Objective: To investigate the time course of serum S-100 concentrations of patients with acute cerebral infarction,and their relation with the clinical data and the prognosis. Methods: Serum S-100 levels were serially...Objective: To investigate the time course of serum S-100 concentrations of patients with acute cerebral infarction,and their relation with the clinical data and the prognosis. Methods: Serum S-100 levels were serially determined in 36 patients with acute cerebral infarction within 12 h, at 24 h and day 2, 3, 4, 5, 7 and 10 after acute cerebral infarction and in 20 age- and sex-matched control subjects. An S-100 content assay was performed using a two-site radioimmunoassay technique. The clinical status was assessed using NIH Stroke Scale. The functional deficit at 4 weeks after acute cerebral infarction was scored using the modified Rankin scale. A cranial computed tomography was performed initially. Results: Elevated concentrations of S-100 (>0.2 μg/L) were observed in 29 of 36 patients with acute cerebral infarction,but none of the control subjects. The S-100 peak levels were at day 2 and 3 after acute cerebral infarction and were significantly high in those patients with severe neurological deficit at admission, with extensive infarction or with space-occupying effect of ischemic edema as compared with the rest of the populations. Conclusion: Serum S-100 level assay can be used as a peripheral marker of ischemic brain damage, and may be helpful for evaluation of therapeutic effects in acute ischemic stroke.展开更多
To investigate the expression and distribution of S-100 protein and CD83 in the thyroid tissues of autoimmune thyroid diseases(ATDs),and to study the role of the dendritic cells in the pathogenesis of ATDs, immunohist...To investigate the expression and distribution of S-100 protein and CD83 in the thyroid tissues of autoimmune thyroid diseases(ATDs),and to study the role of the dendritic cells in the pathogenesis of ATDs, immunohistochemical staining was used on pathological tissues of 20 patients with Hashimoto's thyroiditis(HT) and 20 patients with Graves' disease(GD) to check the expression and distribution of S-100 protein and CD83. Compared with control group(20 cases of thyroid follicular adenoma,TFA),the higher expressions of S-100 in HT(139.38±5.92 vs 59.47±11.69) and GD(119.42±14.48 vs 59.47±11.69) were observed respectively(p< 0.001).The increased positive expressions of CD83 which is known as a marker of mature and activated DCs in HT(22.58±13.96 vs 5.19±8.08) and GD(29.92±14.43 vs 5.19±8.08) were also found respectively(p<0.001). Serum TPO antibody(TPO-Ab,67.3±11.6%) and Tg antibody(Tg-Ab,59.8±10.1%) in HT were higher than those in GD(28.4±5.7%,23.1±4.9%) and TFA(6.1±3.4%,7.2±4.6%)(p<0.01).Serum TR-Ab in GD(16.3 ±5.6 U/L) was higher than those in HT(4.8±2.3 U/L) and TFA(2.5±1.2 U/L)(p<0.01).Our findings suggest that the high expression of DCs' markers may be related to the pathogenesis of HT and GD.The upregulation of both the number and the matured functions of DCs,may lead to present more antigens and to produce more auto-antibodies(such as Tg-Ab and TPO-Ab in HT,TR-Ab in GD),which may be involved in pathogenesis of the autoimmune thyroid diseases.Cellular & Molecular Immunology.2004;1(5):378-382.展开更多
目的探讨缺氧缺血性脑病(HIE)新生儿血清生物标志物神经元特异性烯醇酶(NSE)、S-100B、Tau蛋白、激活素A等指标变化及诊断效能。方法选取2020年1月至2022年2月在温州医科大学附属台州医院出生或就诊的75例HIE足月新生儿为研究对象,按照...目的探讨缺氧缺血性脑病(HIE)新生儿血清生物标志物神经元特异性烯醇酶(NSE)、S-100B、Tau蛋白、激活素A等指标变化及诊断效能。方法选取2020年1月至2022年2月在温州医科大学附属台州医院出生或就诊的75例HIE足月新生儿为研究对象,按照HIE严重程度分为轻度HIE组30例,中度HIE组25例,重度HIE组20例。选取同期本院出生的75名健康足月新生儿为对照组。检测并比较4组研究对象血清生物标志物及神经发育指标[包括新生儿神经行为(NBNA)、发育商(DQ)],采用Pearson相关分析血清生物标志物与神经发育指标的相关性,ROC曲线分析各项血清生物标志物单独或联合检测对HIE的诊断效能。结果4组研究对象血清生物标志物及神经发育指标比较,差异均有统计学意义(均P<0.05),其中轻、中、重度HIE组患儿血清NSE、S-100B、Tau蛋白、激活素A水平均明显高于对照组(均P<0.05),且轻度HIE组<中度HIE组<重度HIE组(均P<0.05);生后7 d NBNA评分、生后35周DQ、生后52周DQ均明显低于对照组(均P<0.05),且轻度HIE组>中度HIE组>重度HIE组(均P<0.05)。血清NSE、S-100B、Tau蛋白、激活素A水平与生后7 d NBNA评分均呈负相关(r=-0.779、-0.788、-0.714、-0.800,均P<0.05);与生后35周DQ均呈负相关(r=-0.662、-0.700、-0.626、-0.674,均P<0.05),与生后52周DQ均呈负相关(r=-0.636、-0.684、-0.619、-0.632,均P<0.05)。血清NSE、S-100B、Tau蛋白、激活素A单独检测和4项联合检测诊断HIE的AUC分别为0.923、0.946、0.942、0.939和0.995,4项联合检测的诊断效能最佳。结论血清NSE、S-100B、Tau蛋白、激活素A与HIE新生儿神经发育有关,4项联合检测诊断HIE的效能较高。展开更多
Objective： To observe the effects of electro-acupuncture on S-100 protein expression of nerve fibers on the regeneration process of transected sciatic nerve of rats, so as to explore the partial mechanism of electro-...Objective： To observe the effects of electro-acupuncture on S-100 protein expression of nerve fibers on the regeneration process of transected sciatic nerve of rats, so as to explore the partial mechanism of electro-acupuncture treatment for peripheral nerve injury. Methods： Following rat＇s sciatic nerve transection, the nerve regeneration chamber model was established, then the rat was given electro-acupuncture stimulation at the frequency of 5 Hz and frequency of 100 Hz, and the immunohistochemical staining method and image analyzing semi-quantitative technique were adopted to determine the percentage of S-100 protein area, hence to observethe effect of electro-acupuncture on the repair process of transected sciatic nerve of rat and the S-100 protein expression. Results： Electro-acupuncture could significantly potentiate the S-100 protein expression of Schwann Cells （SC） in the repair process of transected sciatic nerve. At the 14th week, 17th week and 20th week after operation, the percentages of S-100 protein area were more obviously increased in 5 Hz electro-acupuncture group and 100 Hz electro-acupuncture group than in model group, with significant differences （P〈0.05）. Especially, the better effect was obtained in 5 Hz electro-acupuncture group. Judging from the dynamic treatment, it is very vital to be treated early. Conclusion： Electro-acupuncture is an effective method for the treatment of peripheral nerve injury, and its therapeutic effect is related to the improvement of SC proliferation.展开更多
文摘Objective: To investigate the time course of serum S-100 concentrations of patients with acute cerebral infarction,and their relation with the clinical data and the prognosis. Methods: Serum S-100 levels were serially determined in 36 patients with acute cerebral infarction within 12 h, at 24 h and day 2, 3, 4, 5, 7 and 10 after acute cerebral infarction and in 20 age- and sex-matched control subjects. An S-100 content assay was performed using a two-site radioimmunoassay technique. The clinical status was assessed using NIH Stroke Scale. The functional deficit at 4 weeks after acute cerebral infarction was scored using the modified Rankin scale. A cranial computed tomography was performed initially. Results: Elevated concentrations of S-100 (>0.2 μg/L) were observed in 29 of 36 patients with acute cerebral infarction,but none of the control subjects. The S-100 peak levels were at day 2 and 3 after acute cerebral infarction and were significantly high in those patients with severe neurological deficit at admission, with extensive infarction or with space-occupying effect of ischemic edema as compared with the rest of the populations. Conclusion: Serum S-100 level assay can be used as a peripheral marker of ischemic brain damage, and may be helpful for evaluation of therapeutic effects in acute ischemic stroke.
文摘To investigate the expression and distribution of S-100 protein and CD83 in the thyroid tissues of autoimmune thyroid diseases(ATDs),and to study the role of the dendritic cells in the pathogenesis of ATDs, immunohistochemical staining was used on pathological tissues of 20 patients with Hashimoto's thyroiditis(HT) and 20 patients with Graves' disease(GD) to check the expression and distribution of S-100 protein and CD83. Compared with control group(20 cases of thyroid follicular adenoma,TFA),the higher expressions of S-100 in HT(139.38±5.92 vs 59.47±11.69) and GD(119.42±14.48 vs 59.47±11.69) were observed respectively(p< 0.001).The increased positive expressions of CD83 which is known as a marker of mature and activated DCs in HT(22.58±13.96 vs 5.19±8.08) and GD(29.92±14.43 vs 5.19±8.08) were also found respectively(p<0.001). Serum TPO antibody(TPO-Ab,67.3±11.6%) and Tg antibody(Tg-Ab,59.8±10.1%) in HT were higher than those in GD(28.4±5.7%,23.1±4.9%) and TFA(6.1±3.4%,7.2±4.6%)(p<0.01).Serum TR-Ab in GD(16.3 ±5.6 U/L) was higher than those in HT(4.8±2.3 U/L) and TFA(2.5±1.2 U/L)(p<0.01).Our findings suggest that the high expression of DCs' markers may be related to the pathogenesis of HT and GD.The upregulation of both the number and the matured functions of DCs,may lead to present more antigens and to produce more auto-antibodies(such as Tg-Ab and TPO-Ab in HT,TR-Ab in GD),which may be involved in pathogenesis of the autoimmune thyroid diseases.Cellular & Molecular Immunology.2004;1(5):378-382.
文摘目的探讨缺氧缺血性脑病(HIE)新生儿血清生物标志物神经元特异性烯醇酶(NSE)、S-100B、Tau蛋白、激活素A等指标变化及诊断效能。方法选取2020年1月至2022年2月在温州医科大学附属台州医院出生或就诊的75例HIE足月新生儿为研究对象,按照HIE严重程度分为轻度HIE组30例,中度HIE组25例,重度HIE组20例。选取同期本院出生的75名健康足月新生儿为对照组。检测并比较4组研究对象血清生物标志物及神经发育指标[包括新生儿神经行为(NBNA)、发育商(DQ)],采用Pearson相关分析血清生物标志物与神经发育指标的相关性,ROC曲线分析各项血清生物标志物单独或联合检测对HIE的诊断效能。结果4组研究对象血清生物标志物及神经发育指标比较,差异均有统计学意义(均P<0.05),其中轻、中、重度HIE组患儿血清NSE、S-100B、Tau蛋白、激活素A水平均明显高于对照组(均P<0.05),且轻度HIE组<中度HIE组<重度HIE组(均P<0.05);生后7 d NBNA评分、生后35周DQ、生后52周DQ均明显低于对照组(均P<0.05),且轻度HIE组>中度HIE组>重度HIE组(均P<0.05)。血清NSE、S-100B、Tau蛋白、激活素A水平与生后7 d NBNA评分均呈负相关(r=-0.779、-0.788、-0.714、-0.800,均P<0.05);与生后35周DQ均呈负相关(r=-0.662、-0.700、-0.626、-0.674,均P<0.05),与生后52周DQ均呈负相关(r=-0.636、-0.684、-0.619、-0.632,均P<0.05)。血清NSE、S-100B、Tau蛋白、激活素A单独检测和4项联合检测诊断HIE的AUC分别为0.923、0.946、0.942、0.939和0.995,4项联合检测的诊断效能最佳。结论血清NSE、S-100B、Tau蛋白、激活素A与HIE新生儿神经发育有关,4项联合检测诊断HIE的效能较高。
文摘Objective： To observe the effects of electro-acupuncture on S-100 protein expression of nerve fibers on the regeneration process of transected sciatic nerve of rats, so as to explore the partial mechanism of electro-acupuncture treatment for peripheral nerve injury. Methods： Following rat＇s sciatic nerve transection, the nerve regeneration chamber model was established, then the rat was given electro-acupuncture stimulation at the frequency of 5 Hz and frequency of 100 Hz, and the immunohistochemical staining method and image analyzing semi-quantitative technique were adopted to determine the percentage of S-100 protein area, hence to observethe effect of electro-acupuncture on the repair process of transected sciatic nerve of rat and the S-100 protein expression. Results： Electro-acupuncture could significantly potentiate the S-100 protein expression of Schwann Cells （SC） in the repair process of transected sciatic nerve. At the 14th week, 17th week and 20th week after operation, the percentages of S-100 protein area were more obviously increased in 5 Hz electro-acupuncture group and 100 Hz electro-acupuncture group than in model group, with significant differences （P〈0.05）. Especially, the better effect was obtained in 5 Hz electro-acupuncture group. Judging from the dynamic treatment, it is very vital to be treated early. Conclusion： Electro-acupuncture is an effective method for the treatment of peripheral nerve injury, and its therapeutic effect is related to the improvement of SC proliferation.
