In addition to causing discomfort, female mosquitoes introduce disease-carrying viruses and bacteria into the bloodstream of their victims. There are numerous publications describing the uses of sugary mosquito baits ...In addition to causing discomfort, female mosquitoes introduce disease-carrying viruses and bacteria into the bloodstream of their victims. There are numerous publications describing the uses of sugary mosquito baits with promising results. Without temperature control measures however, these methods are mainly useful for only nectar-feeding insects, including male mosquitoes, because the warmth of the blood is a condition for the females to locate their meals. The efforts required to keep the baits fresh against the natural spoiling process make them less attractive or impractical to implement. These experiments address these issues by using warm baits of water, sugar, boric acid, and antibiotics. Overnight, the general areas became clear of blood-sucking female mosquitoes while in numbers, the harmless males concentrated into the immediate vicinities. Control vs. experiment protocol established no other logical explanation for this phenomenon other than that females were attracted and killed by the bait. As expected, there was no female mosquito’s activity in these areas. There weren’t many left to do the work.展开更多
Background: In lacustrine communities, whether in Benin or elsewhere, populations use impregnated mosquito nets (IMNs) as fishing nets. This depletes the lake of its fishery resources, which in turn are contaminated b...Background: In lacustrine communities, whether in Benin or elsewhere, populations use impregnated mosquito nets (IMNs) as fishing nets. This depletes the lake of its fishery resources, which in turn are contaminated by the pyrethroids impregnated in the nets. This study aims to determine the scale and factors associated with the use of pyrethroid-impregnated mosquito nets as fishing tools in the municipality of Sô-Ava in Benin. Methods: This is a cross-sectional and analytical study with two components. First, a quantitative component was made up of 280 volunteers who were interviewed in the seven districts of the city. Data collection was done in two phases: the first from September to October 2020, and the second in August 2022. Data were analyzed with Stata and logistic regression was used. Another qualitative component was made up of forty participants chosen by reasoned choice, of which 32 were split into four focus groups of eight members each, and the rest participated in semi-structured interviews. Triangulation of the different sources was used to analyze the data. Results: Around 67% of the population reported using impregnated mosquito nets as fishing tools and 33% exclusively for malaria. Seasonal fishermen (ORa = 2.03, CI = 1.35 - 4.97, P = 0.004) and years of professional experience (ORa = 1.53, CI = 1.00 - 2.05, P = 0.021) increase the risk of using these nets as fishing tools. The use of insecticide-treated mosquito nets against mosquitoes causes skin scratching and impairs breathing because of the impregnation products, as reported by respondents. Conclusion: A high prevalence of inappropriate use of impregnated mosquito nets in fishing practices is highlighted in this study. Interviews in the field revealed that nets are diverted for fishing purposes to increase the volume of catches. Consequently, it will be appropriate to assess the pyrethroid content in water and fishery products.展开更多
The periodic outbreak of mosquito-borne diseases like dengue fever,zika fever,and yellow fever all over the world highlights the need for effective mosquito control methods targeting the biological system.Due to the l...The periodic outbreak of mosquito-borne diseases like dengue fever,zika fever,and yellow fever all over the world highlights the need for effective mosquito control methods targeting the biological system.Due to the lack of therapeutic measures,preventive treatments or vaccines against pathogens,insecticide resistance eventually lead the research focus towards novel technological applications in mosquito management.Nanomaterials with ovicidal,larvicidal,adulticidal,and repellent properties for controlling mosquito vectors are under research.A literature search was carried out for advancements in nanomaterials,insecticides,and mosquito control in PubMed/MEDLINE,Scopus,Google Scholar,ScienceDirect,and Web of Science.This paper aims to provide insights into various nanomaterials relevant to mosquito-borne diseases,in vivo and in vitro toxicity evaluation against mosquito species,mode of action,effect on non-target organisms,and ecological risks.Organic and inorganic materials that provide controlled release,target delivery,less dosage,prolonged efficacy,a reduction in the use of organic solvents and emulsifiers,and minimum pollution to the environment have already been explored.Indeed,further research on the ecological risk and economic feasibility of nanomaterials in mosquitocidal applications should be done prior to commercialization.展开更多
In the context of mosquito control, a plethora of devices have been put on the market. The effectiveness of these devices is not always proven, but some have interesting principles that have the potential to be a good...In the context of mosquito control, a plethora of devices have been put on the market. The effectiveness of these devices is not always proven, but some have interesting principles that have the potential to be a good means of mosquito control. Among these interesting devices are the photonic traps. We have carried out nightly captures of mosquitoes on human baits in the presence and absence of the device. These captures were made during the rainy season in the localities of Zogbadjè and Mènontin. The captures were made under the same atmospheric conditions at the same locations at 5-day intervals. These captures were made twice per house, indoors and outdoors, with one blank capture without the device and one capture with the device. The captured mosquitoes were identified and classified by genus and their aggressiveness determined by calculating the biting rate. A total of 845 mosquitoes were captured during these captures with 296 mosquitoes captured indoors without the device and 132 mosquitoes with the device present. This represents a decrease of 55.40% in culicidae density. Furthermore, a significant decrease was observed in aggressiveness, from 49 to 22 bites per man per night (b/m/n) with the use of the device. This decrease is even more important for nocturnal species such as Anopheles spp. whose aggressiveness decreased by 90%. The present study confirms the effectiveness of the “Electron go out mosquito small lamp” in reducing Culicidae density and mosquito aggression even in the presence of chemotactic interference. However, this study was limited in time and focused only on the ability of the device to reduce mosquito numbers and did not assess its epidemiological efficacy. It is therefore important to extend the work to examine the influence of the use of this device on population health and the occurrence of mosquito-borne diseases, particularly malaria.展开更多
Objective:To evaluate the entomological impact of chlorpyrifos-methyl,Bacillus thuringiensis, and Gambusia affinis on mosquitoes control in artificial breeding places.Methods:A Latin square design with 4 replicates wa...Objective:To evaluate the entomological impact of chlorpyrifos-methyl,Bacillus thuringiensis, and Gambusia affinis on mosquitoes control in artificial breeding places.Methods:A Latin square design with 4 replicates was performed in order to evaluate the efficacy of chlorpyrifosmethyl, Bacillus thuringiensis,and Gambusia affinis on larva.The larvicide was applied at the dosage of 100 mg a.h/ha,Bacillus thuringiensis at the recommended dosage and 10 fishes per m^2 were applied at 1×1 m^2 artificial breeding sites.The larval densities for both anopheline and culicine were counted according to larvae /10 dippers prior and 24 h after application.Results: All three control agents are effective for mosquito density reduction,and the difference between the three agents and the control is significant(P【0.05).There is also significant difference among chlorpyrifos-methyl,Bacillus thuringiensis and Gambusia affinis.Bacillus thuringiensis exhibited more reduction on mosquito larval density than fish and larvicide(P【0.05).Conclusions: Bacillus thuringiensis in comparison with two other agents is the appropriate method for larviciding in the breeding places.Although long term assessing for biological activities as well as monitoring and mapping of resistance is required.展开更多
Objective:Rice fields are dynamic mosquito larval habitats with assemblage of different predator taxa,including the larva of the mosquitoes Lutzia.Entomological surveillance in the ricefields is essential to evaluate ...Objective:Rice fields are dynamic mosquito larval habitats with assemblage of different predator taxa,including the larva of the mosquitoes Lutzia.Entomological surveillance in the ricefields is essential to evaluate the potential of these predators as biological resource to regulate vector mosquito population.In view of this,a survey of ricefields for immatures of different mosquito species including Lutzia was conducted.Methods:Survey of selected ricefields was carried out to evaluate the species composition of mosquitoes.Laboratory evaluation of the immatures of Lutzia mosquitoes was carried out to assess its predation potential using mosquitoes and chironomid as preys.Results:The survey revealed the presence of five mosquito species belonging to the genera Anopheles and Culex and the predatory immatures of the mosquito Lutzia Juscana(Wiedemann,1820).The ratio of prey and predatory larva ranged between 1.46 and 4.78 during the study period,with a significant correlation on the relative abundance of the larval stages of Lt.Juscana and Anopheles and Culex larvae.Under laboratory conditions,a single IV instar larvae of Lt.fuscana was found to consume on an average 5 to 15 equivalent instars of Anopheles sp.and Culex sp.larvae per day depending on its age.The prey consumption reduced with the larval stage approaching pupation.When provided with equal numbers of chironomid and Anopheles or Culex larvae,larva of Lt.fuscana consumed mosquito larvae significantly more compared to chironomids.Conclusion: The survey results and the preliminary study on predation are suggestive of the role of Lt.Juscana in the regulation of vector mosquito populations naturally in the ricefields.Since Lt.Juscana is common in many Asian countries,further studies on bioecology will be helpful to justify their use in mosquito control programme.展开更多
Reduction of mosquito populations will, at least, reduce substantially the transmission of malaria disease. One potential method of achieving this reduction is the environmentally-friendly population control method kn...Reduction of mosquito populations will, at least, reduce substantially the transmission of malaria disease. One potential method of achieving this reduction is the environmentally-friendly population control method known as the Sterile Insect Control (SIT) method. The SIT method has so far not been widely used against insect disease vectors, such as mosquitoes, because of various practical difficulties in rearing, sterilization and distribution of the parasite population. For mosquitoes, male-only release is considered essential since sterile females will bite and so may transmit disease, whereas male mosquitoes do not bite. This work concerns the mathematical modelling of the effectiveness of Sterile Insect Technique for Aedes aegypti mosquitoes, when the female sexual preference is incorporated. We found that for a released value of the sterile male mosquito below 40,000, the wild mosquito population decreases over time while the sterile male mosquito population increases. Therefore, the transmission of malaria and dengue infection declines because the sterile male mosquitoes dominated the environment. We also found that for a released value of the sterile male mosquito above 40,000, the wild mosquito population decreases and the sterile male mosquito population decreases as well. Therefore, if the injection of sterile male mosquitoes is large enough, the environment will be rid of mosquitoes over time. The result also shows that if sexual selection is incorporated into a reaction diffusion system, modelling the spread of Aedes aegypti mosquitoes, the Sterile Insect Technique (SIT) will still be a successful control measure.展开更多
Dear Editor The mosquitoes Aedes aegypli(I,) and Aedes albopictus (Skuse)(Diptera:Culicidae) are important vectors of dengue fever in tropical and subtropical parts of the world. Chemical control has been considered a...Dear Editor The mosquitoes Aedes aegypli(I,) and Aedes albopictus (Skuse)(Diptera:Culicidae) are important vectors of dengue fever in tropical and subtropical parts of the world. Chemical control has been considered as an effective tool for their management in different areas of the world.However, injudicious use of the chemicals result in environmental pollution,ill effects to humans and other animals,and the development of insecticide resistance,which ultimately limits the efficacy of many insecticides!’].Essential oils can be used for mosquito control with no harmful effects to the ecosystem and non-target organisms.Essentials oils from different plants have been found very展开更多
Objective:To determine resistance status of Culex species to different class of insecticides and assess the major anti-mosquito strategies employed by urban households and their possible effects on malaria acquisition...Objective:To determine resistance status of Culex species to different class of insecticides and assess the major anti-mosquito strategies employed by urban households and their possible effects on malaria acquisition.Methods:Structured questionnaires were randomly administered to obtain information on demographic characteristics,measures that people use to prevent mosquito bites and their perception of where mosquitoes breed in their communities.Adult susceptibility assays were also carried out using WHO discriminating dosages of four insecticides from different chemical classes against Culex species.Results:Majority of respondents(54.75%) preferred the use of domestic insecticides in the form of aerosols and coils.Among households that used domestic insecticides,the use of coil was most frequent(62.9%) with a mean(95%CI) of 9.18(8.99,9.37) coils per week.Strong level of pyrethroid-resistance and multiple insecticide resistance in Culex species were also detected in some of the study sites.Conclusions:The excessive use of domestic insecticides and high level of resistance in Culex species observed in the study area has implications for the ITN component of the nation's malaria control program in more subtle ways.People will lose interest in the use of ITN when it fails to protect users from bites of resistant Culex species.Excessive use of domestic insecticides may also select resistance in both malaria vectors and Culex species.On this account we recommend that nuisance mosquitoes must be controlled as part of malaria control programs to improve acceptance and utilization of ITN.展开更多
Dear editor,Mosquito-borne diseases,viz.,malaria,filariasis,dengue hemorrhagic fever,chikungunya,Japanese encephalitis etc.,created huge impact on humans over the world,and the chemical insecticides remain the mainsta...Dear editor,Mosquito-borne diseases,viz.,malaria,filariasis,dengue hemorrhagic fever,chikungunya,Japanese encephalitis etc.,created huge impact on humans over the world,and the chemical insecticides remain the mainstay of effective control.But,indiscriminate and rampant use of the chemical insecticides in controlling mosquito vectors,in order to prevent diseases vectored by them,has resulted problems related to the adverse environmental effects for their(insecticides)potential toxicity,high operational cost,community acceptance,and the development of insecticide resistance among the vectors.The factors mentioned above prompted the search for new means of control strategies.Currently,the botanicals(viz.,plant extracts,essential oils and phytochemicals)with mosquitocidal potential展开更多
Here, we have synthesized the silver nanoparticles (AgNPs) by using the soil fungus Aspergillus niger 2587. The results recorded from UV-vis spectrophotometer and transmission electron microscopy (TEM) support the bio...Here, we have synthesized the silver nanoparticles (AgNPs) by using the soil fungus Aspergillus niger 2587. The results recorded from UV-vis spectrophotometer and transmission electron microscopy (TEM) support the biosynthesis and characterization of AgNPs. The synthesized silver nanoparticles have also been tested against the larvae and pupae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti. The efficacy test was performed at different concentrations for a period of different hours by the probit analysis. The larvae of Cx. quinquefasciatus have shown the 100% mortality to the synthesized AgNPs after 1 h of exposure, while the larvae of An. stephensi and Ae. aegypti were found less susceptible to the synthesized AgNPs. The pupa of Ae. aegypti has shown the efficacy LC50 4, LC90 12 and LC99 19 ppm after 2 h of exposure of the synthesized AgNPs, while, the pupae of Cx. quinquefasciatus and An. stephensi were found less susceptible to the synthesized AgNPs. By this approach, it is suggestive that this rapid synthesis of nanoparticles would be proper for developing a biological process for mosquito control.展开更多
Objective:To determine the current insecticide resistance status of Aedes(Ae.)aegypti and Ae.albopictus to four insecticides,namely 0.05%deltamethrin,0.75%permethrin,5%malathion and 0.25%pirimiphos-methyl using the Wo...Objective:To determine the current insecticide resistance status of Aedes(Ae.)aegypti and Ae.albopictus to four insecticides,namely 0.05%deltamethrin,0.75%permethrin,5%malathion and 0.25%pirimiphos-methyl using the World Health Organisation(WHO)susceptibility test kit.Methods:Adult bioassays were carried out using the standard protocol of the World Health Organisation.All F1 generation urban and suburban field strains of Ae.aegypti and Ae.albopictus were tested against pyrethroid and organophosphate insecticides,including the presence of piperonyl butoxide(PBO)in four replicates of 25 non-blood-fed female mosquitoes ranging from 3 to 5 days old.The Vector Control Research Unit(VCRU)laboratory strain served as a reference strain.Results:In this study,0.05%deltamethrin demonstrated a lower value of knockdown time when 50%of the mosquito population died(KT50)and knockdown time when 95%of the mosquito population died(KT95),which is significantly more effective compared to 0.75%permethrin against adult female Ae.aegypti(urban and suburban)and Ae.albopictus(urban and suburban)(ANOVA,P<0.01).Meanwhile,5%malathion was a more effective insecticide,amounting to the shorter KT50 and KT95 compared to 0.25%pirimiphos-methyl against Ae.aegypti(urban and suburban)and Ae.albopictus(urban and suburban).Ae.aegypti urban and Ae.aegypti suburban performed a higher resistance ratio(RR)towards both 0.05%deltamethrin and 0.75%permethrin due to the wide use of permethrin in dengue vector control programs in Malaysia.However,Ae.albopictus urban and suburban have lower resistance than Ae.aegypti urban and suburban towards 0.05%deltamethrin and 0.75%permethrin at 24 hours post-treatment.The addition of PBO with these insecticides successfully reduced knockdown time(KT50 and KT95)values of most of the Ae.aegypti and Ae.albopictus field strains except PBO+0.75%permethrin against Ae.aegypti suburban.Conclusions:The addition of PBO to insecticides has significantly reduced the knockdown time(KT50 and KT95)values on most of Ae.aegypti and Ae.albopictus urban strain except PBO+5%malathion against Ae.albopictus urban strain and PBO+0.75%permethrin against Ae.albopictus suburban strain in comparison to exposure to insecticides without PBO.Ae.aegypti showed a higher resistance ratio of 50(RR50)when compared with the VCRU laboratory reference strain(susceptible strain)at the exposure to the deltamethrin,including with pre-exposure to PBO.This study found that the addition of PBO with organophosphates(5%malathion and 0.25%pirimiphos-methyl)was significantly more effective than pyrethroids against Ae.aegypti and Ae.albopictus(urban and suburban)due to their high mortality rate at 24 hours.It can be concluded that the usage of PBO can help reduce resistance alteration in Aedes mosquitoes.展开更多
Background Mosquito research in Europe has a long history,primarily focused on malaria vectors.In recent years,invasive mosquito species like the Asian tiger mosquito(Aedes albopictus)and the spread of arboviruses lik...Background Mosquito research in Europe has a long history,primarily focused on malaria vectors.In recent years,invasive mosquito species like the Asian tiger mosquito(Aedes albopictus)and the spread of arboviruses like dengue virus,chikungunya virus or bluetongue virus have led to an intensifcation of research and monitoring in Europe.The risk of further dissemination of exotic species and mosquito-borne pathogens is expected to increase with ongoing globalization,human mobility,transport geography,and climate warming.Researchers have conducted various studies to understand the ecology,biology,and efective control strategies of mosquitoes and associated pathogens.Main body Three invasive mosquito species are established in Europe:Asian tiger mosquito(Aedes albopictus),Japanese bush mosquito(Ae.japonicus),and Korean bush mosquito(Aedes koreicus).Ae.albopictus is the most invasive species and has been established in Europe since 1990.Over the past two decades,there has been an increasing number of outbreaks of infections by mosquito-borne viruses in particular chikungunya virus,dengue virus or Zika virus in Europe primary driven by Ae.albopictus.At the same time,climate change with rising temperatures results in increasing threat of invasive mosquito-borne viruses,in particular Usutu virus and West Nile virus transmitted by native Culex mosquito species.Efective mosquito control programs require a high level of community participation,going along with comprehensive information campaigns,to ensure source reduction and successful control.Control strategies for container breeding mosquitoes like Ae.albopictus or Culex species involve community participation,door-to-door control activities in private areas.Further measures can involve integration of sterile insect techniques,applying indigenous copepods,Wolbachia sp.bacteria,or genetically modifed mosquitoes,which is very unlike to be practiced as standard method in the near future.Conclusions Climate change and globalization resulting in the increased establishment of invasive mosquitoes in particular of the Asian tiger mosquito Ae.albopictus in Europe within the last 30 years and increasing outbreaks of infections by mosquito-borne viruses warrants intensifcation of research and monitoring.Further,efective future mosquito control programs require increase in intense community and private participation,applying physical,chemical,biological,and genetical control activities.展开更多
文摘In addition to causing discomfort, female mosquitoes introduce disease-carrying viruses and bacteria into the bloodstream of their victims. There are numerous publications describing the uses of sugary mosquito baits with promising results. Without temperature control measures however, these methods are mainly useful for only nectar-feeding insects, including male mosquitoes, because the warmth of the blood is a condition for the females to locate their meals. The efforts required to keep the baits fresh against the natural spoiling process make them less attractive or impractical to implement. These experiments address these issues by using warm baits of water, sugar, boric acid, and antibiotics. Overnight, the general areas became clear of blood-sucking female mosquitoes while in numbers, the harmless males concentrated into the immediate vicinities. Control vs. experiment protocol established no other logical explanation for this phenomenon other than that females were attracted and killed by the bait. As expected, there was no female mosquito’s activity in these areas. There weren’t many left to do the work.
文摘Background: In lacustrine communities, whether in Benin or elsewhere, populations use impregnated mosquito nets (IMNs) as fishing nets. This depletes the lake of its fishery resources, which in turn are contaminated by the pyrethroids impregnated in the nets. This study aims to determine the scale and factors associated with the use of pyrethroid-impregnated mosquito nets as fishing tools in the municipality of Sô-Ava in Benin. Methods: This is a cross-sectional and analytical study with two components. First, a quantitative component was made up of 280 volunteers who were interviewed in the seven districts of the city. Data collection was done in two phases: the first from September to October 2020, and the second in August 2022. Data were analyzed with Stata and logistic regression was used. Another qualitative component was made up of forty participants chosen by reasoned choice, of which 32 were split into four focus groups of eight members each, and the rest participated in semi-structured interviews. Triangulation of the different sources was used to analyze the data. Results: Around 67% of the population reported using impregnated mosquito nets as fishing tools and 33% exclusively for malaria. Seasonal fishermen (ORa = 2.03, CI = 1.35 - 4.97, P = 0.004) and years of professional experience (ORa = 1.53, CI = 1.00 - 2.05, P = 0.021) increase the risk of using these nets as fishing tools. The use of insecticide-treated mosquito nets against mosquitoes causes skin scratching and impairs breathing because of the impregnation products, as reported by respondents. Conclusion: A high prevalence of inappropriate use of impregnated mosquito nets in fishing practices is highlighted in this study. Interviews in the field revealed that nets are diverted for fishing purposes to increase the volume of catches. Consequently, it will be appropriate to assess the pyrethroid content in water and fishery products.
文摘The periodic outbreak of mosquito-borne diseases like dengue fever,zika fever,and yellow fever all over the world highlights the need for effective mosquito control methods targeting the biological system.Due to the lack of therapeutic measures,preventive treatments or vaccines against pathogens,insecticide resistance eventually lead the research focus towards novel technological applications in mosquito management.Nanomaterials with ovicidal,larvicidal,adulticidal,and repellent properties for controlling mosquito vectors are under research.A literature search was carried out for advancements in nanomaterials,insecticides,and mosquito control in PubMed/MEDLINE,Scopus,Google Scholar,ScienceDirect,and Web of Science.This paper aims to provide insights into various nanomaterials relevant to mosquito-borne diseases,in vivo and in vitro toxicity evaluation against mosquito species,mode of action,effect on non-target organisms,and ecological risks.Organic and inorganic materials that provide controlled release,target delivery,less dosage,prolonged efficacy,a reduction in the use of organic solvents and emulsifiers,and minimum pollution to the environment have already been explored.Indeed,further research on the ecological risk and economic feasibility of nanomaterials in mosquitocidal applications should be done prior to commercialization.
文摘In the context of mosquito control, a plethora of devices have been put on the market. The effectiveness of these devices is not always proven, but some have interesting principles that have the potential to be a good means of mosquito control. Among these interesting devices are the photonic traps. We have carried out nightly captures of mosquitoes on human baits in the presence and absence of the device. These captures were made during the rainy season in the localities of Zogbadjè and Mènontin. The captures were made under the same atmospheric conditions at the same locations at 5-day intervals. These captures were made twice per house, indoors and outdoors, with one blank capture without the device and one capture with the device. The captured mosquitoes were identified and classified by genus and their aggressiveness determined by calculating the biting rate. A total of 845 mosquitoes were captured during these captures with 296 mosquitoes captured indoors without the device and 132 mosquitoes with the device present. This represents a decrease of 55.40% in culicidae density. Furthermore, a significant decrease was observed in aggressiveness, from 49 to 22 bites per man per night (b/m/n) with the use of the device. This decrease is even more important for nocturnal species such as Anopheles spp. whose aggressiveness decreased by 90%. The present study confirms the effectiveness of the “Electron go out mosquito small lamp” in reducing Culicidae density and mosquito aggression even in the presence of chemotactic interference. However, this study was limited in time and focused only on the ability of the device to reduce mosquito numbers and did not assess its epidemiological efficacy. It is therefore important to extend the work to examine the influence of the use of this device on population health and the occurrence of mosquito-borne diseases, particularly malaria.
文摘Objective:To evaluate the entomological impact of chlorpyrifos-methyl,Bacillus thuringiensis, and Gambusia affinis on mosquitoes control in artificial breeding places.Methods:A Latin square design with 4 replicates was performed in order to evaluate the efficacy of chlorpyrifosmethyl, Bacillus thuringiensis,and Gambusia affinis on larva.The larvicide was applied at the dosage of 100 mg a.h/ha,Bacillus thuringiensis at the recommended dosage and 10 fishes per m^2 were applied at 1×1 m^2 artificial breeding sites.The larval densities for both anopheline and culicine were counted according to larvae /10 dippers prior and 24 h after application.Results: All three control agents are effective for mosquito density reduction,and the difference between the three agents and the control is significant(P【0.05).There is also significant difference among chlorpyrifos-methyl,Bacillus thuringiensis and Gambusia affinis.Bacillus thuringiensis exhibited more reduction on mosquito larval density than fish and larvicide(P【0.05).Conclusions: Bacillus thuringiensis in comparison with two other agents is the appropriate method for larviciding in the breeding places.Although long term assessing for biological activities as well as monitoring and mapping of resistance is required.
文摘Objective:Rice fields are dynamic mosquito larval habitats with assemblage of different predator taxa,including the larva of the mosquitoes Lutzia.Entomological surveillance in the ricefields is essential to evaluate the potential of these predators as biological resource to regulate vector mosquito population.In view of this,a survey of ricefields for immatures of different mosquito species including Lutzia was conducted.Methods:Survey of selected ricefields was carried out to evaluate the species composition of mosquitoes.Laboratory evaluation of the immatures of Lutzia mosquitoes was carried out to assess its predation potential using mosquitoes and chironomid as preys.Results:The survey revealed the presence of five mosquito species belonging to the genera Anopheles and Culex and the predatory immatures of the mosquito Lutzia Juscana(Wiedemann,1820).The ratio of prey and predatory larva ranged between 1.46 and 4.78 during the study period,with a significant correlation on the relative abundance of the larval stages of Lt.Juscana and Anopheles and Culex larvae.Under laboratory conditions,a single IV instar larvae of Lt.fuscana was found to consume on an average 5 to 15 equivalent instars of Anopheles sp.and Culex sp.larvae per day depending on its age.The prey consumption reduced with the larval stage approaching pupation.When provided with equal numbers of chironomid and Anopheles or Culex larvae,larva of Lt.fuscana consumed mosquito larvae significantly more compared to chironomids.Conclusion: The survey results and the preliminary study on predation are suggestive of the role of Lt.Juscana in the regulation of vector mosquito populations naturally in the ricefields.Since Lt.Juscana is common in many Asian countries,further studies on bioecology will be helpful to justify their use in mosquito control programme.
文摘Reduction of mosquito populations will, at least, reduce substantially the transmission of malaria disease. One potential method of achieving this reduction is the environmentally-friendly population control method known as the Sterile Insect Control (SIT) method. The SIT method has so far not been widely used against insect disease vectors, such as mosquitoes, because of various practical difficulties in rearing, sterilization and distribution of the parasite population. For mosquitoes, male-only release is considered essential since sterile females will bite and so may transmit disease, whereas male mosquitoes do not bite. This work concerns the mathematical modelling of the effectiveness of Sterile Insect Technique for Aedes aegypti mosquitoes, when the female sexual preference is incorporated. We found that for a released value of the sterile male mosquito below 40,000, the wild mosquito population decreases over time while the sterile male mosquito population increases. Therefore, the transmission of malaria and dengue infection declines because the sterile male mosquitoes dominated the environment. We also found that for a released value of the sterile male mosquito above 40,000, the wild mosquito population decreases and the sterile male mosquito population decreases as well. Therefore, if the injection of sterile male mosquitoes is large enough, the environment will be rid of mosquitoes over time. The result also shows that if sexual selection is incorporated into a reaction diffusion system, modelling the spread of Aedes aegypti mosquitoes, the Sterile Insect Technique (SIT) will still be a successful control measure.
文摘Dear Editor The mosquitoes Aedes aegypli(I,) and Aedes albopictus (Skuse)(Diptera:Culicidae) are important vectors of dengue fever in tropical and subtropical parts of the world. Chemical control has been considered as an effective tool for their management in different areas of the world.However, injudicious use of the chemicals result in environmental pollution,ill effects to humans and other animals,and the development of insecticide resistance,which ultimately limits the efficacy of many insecticides!’].Essential oils can be used for mosquito control with no harmful effects to the ecosystem and non-target organisms.Essentials oils from different plants have been found very
文摘Objective:To determine resistance status of Culex species to different class of insecticides and assess the major anti-mosquito strategies employed by urban households and their possible effects on malaria acquisition.Methods:Structured questionnaires were randomly administered to obtain information on demographic characteristics,measures that people use to prevent mosquito bites and their perception of where mosquitoes breed in their communities.Adult susceptibility assays were also carried out using WHO discriminating dosages of four insecticides from different chemical classes against Culex species.Results:Majority of respondents(54.75%) preferred the use of domestic insecticides in the form of aerosols and coils.Among households that used domestic insecticides,the use of coil was most frequent(62.9%) with a mean(95%CI) of 9.18(8.99,9.37) coils per week.Strong level of pyrethroid-resistance and multiple insecticide resistance in Culex species were also detected in some of the study sites.Conclusions:The excessive use of domestic insecticides and high level of resistance in Culex species observed in the study area has implications for the ITN component of the nation's malaria control program in more subtle ways.People will lose interest in the use of ITN when it fails to protect users from bites of resistant Culex species.Excessive use of domestic insecticides may also select resistance in both malaria vectors and Culex species.On this account we recommend that nuisance mosquitoes must be controlled as part of malaria control programs to improve acceptance and utilization of ITN.
文摘Dear editor,Mosquito-borne diseases,viz.,malaria,filariasis,dengue hemorrhagic fever,chikungunya,Japanese encephalitis etc.,created huge impact on humans over the world,and the chemical insecticides remain the mainstay of effective control.But,indiscriminate and rampant use of the chemical insecticides in controlling mosquito vectors,in order to prevent diseases vectored by them,has resulted problems related to the adverse environmental effects for their(insecticides)potential toxicity,high operational cost,community acceptance,and the development of insecticide resistance among the vectors.The factors mentioned above prompted the search for new means of control strategies.Currently,the botanicals(viz.,plant extracts,essential oils and phytochemicals)with mosquitocidal potential
文摘Here, we have synthesized the silver nanoparticles (AgNPs) by using the soil fungus Aspergillus niger 2587. The results recorded from UV-vis spectrophotometer and transmission electron microscopy (TEM) support the biosynthesis and characterization of AgNPs. The synthesized silver nanoparticles have also been tested against the larvae and pupae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti. The efficacy test was performed at different concentrations for a period of different hours by the probit analysis. The larvae of Cx. quinquefasciatus have shown the 100% mortality to the synthesized AgNPs after 1 h of exposure, while the larvae of An. stephensi and Ae. aegypti were found less susceptible to the synthesized AgNPs. The pupa of Ae. aegypti has shown the efficacy LC50 4, LC90 12 and LC99 19 ppm after 2 h of exposure of the synthesized AgNPs, while, the pupae of Cx. quinquefasciatus and An. stephensi were found less susceptible to the synthesized AgNPs. By this approach, it is suggestive that this rapid synthesis of nanoparticles would be proper for developing a biological process for mosquito control.
基金the Fundamental Research Grant Scheme,Ministry of Higher Education Malaysia(FRGS/1/2023/STG03/USM/02/4).
文摘Objective:To determine the current insecticide resistance status of Aedes(Ae.)aegypti and Ae.albopictus to four insecticides,namely 0.05%deltamethrin,0.75%permethrin,5%malathion and 0.25%pirimiphos-methyl using the World Health Organisation(WHO)susceptibility test kit.Methods:Adult bioassays were carried out using the standard protocol of the World Health Organisation.All F1 generation urban and suburban field strains of Ae.aegypti and Ae.albopictus were tested against pyrethroid and organophosphate insecticides,including the presence of piperonyl butoxide(PBO)in four replicates of 25 non-blood-fed female mosquitoes ranging from 3 to 5 days old.The Vector Control Research Unit(VCRU)laboratory strain served as a reference strain.Results:In this study,0.05%deltamethrin demonstrated a lower value of knockdown time when 50%of the mosquito population died(KT50)and knockdown time when 95%of the mosquito population died(KT95),which is significantly more effective compared to 0.75%permethrin against adult female Ae.aegypti(urban and suburban)and Ae.albopictus(urban and suburban)(ANOVA,P<0.01).Meanwhile,5%malathion was a more effective insecticide,amounting to the shorter KT50 and KT95 compared to 0.25%pirimiphos-methyl against Ae.aegypti(urban and suburban)and Ae.albopictus(urban and suburban).Ae.aegypti urban and Ae.aegypti suburban performed a higher resistance ratio(RR)towards both 0.05%deltamethrin and 0.75%permethrin due to the wide use of permethrin in dengue vector control programs in Malaysia.However,Ae.albopictus urban and suburban have lower resistance than Ae.aegypti urban and suburban towards 0.05%deltamethrin and 0.75%permethrin at 24 hours post-treatment.The addition of PBO with these insecticides successfully reduced knockdown time(KT50 and KT95)values of most of the Ae.aegypti and Ae.albopictus field strains except PBO+0.75%permethrin against Ae.aegypti suburban.Conclusions:The addition of PBO to insecticides has significantly reduced the knockdown time(KT50 and KT95)values on most of Ae.aegypti and Ae.albopictus urban strain except PBO+5%malathion against Ae.albopictus urban strain and PBO+0.75%permethrin against Ae.albopictus suburban strain in comparison to exposure to insecticides without PBO.Ae.aegypti showed a higher resistance ratio of 50(RR50)when compared with the VCRU laboratory reference strain(susceptible strain)at the exposure to the deltamethrin,including with pre-exposure to PBO.This study found that the addition of PBO with organophosphates(5%malathion and 0.25%pirimiphos-methyl)was significantly more effective than pyrethroids against Ae.aegypti and Ae.albopictus(urban and suburban)due to their high mortality rate at 24 hours.It can be concluded that the usage of PBO can help reduce resistance alteration in Aedes mosquitoes.
基金funded by the Federal Ministry of Education and Research of Germany(BMBF)under the project NEED(Grant no.01Kl2022).
文摘Background Mosquito research in Europe has a long history,primarily focused on malaria vectors.In recent years,invasive mosquito species like the Asian tiger mosquito(Aedes albopictus)and the spread of arboviruses like dengue virus,chikungunya virus or bluetongue virus have led to an intensifcation of research and monitoring in Europe.The risk of further dissemination of exotic species and mosquito-borne pathogens is expected to increase with ongoing globalization,human mobility,transport geography,and climate warming.Researchers have conducted various studies to understand the ecology,biology,and efective control strategies of mosquitoes and associated pathogens.Main body Three invasive mosquito species are established in Europe:Asian tiger mosquito(Aedes albopictus),Japanese bush mosquito(Ae.japonicus),and Korean bush mosquito(Aedes koreicus).Ae.albopictus is the most invasive species and has been established in Europe since 1990.Over the past two decades,there has been an increasing number of outbreaks of infections by mosquito-borne viruses in particular chikungunya virus,dengue virus or Zika virus in Europe primary driven by Ae.albopictus.At the same time,climate change with rising temperatures results in increasing threat of invasive mosquito-borne viruses,in particular Usutu virus and West Nile virus transmitted by native Culex mosquito species.Efective mosquito control programs require a high level of community participation,going along with comprehensive information campaigns,to ensure source reduction and successful control.Control strategies for container breeding mosquitoes like Ae.albopictus or Culex species involve community participation,door-to-door control activities in private areas.Further measures can involve integration of sterile insect techniques,applying indigenous copepods,Wolbachia sp.bacteria,or genetically modifed mosquitoes,which is very unlike to be practiced as standard method in the near future.Conclusions Climate change and globalization resulting in the increased establishment of invasive mosquitoes in particular of the Asian tiger mosquito Ae.albopictus in Europe within the last 30 years and increasing outbreaks of infections by mosquito-borne viruses warrants intensifcation of research and monitoring.Further,efective future mosquito control programs require increase in intense community and private participation,applying physical,chemical,biological,and genetical control activities.