目的探讨血清抗Vinculin足细胞自身抗体在儿童特发性肾病综合征(INS)中的表达阳性率,及对INS诊断、预后的价值。方法采用ELISA方法检测80例INS患儿及320例健康儿童血清Vinculin-IgG抗体水平,根据抗体滴度水平将80例INS患儿分为抗体阳性...目的探讨血清抗Vinculin足细胞自身抗体在儿童特发性肾病综合征(INS)中的表达阳性率,及对INS诊断、预后的价值。方法采用ELISA方法检测80例INS患儿及320例健康儿童血清Vinculin-IgG抗体水平,根据抗体滴度水平将80例INS患儿分为抗体阳性组和阴性组,并对不同组病例的临床资料进行分析。结果与健康儿童相比较,INS患儿抗Vinculin抗体阳性率显著升高(30/80 vs 10/320,P<0.05),抗体阳性率与患儿激素敏感性无关(24/63 vs 6/13,P>0.05)。与抗体阴性INS患儿相比较,抗体阳性INS患儿24 h尿蛋白定量水平升高[(3200.7±1286.8)mg/24 h vs(1051.2±266.3)mg/24 h,P<0.05]。抗体阳性患儿组血清抗Vinculin抗体滴度与INS患儿24 h尿蛋白定量存在相关性(r=0.586,P<0.05),尿蛋白转阴后,抗体滴度较确诊时明显下降(P<0.05)。结论血清抗Vinculin足细胞抗体在INS早期诊断、疗效评估、预后判断中可能具有重要的临床意义。展开更多
Rho-associated protein kinase is an essential regulator of cytoskeletal dynamics during the process of neurite extension. However, whether Rho kinase regulates microtubule remodeling or the distri- bution of adhesive ...Rho-associated protein kinase is an essential regulator of cytoskeletal dynamics during the process of neurite extension. However, whether Rho kinase regulates microtubule remodeling or the distri- bution of adhesive proteins to mediate neurite outgrowth remains unclear. By specifically modulat- ing Rho kinase activity with pharmacological agents, we studied the morpho-dynamics of neurite outgrowth. We found that lysophosphatidic acid, an activator of Rho kinase, inhibited neurite out- growth, which could be reversed by Y-27632, an inhibitor of Rho kinase. Meanwhile, reorganization of microtubules was noticed during these processes, as indicated by their significant changes in the soma and growth cone. In addition, exposure to lysophosphatidic acid led to a decreased mem- brane distribution of vinculin, a focal adhesion protein in neurons, whereas Y-27632 recruited vin- culin to the membrane. Taken together, our data suggest that Rho kinase regulates rat hippocampal neurite growth and microtubule formation via a mechanism associated with the redistribution of vinculin.展开更多
目的应用同位素标记相对与绝对定量蛋白组学(isobaric tags for relative and absolute quantification,iTRAQ)技术鉴定和筛选进展期胃癌复发转移相关的差异蛋白;检测筛选出的目标蛋白Vinculin表达水平及其预后意义。方法应用iTRAQ技术...目的应用同位素标记相对与绝对定量蛋白组学(isobaric tags for relative and absolute quantification,iTRAQ)技术鉴定和筛选进展期胃癌复发转移相关的差异蛋白;检测筛选出的目标蛋白Vinculin表达水平及其预后意义。方法应用iTRAQ技术在正常胃黏膜组织组、胃癌复发组和胃癌未复发组,检测差异表达的蛋白。然后选取差异性表达显著的Vinculin蛋白,采用免疫组织化学(immunohistochemistry,IHC)技术在110例人胃癌组织标本中检测其表达情况,并统计分析Vinculin蛋白表达量与临床病理学指标的关系;采用Kaplan-Meier生存曲线分析,探究Vinculin蛋白表达与胃癌患者生存期的关系。结果Vinculin蛋白主要表达于细胞质中近胞膜处;在110例人胃癌组织标本中,Vinculin蛋白阳性表达46例(41.8%),缺失表达64例(58.2%)。统计结果显示,Vinculin蛋白缺失表达与淋巴结转移(P=0.018)、肿瘤TNM分期(P=0.022)、血管侵袭(P=0.002)显著正相关。Kaplan-Meier生存曲线分析结果显示,Vinculin缺失表达与胃癌患者不良预后正相关(P=0.011)。结论Vinculin蛋白缺失表达与肿瘤转移和预后不良相关,提示其表达可以作为预测胃癌侵袭转移的潜在指标。展开更多
In the present study,we aimed to globally profile the proteins involved in colorectal carcinoma（CRC）,in order to find clues to the pathological process of CRC.Pairs of malignant tissues and their adjacent healthy ti...In the present study,we aimed to globally profile the proteins involved in colorectal carcinoma（CRC）,in order to find clues to the pathological process of CRC.Pairs of malignant tissues and their adjacent healthy tissues from patients with colorectal cancer were subject to differential proteomics analysis.Two dimensional electrophoresis coupled with mass spectrometry（2-DE/MS） was used to identify differentially expressed proteins between pairs of tissue samples.A list of proteins relevant to the progression of colorectal tumor was identified by two dimensional gel electrophoresis（2-DE）-based proteomics approach.Among the identified proteins,vinculin was found to be remarkably down-regulated in colorectal carcinoma tissues.In addition,three phosphorylation modifications within the isolated vinculin were identified by in-depth liquid chromatography-tandem mass spectrometry（LC-MS/MS） analysis.Our results provide a basis for further understanding the pathological significance of vinculin in the regulation of carcinogenesis,invasion and metastasis of colorectal tumors.展开更多
Poly(ADP-ribose)(PAR)is a highly negatively charged polymer.PAR is synthesized by poly(ADP-ribose)polymerases(PARPs)and is involved in the assembly and stabilization of macromolecular complexes.Here,the presence and p...Poly(ADP-ribose)(PAR)is a highly negatively charged polymer.PAR is synthesized by poly(ADP-ribose)polymerases(PARPs)and is involved in the assembly and stabilization of macromolecular complexes.Here,the presence and putative roles of poly(ADP-ribosyl)ation(PARylation)associated to adherens junctions(AJ)and the actin cytoskeleton in epithelial and Schwann cells,is reviewed.The hypothesis generated by analogy,stating that PAR is associated to AJ in other cell types,is postulated.According to this hypothesis,PAR associated to puncta adherentia in chemical synapses would participate in plasticity,learning and memory.In turn,PAR associated to fascia adherens in cardiomyocytes,would affect heart beating.PARP inhibitors are currently under development and clinical testing.Basic research in different tissues will probably influence their clinical uses.展开更多
文摘目的探讨血清抗Vinculin足细胞自身抗体在儿童特发性肾病综合征(INS)中的表达阳性率,及对INS诊断、预后的价值。方法采用ELISA方法检测80例INS患儿及320例健康儿童血清Vinculin-IgG抗体水平,根据抗体滴度水平将80例INS患儿分为抗体阳性组和阴性组,并对不同组病例的临床资料进行分析。结果与健康儿童相比较,INS患儿抗Vinculin抗体阳性率显著升高(30/80 vs 10/320,P<0.05),抗体阳性率与患儿激素敏感性无关(24/63 vs 6/13,P>0.05)。与抗体阴性INS患儿相比较,抗体阳性INS患儿24 h尿蛋白定量水平升高[(3200.7±1286.8)mg/24 h vs(1051.2±266.3)mg/24 h,P<0.05]。抗体阳性患儿组血清抗Vinculin抗体滴度与INS患儿24 h尿蛋白定量存在相关性(r=0.586,P<0.05),尿蛋白转阴后,抗体滴度较确诊时明显下降(P<0.05)。结论血清抗Vinculin足细胞抗体在INS早期诊断、疗效评估、预后判断中可能具有重要的临床意义。
基金supported by the National Natural Science Foundation of China,No.31170941the Fundamental Research Funds for the Central Universities,No.21612424the Science and Technology Planning Project of Guangdong Province,No.2010B031600102
文摘Rho-associated protein kinase is an essential regulator of cytoskeletal dynamics during the process of neurite extension. However, whether Rho kinase regulates microtubule remodeling or the distri- bution of adhesive proteins to mediate neurite outgrowth remains unclear. By specifically modulat- ing Rho kinase activity with pharmacological agents, we studied the morpho-dynamics of neurite outgrowth. We found that lysophosphatidic acid, an activator of Rho kinase, inhibited neurite out- growth, which could be reversed by Y-27632, an inhibitor of Rho kinase. Meanwhile, reorganization of microtubules was noticed during these processes, as indicated by their significant changes in the soma and growth cone. In addition, exposure to lysophosphatidic acid led to a decreased mem- brane distribution of vinculin, a focal adhesion protein in neurons, whereas Y-27632 recruited vin- culin to the membrane. Taken together, our data suggest that Rho kinase regulates rat hippocampal neurite growth and microtubule formation via a mechanism associated with the redistribution of vinculin.
文摘目的应用同位素标记相对与绝对定量蛋白组学(isobaric tags for relative and absolute quantification,iTRAQ)技术鉴定和筛选进展期胃癌复发转移相关的差异蛋白;检测筛选出的目标蛋白Vinculin表达水平及其预后意义。方法应用iTRAQ技术在正常胃黏膜组织组、胃癌复发组和胃癌未复发组,检测差异表达的蛋白。然后选取差异性表达显著的Vinculin蛋白,采用免疫组织化学(immunohistochemistry,IHC)技术在110例人胃癌组织标本中检测其表达情况,并统计分析Vinculin蛋白表达量与临床病理学指标的关系;采用Kaplan-Meier生存曲线分析,探究Vinculin蛋白表达与胃癌患者生存期的关系。结果Vinculin蛋白主要表达于细胞质中近胞膜处;在110例人胃癌组织标本中,Vinculin蛋白阳性表达46例(41.8%),缺失表达64例(58.2%)。统计结果显示,Vinculin蛋白缺失表达与淋巴结转移(P=0.018)、肿瘤TNM分期(P=0.022)、血管侵袭(P=0.002)显著正相关。Kaplan-Meier生存曲线分析结果显示,Vinculin缺失表达与胃癌患者不良预后正相关(P=0.011)。结论Vinculin蛋白缺失表达与肿瘤转移和预后不良相关,提示其表达可以作为预测胃癌侵袭转移的潜在指标。
基金Supported by the National Natural Science Foundation of China(No.21107055)the Natural Science Foundation of Jilin Province,China(Nos.200705387,2011713,20090461,20110739)the Natural Science Foundation of Heilongjiang Province,China(Nos.201000742,D201075,D200873,GC09C317)
文摘In the present study,we aimed to globally profile the proteins involved in colorectal carcinoma（CRC）,in order to find clues to the pathological process of CRC.Pairs of malignant tissues and their adjacent healthy tissues from patients with colorectal cancer were subject to differential proteomics analysis.Two dimensional electrophoresis coupled with mass spectrometry（2-DE/MS） was used to identify differentially expressed proteins between pairs of tissue samples.A list of proteins relevant to the progression of colorectal tumor was identified by two dimensional gel electrophoresis（2-DE）-based proteomics approach.Among the identified proteins,vinculin was found to be remarkably down-regulated in colorectal carcinoma tissues.In addition,three phosphorylation modifications within the isolated vinculin were identified by in-depth liquid chromatography-tandem mass spectrometry（LC-MS/MS） analysis.Our results provide a basis for further understanding the pathological significance of vinculin in the regulation of carcinogenesis,invasion and metastasis of colorectal tumors.
文摘Poly(ADP-ribose)(PAR)is a highly negatively charged polymer.PAR is synthesized by poly(ADP-ribose)polymerases(PARPs)and is involved in the assembly and stabilization of macromolecular complexes.Here,the presence and putative roles of poly(ADP-ribosyl)ation(PARylation)associated to adherens junctions(AJ)and the actin cytoskeleton in epithelial and Schwann cells,is reviewed.The hypothesis generated by analogy,stating that PAR is associated to AJ in other cell types,is postulated.According to this hypothesis,PAR associated to puncta adherentia in chemical synapses would participate in plasticity,learning and memory.In turn,PAR associated to fascia adherens in cardiomyocytes,would affect heart beating.PARP inhibitors are currently under development and clinical testing.Basic research in different tissues will probably influence their clinical uses.
