as high cost and energy consumption,residual thermal stress,and relatively low efficiency.Thus,in this work,a direct ink writing method was developed to print a cup structure at room temperature,followed by multi-step heat treatment to form microscale porous structure within the acetabular cup.Our method is facilitated by the development of a self-supporting titanium-6 aluminum-4 vanadium(Ti64)ink that is composed of Ti64 particles,bentonite yield-stress additive,ultraviolet curable polymer,and photo-initiator.The effects of Ti64 and bentonite concentrations on the rheological properties and printability of inks were systematically investigated.Moreover,the printing conditions,geometrical limitations,and maximum curing depth were explored.Finally,some complex 3D structures,including lattices with different gap distances,honeycomb with a well-defined shape,and an acetabular cup with uniformly distributed micropores,were successfully printed/fabricated to validate the effectiveness of the proposed method. 展开更多
关键词 Acetabular cup Direct ink writing Titanium alloy BENTONITE Heat treatment
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The SMAD2/miR-4256/HDAC5/p16^(INK4a) signaling axis contributes to gastric cancer progression 被引量:1
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作者 MIN WANG HAILIANG ZHAO +14 位作者 WEIWEI CHEN CAIQUN BIE JINYING YANG WENRUI CAI CHUTIAN WU YANFANG CHEN SHUFEN FENG YING SHI YUTING LI HUIJUN TANG LIXIAN ZHONG LILIANGZI GUO SISI CHEN LINJING LONG SHAOHUI TANG 《Oncology Research》 SCIE 2023年第4期515-541,共27页
The dysregulation of exosomal microRNAs(miRNAs)plays a crucial role in the development and progression of cancer.This study investigated the role of a newly identified serum exosomal miRNA miR-4256 in gastric cancer(G... The dysregulation of exosomal microRNAs(miRNAs)plays a crucial role in the development and progression of cancer.This study investigated the role of a newly identified serum exosomal miRNA miR-4256 in gastric cancer(GC)and the underlying mechanisms.The differentially expressed miRNAs were firstly identified in serum exosomes of GC patients and healthy individuals using next-generation sequencing and bioinformatics.Next,the expression of serum exosomal miR-4256 was analyzed in GC cells and GC tissues,and the role of miR-4256 in GC was investigated by in vitro and in vivo experiments.Then,the effect of miR-4256 on its downstream target genes HDAC5/p16^(INK4a) was studied in GC cells,and the underlying mechanisms were evaluated using dual luciferase reporter assay and Chromatin Immunoprecipitation(ChIP).Additionally,the role of the miR-4256/HDAC5/p16^(INK4a) axis in GC was studied using in vitro and in vivo experiments.Finally,the upstream regulators SMAD2/p300 that regulate miR-4256 expression and their role in GC were explored using in vitro experiments.miR-4256 was the most significantly upregulated miRNA and was overexpressed in GC cell lines and GC tissues;in vitro and in vivo results showed that miR-4256 promoted GC growth and progression.Mechanistically,miR-4256 enhanced HDAC5 expression by targeting the promoter of the HDAC5 gene in GC cells,and then restrained the expression of p16^(INK4a) through the epigenetic modulation of HDAC5 at the p16INK4a promoter.Furthermore,miR-4256 overexpression was positively regulated by the SMAD2/p300 complex in GC cells.Our data indicate that miR-4256 functions as an oncogene in GC via the SMAD2/miR-4256/HDAC5/p16^(INK4a) axis,which participates in GC progression and provides novel therapeutic and prognostic biomarkers for GC. 展开更多
关键词 Gastric cancer miR-4256 SMAD2 HDAC5 p16^(ink4a)
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MXene ink hosting zinc anode for high performance aqueous zinc metal batteries
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作者 Jae Min Park Milan Jana +9 位作者 Sang Ha Baek Taehun Kang Peixun Xiong Jeong Hee Park Jun Soo Kim Ali Shayesteh Zeraati Mikhail Shekhirev Paul V.Braun Yury Gogotsi Ho Seok Park 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2023年第1期187-194,I0005,共9页
Despite the safety,low cost,and high theoretical capacity(820 mA h g^(-1))of Zn metal anodes,the practical application of aqueous Zn metal batteries remains a critical challenge due to the Zn dendrite growth,corrosion... Despite the safety,low cost,and high theoretical capacity(820 mA h g^(-1))of Zn metal anodes,the practical application of aqueous Zn metal batteries remains a critical challenge due to the Zn dendrite growth,corrosion,and hydrogen evolution reaction.Herein,we demonstrate the MXene ink hosting Zn metal anodes(MX@Zn)for high-performance and patternable Zn metal full batteries.The as-designed MX@Zn electrode is more facile and reversible than bare Zn and CC@Zn,as verified by better cyclic stability and lower overpotentials of symmetric cells with the plating capacity of 0.05 mA h cm^(-2)at 0.1 m A cm^(-2)and of 1 m A h cm^(-2)at 1 m A cm^(-2).The MX@Zn|MnO_(2)full cells deliver a high specific capacity of 281.9 m A h g^(-1),91.5%of the theoretical capacity,achieving 50%capacity retention from 60 mA g^(-1)to 300 mA g^(-1)and 79.7%of initial capacity after 200 cycles.Moreover,the patterned devices based on the MX@Zn electrode achieve high energy and power densities of 348.57 Wh kg^(-1)and 1556 W kg^(-1),respectively,along with a capacity retention of 64%and Coulombic efficiency of 99%over 500 cycles.The high performance of MX@Zn is attributed to the high electrical conductivity and hydrophilicity of MXene and rapid ion diffusion through the 3D interconnected porous channels. 展开更多
关键词 MXene ink Zinc anode Metal batteries PATTERNING ELECTRODEPOSITION
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A systematic printability study of direct ink writing towards high-resolution rapid manufacturing
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作者 Qingyang Zheng Bin Xie +1 位作者 Zhoulong Xu Hao Wu 《International Journal of Extreme Manufacturing》 SCIE EI CAS CSCD 2023年第3期500-517,共18页
Direct ink writing(DIW)holds enormous potential in fabricating multiscale and multi-functional architectures by virtue of its wide range of printable materials,simple operation,and ease of rapid prototyping.Although i... Direct ink writing(DIW)holds enormous potential in fabricating multiscale and multi-functional architectures by virtue of its wide range of printable materials,simple operation,and ease of rapid prototyping.Although it is well known that ink rheology and processing parameters have a direct impact on the resolution and shape of the printed objects,the underlying mechanisms of these key factors on the printability and quality of DIW technique remain poorly understood.To tackle this issue,we systematically analyzed the printability and quality through extrusion mechanism modeling and experimental validating.Hybrid non-Newtonian fluid inks were first prepared,and their rheological properties were measured.Then,finite element analysis of the whole DIW process was conducted to reveal the flow dynamics of these inks.The obtained optimal process parameters(ink rheology,applied pressure,printing speed,etc)were also validated by experiments where high-resolution(<100μm)patterns were fabricated rapidly(>70 mm s^(-1)).Finally,as a process research demonstration,we printed a series of microstructures and circuit systems with hybrid inks and silver inks,showing the suitability of the printable process parameters.This study provides a strong quantitative illustration of the use of DIW for the high-speed preparation of high-resolution,high-precision samples. 展开更多
关键词 direct ink writing extrusion mechanism modelling computational fluid dynamic(CFD) printability process parameters high-resolution printing
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p16INK4a蛋白在蒙古族患者宫颈病变组织中的表达及其意义 被引量:4
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作者 强欣 霍文艳 +3 位作者 杨灿 范亚楠 石彩云 白海花 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2017年第2期353-355,I0004,共4页
目的:检测p16INK4a蛋白在蒙古族患者宫颈病变组织中的表达,探讨其与蒙古族患者宫颈癌发生发展的关系。方法:选取100例蒙古族患者宫颈癌、宫颈上皮内瘤变(CIN)、慢性宫颈炎和子宫平滑肌瘤病变组织石蜡切片,分为宫颈癌组25例、CIN组35例... 目的:检测p16INK4a蛋白在蒙古族患者宫颈病变组织中的表达,探讨其与蒙古族患者宫颈癌发生发展的关系。方法:选取100例蒙古族患者宫颈癌、宫颈上皮内瘤变(CIN)、慢性宫颈炎和子宫平滑肌瘤病变组织石蜡切片,分为宫颈癌组25例、CIN组35例、慢性宫颈炎组20例和子宫平滑肌瘤组20例,利用免疫组织化学SP法检测各组石蜡切片组织中p16INK4a蛋白表达。结果:蒙古族患者宫颈癌、CIN、慢性宫颈炎、子宫平滑肌瘤组织中p16INK4a蛋白阳性表达率分别为100.0%、74.3%、25.0%和10.0%。多个样本比较的K-W H秩和检验,宫颈癌组p16INK4a蛋白阳性表达率高于CIN组、慢性宫颈炎组和子宫平滑肌瘤组(P<0.05)。结论:p16INK4a蛋白可以作为筛选蒙古族早期宫颈癌患者的一种检测指标。 展开更多
关键词 宫颈肿瘤 ink4a基因 P16ink4A蛋白 宫颈上皮内瘤变 慢性宫颈炎 子宫平滑肌瘤
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Analysis of the Application of Ink Art in Graphic Design Imagery
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作者 Ye Wang 《Journal of Contemporary Educational Research》 2023年第1期40-45,共6页
The graphic design industry has been developing rapidly in recent years.People have begun to focus on steering the development of graphic design in the direction of localization,integrating more traditional Chinese el... The graphic design industry has been developing rapidly in recent years.People have begun to focus on steering the development of graphic design in the direction of localization,integrating more traditional Chinese elements,raising the level of acceptance toward graphic design content,and disseminating traditional culture on this basis.Ink art plays an important role in the historical and cultural development process.It uses simple color contrast to construct different situations and possesses unique artistic charm and cultural heritage.Incorporating ink elements into graphic design may enhance the graphic design style and provide inspiration.This article focuses on the reasons,advantages,and strategies of using ink art in graphic design imagery,hoping to provide references for graphic design activities. 展开更多
关键词 ink art Graphic design IMAGERY Application analysis
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河南食管癌高发区居民食管癌组织p15INK4b和p16INK4a基因变化的研究 被引量:5
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作者 邹建湘 王立东 白永敏 《世界华人消化杂志》 CAS 1999年第3期272-272,共1页
基因丢失导致p16INK4a和(或)p15INK4b对CDK激酶的抑制作用丧失,从而导致细胞增生调节失控[1-3],目前在多种肿瘤中已发现存在p15INK4b和p16INK4a的失活现象[4-6].作者近年对河南食管... 基因丢失导致p16INK4a和(或)p15INK4b对CDK激酶的抑制作用丧失,从而导致细胞增生调节失控[1-3],目前在多种肿瘤中已发现存在p15INK4b和p16INK4a的失活现象[4-6].作者近年对河南食管癌高发区林州市居民的研究证实,肿瘤... 展开更多
关键词 河南 食管癌 p15ink4b基因 p16ink4a基因
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INK4a/ARF基因与乳腺癌的关系
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作者 张智 陈小东 《广东医学院学报》 2009年第6期675-677,共3页
INK4a/ARF是直接调控细胞周期并抑制细胞增殖的抑癌基因,本文就INK4a/ARF基因以及p53与乳腺癌的关系作一综述。
关键词 ink4A/ARF P53 P16ink4A P14ARF 乳腺癌 综述文献
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Analysis of ink flow channel between two rotating ink rollers
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作者 初红艳 Sun Ting 《High Technology Letters》 EI CAS 2015年第3期253-260,共8页
In order to research how the size of the ink flow channel is affected by the interaction of adjacent ink areas,according to the method of fluid-solid interaction,this paper analyzes the size of the ink flow channel of... In order to research how the size of the ink flow channel is affected by the interaction of adjacent ink areas,according to the method of fluid-solid interaction,this paper analyzes the size of the ink flow channel of two ink rollers' rotation.Firstly,this paper simulates the situation of only one ink area.Secondly,this paper simulates the situation of five adjacent ink areas.Then,through comparing the simulation results of two above situations,it' s obvious that the interaction of adjacent ink areas has big effects on the ink pressure and the size of ink flow channel.At last,this paper gets the main factor that affects the size of ink flow channel in the different situations. 展开更多
关键词 offset printing machine ink roller ink flow channel adjacent ink areas numeri-cal simulation
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P16^INK4A及Ki-67在宫颈腺癌组织中的表达及意义 被引量:14
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作者 刘爽 李亚里 +2 位作者 刘爱军 姜淑芳 张艾芃 《解放军医学杂志》 CAS CSCD 北大核心 2012年第3期225-228,共4页
目的探讨P16INK4A及Ki-67蛋白在宫颈腺癌组织中的表达及其临床意义。方法选取61例宫颈腺癌(宫颈腺癌组)及34例正常宫颈组织(对照组)的石蜡标本,采用SP免疫组化法测定P16INK4A及Ki-67蛋白的表达,并对61例浸润性宫颈腺癌的临床及病理资料... 目的探讨P16INK4A及Ki-67蛋白在宫颈腺癌组织中的表达及其临床意义。方法选取61例宫颈腺癌(宫颈腺癌组)及34例正常宫颈组织(对照组)的石蜡标本,采用SP免疫组化法测定P16INK4A及Ki-67蛋白的表达,并对61例浸润性宫颈腺癌的临床及病理资料进行回顾性分析。结果对照组正常宫颈腺上皮P16INK4A及Ki-67表达阳性率分别为23.53%、20.59%,而宫颈腺癌组分别为80.33%、81.97%,两组比较差异有统计学意义(P<0.05)。P16INK4A的表达与宫颈腺癌的临床分期、宫颈间质浸润深度、肿瘤直径有关(P<0.05),与宫颈腺癌的病理分型、肿瘤恶性程度及淋巴结转移无关(P>0.05)。Ki-67的表达与宫颈腺癌的病理分化程度、肿瘤直径及淋巴结转移有关(P<0.05)。子宫颈腺癌组织中P16INK4A与Ki-67的表达相关(P<0.01)。结论联合检测P16INK4A及Ki-67蛋白表达可作为宫颈腺癌的辅助诊断方法,有较好的临床应用价值。 展开更多
关键词 P16ink4A蛋白 KI-67蛋白 宫颈肿瘤
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