Inhibitory effect of Patrinia scabiosaefolia on acute pancreatitis

AIM： To investigate the effect of Patrinia scabiosaefolia （PS） on the cholecystokinin （CCK） octapeptide-induced acute pancreatitis （AP） in rats.METHODS： Wistar rats weighing 240-260 g were divided into three groups： （1） Normal saline-treated group; （2） treatment with PS at 100 mg/kg group, in which PS was administered orally, followed by subcutaneous administration of 75μg/kg CCK octapeptide three times after 1, 3 and 5 h, and this whole procedure was repeated for 5 d; （3） treatment with saline group, in which the protocols were the same as in treatment group with PS. We determined the pancreatic weight/ body weight ratio, the levels of pancreatic HSP60, HSP72 and the secretion of pro-inflammatory cytokines. Repeated CCK octapeptide treatment resulted in the typical laboratory findings of experimentally induced pancreatitis.RESULTS： PS reduced the pancreatic weight/body weight ratio, the levels of serum amylase and lipase, and inhibited expressions of pro-inflammatory cytokines in the CCK octapeptide-induced AP. Furthermore, PS pretreatment increased the pancreatic levels of HSP60 and HSP72.CONCLUSION： Pretreatment with PS has an antiiinflammatory effect on CCK octapeptide-induced AP.

Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression

Patrinia scabiosaefolia,is used as wild vegetable in China for more than 2000 years,with a variety of pharmacological activities,including anti-inflammatory,anti-tumor and hypoglycemic.Based on our ongoing research on chemical constituents and hypoglycemic activity of P.scabiosaefolia,4 lignan compounds,(+)-isolariciresinol(1),7R,7’R,8S,8’S-(+)-neo-olivil-4-O-β-D-glucopyranoside(2),4-O-methylcedrusin(3)and patrinian A(4),were isolated and identifi ed.The hypoglycemic activity showed that compounds 2 and 3 could extremely signifi cantly improve insulin resistance at 100(P<0.001),50(P<0.001)and 25μmol/L(P<0.01)in IR 3T3-L1 cells.While compound 4 only promoted glucose uptake by IR 3T3-L1 cells at 100μmol/L(P<0.01).Western blotting experiments showed that compounds 2 and 4 up-regulated the protein expressions of p-IRS,PI-3K,p-AKT and glucose transporter 4(GLUT4),and promoted the transcription of GLUT4 mRNA.Therefore,the mechanisms of compounds 2 and 4 were presumed to improve IR by activating PI-3K/AKT signaling pathway.

Chemical Composition,Anticancer,Anti-neuroinflammatory,and Antioxidant Activities of the Essential Oil of Patrinia scabiosaefolia

Objective：To study the chemical composition,anticancer,anti-neuroinflammatory,and antioxidant activities of the essential oil of Patrinia scabiosaefolia（EO-PS）.Methods：Patrinia scabiosaefolia was analyzed by gas chromatography-mass spectrometry.Eight human carcinoma cell lines,including SGC-7901,AGS,Hep G2,HT-29,HCT-8,5-FU/HCT-8,He La,and MDA-MB-231,were assessed by methylthiazolyldiphenyltetrazolium bromide（MTT） assay.Anti-neuroinflammatory activity was assessed by production of interleukin（IL）-1β and IL-6 induced by lipopolysaccharide in BV-2 cells（microglia from mice）.The antioxidant activity was evaluated with a 2,2-diphenyl-1-picrylhydrazyl（DPPH） scavenging assay.Results：Forty-four components,representing 83.919% of the total oil,were identified in the EO-PS.The major constituents were caryophyllene oxide（12.802%）,caryophyllene（6.909%）,α-caryophyllene（2.927%）,β-damascenone（3.435%）,calarene（5.621%）,and phenol（3.044%）.The MTT assay showed that the EO-PS exhibited significant dose-dependent growth inhibition in the 50–200 μg/m L dilution range.The EO-PS exhibited a dose-dependent scavenging activity against the DPPH radical,with an half of maximal inhibitory concentration 1.455 mg/m L.Conclusion：The EO-PS possesses a wide range of antitumor,anti-neuroinflammatory and antioxidant activities,suggesting that it may be a good candidate for further investigations of new bioactive substances.

Patrinia scabiosaefolia Inhibits Growth of 5-FU-Resistant Colorectal Carcinoma Cells via Induction of Apoptosis and Suppression of AKT Pathway

Objective: To investigate the effects of ethanol extract of Patrinia scabiosaefolia(EEPS) on chemo-resistance of colorectal cancer cells(CRC) and explore the possible molecular mechanisms. Methods: 5-fluorouracil(5-FU)-resistant human colorectal carcinoma cell line(HCT-8/5-FU) and its parental cells HCT-8 were treated with EEPS(0, 0.25, 0.50, 1 or 2 mg/mL), or 5-FU(0, 100, 200, 400, 800 or 1600 μmol/L). The 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay was performed to evaluate the cell viability. Cell density was observed by phase-contrast microscope, cell counting and colony formation assay were used to determine the cell proliferation of HCT-8/5-FU cells treated with 0, 0.5, 1 or 2 mg/mL EEPS. Cell apoptosis was determined by Hoechst staining. Western-blot was performed to detect the phosphorylation of AKT as well as the protein expression level of B-cell CLL/lymphoma 2(Bcl-2) and Bcl-2-associated X protein(Bax). Results: Compared with HCT-8 cells, MTT assay results indicated that HCT-8/5-FU cells were resistant to 5-FU treatment(P<0.05), and sensitive to EEPS treatment(P>0.05). Moreover, compared with untreated HCT-8/5-FU cells, 1 and 2 mg/mL of EEPS treatment significantly reduced cell density, cell number, inhibited cell survival(P<0.05), and induced apoptosis in HCT-8/5-FU cells. Furthermore, 1 and 2 mg/mL of EEPS significantly decreased the phosphorylation level of p-AKT and Bcl-2 protein expression, and increased the expression of Bax protein(P<0.05). Conclusion: EEPS is a promising therapeutic agent that may overcome chemo-resistance in cancer cells, likely through suppression of the AKT pathway and promotion of cancer cell apoptosis.

败酱草水提物通过调控circ_0000936/miR-665抑制胶质瘤细胞增殖、迁移及侵袭

目的:探讨败酱草水提物对胶质瘤细胞生物行为的影响及其可能作用机制。方法:收集2019年2月至2020年1月期间于本院接受手术切除并经病理证实的31例胶质瘤组织,qRT-PCR法检测正常脑组织与胶质瘤组织中circ_0000936、miR-665的表达量;Pearson法分析胶质瘤组织中circ_0000936和miR-665表达量的相关性;体外培养人胶质瘤细胞LN229,不同浓度的败酱草(0.5 mg/mL、1.0 mg/mL、2.0 mg/mL)处理LN229细胞,si-NC、si-circ_0000936分别转染至LN229细胞,pcDNA-circ_0000936转染至LN229细胞后加入2.0 mg/mL败酱草处理细胞;CCK-8、平板克隆形成实验、Transwell小室实验与流式细胞术分别检测细胞增殖、克隆形成、迁移、侵袭及凋亡能力;双荧光素酶报告基因实验检测circ_0000936与miR-665的靶向关系;Western blot法检测Bax、Bcl-2蛋白表达量。结果:胶质瘤组织中circ_0000936的表达量高于正常脑组织(P<0.05),而miR-665的表达量低于正常脑组织(P<0.05);circ_0000936和miR-665呈负相关(r=-0.980,P<0.001);败酱草能够以浓度依赖性方式降低细胞活力、细胞克隆形成率、Bcl-2蛋白水平和circ_0000936的表达量(P<0.05),迁移及侵袭细胞数减少(P<0.05),而凋亡率、Bax蛋白水平和miR-665的表达量升高(P<0.05);转染si-circ_0000936后miR-665的表达量升高(P<0.05),细胞活力、细胞克隆形成率和Bcl-2蛋白水平降低(P<0.05),迁移及侵袭细胞数减少(P<0.05),凋亡率和Bax蛋白水平升高(P<0.05);转染pcDNA-circ_0000936能够逆转败酱草对LN229细胞生物学行为的作用。结论:败酱草水提物可通过调控circ_0000936/miR-665诱导胶质瘤细胞凋亡,并阻碍增殖、迁移及侵袭。

黄花败酱的化学成分研究

目的 :对黄花败酱的化学成分进行研究。方法 :采用乙醇提取、溶剂萃取、硅胶柱层析、重结晶等从败酱科植物黄花败酱的全草中提取分离活性成分 ,通过波谱解析及化学方法进行结构鉴定。结果 :自黄花败酱醇提物中分得 8个化合物。结论 :除文献报道过的齐墩果酸 (oleanolic acid)、东莨菪素 (scopoletin)、β-谷甾醇 (β-sitosterol )和β-胡萝卜甙 (daucostarine)外 ,还首次从黄花败酱中分到 2α-羟基齐墩果酸 (2α-hydroxyoleanolicacid)、2α-羟基乌苏酸 (2α-hydroxyursolic acid)、齐墩果酸 -3 -O-β-D-吡喃木糖苷 (oleanolic acid-3 -O-β-D -xylopy-ranoside )和 3 ,4-二羟基苯甲酸 (3 ,4-dihydroxybenzoic acid )

黄花败酱中酰化新皂苷的分离与鉴定

目的 研究黄花败酱根及根茎的化学成分。方法 用大孔树脂纯化 ,硅胶柱层析和高效液相层析分离 ,根据化学反应和 U V、IR、NMR(1 H、1 3C、DEPT、HMQC、HMBC)光谱数据分析鉴定了结构。结果 从黄花败酱根与根茎的丙酮提取液中分离出 1个化合物 ,鉴定为 :常春藤皂苷元 - 3- O-β- D-葡萄吡喃糖基 (1→ 3′) - (2′- O-乙酰基 ) -α- L -阿拉伯吡喃糖苷。结论 此化合物是一个新的皂苷类化合物。

黄花败酱中一个三萜酯甙成分的分离与鉴定

从黄花败酱根和根茎中分离出一个三萜酯甙类化合物,经化学反应和光谱分析鉴定为常春藤皂甙元28-O-β-D-葡萄吡喃糖基-(1→6)-β-D-葡萄吡喃糖酯甙[28-O-β-D-glucopyranosyl-(1→6)-β-D-glu-copyranosyl-hederagenin ester],为首次从本属植物中分得。

黄花败酱中一个皂甙的分离鉴定

从黄花败酱根和根茎的丙酮提取物中分离出一个皂甙类化合物,经化学反应和光谱分析鉴定为齐墩果酸-3-O-α-L鼠李吡喃糖基1→2-β-D-木吡喃糖甙,即giganteaside D,并通过2D-NMR谱对其部分碳谱数据进行了修正。此化合物为首次从本属植物中获得。

黄花败酱黄酮类化合物的提取与分析

用70%乙醇提取黄花败酱黄酮类化合物,得到粗黄酮,经聚酰胺纯化,得到精制黄酮。结果表明:黄花败酱干粉中总黄酮含量为2.35%,粗黄酮、精制黄酮中黄酮含量分别为8.81%和21.39%。黄花败黄酮类化合物主要是黄酮和黄酮醇两类,其中含有芦丁成分。

黄花败酱超临界萃取物的化学成分研究

目的研究黄花败酱超临界萃取物的化学成分。方法采用CO2超临界萃取法提取黄花败酱中的化学成分,用GC-MS法对其中的挥发性成分进行了定性鉴别和相对含量测定。结果从黄花败酱的CO2超临界萃取物中共鉴定出41个化合物。结论黄花败酱的CO2超临界萃取物中主要含有单萜、倍半萜及其它们的含氧衍生物和脂肪酸及其酯。

黄花败酱中齐墩果酮酸的分离鉴定

从黄花败酱根和根茎的丙酮提取物中分离得到1个单体化合物,经化学反应和光谱分析鉴定为齐墩果酮酸,此化合物为首次从本属植物中获得。

黄花败酱草对小鼠肝癌细胞血道转移的影响

目的探讨黄花败酱草对肝癌血道转移小鼠的影响。方法小鼠尾静脉接种H22肝癌细胞悬液,建立血道转移模型,用黄花败酱草水提液干预,观察小鼠体重、免疫器官和肺部转移瘤生长情况。结果黄花败酱草作用的小鼠,肺部转移灶数较对照组明显减少,体重和免疫器官重量明显增加。结论黄花败酱草具有一定的抑制小鼠H22肝癌血道转移的作用。

黄花败酱总皂苷胶囊中齐墩果酸的含量测定

目的建立一种黄花败酱总皂苷胶囊质量控制的定量方法。方法采用薄层色谱扫描法。以石油醚-醋酸乙酯(3∶1)为展开剂,λS=530nm,参比波长λR=700nm,SX=3。结果此系统分离效果好,斑点前后无杂质干扰,适于定量分析。黄花败酱胶囊中含齐墩果酸1.655mg/g。结论该方法灵敏、简便、准确,可用于该制剂的含量测定。

GC-MS法检测白花败酱草与黄花败酱草挥发性成分

利用气相色谱-质谱联用(GC-MS)分析白花败酱草与黄花败酱草中挥发性成分.采用水蒸气蒸馏法提取败酱草中挥发性成分,利用气质联用技术对成分进行分析并应用NIST 05a.L标准质谱图库检索鉴定各成分、采用面积归一化法计算各成分的相对百分含量.结果表明:黄花败酱草挥发油中鉴定54个成分,占总量的94.56%.白花败酱草挥发油中共鉴定36种成分,占总量的89.87%.两种挥发油共同含有成分9种,分别为hexanal,2-Pentylfuran,benzeneacetaldehyde,α-Ionene,1,2,3,4-Tetrahydro-1,6,8-trimthylnaphthalene,1,2,3,4-Tetrahydro-1,5,7-trimethylnaphthalene,β-Damascenone,hexahydrofarnesylacetone,palmitic acid.首次对白花败酱草与黄花败酱草挥发性成分进行对比分析,为白花败酱草与黄花败酱草的鉴别、质量评价以及挥发性成分开发利用提供基础.

黄花败酱中三萜皂苷类成分的分离鉴定

目的研究黄花败酱中的化学成分。方法将黄花败酱全草的乙醇提取物经大孔树脂、硅胶、ODS柱分离得到6个化合物,通过(1H、13CNMR、ESI-MS等分析和化学方法进行结构鉴定。结果6个化合物分别被鉴定为3-O-β-D-吡喃木糖-(1→3)-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖-常春藤皂苷元-28-O-β-D-吡喃葡萄糖(6→1)-β-D-吡喃葡萄糖-(4→1)-α-L-吡喃鼠李糖酯苷(Ⅰ)、3-O-β-D-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖-常春藤皂苷元-28-O-β-D-吡喃葡萄糖(6→1)-β-D-吡喃葡萄糖-(4→1)-α-L-吡喃鼠李糖酯苷(Ⅱ)、齐墩果酸-3-α-L-吡喃阿拉伯糖-(2→1)-α-L-吡喃鼠李糖-(3→1)-O-β-D-吡喃木糖苷(Ⅲ)、3-O-β-D-吡喃木糖-(1→3)-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖-齐墩果酸-28-O-β-D-吡喃葡萄糖酯苷(Ⅳ)、齐墩果酸-3-O-β-D-吡喃木糖-(2→1)-α-L-吡喃鼠李糖苷(Ⅴ)、常春藤皂苷元-3-O-α-L-吡喃阿拉伯糖-(2→1)-α-L-吡喃鼠李糖苷(Ⅵ)。结论化合物Ⅳ为一个新的天然产物,化合物Ⅰ和Ⅲ为首次从该植物中分离得到。

超声波提取法和索氏提取法提取败酱草中齐墩果酸含量的比较

采用超声波提取法和索氏提取法提取,用高效液相色谱技术,分别测定败酱草中齐墩果酸的含量。结果表明超声波提取法提取败酱草中齐墩果酸优于索氏提取法。

黄花败酱草挥发性化学成分分析

用水蒸气蒸馏法提取黄花败酱草挥发油,并用气相色谱-质谱联用仪对黄花败酱草挥发油的化学成分进行了分离和鉴定.分离并鉴定出28个组分,占峰面积的97.21%,并用峰面积归一化法测定了各成分的质量分数;其主要挥发性成分为呋喃类化合物、酸类化合物和醇类化合物.

败酱草药材高效液相色谱指纹图谱鉴别研究

目的用高效液相色谱法对不同品种的败酱草药材进行指纹图谱的鉴别研究。方法采用Kromasil-C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸,流速0.8 mL/min,柱温25℃,检测波长320 nm。结果两种败酱草药材的相似度仅有0.021。结论高效液相色谱法可用来鉴别白花败酱和黄花败酱。

黄花败酱不同居群的形态多样性分析

目的为黄花败酱的野外调查、收集提供形态学依据。方法以黄花败酱2个居群、每个居群30个单株为材料,对2个形态学性状的多样性进行了分析。结果 2个居群总遗传多样性指数为2.022,遗传多样性主要集中于居群内(96.44%),而居群间的遗传变异(3.56%)相对较小;不同取样梯度下遗传多样性指数随单株取样数目的增加呈现增大趋势,但当取样数目达到22株时,遗传多样性指数达到最高值。结论对黄花败酱野外调查、收集时,应以居群为单位,而且每一居群至少应调查、收集22个单株,才能代表居群的遗传多样性。