Reversed-Phase-HPLC Assay Method for Simultaneous Estimation of Sorbitol, Sodium Lactate, and Sodium Chlorides in Pharmaceutical Formulations and Drug Solution for Infusion

A rapid, straightforward, sensitive, efficient, and cost-effective reverse-phase high-performance liquid chromatographic method was employed for the simultaneous determination of Sorbitol, Sodium Lactate, and Chlorides in a drug solution for infusion. Sorbitol, Sodium lactate, and Chloride are all officially recognized in the USP monograph. Assay methods are provided through various techniques, with titrations being ineffective for trace-level quantification. Alternatively, IC, AAS, and ICP-MS, though highly accurate, are costly and often unavailable to most testing facilities. When considering methods, it’s important to prioritize both quality control requirements and user-friendly techniques. A simple HPLC simultaneous method was developed for the quantification of Chlorides, Sorbitol, and Sodium Lactate with a shorter run time. The separation utilized a Shimpack SCR-102(H) ion exclusion analytical column (7.9 mm × 300 mm, 7 μm), with a flow rate of 0.6 mL per min. The column compartment temperature was maintained at 40°C, and the injection volume was set at 10 μL, with detection at 200 nm. All measurements were conducted in a 0.1% solution of phosphoric acid. The analytical curves demonstrated linearity (r > 0.9999) in the concentration range of 0.79 to 3.8 mg per mL for Sodium Lactate (SL), 0.16 to 0.79 mg per mL for Sodium Chloride (SC), and 1.5 to 7.2 mg per mL for Sorbitol. Validation of the developed method followed the guidelines of the International Conference on Harmonization (ICH Q2B) and USP. The method exhibited precision, robustness, accuracy, and selectivity. In accelerated stability testing over 6 months, no significant variations were observed in organoleptic analysis and pH. Consequently, the developed method is deemed suitable for routine quality control analyses, enabling the simultaneous determination of Sodium Lactate, Sodium Chloride, and Sorbitol in pharmaceutical formulations and infusions.

Effectiveness of Sodium Silicate on the Corrosion Protection of AA7075-T6 Aluminium Alloy in Sodium Chloride Solution

The influence of sodium silicate on the corrosion behaviour of aluminium alloy 7075-T6 in 0.1 M sodium chloride solution was studied by open circuit potential (OCP) and electrochemical impedance spectroscopy (EIS) techniques. Scanning electron microscopy (SEM) was used to characterize the AA7075-T6 surface. Silicate can significantly reduce corrosion deterioration and the inhibition efficiency increases with the concentration of Na2SiO3. The corrosion inhibition mechanism involves the formation of a protective film over the alloy surface by adsorption of aluminosilicate anions from solution, as has also been suggested by others in literature.

Whole-process optimization for industrial production of glucosamine sulfate sodium chloride based on QbD concept

The double salt of glucosamine sulfate sodium chloride(glucosamine-SP) is an important pharmaceuticals ingredient for healing osteoarthritis. However, the study about its industrial production is rarely documented, let alone the optimization over the whole process to produce glucosamine-SP using glucosamine hydrochloride and anhydrous sodium sulfate as synthetic raw materials. In order to improve the production efficiency, this study screened the process parameters based on the concept of quality by design(QbD), optimized 13 operational parameters related to reaction and separation in the process, and finally proposed the mixed dropping process. The reaction conditions for the preparation of glucosamineSP were found as follows: the molar ratio of anhydrous sodium sulfate to glucosamine hydrochloride is 0.42, the mass ratio of water to glucosamine hydrochloride is is 2.0, the reaction temperature is 50 ℃ and the reaction time is 1 h. Through step-by-step scaling up following QbD, the mixed dropping process was successfully applied to achieve a trial production of 200 kg products satisfying national quality standards.In all, the results of this study have high technical value and guiding significance for the industrial mass production of glucosamine-SP.

Changes of protein oxidation,lipid oxidation and lipolysis in Chinese dry sausage with different sodium chloride curing salt content

The effect of sodium chloride(NaCl)curing salt content on protein oxidation,lipid oxidation and lipolysis of Chinese dry sausage was investigated.Two groups Chinese dry sausages with 2%and 4%(m/m)salt content were studied.The degree of protein oxidation increased during the processes in two groups sausages,while the content of phospholipids decreased,neutral lipids and free fatty acids increased.The degree of protein oxidation,lipid oxidation and lipolysis in 4%NaCl content group was higher than those in 2%NaCl content group,while 4%NaCl content group has higher lipase activity.In conclusion,4%NaCl may facilitate the protein oxidation,lipid hydrolysis and oxidation in Chinese dry sausage,and the protein oxidation had strong correlation with lipid oxidation and lipolysis.The results could provide a basis for improving the technology of industrial production.

Manifestations in Corrosion Prophase of Ultra-high Strength Steel 30CrMnSiNi2 A in Sodium Chloride Solutions

The corrosion behaviors of ultra-high strength steel 30CrMnSiNi2A in sodium chloride solution were studied by weight loss and electrochemical methods. The morphology of corrosion products was observed using scanning electron microscopy（SEM） and the composition was analyzed using an energy dispersive spectroscopy（EDS） and X-Ray diffraction （XRD）. The experimental results showed that the corrosion came from pitting corrosion and the rust layer was composed of outer rust layer γ-FeOOH and inner rust layer Fe_2O_3 with a little β-FeOOH. The correlation between corrosion rate and test time accorded with exponential rule. The corrosion current measured by polarization methods was higher than that calculated by weight loss method after a long-time immersion, the main reason was that,β-FeOOH and γ-Fe_2O_3 transformed by γ-FeOOH led to overestimating corrosion rate. The processes of corrosion prophase were obtained from XRD and EIS results. The corrosion product, Fe（OH）_2 formed at the initial stage stayed at a non-steady state and then consequently transferred to γ-FeOOH, γ-Fe_2O_3 or β-FeOOH.

Enhanced extraction of scandium and inhibiting of iron from acid leaching solution of red mud by D2EHPA and sodium chloride

D2EHPA(P204),tri-butyl-phosphate(TBP)and sodium chloride(NaCl)were attractive for selective extraction of scandium from acid leaching solution of red mud.The extraction parameters of P204 concentration(X_(P204)),NaCl concentration(C_(NaCl)),pH value,reaction time,stirring speed and O/A were investigated to extract scandium and separate iron from the acid leaching solution.The extraction mechanism was analyzed by Fourier transform infrared spectroscopy(FT-IR)and thermodynamic theory.The single-stage extraction efficiency of scandium,iron andβ(Sc/Fe)were 99.1%,9.4%and 1061.2,respectively,with C_(NaCl) of 75 g/L and XP204 of 0.75 at solution pH value of 1.2 and stirring speed of 200 r/min for 6 min,in which a good separation effect of scandium and iron was obtained.The vibration absorption peak Sc─O was contributed to the extraction of scandium with P204.The complex[FeCln]^(3−n) existed in the solution with adding NaCl into the acid leaching solution.The value of n was higher and the valence state of the complex[FeCln]^(3−n) was lower with an increase of chloride concentration,which restricts the extraction efficiency of iron with P204.The extraction of three stages in the counter-current simulation experiments was carried out according to the McCabe-Thiele diagram.Gibbs free energy change(ΔG)of−5.93 kJ/mol,enthalpy change(ΔH)of 23.45 kJ/mol and entropy change(ΔH)of 98.54 J/(mol·K)were obtained in the solvent extraction proces,which indicate that the extraction reaction is easily spontaneous and endothermic and a proper increase of temperature is conducive to the extraction of scandium.

Effect of Sodium Chloride and Cadmium on the Growth, Oxidative Stress and Antioxidant Enzyme Activities of Zygosaccharomyces rouxii

Zygosaccharomyces rouxii is a salt-tolerant yeast species capable of removing cadmium(Cd) pollutant from aqueous solution. Presently, the physiological characteristics of Z. rouxii under the stress of sodium chloride(NaCl) and Cd are poorly understood. This study investigated the effects of NaCl and Cd on the growth, oxidative stress and antioxidant enzyme activities of Z. rouxii after stress treatment for 24 h. Results showed that NaCl or Cd alone negatively affected the growth of Z. rouxii, but the growth-inhibiting effect of Cd on Z. rouxii was reduced in the presence of NaCl. Flow cytometry assay showed that under Cd stress, NaCl significantly reduced the production of reactive oxygen species(ROS) and cell death of Z. rouxii compared with those in the absence of NaCl. The activities of superoxide dismutase(SOD), catalase(CAT), and peroxidase(POD) of Z. rouxii were significantly enhanced by 2%–6% NaCl, which likely contributed to the high salt tolerance of Z. rouxii. The POD activity was inhibited by 20 mg L-1Cd while the SOD and CAT activities were enhanced by 8 mg L-1 Cd and inhibited by 20 mg L-1 or 50 mg L-1 Cd. The inhibitory effect of high-level Cd on the antioxidant enzyme activities of Z. rouxii was counteracted by the combined use of NaCl, especially at 6%. This probably accounted for the decrease in Cd-induced ROS production and cell death of Z. rouxii after incubation with NaCl and Cd. Our work provided physiological clues as to the use of Z. rouxii as a biosorbent for Cd removal from seawater and liquid highly salty food.

Effect of Sodium Chloride on Meltability of Mozzarella Cheese

Meltability is one of the most important properties of Mozzarella cheese, as it is generally used in pizza and other foods. Mozzarella was prepared by no salted and immature production technology, and the effect of different addition amounts of salt on the meltability of mozzarella cheese was measured by Schreiber method and small amplitude oscillatory shear analysis（SAOSA） method. The results showed that different adding amounts of NaCl had significant influence on the meltability of Mozzarella cheese, and 2% NaCl addition was the best condition. The results measured by the methods of Schreiber and SAOSA were basically same： adding different amounts of NaCl had significant influence on the hardness and elasticity of mozzarella cheese, but no significant influence on the sticky. It was a good microscopic structure arrangement of Mozzarella cheese with 2% NaCl addition. Scanning electron micrographs showed that a space grid structure formed by casein had changed, and formed many uniform molecular holes. The results indicated that different addition amounts of salt had influence on meltability of no salted immature Mozzarella cheese, and this technology could be drastically shorten the processing time.

Generation of sodium hypochlorite (NaOCl) from sodium chloride solution using C/PbO_2 and Pb/PbO_2 electrodes

Two modified electrodes （Pb/PbO2 and C/PbO2） were prepared by electrodepositing a lead oxide layer on lead and carbon sub- strates. These modified electrodes were used as anodes for the generation of sodium hypochlorite （NaOC1） from sodium chloride solution. Different operating conditions and factors affecting the treatment process of NaOC1 generation, including current density, pH values, con- ductive electrolytes, and electrolysis time, were studied and optimized. By comparison the C/PbO2 electrode shows a higher efficiency than the Pb/PbO2 electrode for the generation of NaOC1.

Corrosion Resistance ofAA6063-Type AI-Mg-Si Alloy by Silicon Carbide in Sodium Chloride Solution for Marine Application

The present work focused on corrosion inhibition of AA6063 type Al-Mg-Si alloy in sodium chloride （NaCI） solution with a silicon carbide inhibitor, using the potentiodynamic electrochemical method. The aluminium alloy surface morphology was examined, in the as-received and as-corroded in the un-inhibited state, with scanning electron microscopy equipped with energy dispersive spectroscopy （SEM-EDS）. The results obtained via linear polarization indicated a high corrosion potential for the unprotected as-received alloy. Equally, inhibition efficiency as high as 98.82% at 10.0 g/v silicon carbide addition was obtained with increased polarization resistance fRy）, while the current density reduced significantly for inhibited samples compared to the un-inhibited aluminium alloy. The adsorption mechanism of the inhibitor aluminium alloy follows the Langmuir adsorption isotherm. This shows that the corrosion rate of aluminium alloy with silicon carbide in NaCI environment decreased significantly with addition of the inhibitor.

Corrosion Fatigue Behavior of Duplex Stainless Steel in 3.5% Sodium Chloride Solution

The corrosion fatigue behavior of duplex stainless steel (DSS) was studied at different cyclic stress levels in 3.5%NaCl (mass fraction, so as the follows) solution (pH=7) at 50 degreesC. The results showed that DSS was susceptible to pitting corrosion and corrosion fatigue. Both intergranluar corrosion cracking and transgranlular corrosion cracking initiated at the bottom of pitting holes. Furthermore, the corrosion fatigue properties of DSS in 3.5%NaCl solution may be relatived to complex electrochemical and mechanical coupling effects between the three phases (austenite, ferrite and martensite), where martensite and ferrite were anodic in the corrosion cell and could be prone to cracking under certain condition.

Sodium chloride methanol solution spin-coating process for bulk-heterojunction polymer solar cells

The sodium chloride methanol solution process is conducted on the conventional poly（3-hexylthiophene）（P3HT）/[6,6]-phenyl-C61-butyric acid methyl ester（PC_（61）BM） polymer bulk heterojunction solar cells. The device exhibits a power conversion efficiency of up to 3.36%, 18% higher than that of the device without the solution process. The measurements of the active layer by x-ray photoelectron spectroscopy（XPS）, atomic force microscopy（AFM）, and ultraviolet photoelectron spectroscopy（UPS） indicate a slight phase separation in the vertical direction and a sodium chloride distributed island-like interface between the active layer and the cathode. The capacitance–voltage（C–V） and impedance spectroscopy measurements prove that the sodium chloride methanol process can reduce the electron injection barrier and improve the interfacial contact of polymer solar cells. Therefore, this one-step solution process not only optimizes the phase separation in the active layers but also forms a cathode buffer layer, which can enhance the generation, transport, and collection of photogenerated charge carriers in the device simultaneously. This work indicates that the inexpensive and non-toxic sodium chloride methanol solution process is an efficient one-step method for the low cost manufacturing of polymer solar cells.

Three-dimensional oxygen-doped porous graphene:Sodium chloridetemplate preparation,structural characterization and supercapacitor performances

Supercapacitor is a new type of energy storage device,which has the advantages of high-power property and long cycle life.In this study,three-dimensional graphene(3 D-GN)with oxygen doping and porous structure was prepared from graphene oxide(GO)by an inexpensive sodium chloride(NaCl)template,as a promising electrode material for the supercapacitor.The structure,morphology,specific surface area,pore size,of the sample were characterized by XRD,SEM,TEM and BET techniques.The electrochemical performances of the sample were tested by CV and CDC techniques.The 3 D-GE product is a threedimensional nano material with hierarchical porous structures,its specific surface area is much larger than that of routine stacked graphene(GN),and it contains a large number of mesoporous and macropores,a small amount of micropores.The capacitance characteristics of the 3 D-GN electrode material are excellent,showing high specific capacitance(173.5 F·g^(-1)at 1 A·g^(-1)),good rate performance(109.2 F·g^(-1)at 8 A·g^(-1))and long cycle life(88%capacitance retention after 10,000 cycles at 8 A·g^(-1))

Study on the Diffusion Coefficient of Sodium Chloride at Infinite Dilution in Supercritical Water

The molecular dynamics (MD) was employed to simulate the diffusion coefficient of sodium chloride at infinite dilution in supercritical water from 703.2 K to 763.2 K and from 30 MPa to 45 MPa. Based on the simulated data and the Patel-Teja(PT) equation of state and the Liu-Ruckenstein equation, an equation for calculating the diffusion coefficient of NaCl at infinite dilution in supercritical water is proposed. Both the agreement between the simulated and correlated data, and that between the simulated and predicted data of diffusion coefficients for NaCl in supercritical water ranging from 703.2K to 803.2 K and from 25 MPa to 50 MPa show that this equation is applicable for the calculation of diffusion coefficients.

Effect of Different Amounts of Sodium Chloride on the Meltability of Mozzarella Cheese

The effect of different amounts of salt（NaCl）on the meltability of Mozzarella cheese was measured by Schreiber method and small amplitude oscillatory shear analysis（SAOSA）method.The results showed that different addition amounts of NaCl had significant influence on the meltability of Mozzarella cheese,and2%NaCl addition was the best condition for cheese melting.The results measured by Schreiber method and SAOSA method were basically the same.Adding different amounts of NaCl had significant influence on the hardness and elasticity of Mozzarella cheese but no significant influence on the stickiness.The microscopic structure of Mozzarella cheese with 2%NaCl addition was arranged orderly.Scanning electron micrographs showed the space grid structure formed by casein had changed and formed many uniform molecular holes.

The Study on the Corrosion Behaviour of Welded and Unwelded Medium Carbon Steel in Sodium Chloride(Nacl)Solutions

The research work was based on the study of the corrosion behaviour of the welded and un-welded medium carbon steel in sodium chloride solutions.The Sodium chloride solutions used are 1ml,2ml,3ml and 4ml for both welded and un-welded medium carbon steel in NaCl.The experiments were conducted in two ways,the weight loss analyses of measurements and using the electrochemical analyzer workstation to determine the potential dynamic of the samples.The samples for the weight loss measurements were prepared from rolled products obtained at the foundry shop.Two medium carbon steel materials were sourced with different chemical compositions as sample A and B.The materials were prepared to accommodate the experiments for the determination of welded and un-welded medium carbon steel.A total of sixty-eight(68)samples were produced,prepared and used for the weight loss measurements/analyses the experiments.Thirty-four of the samples each were prepared for both the welded and un-welded experiments.All the samples were produced and prepared through the use of various machining processes with the use of a lathe machine for planning,milling.Thirty-four(34)of the sample preparation were further welded in readiness of the experiments.Sixty-eight breakers were sourced for and used.Ten(10)other samples were used for the determination with the use of the electrochemical analyzer.The chemical compositions of the medium carbon steel were determined with the use of SPECTRO Analytical Instruments.A metallurgical inverted optical microscope was used to determine the microstructures of the materials.The Scanning Electron Microscopy with EDS was used to determine the morphologies of the materials.The thirty-four of the samples were welded this process was performed to determine the effects of welding on the material surrounding the weldments.These materials were made into sizes with the use of power hacksaw(i.e.2cm by 2cm).Other materials were prepared to 1cm x 1cm thickness from the same materials.The Tafel plot experiments and that of the open Circuit Potential Time(OCPT)were carried out with the use of Electrochemical Analyzer/Workstation.The Medium carbon steel materials were exposed for fifty-four(54)days,with an interval of 3days.The corrosion rates analyses were determined and the graphs of the corrosion rates(mm/yr.)and other parameters were used plotted against No of days exposed.

Novel Reduction of Arylsulfonyl Chlorides and Sodium Alkyl Thiosulfates to Disulfides with TiCl_4/Zn System

T1Cl4/Zn system reduces arylsulfonyl chlorides and sedium aldyl thiosulfates to corresponding disulfides in moderate to good yields

Evaluation of the Efficacy and Safety of 1%Sodium Chloride(LiceFreee Spray)against 1%Permethrin Creme Rinse on Head Lice Infested Individuals

Head lice are a public health issue, and resistance to available over-the-counter pediculicides is a concern. The objective of this randomized study was to evaluate the pediculicidal activity and safety of 1% Sodium Chloride spray (0.1709 M), (LiceFreee Spray&#174) compared to the current recommended treatment for head lice with 1% Permethrin Crème Rinse. Forty-two subjects were randomized equally into Sodium Chloride or Permethrin group. Products were applied to hair according to the label instructions. After application of the products at Day 1 and Day 8, pediculicidal efficacy and safety were assessed at Day 1, Day 8 and Day 15. Second treatment was only applied on Day 8 to individuals with observed live lice using the same products and protocols as Day 1. Proportion of lice free subjects per group, the reduction in number of live lice per head and adverse effects were recorded after each visit. The results showed significantly higher pediculicidal activity for Sodium Chloride spray (85%) as compared to Permethrin (45%) at Day 15 (p 0.05). Similar numbers of lice per head (21.76 range 10 to 68 versus 21.29 range 10 to 60 for Sodium Chloride and Permethrin groups, respectively) were observed for individuals at Day 1. At Day 15, lice per head infested reduced to 0.55 ± 1.50 in Sodium Chloride spray group compared to 5.45 ± 7.91 in the Permethrin group (p 0.01). No serious adverse effects were observed in both groups. Sodium Chloride spray had superior efficacy to 1% Permethrin Crème Rinse in treating head lice and is a safe and excellent alternative to the current recommended treatment.

Visualizing the crystallization of sodium chloride under supersaturated condition

Crystallization in supersaturated solution plays a fundamental role in a variety of natural and industrial processes.However,a thorough understanding of crystallization phenomena in supersaturated solution is still difficult because the real-time visualization of crystallization processes under supersaturated condition is a great challenge.Herein,an electron beam-induced crystallization method was carried out in in situ liquid cell transmission electron microscopy(TEM)to visualize the crystallization of NaCl under supersaturated condition in real time.Crucial steps and behaviors in the crystallization of NaCl were captured and clarified,including the growth of NaCl nanocrystals with different morphologies,the formation of initial crystalline seeds from amorphous ion clusters,and the non-equilibrium growth behaviors caused by uneven distribution of precursor ions.This study provides the real-time visualization of detailed nucleation and growth behaviors in NaCl crystallization and brings an ideal strategy for investigating crystallization phenomena under supersaturated condition.

The Effect of Calcium Chloride on the Microbiological Characteristics of Fermented Chile Pepper （Capsicum annuum cv. Mesilla Cayenne） Mash

Fermented chile pepper mash （Capsicum annuum cv. Mesilla Cayenne） is a major industrial food product in New Mexico. The fermentation of chile pepper mash depends on temperature, acidity, salt concentration, dissolved air, available carbohydrates and enzymes. The objective of this study was to evaluate the effects of the addition of calcium chloride （CaCI2） on the microbial characteristics of the pepper mash fermentation. Nine five gallon buckets were prepared with pepper that had been washed and ground by the manufacturer, each with 15% sodium chloride added. The buckets were allotted randomly to 1 of 3 treatments. The treatments included no CaCl2, 0.2% CaCl2 and 0.4% CaCl2 added to the pepper mash. Samples were stored at room temperature and sampled over a 3 months period to examine changes in the microflora. Chile mash samples were serially diluted in butterfield＇s phosphate buffer for microbial enumeration. Aerobic plate counts were conducted by pour plating with an overlay. Petrifilms were used for E. coil/coliform counts and Enterobacteriaceae. Yeasts and molds were surface plated on Rose Bengal Chloramphenicol agar while streptococci and lactobacilli were plated onto M17 and acidified MRS respectively. Aerobic plate counts, coliforms, E. coli, Enterobacteriaceae, yeast, mold, lactobacilli, and streptococci were not affected by CaCl2 level. The addition of 0.4% CaCl2 was found to have no effect on the chile pepper mash fermentation.