Effect of antiatherogenic high density lipoprotein (HDL) and apolipoprotein AI (apo AI) on production of prostaglandin E2 (PGE2 ) by human m onocyte- derived macrophages was investigated.Macrophages were loaded with...Effect of antiatherogenic high density lipoprotein (HDL) and apolipoprotein AI (apo AI) on production of prostaglandin E2 (PGE2 ) by human m onocyte- derived macrophages was investigated.Macrophages were loaded with acetylated low density lipoprotein followed by incuba- tion with HDL3or apo AI.PGE2 produced and secreted in culture supernatant was quantified by en- zyme im munoassay.HDL3induced production of PGE2 by m acrophages in a time- dependent m an- ner.2 4 h after incubation,PGE2 production by HDL3- treated macrophages increased 3.7- fold of that by control cells.Apo AI also induced PGE2 secretion to 2 .1- fold,which was significantly less than HDL3.The data indicate that both HDL3and lipid- free apo AI enhance PGE2 synthesis and se- cretion by hum an m acrophages and this may further contribute to the protection from atheroscle- rosis.展开更多
OBJECTIVE: To study the efficacy and explore the mechanism of the anti-tumor immunity elicited by heat shock protein 70-peptide complexes (HSP70-PC) derived from tumor cells. METHODS: Cells culture, flow cytometric an...OBJECTIVE: To study the efficacy and explore the mechanism of the anti-tumor immunity elicited by heat shock protein 70-peptide complexes (HSP70-PC) derived from tumor cells. METHODS: Cells culture, flow cytometric analysis, affinity chromatography for protein purification, SDS-PAGE, Western-blotting and animal experiment were used. RESULTS: HSP70-PC immunization rendered protective effect to both naive tumorl-bearing mice. All of the naive mice obtained complete resistance to Hcaf cell attack; 40% of the tumor-bearing mice survived for over 90 days, whereas the mice of control group died within 2 weeks (P展开更多
文摘Effect of antiatherogenic high density lipoprotein (HDL) and apolipoprotein AI (apo AI) on production of prostaglandin E2 (PGE2 ) by human m onocyte- derived macrophages was investigated.Macrophages were loaded with acetylated low density lipoprotein followed by incuba- tion with HDL3or apo AI.PGE2 produced and secreted in culture supernatant was quantified by en- zyme im munoassay.HDL3induced production of PGE2 by m acrophages in a time- dependent m an- ner.2 4 h after incubation,PGE2 production by HDL3- treated macrophages increased 3.7- fold of that by control cells.Apo AI also induced PGE2 secretion to 2 .1- fold,which was significantly less than HDL3.The data indicate that both HDL3and lipid- free apo AI enhance PGE2 synthesis and se- cretion by hum an m acrophages and this may further contribute to the protection from atheroscle- rosis.
文摘OBJECTIVE: To study the efficacy and explore the mechanism of the anti-tumor immunity elicited by heat shock protein 70-peptide complexes (HSP70-PC) derived from tumor cells. METHODS: Cells culture, flow cytometric analysis, affinity chromatography for protein purification, SDS-PAGE, Western-blotting and animal experiment were used. RESULTS: HSP70-PC immunization rendered protective effect to both naive tumorl-bearing mice. All of the naive mice obtained complete resistance to Hcaf cell attack; 40% of the tumor-bearing mice survived for over 90 days, whereas the mice of control group died within 2 weeks (P
