Objective To identify the novel species ‘Mycobacterium fukienense＇ sp. nov of Mycobacterium chelonoe/abscessus complex from tuberculosis patients in Fujian Province, China. Methods Five of 27 clinical Mycobucterium ...Objective To identify the novel species ‘Mycobacterium fukienense＇ sp. nov of Mycobacterium chelonoe/abscessus complex from tuberculosis patients in Fujian Province, China. Methods Five of 27 clinical Mycobucterium isolates （CIs） were previously identified as M. chelonoe/obscessus complex by sequencing the hsp65, rpoB, 165-235 rRNA internal transcribed spacer region （its）, recA and sodA house-keeping genes commonly used to describe the molecular characteristics of Mycobocterium. Clinical Mycobecterium isolates were classified according to the gene sequence using a clustering analysis program. Sequence similarity within clusters and diversity between clusters were analyzed. Results The 5 isolates were identified with distinct sequences exhibiting 99.8% homology in the hsp65 gene. However, a complete lack of homology was observed among the sequences of the rpoB, 165-235 rRNA internal tronscribed spacer region （its）, sodA, and recA genes as compared with the M. obscessus. Furthermore, no match for rpoB, sodA, and recA genes was identified among the published sequences. Conclusion The novel species, Mycobacterium fukienense, is identified from tuberculosis patients in Fujian Province, China, which does not belong to any existing subspecies of M. cheloneo/abscessus complex.展开更多
目的分析深圳地区龟分枝杆菌和脓肿分枝杆菌耐药谱，评价E试验法检测龟分枝杆菌和脓肿分枝杆菌药物敏感性的临床适用性。方法用Geno Type Mycobacterium CM分枝杆菌菌种鉴定系统筛选16株龟分枝杆菌临床菌株、20株脓肿分枝杆菌临床菌株...目的分析深圳地区龟分枝杆菌和脓肿分枝杆菌耐药谱，评价E试验法检测龟分枝杆菌和脓肿分枝杆菌药物敏感性的临床适用性。方法用Geno Type Mycobacterium CM分枝杆菌菌种鉴定系统筛选16株龟分枝杆菌临床菌株、20株脓肿分枝杆菌临床菌株，分别用微量肉汤稀释法和E试验法检测其对阿米卡星、头孢西丁、环丙沙星、克拉霉素、多西环素、亚胺培南、利奈唑胺、磺胺甲嗯唑／甲氧苄啶和妥布霉素9种抗生素的MIC，以微量肉汤稀释法为标准，评价E试验法的MIC值符合率和MIC解释的一致率。两组间率的比较采用，检验。结果龟分枝杆菌和脓肿分枝杆菌对阿米卡星和头孢西丁均敏感（36／36），对克拉霉素的耐药率较低（1／36），对环丙沙星、多西环素、亚胺培南和磺胺甲嗯唑／甲氧苄啶的耐药率较高（29／36）。龟分枝杆菌和脓肿分枝杆菌对利奈唑胺的耐药例数分别为2／16和12／20，对妥布霉素的耐药例数分别为7／16和16／20。E试验法检测9种抗生素的MIC值分布与微量肉汤稀释法MIC值分布的总体符合率为46％（149／324），MIC解释一致率最高的分别为阿米卡星、克拉霉素、多西环素和亚胺培南（35／36），其次为磺胺甲嗯唑／甲氧苄啶（34／36），2例（2／36）解释结果有重大误差；一致率较高的为环丙沙星（31／36）和妥布霉素（26／36）；一致率最低的为头孢西丁和利奈唑胺（14／36），易导致MIC解释出现假阳性。结论阿米卡星、头孢西丁和克拉霉素对龟分枝杆菌和脓肿分枝杆菌有良好的体外抗菌作用，利奈唑胺、妥布霉素对部分龟分枝杆菌有良好的体外抗菌作用。除头孢西丁和利奈唑胺外，阿米卡星、克拉霉素、多西环素、环丙沙星和妥布霉素均适用E试验法检测龟分枝杆菌和脓肿分枝杆菌的体外药物敏感性，需慎重采用E试验法检测磺胺甲嗯唑／甲氧苄啶的药物敏感性。展开更多
基金supported by the National Key Programme of Mega Infectious Diseases (2008ZX100/03-010-02)the National Natural Science Funding of China (30800029)
文摘Objective To identify the novel species ‘Mycobacterium fukienense＇ sp. nov of Mycobacterium chelonoe/abscessus complex from tuberculosis patients in Fujian Province, China. Methods Five of 27 clinical Mycobucterium isolates （CIs） were previously identified as M. chelonoe/obscessus complex by sequencing the hsp65, rpoB, 165-235 rRNA internal transcribed spacer region （its）, recA and sodA house-keeping genes commonly used to describe the molecular characteristics of Mycobocterium. Clinical Mycobecterium isolates were classified according to the gene sequence using a clustering analysis program. Sequence similarity within clusters and diversity between clusters were analyzed. Results The 5 isolates were identified with distinct sequences exhibiting 99.8% homology in the hsp65 gene. However, a complete lack of homology was observed among the sequences of the rpoB, 165-235 rRNA internal tronscribed spacer region （its）, sodA, and recA genes as compared with the M. obscessus. Furthermore, no match for rpoB, sodA, and recA genes was identified among the published sequences. Conclusion The novel species, Mycobacterium fukienense, is identified from tuberculosis patients in Fujian Province, China, which does not belong to any existing subspecies of M. cheloneo/abscessus complex.
文摘目的分析深圳地区龟分枝杆菌和脓肿分枝杆菌耐药谱，评价E试验法检测龟分枝杆菌和脓肿分枝杆菌药物敏感性的临床适用性。方法用Geno Type Mycobacterium CM分枝杆菌菌种鉴定系统筛选16株龟分枝杆菌临床菌株、20株脓肿分枝杆菌临床菌株，分别用微量肉汤稀释法和E试验法检测其对阿米卡星、头孢西丁、环丙沙星、克拉霉素、多西环素、亚胺培南、利奈唑胺、磺胺甲嗯唑／甲氧苄啶和妥布霉素9种抗生素的MIC，以微量肉汤稀释法为标准，评价E试验法的MIC值符合率和MIC解释的一致率。两组间率的比较采用，检验。结果龟分枝杆菌和脓肿分枝杆菌对阿米卡星和头孢西丁均敏感（36／36），对克拉霉素的耐药率较低（1／36），对环丙沙星、多西环素、亚胺培南和磺胺甲嗯唑／甲氧苄啶的耐药率较高（29／36）。龟分枝杆菌和脓肿分枝杆菌对利奈唑胺的耐药例数分别为2／16和12／20，对妥布霉素的耐药例数分别为7／16和16／20。E试验法检测9种抗生素的MIC值分布与微量肉汤稀释法MIC值分布的总体符合率为46％（149／324），MIC解释一致率最高的分别为阿米卡星、克拉霉素、多西环素和亚胺培南（35／36），其次为磺胺甲嗯唑／甲氧苄啶（34／36），2例（2／36）解释结果有重大误差；一致率较高的为环丙沙星（31／36）和妥布霉素（26／36）；一致率最低的为头孢西丁和利奈唑胺（14／36），易导致MIC解释出现假阳性。结论阿米卡星、头孢西丁和克拉霉素对龟分枝杆菌和脓肿分枝杆菌有良好的体外抗菌作用，利奈唑胺、妥布霉素对部分龟分枝杆菌有良好的体外抗菌作用。除头孢西丁和利奈唑胺外，阿米卡星、克拉霉素、多西环素、环丙沙星和妥布霉素均适用E试验法检测龟分枝杆菌和脓肿分枝杆菌的体外药物敏感性，需慎重采用E试验法检测磺胺甲嗯唑／甲氧苄啶的药物敏感性。
