Objective:To investigate the effect ofα1-antitrypsin combined with bone marrow mesenchymal stem cells on retinopathy in diabetic rats and its mechanism.Methods:A model of diabetic retinopathy was established by intra...Objective:To investigate the effect ofα1-antitrypsin combined with bone marrow mesenchymal stem cells on retinopathy in diabetic rats and its mechanism.Methods:A model of diabetic retinopathy was established by intraperitoneal injection of streptozotocin.The 30 Wistar rats successfully modeled were randomly divided into a model group,a bone marrow mesenchymal stem cell group and a combined group(α1-antitrypsin combined with bone marrow Mesenchymal stem cells),the blood glucose and serum insulin levels of diabetic rats were measured 4 weeks after treatment.Enzyme-linked immunosorbent assay(ELISA)for measuring serum inflammatory factors IL-1β,IL-6 and TNF-α in rats.Observing the pathological morphology of rat retina under hematoxylin-eosin staining(HE).TUNEL staining to observe the apoptosis of rat retinal nerve cells.Immunohistochemical method to detect the expression level of CD45 in retinal tissue.Real-time fluorescence quantitative PCR was used to detect the expression of retinal vascular endothelial growth factor(VEGF),hypoxiainducible factor-1α(HIF-1α),and angiotensinⅡ(ANGⅡ)mRNA.Western blot was used to detect the expression of p38 MAPK/NF-κB signaling pathway-related proteins in the retinal tissue of each group of rats.Results:Compared with the control group,the rats in the model group had increased blood glucose,decreased insulin levels,increased serum IL-1β,IL-6,and TNF-α levels,and had obvious lesions in the retina.CD45 showed high expression in retinal tissue,VEGF,HIF-1α,ANGⅡ mRNA expression increased,p-p38,p-p65,p-IκBα protein expression increased(P<0.05).Compared with the model group,the bone marrow mesenchymal stem cell group and the combined group have decreased blood glucose,increased insulin levels,and decreased serum IL-1β,IL-6 and TNF-α levels.Retinopathy is improved,apoptosis of retinal nerve cells is reduced,CD45 expression in retinal tissue is reduced,VEGF,HIF-1α,ANGⅡ mRNA expression is decreased,and p-p38,p-p65,p-IκBα protein expression is decreased.Compared with the bone marrow mesenchymal stem cell group,the effect of the combined group was more obvious(P<0.05).Conclusion:α1-antitrypsin combined with bone marrow mesenchymal stem cell transplantation can improve the degree of retinopathy in diabetic rats.The mechanism may be related to the inhibition of p38 MAPK/NF-κB signaling pathway.展开更多
Summary: By using immunohistochemistry LSAB method and imaging analysis technique, the ex- pression of al-antitrypsinase (α1-AT) in 41 cases of bronchioalveolar carcinoma (BAC) was quan- titatively detected to explo...Summary: By using immunohistochemistry LSAB method and imaging analysis technique, the ex- pression of al-antitrypsinase (α1-AT) in 41 cases of bronchioalveolar carcinoma (BAC) was quan- titatively detected to explore the relationship between α1-AT expression in BAC tissues and clinical pathology. The results showed that the total positive rate for α1-AT expression was 85. 4 %. The positive rate for α1-AT expression in alveolar BAC was 100 %, with the immunity reactive stain- ing intensity being significantly higher than in papillary BAC, mucinous BAC or sclerosing BAC (P<0. 05). The positive rate in papillary BAC was 93. 3 %, with the intensity higher mucinous BAC or sclerosing BAC (P<0. 01); The positive rate in both mucinous BAC and sclerosing BAC was 66. 7 % (P>0. 05); The expression intensity in lymph node metastatic group was obviously lower than that in the group without metastasis (P<0.01); The patients with mucinous BAC were diagnosed at a younger age than those with other histologic types of BAC (P<0. 05). It was suggested that BAC cells could also produce al-AT. Detection of α1-AT could be used as a new method to diagnose BAC and might play a role in assessing BAC metastasis.展开更多
基金Key Rearch and Development projects in Shaanxi Province(2019SF-084).
文摘Objective:To investigate the effect ofα1-antitrypsin combined with bone marrow mesenchymal stem cells on retinopathy in diabetic rats and its mechanism.Methods:A model of diabetic retinopathy was established by intraperitoneal injection of streptozotocin.The 30 Wistar rats successfully modeled were randomly divided into a model group,a bone marrow mesenchymal stem cell group and a combined group(α1-antitrypsin combined with bone marrow Mesenchymal stem cells),the blood glucose and serum insulin levels of diabetic rats were measured 4 weeks after treatment.Enzyme-linked immunosorbent assay(ELISA)for measuring serum inflammatory factors IL-1β,IL-6 and TNF-α in rats.Observing the pathological morphology of rat retina under hematoxylin-eosin staining(HE).TUNEL staining to observe the apoptosis of rat retinal nerve cells.Immunohistochemical method to detect the expression level of CD45 in retinal tissue.Real-time fluorescence quantitative PCR was used to detect the expression of retinal vascular endothelial growth factor(VEGF),hypoxiainducible factor-1α(HIF-1α),and angiotensinⅡ(ANGⅡ)mRNA.Western blot was used to detect the expression of p38 MAPK/NF-κB signaling pathway-related proteins in the retinal tissue of each group of rats.Results:Compared with the control group,the rats in the model group had increased blood glucose,decreased insulin levels,increased serum IL-1β,IL-6,and TNF-α levels,and had obvious lesions in the retina.CD45 showed high expression in retinal tissue,VEGF,HIF-1α,ANGⅡ mRNA expression increased,p-p38,p-p65,p-IκBα protein expression increased(P<0.05).Compared with the model group,the bone marrow mesenchymal stem cell group and the combined group have decreased blood glucose,increased insulin levels,and decreased serum IL-1β,IL-6 and TNF-α levels.Retinopathy is improved,apoptosis of retinal nerve cells is reduced,CD45 expression in retinal tissue is reduced,VEGF,HIF-1α,ANGⅡ mRNA expression is decreased,and p-p38,p-p65,p-IκBα protein expression is decreased.Compared with the bone marrow mesenchymal stem cell group,the effect of the combined group was more obvious(P<0.05).Conclusion:α1-antitrypsin combined with bone marrow mesenchymal stem cell transplantation can improve the degree of retinopathy in diabetic rats.The mechanism may be related to the inhibition of p38 MAPK/NF-κB signaling pathway.
文摘Summary: By using immunohistochemistry LSAB method and imaging analysis technique, the ex- pression of al-antitrypsinase (α1-AT) in 41 cases of bronchioalveolar carcinoma (BAC) was quan- titatively detected to explore the relationship between α1-AT expression in BAC tissues and clinical pathology. The results showed that the total positive rate for α1-AT expression was 85. 4 %. The positive rate for α1-AT expression in alveolar BAC was 100 %, with the immunity reactive stain- ing intensity being significantly higher than in papillary BAC, mucinous BAC or sclerosing BAC (P<0. 05). The positive rate in papillary BAC was 93. 3 %, with the intensity higher mucinous BAC or sclerosing BAC (P<0. 01); The positive rate in both mucinous BAC and sclerosing BAC was 66. 7 % (P>0. 05); The expression intensity in lymph node metastatic group was obviously lower than that in the group without metastasis (P<0.01); The patients with mucinous BAC were diagnosed at a younger age than those with other histologic types of BAC (P<0. 05). It was suggested that BAC cells could also produce al-AT. Detection of α1-AT could be used as a new method to diagnose BAC and might play a role in assessing BAC metastasis.
