期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
A Study on the Molecular Switch of Gene Expression of the Mouse Heart Nuclear DNA Fragments
1
作者 袁明秀 JIN +6 位作者 Rui Niu Weiran Chen Zhijun Zhang Zhihong 《High Technology Letters》 EI CAS 2001年第4期1-7,共7页
It is observed by in situ stain that LDH (1 5) ...nNAD + can probably enter the nucleopore and can be bound bound specifically with the genes that encode them. During the in vitro expression, the dilution of heart nuc... It is observed by in situ stain that LDH (1 5) ...nNAD + can probably enter the nucleopore and can be bound bound specifically with the genes that encode them. During the in vitro expression, the dilution of heart nuclear DNA fragments could enhance the expression activity of LDH/DNA and the amount of expressed LDH (1 5) is in proportion to the amount of dissociable LDH (1 5) on the LDH/DNA. With the integration of 14C Leu to the proteins, it is also observed that the addition of LDH (1 5) ...nNAD + can suppress the in vitro expression activity of LDH/DNA. AFM observation shows that the regulation sequence at the both ends of active genes may be bound with such active factors as proteins encoded by the genes which probably is the main molecular switch of gene expression and regulation we have been always searching for. Our work shows the prospective application of the combination of AFM and isotope labeling in the research of biological reaction. 展开更多
关键词 Molecular switch of gene expression Positive and negative feedback regulation mechanism gene expression and regulation AFM
下载PDF
Involvement of transcriptional enhancers in the regulation of developmental expression of yellow gene
2
作者 陈吉龙 刘洁 +3 位作者 陈智伟 张玉廉 郭树红 金振华 《Science China(Life Sciences)》 SCIE CAS 2001年第2期170-177,共8页
Upstream regulatory region and flanking DMA of yellow gene were isolated and cloned from a Drosophila genomic library. A vector containing yellow gene and regulatory elements was constructed using the recombinant DNA ... Upstream regulatory region and flanking DMA of yellow gene were isolated and cloned from a Drosophila genomic library. A vector containing yellow gene and regulatory elements was constructed using the recombinant DNA technique. Then this vector was integrated into Drosophila genome by genetic transformation. Using both FLP/FRT and Cre/LoxP site-specific recombination systems, two new yellow alleles were created at the same position in the genome of transgenic flies. Results from genetic and molecular analysis indicated that transcriptional enhancers regulate the developmental expression of the transgene. Furthermore, interactions between new-created yellow alleles were observed. Such interactions can influence markedly the expression of yellow gene during development. This effect may also be a form of enhancer-mediated gene expression. 展开更多
关键词 transcriptional enhancer TRANSgene gene expression and regulation Drosophila.
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部