In Vitro Antioxidative Activities of Flavonoid from Oxytropis glabra DC

Anti-lipid peroxidation activity, reducing power and chelating power of flavonoid extracts from Oxytropis glabra DC, as well as their elimination effect on DPPH, superoxide anion and hydroxyl radicals were determined in the paper, so as to evaluate in vitro antioxidative activities of flavonoid extracted from O. glabra. The results showed that flavonoid from O. glabra had fairly strong anti-lipid peroxidation. The antioxidation effects on rapeseed oil successively were vitamin C 〉 sample 〉 vitamin E 〉 BHT 〉 CK; the effects on cottonseed oil successively were sample 〉 vitamin C 〉 BHT 〉 vitamin E 〉 CK; the effects on sunflower oil successively were sample 〉 vitamin C 〉 vitamin E 〉 BHT 〉 CK; the effects on lard successively were BHT 〉 vitamin C 〉 sample 〉 vitamin E 〉 CK. The samples had stronger clearance rate against DPPH, superoxide anion and hydroxyl radicals than control; the reducing power of sample was weaker than Vitamin C and stronger than Vitamin E ; chelating power of sample was weaker than EDTA. Flavonoid from O. glabra had the value for further development and utilization as natural antioxidant.

Oxidative effects of glyphosate on the lipophobic intracellular environment in the microalgae Chlorella vulgaris

The studied hypothesis is that the herbicide glyphosate(GLY)can affect the oxidative balance in the hydrophobic intracellular medium in non-target Chlorella vulgaris cells.Analytical GLY and RoundUp(RUP)supplementation,affected the growth profile.A significant 42%decrease in the cellular biomass in stationary(St)phase was observed in cultures supplemented with either 5μM of GLY or RUP,as compared to control cultures.The treatment with 0.3μM of GLY generated non-significant effects on the oxidation rate of 2’,7’dichlorofluorescein diacetate(DCFH-DA),neither in exponential(Exp)nor in St phase of development,as compared to control cultures.However,the treatment with either 5μM GLY or 0.3 and 5μM RUP lead to a significant decrease in the DCFH-DA oxidation rate,as compared to control cultures.The lipid radical(LR●)generation rate,detected by Paramagnetic Resonance Spectroscopy(EPR),was significantly increased in the presence of RUP,in Lag and Exp phase of growth.The non-enzymatic antioxidants,α-Tocopherol(α-T)andβ-Carotene(β-C),are aimed to protect membranes against the damage produced by the radical reactions.The content ofβ-C was not significantly affected,as compared to control cultures,by any of the treatments,in both growth phases of cellular development.The content ofα-T was significantly decreased by the supplementation with either 0.3 or 5μM of RUP or 5μM GLY.The LR●/α-T ratio,used as indicator of the oxidative balance in the hydrophobic cellular media,was significantly different between samples obtained from control and RUP-exposed microalgae in both,Exp and St phase of development,with either 0.3 or 5μM RUP.The data presented here showed evidence that suggested that oxidative balance in the hydrophobic environment was affected by either GLY or RUP.

Evaluation of hypoglycemic and hypolipidemic activity of methanol extract of Brassica oleracea

AIM: The hypoglycemic and hypolipidemic effects of the methanol extract of Brassica oleracea var. capitata(MEB) was evaluated in alloxan-induced diabetic rabbits. METHOD: The study was conducted on twenty-eight healthy white rabbits of either sex. All animals were equally divided into four groups. After confirmation of hyperglycemia, the animals of the treated and standard groups were administered MEB(500 mg·kg-1) and glibenclamide(10 mg·kg-1), respectively for 15 and 30 days. The animals of the normal and diabetic controls received normal saline 1 mL/day equivalent to the volume of doses given to the test and standard animals. Biochemical tests were performed at the end of dosing, i.e. the 16 th and 31 st days. RESULTS: The MEB revealed a decrease of 106.6 mg·dL-1 in fasting blood glucose as compared to diabetic control, which was almost comparable to glibenclamide; both of these changes were highly significant. The decrease in total cholesterol and low density lipoprotein was 94.3 and 96.5 mg·dL-1, respectively, whereas the high-density lipoprotein was increased by 26.7 mg·dL-1, as compared to diabetic control. All of the changes in lipid profile were statistically significant. CONCLUSION: These results suggest the potential of MEB as a hypoglycemic and hypolipidemic agent.

Lactobacillus plantarum KLDS1.0386 with antioxidant capacity ameliorates the lipopolysaccharide-induced acute liver injury in mice by NF-κB and Nrf2 pathway

The Lactobacillus is of great interest as a natural antioxidant to ameliorate liver injury for its antioxidant capacity.In a previous experiment,Lactobacillus plantarum KLDS 1.0386 was excellent in adhesion and anti-inflammation.This research assessed the antioxidant capacity of L.plantarum KLDS 1.0386 and how it ameliorates the LPS-induced acute liver injury.In vitro results showed that L.plantarum KLDS 1.0386 had potential antioxidant capacity with 27 genes,and it had better capacities in hydroxyl radicals scavenging and lipid antioxidant than the reference strain L.plantarum WCFS1 (P < 0.05).In vivo results,L.plantarum KLDS 1.0386 significantly reduced hepatic tissue injury,pro-inflammatory cytokines (TNF-α,IL-6),myeloperoxidase (MPO) activities and biochemical indicators of hepatotoxicity (ALT,AST,ALP) (P < 0.05),compared with the LPS-treated mice.Moreover,L.plantarum KLDS 1.0386 increased the relative abundance of the genera,including Lachnospiraceae_NK4A136_group,Muribaculaceae and Alistipes,which significantly increased the level of acetic acid and butyric acid (P < 0.05).The results also showed L.plantarum KLDS 1.0386 significantly adjusted the expression of HO-1,NQO1,CAT,NF-κB,TLR4 and IκB-α in the liver (P < 0.05).Overall,these results indicated that L.plantarum KLDS 1.0386 had great functionality in reducing LPS-induced acute liver injury.