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Combinations of Right Half-Plane Mappings and Vertical Strip Mappings Convex in the Vertical Direction
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作者 Yulin Cai Zirun She +1 位作者 Fanji Zeng Lu Huang 《Open Journal of Statistics》 2023年第6期941-954,共14页
In this paper, we explore the linear combinations of right half-plane mappings and vertical strip mappings. We demonstrate that the combinations of these harmonic mappings are convex in the vertical direction provided... In this paper, we explore the linear combinations of right half-plane mappings and vertical strip mappings. We demonstrate that the combinations of these harmonic mappings are convex in the vertical direction provided they are locally univalent and sense-preserving. Furthermore, we extend this analysis to a more general case by setting specific conditions. Additionally, we take some common parameters such as  as the dilatation of these harmonic mappings, and prove the sufficient conditions that their combinations are locally univalent and convex in the vertical direction. Several examples are constructed by the Mathematica software to demonstrate our main results. 展开更多
关键词 Harmonic mapping Linear Combination Cohn’s Rules Directional Convexi-ty
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基于多目标遗传算法的8×8 S盒的优化设计方法
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作者 王永 王明月 龚建 《西南交通大学学报》 EI CSCD 北大核心 2024年第3期519-527,538,共10页
混沌系统具有非线性、伪随机性、初始值敏感等特性,为基于动力系统构造性能良好的S盒提供了基础,进一步保证了分组加密算法安全性.目前,基于混沌构造S盒的方法大多数针对单个性能指标进行优化,难以获得全面的性能提升.针对此问题,结合... 混沌系统具有非线性、伪随机性、初始值敏感等特性,为基于动力系统构造性能良好的S盒提供了基础,进一步保证了分组加密算法安全性.目前,基于混沌构造S盒的方法大多数针对单个性能指标进行优化,难以获得全面的性能提升.针对此问题,结合混沌映射与多目标遗传算法,提出了一种新的S盒设计方法.首先,利用混沌映射的特性产生初始S盒种群;然后,以S盒的非线性度和差分均匀性为优化目标,基于遗传算法框架对上述两指标进行优化.针对S盒的特点,在优化算法中引入了交换操作,设计了新的变异操作以及非支配序集计算,有效提升了S盒的非线性度和差分均匀性.实验结果表明该算法产生的S盒其差分均匀度为6,非线性度值至少为110,有效提升了S盒的综合性能. 展开更多
关键词 s 非线性度 差分均匀度 多目标遗传算法 混沌映射
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基于改进混沌麻雀搜索算法S盒与神经元电路的图像加密技术
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作者 杨超 魏霞 王聪 《计算机应用与软件》 北大核心 2024年第10期335-341,385,共8页
鉴于目前的图像加密算法所采用的S盒结构过于简单,易被破解。选用由改进的脉冲耦合尖峰神经元电路得到的混沌映射(MPSNC),并使用该映射对混沌麻雀搜索算法(CSSA)进行优化。随后,结合MPSNC映射和改进后的CSSA,设计新型的混沌S盒。最后,基... 鉴于目前的图像加密算法所采用的S盒结构过于简单,易被破解。选用由改进的脉冲耦合尖峰神经元电路得到的混沌映射(MPSNC),并使用该映射对混沌麻雀搜索算法(CSSA)进行优化。随后,结合MPSNC映射和改进后的CSSA,设计新型的混沌S盒。最后,基于MPSNC映射和新型混沌S盒构造一种图像加密算法,为增加S盒的变化,对其进行S8变换。通过迭代混沌映射生成混沌序列,使用混沌序列和S8S盒对图像进行扩散和置乱操作,得到加密后的图像。经实验验证,该加密算法参数丰富,复杂度高,并由于S盒的应用,提高了算法的非线性度,具有良好的性能,适用于图像加密。 展开更多
关键词 s 神经元电路 混沌麻雀搜索算法 混沌映射 s8变换
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Illite Crystallinity Mapping of Very Low Grade Metamorphism of Triassic Metapelites in the Zoigê Area,Western China 被引量:6
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作者 TANG Yan SANG Longkang +4 位作者 YUAN Yanming YU Jishun ZHANG Yunpeng QI Xianmao YANG Yunlong 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2012年第1期96-105,共10页
X-ray diffraction methods for estimating the metamorphic grade of diagenetic, anchizone and epizone in metapelites are reviewed and applied to samples from a 7000 m+ borehole in western China and surface samples from... X-ray diffraction methods for estimating the metamorphic grade of diagenetic, anchizone and epizone in metapelites are reviewed and applied to samples from a 7000 m+ borehole in western China and surface samples from the surrounding Zoige area. Kiibler's illite crystallinity (IC) measurements provide more consistent results than calculated values of percentage of illite in the I/S mixed layers and percentage of I/S mixed layers. Down-borehole IC values display a typical burial metamorphic relationship between stratigraphic level and IC. A method for preparing very low grade metamorphic maps is described, and isograds plotted on a regional geological map at selected values of IC, delineating a high temperature diagenetic zone, an anchizone, and an epizone. The map shows that IC values are controlled by stratigraphic level in the north of the study area (i.e. burial metamorphism), and proximity to an igneous intrusive body in the south (i.e. contact metamorphism). 展开更多
关键词 X-ray diffraction metapelitic zone illite crystallinity percentage of illite in I/s mixed layers percentage of I/s mixed layers the metamorphic map
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Common Fixed Point Theorems of Single-valued and Set-valued Mappings in Complete,Convex Metric Spaces 被引量:1
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作者 LIANGJun-qi ZHANGZhi-hong ZHAOLing 《Chinese Quarterly Journal of Mathematics》 CSCD 2003年第3期264-270,共7页
Under the conditions of compatility or sub -c ompatility between a sigle-valued mapping and set-valued mapping, this paper d iscusses the existence of common fixed points for two set-valued mappings and a single-value... Under the conditions of compatility or sub -c ompatility between a sigle-valued mapping and set-valued mapping, this paper d iscusses the existence of common fixed points for two set-valued mappings and a single-valued mapping in complete, convex matric spaces. We extend and develop the main results. 展开更多
关键词 complete convex metric spaces compatible mappings s ub-compatible mappings common fixed point theorems
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Isolation and Identification of a Specific cDNA Mapping to the Bam HI-I2 and -LFragments within the Inverted Repeats ofUnique Long Re-gion (IRL) in the Genom e ofMarek′s Disease Herpesvirus (MDV) Oncogenic Strain Beijing-1 被引量:13
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作者 Lu Chun, Wu Jianping, Zhang Xunhai, et al. Lu Chun, Wu Jianping, Zhang Xunhai, et al. Department of Microbiology and Immunology, Nanjing Medical University, Nanjing\ 210029 Acta Universitatis Medicinalis Nanjing, 19(6):447 《The Journal of Biomedical Research》 CAS 1999年第2期73-73,共1页
Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 ... Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors. 展开更多
关键词 CDNA IRL in the Genom e ofMarek Isolation and Identification of a specific cDNA mapping to the Bam HI-I2 and LFragments within the Inverted Repeats ofUnique Long Re-gion Oncogenic strain Beijing-1 s Disease Herpesvirus LONG RE MDV
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STOCHASTIC FLOWS OF MAPPINGS 被引量:1
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作者 Zhao Qiaoling Yan Guojun 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2007年第3期343-352,共10页
In this paper, the stochastic flow of mappings generated by a Feller convolution semigroup on a compact metric space is studied. This kind of flow is the generalization of superprocesses of stochastic flows and stocha... In this paper, the stochastic flow of mappings generated by a Feller convolution semigroup on a compact metric space is studied. This kind of flow is the generalization of superprocesses of stochastic flows and stochastic diffeomorphism induced by the strong solutions of stochastic differential equations. 展开更多
关键词 Feller convolution semigroup Daniel integral stone's theorem stochastic flow of mappings.
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A NEW ITERATIVE METHOD FOR FINDING COMMON SOLUTIONS OF GENERALIZED EQUILIBRIUM PROBLEM,FIXED POINT PROBLEM OF INFINITE k-STRICT PSEUDO-CONTRACTIVE MAPPINGS,AND QUASI-VARIATIONAL INCLUSION PROBLEM 被引量:5
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作者 刘敏 张石生 《Acta Mathematica Scientia》 SCIE CSCD 2012年第2期499-519,共21页
In this article, we introduce a hybrid iterative scheme for finding a common element of the set of solutions for a generalized equilibrium problems, the set of common fixed point for a family of infinite k-strict pseu... In this article, we introduce a hybrid iterative scheme for finding a common element of the set of solutions for a generalized equilibrium problems, the set of common fixed point for a family of infinite k-strict pseudo-contractive mappings, and the set of solutions of the variational inclusion problem with multi-valued maximal monotone mappings and inverse-strongly monotone mappings in Hilbert space. Under suitable conditions, some strong convergence theorems are proved. Our results extends the recent results in G.L.Acedo and H.K.Xu [2], Zhang, Lee and Chan [8], Wakahashi and Toyoda [9], Takahashi and Takahashi [I0] and S. S. Chang, H. W. Joseph Lee and C. K. Chan [II], S.Takahashi and W.Takahashi [12]. Moreover, the method of proof adopted in this article is different from those of [4] and [12]. 展开更多
关键词 k-strict pseudo-contractive mappings generalized equilibrium problem vis-cosity approximation method variational inclusion problem multi-valuedmaximal monotone mappings s-inverse-strongly monotone mapping
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具有稚虫迁徙机制的S型自适应混沌蜉蝣算法
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作者 张恒齐 钱谦 《化工自动化及仪表》 CAS 2024年第2期262-273,共12页
针对蜉蝣优化算法(MA)全局搜索能力较弱,对子代优秀个体有效信息利用不够充分的缺点,以及在计算中后期易陷入局部收敛,进而影响优化效果的问题,提出一种具有稚虫迁徙机制的S型自适应混沌蜉蝣优化算法(S-AMA)。S-AMA算法首先采用Logisti... 针对蜉蝣优化算法(MA)全局搜索能力较弱,对子代优秀个体有效信息利用不够充分的缺点,以及在计算中后期易陷入局部收敛,进而影响优化效果的问题,提出一种具有稚虫迁徙机制的S型自适应混沌蜉蝣优化算法(S-AMA)。S-AMA算法首先采用Logistic混沌映射产生蜉蝣种群,增加算法初期种群的多样性;随后,将蜉蝣生命周期进行数学建模,并引入S型生命系数替换原有的重力惯性系数,动态调整算法探索能力和开发能力间的平衡性;最后,根据蜉蝣在缺氧环境下的种群活动,引入稚虫迁徙机制强化子代优秀个体摆脱局部最优的能力,进而更加充分地搜索最优解附近的区域,以增强算法的收敛精度。实验部分将S-AMA应用于标准函数测试集,并分别进行优化对比实验、Wilcoxon秩和检验。结果表明:与对比算法相比,S-AMA算法具有更好的寻优能力、收敛速度及鲁棒性。 展开更多
关键词 s型自适应混沌蜉蝣优化算法(s-AMA) 稚虫迁徙机制 混沌映射 s型生命系数 莱维飞行
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Pervasive Attentive Neural Network for Intelligent Image Classification Based on N-CDE’s
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作者 Anas W.Abulfaraj 《Computers, Materials & Continua》 SCIE EI 2024年第4期1137-1156,共20页
The utilization of visual attention enhances the performance of image classification tasks.Previous attentionbased models have demonstrated notable performance,but many of these models exhibit reduced accuracy when co... The utilization of visual attention enhances the performance of image classification tasks.Previous attentionbased models have demonstrated notable performance,but many of these models exhibit reduced accuracy when confronted with inter-class and intra-class similarities and differences.Neural-Controlled Differential Equations(N-CDE’s)and Neural Ordinary Differential Equations(NODE’s)are extensively utilized within this context.NCDE’s possesses the capacity to effectively illustrate both inter-class and intra-class similarities and differences with enhanced clarity.To this end,an attentive neural network has been proposed to generate attention maps,which uses two different types of N-CDE’s,one for adopting hidden layers and the other to generate attention values.Two distinct attention techniques are implemented including time-wise attention,also referred to as bottom N-CDE’s;and element-wise attention,called topN-CDE’s.Additionally,a trainingmethodology is proposed to guarantee that the training problem is sufficiently presented.Two classification tasks including fine-grained visual classification andmulti-label classification,are utilized to evaluate the proposedmodel.The proposedmethodology is employed on five publicly available datasets,including CUB-200-2011,ImageNet-1K,PASCAL VOC 2007,PASCAL VOC 2012,and MS COCO.The obtained visualizations have demonstrated that N-CDE’s are better appropriate for attention-based activities in comparison to conventional NODE’s. 展开更多
关键词 Differential equations neural-controlled DE image classification attention maps N-CDE’s
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Preoperative functional MRI localization of language areas in Chinese patients with brain tumors Validation with intraoperative electrocortical mapping
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作者 Hechun Xia Wei Huang +5 位作者 Liang Wu Hui Ma Xiaodong Wang Xuexin Chen Shengyu Sun Xiaoxiong Jia 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第20期1563-1569,共7页
Ten Chinese patients with brain tumors involving language regions were selected. Preoperative functional MRI was performed to locate Broca's or Wernicke's area, and the cortex that was essential for language functio... Ten Chinese patients with brain tumors involving language regions were selected. Preoperative functional MRI was performed to locate Broca's or Wernicke's area, and the cortex that was essential for language function was determined by electrocortical mapping. A site-by-site comparison between functional MRI and electrocortical mapping was performed with the aid of a neuronavigation device. Results showed that the sensitivity and specificity of preoperative functional MRI were 80.0% and 85.0% in Broca's area and 66.6% and 85.2% in Wemicke's area, respectively. These experimental findings indicate that functional MRI is an accurate, reliable technique with which to identify the location of Wernicke's area or Broca's area in patients with brain tumors. 展开更多
关键词 functional MRI brain tumor Chinese language brain mapping intraoperative electrocorticalmapping Wernicke's area Broca's area regeneration neural regeneration
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Characterization of FeCo Nanoparticles Reinforced Natural Rubber using Nanomechanical Mapping
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作者 XUE Xinghua WANG Zhiyong 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2018年第5期1223-1227,共5页
The morphology, nanomechanical properties and interfacial regions of natural rubber(NR) and FeCo nanoparticles composite were determined by AFM nanomechanical mapping. The results showed that the size of FeCo partic... The morphology, nanomechanical properties and interfacial regions of natural rubber(NR) and FeCo nanoparticles composite were determined by AFM nanomechanical mapping. The results showed that the size of FeCo particles was mostly from 40 to 100 nm and the FeCo nanoparticles were homogeneously dispersed in the NR bulk. The strength of NR composite increased with the FeCo nanoparticles loading. Young's modulus of NR region, FeCo region and interfacial region was measured by AFM nanomechanical tapping as 1.6 ± 0.6, 16.7 ±4.2 and 5.8 ± 1.5 MPa, respectively. The width of the interface for NR5, NR10 and NR15 was determined to be 15±8.1, 26±14.3 and 32±16.4 nm, respectively. 展开更多
关键词 FeCo nanoparticles natural rubber AFM nanomechanical mapping Young's modulus
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Fractional Snow/Non-Snow Cover Mapping through Incorporation of Thermal Band in Snow Index Design
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作者 B. C. Yadav Kamal Jain 《International Journal of Geosciences》 2017年第11期1404-1416,共13页
Substantial development has been achieved in snow cover delineations through binary mapping techniques. Continuous efforts for development and institution of methodologies in fractional snow cover mapping are steadily... Substantial development has been achieved in snow cover delineations through binary mapping techniques. Continuous efforts for development and institution of methodologies in fractional snow cover mapping are steadily conducted by the research communities. In this work, the attempts are driven towards the attainment of the same. MODIS (Moderate Resolution Imaging Spectroradiometer) images are worked upon Landsat 8 images under multivariate polynomial regression schemes utilizing corresponding count of pixels in a test region of Himachal Pradesh. 11.00 μm centered waveband is employed to develop a scheme for snow mapping followed by a qualitative and quantitative comparison with NDSI and S3 snow index where the values of correlation coefficient between fractional snow cover and index values have been obtained as 77.04%, 78.82% and 85.15% for NDSI, S3 and our scheme respectively. Exponential empirical relationships have been tried to be employed to attain improvements in prediction of snow cover followed by a test of correlation between true and theoretical fractional snow cover values. An improvement in degree of correlation is obtained over the conventional methodologies which serves for the verification of scheme employed and empirical relationship defined, collectively. The results provide a scope for improvements and investigations in the subject of fractional snow cover mapping. 展开更多
关键词 NDsI s3 FRACTIONAL sNOW mapping
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ON AN EQUATION CHARACTERIZING MULTI-CAUCHY-JENSEN MAPPINGS AND ITS HYERS-ULAM STABILITY
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作者 Anna BAHYRYCZ Krzysztof CIEPLINSKI Jolanta OLKO 《Acta Mathematica Scientia》 SCIE CSCD 2015年第6期1349-1358,共10页
In this paper, we give two characterizations of multi-Cauchy-Jensen mappings. One of them reduces the system of n equations defining these mappings to a single functional equation. We also prove, using the fixed point... In this paper, we give two characterizations of multi-Cauchy-Jensen mappings. One of them reduces the system of n equations defining these mappings to a single functional equation. We also prove, using the fixed point method, the generalized Hyers-Ulam stability of this equation. Our results generalize some known outcomes. 展开更多
关键词 multi-Cauchy-Jensen mapping (generalized) Hyers-Ulam stability Cauchy'sfunctional equation Jensen's functional equation fixed point method
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Solution of the Third Kind Boundary Value Problem of Laplace’s Equation Based on Conformal Mapping
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作者 Fuqian Wang 《Journal of Applied Mathematics and Physics》 2019年第3期536-546,共11页
In order to overcome the difficulty in solving the boundary value problem of electrostatic field with complex boundary and to give a new method for solving the third boundary value problem of Laplace’s equation, in t... In order to overcome the difficulty in solving the boundary value problem of electrostatic field with complex boundary and to give a new method for solving the third boundary value problem of Laplace’s equation, in this paper, the third boundary value problem of Laplace’s equation is studied by combining conformal mapping with theoretical analysis, the several analytical solutions of third boundary value problems of Laplace’s equation are gives, the correctness of its solution is verified through computer numerical simulation, and a new idea and method for solving the third boundary value problem of Laplace’s equation is obtained. In this paper, the boundary condition of the solving domain is changed by the appropriate conformal mapping, so that the boundary value problem on the transformed domain is easy to be solved or be known, and then the third kind boundary value of the Laplace’s equation can be solved easily;its electric potential distribution is known. Furthermore, the electric field line and equipotential line are plotted by using the MATLAB software. 展开更多
关键词 The THIRD KIND BOUNDARY Value Problem Laplace’s EQUATION CONFORMAL mapping The Electric Potential Distribution
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Research Status and Trends of Science and Technology Evaluation in China—Visual Analysis Based on Knowledge Mapping
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作者 Honggang Yang Haijing Zhang 《Intelligent Information Management》 2020年第6期199-210,共12页
<div style="text-align:justify;"> <span style="font-family:Verdana;">In order to understand the current status and future trends of China’s science and technology evaluation research, ... <div style="text-align:justify;"> <span style="font-family:Verdana;">In order to understand the current status and future trends of China’s science and technology evaluation research, we use the visual analysis tool CiteSpace to use the source journals included in the Chinese social science citations from 1998-2019 as the data source, and evaluate the domestic science and technology evaluation papers from the number of publications, the co-occurrence of authors, Institutional co-occurrence and subject clustering are analyzed. The study found that: the total amount of literature in the field of domestic science and technology evaluation is showing an overall upward trend;a relatively obvious author cooperation network has been formed, but the core author group has not yet been formed;institutional cooperation clusters have appeared, but the cooperation between institutions is still relatively scattered;The research topics focus on three aspects: science and technology evaluation index system, evaluation method and evaluation system.</span> </div> 展开更多
关键词 s&T Evaluation BIBLIOMETRICs Knowledge mapping CITEsPACE
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基于S型映射的自适应粒子群优化算法
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作者 薛文 《白城师范学院学报》 2024年第5期64-72,共9页
针对粒子群算法搜索精度不高,易陷入局部最优解的不足,提出了一种基于S型映射的自适应粒子群优化算法.首先,通过群体适应度方差策略,设定种群收敛阈值;然后,根据阈值采用S型映射自适应变异搜索策略,以提高全局探索能力;最后,根据适应值... 针对粒子群算法搜索精度不高,易陷入局部最优解的不足,提出了一种基于S型映射的自适应粒子群优化算法.首先,通过群体适应度方差策略,设定种群收敛阈值;然后,根据阈值采用S型映射自适应变异搜索策略,以提高全局探索能力;最后,根据适应值排序,采用S型映射自适应步长梯度搜索策略,以提高局部搜索能力.通过四个测试函数实验表明,该算法在收敛速度、收敛精度方面均有较大提升. 展开更多
关键词 粒子群算法 适应度方差 s型映射 变异策略 梯度策略
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Mapping of a gene for photoperiod-sensitive genic male sterility in Nongken 58s on chromosome 5 of rice
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作者 LIN Xinghua,YU Gongxin,ZHANG Duanpin,XIE Yuefeng,QIN Falan,State key laboratory in genetic improve ment of crops ,Huahong,Agri Univ,Wuhan 430070,China 《Chinese Rice Research Newsletter》 1996年第3期3-4,共2页
A photoperiod-sensitive genic male sterile (PGMS) rice was found in 1973 as a spontaneous mutant of Nongken 58, a japonica variety. Pollen fertility of Nongken 58s (N58s) is completely sterile when grown under long-da... A photoperiod-sensitive genic male sterile (PGMS) rice was found in 1973 as a spontaneous mutant of Nongken 58, a japonica variety. Pollen fertility of Nongken 58s (N58s) is completely sterile when grown under long-day conditions, whereas fertile under short-day conditions. This PGMS was found to be controlled by one or two recessive gene(s), of which one gene(pms)was linked to a marker gene(d-1) on chromosome 5. In order to identify a more precise location of the pms, we analyzed the populations of BCFand BCFof N58s//N58s/KL211(v-10, virescent) and N58s//N58s/KL520 (gh-1, gold hull). The marker genes v-10 and gh-1 are located on the flanking region of d-1. The F, plants of two crosses were fertile. The number of fertile and sterile individuals in BCFfit 展开更多
关键词 mapping of a gene for photoperiod-sensitive genic male sterility in Nongken 58s on chromosome 5 of rice GENE
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基于Google Maps API和AJAX的地震震情灾情展示系统 被引量:4
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作者 娄世平 董翔 +3 位作者 杨玉永 刘瑞峰 徐秀杰 贾荣光 《华北地震科学》 2018年第1期36-39,共4页
设计了一种B/S架构的地震震情灾情信息综合展示系统,采用Google Maps API v3实现前端WebGIS功能,通过AJAX方式分别访问震情和灾情数据源,解析数据,关联震情灾情信息并分类显示,实现了震情灾情态势的直观展示。
关键词 GOOGLE maps API AJAX B/s架构 综合展示
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TGF-β1调控MAP1S通过NER信号通路影响肺癌细胞的增殖和侵袭 被引量:1
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作者 豆亚伟 吕珊珊 +4 位作者 戴云 田伟 罗向晖 朱建飞 王宏涛 《解放军预防医学杂志》 CAS 2017年第12期1506-1510,共5页
目的探讨TGF-β1调控MAP1S通过NER信号通路影响肺癌细胞的增殖和侵袭。方法免疫组化检测肺癌和正常肺组织中TGFβ1和MAP1S的表达情况;免疫共沉淀实验检测TGFβ1和MAP1S蛋白的相互关系;MTT实验检测沉默MAP1S后肺癌细胞增殖能力的变化;Tra... 目的探讨TGF-β1调控MAP1S通过NER信号通路影响肺癌细胞的增殖和侵袭。方法免疫组化检测肺癌和正常肺组织中TGFβ1和MAP1S的表达情况;免疫共沉淀实验检测TGFβ1和MAP1S蛋白的相互关系;MTT实验检测沉默MAP1S后肺癌细胞增殖能力的变化;Transwell试验检测沉默MAP1S后肺癌细胞侵袭能力的影响;Western blotting检测沉默MAP1S后ERCC1和ERCC3蛋白的表达情况;裸鼠体内成瘤实验检测沉默MAP1S后肺癌细胞成瘤能力变化。结果与正常肺组织相比,肺癌组织中TGFβ1和MAP1S表达水平明显升高;免疫共沉淀实验表明TGFβ1和MAP1S结合为共同体;沉默MAP1S抑制肺癌A549细胞的增殖和侵袭能力、下调ERCC1和ERCC3蛋白的表达、抑制肺癌A549细胞的体内成瘤能力。结论 TGFβ1和MAP1S在肺癌中表达明显升高,同时沉默MAP1S可以抑制肺癌细胞增殖和侵袭能力,TGFβ1可以靶向MAP1S蛋白通过NER信号通路调控肺癌细胞的生物学行为。 展开更多
关键词 TGFΒ1 map1s 肺癌 Transwll NER
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