期刊文献+
共找到30篇文章
< 1 2 >
每页显示 20 50 100
Neurotoxicity mechanism of aconitine in HT22 cells studied by microfluidic chip-mass spectrometry 被引量:6
1
作者 Yingrui Zhang Shiyu Chen +4 位作者 Fangfang Fan Ning Xu Xian-Li Meng Yi Zhang Jin-Ming Lin 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2023年第1期88-98,共11页
Aconitine,a common and main toxic component of Aconitum,is toxic to the central nervous system.However,the mechanism of aconitine neurotoxicity is not yet clear.In this work,we had the hypothesis that excitatory amino... Aconitine,a common and main toxic component of Aconitum,is toxic to the central nervous system.However,the mechanism of aconitine neurotoxicity is not yet clear.In this work,we had the hypothesis that excitatory amino acids can trigger excitotoxicity as a pointcut to explore the mechanism of neurotoxicity induced by aconitine.HT22 cells were simulated by aconitine and the changes of target cell metabolites were real-time online investigated based on a microfluidic chip-mass spectrometry system.Meanwhile,to confirm the metabolic mechanism of aconitine toxicity on HT22 cells,the levels of lactate dehydrogenase,intracellular Ca^(2+),reactive oxygen species,glutathione and superoxide dismutase,and ratio of Bax/Bcl-2 protein were detected by molecular biotechnology.Integration of the detected results revealed that neurotoxicity induced by aconitine was associated with the process of excitotoxicity caused by glutamic acid and aspartic acid,which was followed by the accumulation of lactic acid and reduction of glucose.The surge of extracellular glutamic acid could further lead to a series of cascade reactions including intracellular Ca^(2+)overload and oxidative stress,and eventually result in cell apoptosis.In general,we illustrated a new mechanism of aconitine neurotoxicity and presented a novel analysis strategy that real-time online monitoring of cell metabolites can provide a new approach to mechanism analysis. 展开更多
关键词 aconitine Neurotoxicity mechanism HT22 cells Excitatory amino acids Microfluidic chip-mass spectrometry
下载PDF
Analysis of the Aconitine Alkaloids in Chuanwu by Electrospray Ionization/Tandem Mass Spectrometry
2
作者 AiMinSUN HuiLI +2 位作者 ZhiMingHUANG P.P.H.BUT XueQinDING 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第9期1071-1074,共4页
An electrospray ionization / tandem mass spectrometric (ESI/MS/MS) method was developed for the simultaneous identification and analysis of three aconitine alkaloids [ mesacontine (MA), hypaconitine (HA), and aconitin... An electrospray ionization / tandem mass spectrometric (ESI/MS/MS) method was developed for the simultaneous identification and analysis of three aconitine alkaloids [ mesacontine (MA), hypaconitine (HA), and aconitine (A)] as intact molecules at low nanogram level in Chinese traditional medicine Chuanwu decoction as well as in human whole blood extract without chromatographic separation. 展开更多
关键词 Chuanwu aconitine masaconitine HYPaconitine ESI/MS/MS.
下载PDF
乌头及其炮制品、制剂中剧毒性成分Aconitine等含量测定的研究 被引量:7
3
作者 钱忠直 《中草药》 CAS 1982年第11期15-18,共4页
本文研究了用改良的异羟肟酸反应—高氯酸铁显色分光光度法,在波长515nm处测定乌头中剧毒的生物碱的含量。本法简便,显色稳定性好,回收率为98.5%。
关键词 标准液 aconitine 炮制品 剧毒性
下载PDF
The aconitine analog bulleyaconitine A exhibits anti-hypersensitivity through direct stimulation of dynorphin A release from spinal microglia in the rat model of neuropathy
4
《中国药理学通报》 CAS CSCD 北大核心 2015年第B11期74-74,共1页
Aim Aconitine and its structurally-related diterpenoid alkaloids have been shown to interact differential- ly with neuronal voltage-dependent sodium channels and be responsible for their analgesia and toxicity. Bulley... Aim Aconitine and its structurally-related diterpenoid alkaloids have been shown to interact differential- ly with neuronal voltage-dependent sodium channels and be responsible for their analgesia and toxicity. Bulleya- conitine A ( BAA or BLA) is an aconitine analog and has been prescribed for the management of pain. The present study aimed to evaluate the inhibitory effects of BAA on pain hypersensitivity and morphine anti-nociceptive toler- ance, and explore whether the release of dynorphin A from spinal microglia was associated with its mechanism of actions. Methods Rat models of neuropathic pain, formalin test and bone cancer pain were used, and spinal dynorphin A level and expression were measured. Sample size of animals was six in each study group. Resultes A single intrathecal or subcutaneous (but not intraventricular or local) injection of BAA blocked spinal nerve liga- tion-induced painful neuropathy, bone cancer-induced pain and formalin-induced hyperalgesia by 60% - 100% with the ED50 values of 94 - 126 ng/rat (intrathecal) and 42 - 59 μg · kg^-1 ( subcutaneous), respectively. Follow- ing chronic treatment, BAA did not induce either self-tolerance to anti-nociception or cross-tolerance to morphine anti-nociception, and completely prevented morphine tolerance. Spinal BAA anti-nociception, but not neurotoxici- ty, was completely blocked by the specific microglial inhibitor minocycline. In a minocycline-sensitive and lido- BAA stimulated the release of dynorphin A from the spinal cord, and the caine- or ropivacaine-insensitive manner, primary culture of microglia but not from neurons or astrocytes. The blockade effects of BAA on nociception and morphine tolerance were completely blocked by the specific dynorphin A antiserum and/or K-opioid receptor antago- nist. Conclusions Our results demonstrated that BAA eliminated pain hypersensitivity and morphine tolerance through the direct stimulation of dynorphin A release from spinal microglia, which was not dependent on the interac- tions with sodium channels. 展开更多
关键词 bulleyaconitine A (BAA or BLA) aconitine ANTI-NOCICEPTION pain HYPERSENSITIVITY morphine toler-ance to ANTI-NOCICEPTION SPINAL cord MICROGLIA DYNORPHIN A.
下载PDF
New Metabolites of Aconitine in Rabbit Urine 被引量:7
5
作者 HongGuiZHANG XiangGuoSHI 《Chinese Chemical Letters》 SCIE CAS CSCD 2002年第8期758-760,共3页
A sensitive analytical method to identify and determine aconitine and its metabolites in rabbit urine was developed by liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MSn).In this method,aconit... A sensitive analytical method to identify and determine aconitine and its metabolites in rabbit urine was developed by liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MSn).In this method,aconitine and its four metabolites in rabbit urine were isolated and deduced As 16-O-demethylaconine(M1), benzoylaconine(M2), 16-O-demethylbenzoylaconine (M3)and aconine(M4).M1 and M3 are new metabolites of aconitine and M2 and M4 are first identified in rabbit urine. 展开更多
关键词 ACONITUM METABOLITES aconitine LC/MS.
下载PDF
Determination of Aconitine and its Metabolites in Animal Experiment by LC/ESI-MS^n 被引量:2
6
作者 ForMaster‘sDegreeDuanMing-Yu Supervisor:Prof,ZHANGHong-gui +1 位作者 ZHONGDa-fang ZHANGHan-qi 《Journal of Chinese Pharmaceutical Sciences》 CAS 2003年第1期55-56,共2页
关键词 aconitine ACONITE METABOLITES LC/ESI MS n POISONING
下载PDF
POSTMORTEM REDISTRIBUTION KINETICS OF ACONITINE IN RABBITS 被引量:2
7
作者 徐威 胡炳蔚 《Journal of Pharmaceutical Analysis》 CAS 1994年第1期41-46,51,共7页
The postmortem redistribution of aconitine(AC) and its influencing factors by orally ingested Aconitum brachypodum Diels (AbD) in rabbits were studied. The results showed that postmortem AC redistribution did exist, a... The postmortem redistribution of aconitine(AC) and its influencing factors by orally ingested Aconitum brachypodum Diels (AbD) in rabbits were studied. The results showed that postmortem AC redistribution did exist, and the diffusion along a concentration gradient was the major influencing factor on it. Change of temperature and incomplete distribution in life also influenced it.Besides those mentioned above, there were other influencing factors. These may be related to postmortem blood movement and toxin released from cells occurring as part of the processes of autolysis and putrefaction. 展开更多
关键词 postmortem redistribution aconitine A-V concentration difference degradation Kinetics
下载PDF
Acute myocardial necrosis caused by aconitine poisoning:A case report 被引量:1
8
作者 Yu-Ping Liao Li-Han Shen +2 位作者 Li-Hua Cai Jie Chen Han-Quan Shao 《World Journal of Clinical Cases》 SCIE 2022年第33期12416-12421,共6页
BACKGROUND Herbal medicine has a long history of use in the prevention and treatment of disease and is becoming increasingly popular globally.However,there are also widespread concerns about its safety.Among them,the ... BACKGROUND Herbal medicine has a long history of use in the prevention and treatment of disease and is becoming increasingly popular globally.However,there are also widespread concerns about its safety.Among them,the cardiotoxicity of aconitine has been described.CASE SUMMARY We report a case of a 61-year-old male with aconitine poisoning presenting with malignant arrhythmia and severe cardiogenic shock,which was successfully managed with aggressive advanced life support and heart transplantation.CONCLUSION This is the first case wherein in vivo cardiac pathology was obtained,confirming that aconitine caused acute myocardial necrosis. 展开更多
关键词 aconitine ECMO Myocardial necrosis Ventricular arrhythmia Herbal medicine Case report
下载PDF
Effects of Aconitine on [Ca^(2+)] Oscillation in Cultured Myocytes of Neonatal Rats 被引量:1
9
作者 刘艳 章诗伟 +2 位作者 梁曼 刘茜 刘良 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2008年第5期499-503,共5页
In order to investigate the effects of aconitine on [Ca2+] oscillation patterns in cultured myocytes of neonatal rats, fluorescent Ca2+ indicator Fluo-4 NW and laser scanning confocal micro- scope (LSCM) were used... In order to investigate the effects of aconitine on [Ca2+] oscillation patterns in cultured myocytes of neonatal rats, fluorescent Ca2+ indicator Fluo-4 NW and laser scanning confocal micro- scope (LSCM) were used to detect the real-time changes of [Ca2+] oscillation patterns in the cultured myocytes before and after aconitine (1.0 μmol/L) incubation or antiarrhythmic peptide (AAP) and aconitine co-incubation. The results showed under control conditions, [Ca2+] oscillations were irregu- lar but relatively stable, occasionally accompanied by small calcium sparks. After incubation of the cultures with aconitine, high frequency [Ca2+] oscillations emerged in both nuclear and cytoplasmic regions, whereas typical calcium sparks disappeared and the average [Ca2+] in the cytoplasm of the cardiomyocyte did not change significantly. In AAP-treated cultures, intracellular [Ca2+] oscillation also changed, with periodic frequency, increased amplitudes and prolonged duration of calcium sparks. These patterns were not altered significantly by subsequent aconitine incubation. The basal value of [Ca2+] in nuclear region was higher than that in the cytoplasmic region. In the presence or absence of drugs, the [Ca2+] oscillated synchronously in both the nuclear and cytoplasmic regions of the same cardiomyocyte. It was concluded that although oscillating strenuously at high frequency, the average [Ca2+] in the cytoplasm of cardiomyocyte did not change significantly after aconitine incuba- tion, compared to the controls. The observations indicate that aconitine induces the changes in [Ca2+] oscillation frequency other than the Ca2+ overload. 展开更多
关键词 forensic toxicology aconitine [Ca2+] oscillation primary cell culture
下载PDF
INFLUENCE OF ETHANOL ON TOXICOKINETICS OF ACONITINE
10
作者 徐威 胡炳蔚 车向明 《Journal of Pharmaceutical Analysis》 CAS 1994年第1期52-55,76,共5页
A reverse-phase High-performance Liquid Chromatography (HPLC) method for the detection of aconitine (AC) in biological samples was used. After orally ingested Aconitum brochypodum Diels (AbD) to rabbits, the toxicokin... A reverse-phase High-performance Liquid Chromatography (HPLC) method for the detection of aconitine (AC) in biological samples was used. After orally ingested Aconitum brochypodum Diels (AbD) to rabbits, the toxicokinetics process of AC showed a two-compartment open model.Ka. A and B of which were 1.1629±0. 4053, 0. 6046±0.2574 and 1. 1607±0. 3781 mg/L respectively. The influence of ethanol on this kinetics process was studied. It was indicated that ethanol did not change the model type, but the absorption and distribution of which were improved significantly (P<0. 01), its elimination process was not influenced significantly, the values of Ka. A and B were 2. 4026 ±0. 5376, 1. 205± 0. 5328, 1. 2037 ±0. 4095 mg/L respectively. All these suggest that ethanol increases the toxicity of AbD. 展开更多
关键词 TOXICOKINETICS INTERACTION aconitine ETHANOL
下载PDF
STUDY OF ACONITINE DEGRADATION KINETICS IN RABBIT CORPSES
11
作者 徐威 胡炳蔚 +2 位作者 车向明 王多劳 多劳 《Journal of Pharmaceutical Analysis》 CAS 1996年第1期22-26,35,共6页
in order to research on the degradation kinetics rule or aconitine (AC) in rabbit corpses,in this article,through the acute intoxic models set up by orally administrating Aconitum brachypodum Diels (AbD) of absolute l... in order to research on the degradation kinetics rule or aconitine (AC) in rabbit corpses,in this article,through the acute intoxic models set up by orally administrating Aconitum brachypodum Diels (AbD) of absolute lethal dose (ALD) to New Zealand rabbits,the changing rules of aconitine degradation in tissues of rabbit corpses stored at 4℃ refrigirater were studied. The result showed that AC degradation process in rabbit corpses was apparent two-order degradation kinetics. AC degradation kinetics equations in liver and kidney were the half lives of them were 5.66 and 6. 47 days respectively. 展开更多
关键词 degradation kinetics dynamic change aconitine acute toxicity
下载PDF
Refinement of the Crystal Structure of Aconitine
12
作者 蒲小华 刘叶 +1 位作者 苟杏杏 李宗孝 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2017年第4期605-608,共4页
The crystal of aconitine has been obtained through an unexpected spontaneous change of the solid power of aconitine in air and characterized by single-crystal X-ray diffraction analysis and elemental analysis. Crystal... The crystal of aconitine has been obtained through an unexpected spontaneous change of the solid power of aconitine in air and characterized by single-crystal X-ray diffraction analysis and elemental analysis. Crystallographic data: orthorhombic, space group P2_12_12_1 with a = 17.0745(6), b = 15.5990(6), c = 12.2443(6) ?, V = 3261.2(2) ?~3, Z = 4, C_(34)H_(47)NO_(11), M_r = 645.73, D_c = 1.315 g/cm^3, F(000) = 1384, μ(MoKa) = 0.098 mm^(-1), R = 0.0605 and wR = 0.1311. Aconitine is diester diterpenoid alkaloid, and its main structure is composed of four six-membered and two five-membered rings. The intramolecular and intermolecular O–H···O and C–H···O hydrogen bonds extend the adjacent molecules into a one-dimensional chain and a two-dimensional framework, which may be the essential reason for the change. 展开更多
关键词 aconitine spontaneous change crystal structure hydrogen bonds
下载PDF
Severe aconite poisoning successfully treated with veno-arterial extracorporeal membrane oxygenation:A case report
13
作者 Saeko Kohara Yoshito Kamijo +6 位作者 Ryoko Kyan Ichiro Okada Eiju Hasegawa Soichiro Yamada Koichi Imai Asuka Kaizaki-Mitsumoto Satoshi Numazawa 《World Journal of Clinical Cases》 SCIE 2024年第2期399-404,共6页
BACKGROUND Most species of aconite contain highly toxic aconitines,the oral ingestion of which can be fatal,primarily because they cause ventricular arrhythmias.We describe a case of severe aconite poisoning that was ... BACKGROUND Most species of aconite contain highly toxic aconitines,the oral ingestion of which can be fatal,primarily because they cause ventricular arrhythmias.We describe a case of severe aconite poisoning that was successfully treated through venoarterial extracorporeal membrane oxygenation(VA-ECMO)and in which detailed toxicological analyses of the aconite roots and biological samples were performed using liquid chromatography-tandem mass spectrometry(LC-MS/MS).CASE SUMMARY A 23-year-old male presented to the emergency room with circulatory collapse and ventricular arrhythmia after ingesting approximately half of a root labeled,“Aconitum japonicum Thunb”.Two hours after arrival,VA-ECMO was initiated as circulatory collapse became refractory to antiarrhythmics and vasopressors.Nine hours after arrival,an electrocardiogram revealed a return to sinus rhythm.The patient was weaned off VA-ECMO and the ventilator on hospital days 3 and 5,respectively.On hospital day 15,he was transferred to a psychiatric hospital.The other half of the root and his biological samples were toxicologically analyzed using LC-MS/MS,revealing 244.3 mg/kg of aconitine and 24.7 mg/kg of mesaconitine in the root.Serum on admission contained 1.50 ng/mL of aconitine.Beyond hospital day 2,neither were detected.Urine on admission showed 149.09 ng/mL of aconitine and 3.59 ng/mL of mesaconitine,but these rapidly decreased after hospital day 3.CONCLUSION The key to saving the life of a patient with severe aconite poisoning is to introduce VA-ECMO as soon as possible. 展开更多
关键词 Aconite poisoning Fatal arrhythmia Veno-arterial extracorporeal membrane oxygenation aconitine MESaconitine Case report
下载PDF
Research progress of aconitine toxicity and forensic analysis of aconitine poisoning 被引量:8
14
作者 Xiangting Gao Jun Hu +3 位作者 Xincai Zhang Yuanyi Zuo Yun Wang Shaohua Zhu 《Forensic Sciences Research》 CSCD 2020年第1期25-31,共7页
Chinese herbal medicines have been extensively used in China and other countries for cen-turies.Aconitine,a diterpenoid alkaloid extracted from Aconitum plants,has anti-inflamma-tory and analgesic activities,but can a... Chinese herbal medicines have been extensively used in China and other countries for cen-turies.Aconitine,a diterpenoid alkaloid extracted from Aconitum plants,has anti-inflamma-tory and analgesic activities,but can also induce severe arrhythmia and neurotoxicity.Aconitine poisoning accidents caused by misuse,suicide,or homicide have been reported in recent years.In China,fatal aconitine poisoning can occasionally happen on account of acci-dental ingestion of some wild plants or consumption of herbal decoction made from the roots of Aconitum plants.However,it is rather difficult for forensic experts to find the specific results in present forensic autopsy of aconitine-induced death.To further clarify its potential risk following the widespread application of aconitine,toxicological characteristics and pharmacokinetics of aconitine are reviewed.Moreover,gastrointestinal,neurological,and car-diovascular symptoms were observed frequently in aconitine poisoning cases.In addition,the review also aims at providing some convincing evidences for forensic experts to identify unexplained death with postmortem examination. 展开更多
关键词 Forensic SCIENCES aconitine TOXIC effects METABOLIC CHARACTERISTICS
原文传递
Rapid construction of the unique BCD ring system of tricyclo[6.2.1.0]undecane in the C_(19)-diterpenoid alkaloid aconitine 被引量:1
15
作者 Xue Yang Bin Cheng +2 位作者 Hang Cheng Liang Xu Jian-Li Wang 《Chinese Chemical Letters》 SCIE CAS CSCD 2017年第8期1788-1792,共5页
A model study leading to the preparation of the unique tricyclo [6.2.1.0] undecane BCD ring systems of aconitine is described. The synthesis features an unprecedented diastereoselective oxidative dearomatization/dimer... A model study leading to the preparation of the unique tricyclo [6.2.1.0] undecane BCD ring systems of aconitine is described. The synthesis features an unprecedented diastereoselective oxidative dearomatization/dimerization/retro-DA/IMDA cascade reaction and a highly efficient Wagner-Meerwein rearrangement. 展开更多
关键词 aconitine Diterpenoid alkaloid Diels-Alder reaction Cascade reaction Wagner-Meerwein rearrangement
原文传递
Observation on Effects of Aconitine via Acupoint Injection in Rabbits
16
作者 倪峰 林静瑜 +4 位作者 郭丹 周春权 姚欣 叶蕻芝 吴广文 《Chinese Journal of Integrative Medicine》 SCIE CAS 2013年第1期36-41,共6页
Objective: To investigate the ability of the pericardium meridian (PM) to mitigate or enhance the cardiotoxic effects of aconitine injected at specific acupoint and non-acupoint sites in rabbits. Methods: This stu... Objective: To investigate the ability of the pericardium meridian (PM) to mitigate or enhance the cardiotoxic effects of aconitine injected at specific acupoint and non-acupoint sites in rabbits. Methods: This study consisted of 3 experiments that were designed to test the effects of injection of 30 IJg/kg of aconitine at acupoints on the PM (Test 1), at non-acupoint sites on the PM (Test 2), and at acupoints on other meridians and non-meridian sites (Test 3). in Test 1, 24 rabbits were randomly assigned to receive injections at Quze (PC3), Tianquan (PC2), or intramuscularly. In Test 2, 24 rabbits were randomly assigned to receive injections of aconitine at non-acupoint |, non-acupoint 11, or intramuscularly. In Test 3, 48 rabbits were randomly assigned to receive injections at Neiguan (PC6), Sanyinjiao (SP6), Yangjiao (GB35), a non-meridian and non-acupoint site (NMNA), intravenously, and intramuscularly. Electrocardiographs of the rabbits were performed before, during and after injection to determine the incidence of arrhythmia, latency of ventricular rhythm, and recovery rate after aconitine injection. The recovery time index and extent of arrhythmia scores were calculated. Results: In all groups the incidence of arrhythmia was 100%, and the latency of ventricular rhythm was less than 30 min. In Tests 1 and 2, the recovery rates of the Quze and non-acupoint I1 groups were significantly higher than those of the muscular group (P〈0.05). In Test 3, the recovery time index and extent of arrhythmia scores of the Neiguan group were low. There were no significant differences between the other acupoint groups, or the NMNA group, when compared with the group receiving aconitine intramuscularly. Conclusions: Acupoints or non-acupoints along the PM could reduce the severity of the arrhythmia induced by aconitine in healthy rabbits. Meridians play an important role in Drotecting bodv functions. 展开更多
关键词 aconitine ACUPOINT MERIDIAN acupuncture ARRHYTHMIA Chinese medicine
原文传递
Ginsenoside Rg_(1)Reduces Cardiotoxicity While Increases Cardiotonic Effect of Aconitine in vitro
17
作者 XU Xin XIE Xiao-fang +2 位作者 DONG Yan-hong ZHANG Hui-qiong PENG Cheng 《Chinese Journal of Integrative Medicine》 SCIE CAS CSCD 2022年第8期693-701,共9页
Objective To explore the synergic mechanism of ginsenoside Rg_(1)(Rg_(1))and aconitine(AC)by acting on normal neonatal rat cardiomyocytes(NRCMs)and pentobarbital sodium(PS)-induced damaged NRCMs.Methods The toxic,non-... Objective To explore the synergic mechanism of ginsenoside Rg_(1)(Rg_(1))and aconitine(AC)by acting on normal neonatal rat cardiomyocytes(NRCMs)and pentobarbital sodium(PS)-induced damaged NRCMs.Methods The toxic,non-toxic,and effective doses of AC and the most suitable compatibility concentration of Rg_(1) for both normal and damaged NRCMs exposed for 1 h were filtered out by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide,respectively.Then,normal NRCMs or impaired NRCMs were treated with chosen concentrations of AC alone or in combination with Rg_(1) for 1 h,and the cellular activity,cellular ultrastructure,apoptosis,leakage of acid phosphatase(ACP)and lactate dehydrogenase(LDH),intracellular sodium ions[Na^(+)],potassium ions[K^(+)]and calcium ions[Ca^(2+)]levels,and Nav1.5,Kv4.2,and RyR_(2) genes expressions in each group were examined.Results For normal NRCMs,3000µmol/L AC significantly inhibited cell viability(P<0.01),promoted cell apoptosis,and damaged cell structures(P<0.05),while other doses of AC lower than 3000µmol/L and the combinations of AC and Rg_(1) had little toxicity on NRCMs.Compared with AC acting on NRCMs alone,the co-treatment of 3000 and 10µmol/L AC with 1µmol/L Rg_(1) significantly decreased the level of intracellular Ca^(2+)(P<0.01 or P<0.05),and the co-treatment of 3000µmol/L AC with 1µmol/L Rg_(1) significantly decreased the level of intracellular Ca^(2+)via regulating Nav1.5,RyR_(2) expression(P<0.01).For damaged NRCMs,1500µmol/L AC aggravated cell damage(P<0.01),and 0.1 and 0.001µmol/L AC showed moderate protective effect.Compared with AC used alone,the co-treatment of Rg_(1) with AC reduced the cell damage,0.1µmol/L AC with 1µmol/L Rg_(1) significantly inhibited the level of intracellular Na+(P<0.05),1500µmol/L AC with 1µmol/L Rg_(1) significantly inhibited the level of intracellular K+(P<0.01)via regulating Nav1.5,Kv4.2,RyR_(2) expressions in impaired NRCMs.Conclusion Rg_(1) inhibited the cardiotoxicity and enhanced the cardiotonic effect of AC via regulating the ion channels pathway of[Na^(+)],[K^(+)],and[Ca^(2+)]. 展开更多
关键词 aconitine ginsenoside Rg_(1) compatibility ion channel pathway CARDIOMYOCYTES
原文传递
超高效液相色谱串联四极杆质谱联用分析动物血样中乌头类生物碱 被引量:5
18
作者 杜振霞 孙姝琦 《分析测试学报》 CAS CSCD 北大核心 2007年第z1期61-63,共3页
An ultra performance liquid chromatography-tandem mass spectrum method has been developed for the determination of three aconitum alkaloids (aconitine,hypaconitine and mesaconitine).The three alkaloids were analyzed s... An ultra performance liquid chromatography-tandem mass spectrum method has been developed for the determination of three aconitum alkaloids (aconitine,hypaconitine and mesaconitine).The three alkaloids were analyzed simultaneously with an Waters Acquity BHE C18 column by gradient elution using 0.05% aqueous ammonia-acetonitrile as mobile phase and detected by the MRM mode of the mass spectrum.The recoveries of the SPE method were between 68.79%-95.65% (RSD<7.94%,n=4),and all the alkaloids showed good linearity (r>0.998) in a relatively wide concentration range.The LOD reached 0.0516,0.0640,0.0744 ng/mL of aconitine,hypaconitine and mesaconitine,respectively.The analysis time was within 3 min which could meet the high-throughput detection.The results indicated that contents of alkaloids in animal blood can be well detected;and this method can be used in quality control of aconitum drugs and pharmacokinetics study. 展开更多
关键词 aconitine HYPaconitine MESaconitine Animal blood Ultra performance liquid chromatography-tandem mass spectrum
下载PDF
液相色谱-质谱联用测定乌头碱血药浓度及其药代动力学参数的方法学研究 被引量:12
19
作者 王朝虹 文蛟 何毅 《分析测试学报》 CAS CSCD 北大核心 2004年第z1期51-53,56,共4页
  乌头碱(aconitine)是存在于乌头属多种植物中的一种双酯二萜生物碱,具有镇[1]、抗炎[2]、抗癫痫[3]、抗肿瘤[4]、提高免疫功能[5]等多种生理活性,但因其具有强烈的心脏毒性和神经毒性,安全系数小,治疗剂量与中毒剂量接近(小鼠LD501....   乌头碱(aconitine)是存在于乌头属多种植物中的一种双酯二萜生物碱,具有镇[1]、抗炎[2]、抗癫痫[3]、抗肿瘤[4]、提高免疫功能[5]等多种生理活性,但因其具有强烈的心脏毒性和神经毒性,安全系数小,治疗剂量与中毒剂量接近(小鼠LD501.8mg/kg,op;人绝对致死量为2~5mg)[7],限制了对该化合物作为药用的进一步研究,目前仅将其作为致心率失常模型的工具药使用,尚未见其药代动力学的报道.在本文中首次应用液相色谱-质谱联用法对乌头碱在大鼠体内的药代动力学进行了研究.…… 展开更多
关键词 LC - MS Solid phase extraction aconitine PHARMACOKINETICS
下载PDF
乌头碱的LC-MS定量分析 被引量:2
20
作者 王俊伟 郭亚飞 +1 位作者 陈丽涛 王绘军 《分析测试学报》 CAS CSCD 北大核心 2004年第z1期57-61,共2页
  乌头生物碱各成分毒性差异很大,其中乌头碱的毒性为其它成分的100~2000倍,是引起中毒和死亡的主要原因.乌头生物碱种类多,在煎煮或泡制过程中易水解产生不同水解产物,进入体内后代谢情况又不明,因此采用液相色谱方法对体内检材乌...   乌头生物碱各成分毒性差异很大,其中乌头碱的毒性为其它成分的100~2000倍,是引起中毒和死亡的主要原因.乌头生物碱种类多,在煎煮或泡制过程中易水解产生不同水解产物,进入体内后代谢情况又不明,因此采用液相色谱方法对体内检材乌头碱成分仅靠保留时间确定依据不足,定量工作更是无法开展.…… 展开更多
关键词 aconitine Human blood QUANTITATIVE LC-MS
下载PDF
上一页 1 2 下一页 到第
使用帮助 返回顶部