Objective： To study the intervention effects of Jianpi Liqi Huoxue Decoction （健脾理气活血汤, JLHD） on lipid peroxidative liver injury induced by alcohol. Methods： The rat alcoholic model of liver disease （ALD） ...Objective： To study the intervention effects of Jianpi Liqi Huoxue Decoction （健脾理气活血汤, JLHD） on lipid peroxidative liver injury induced by alcohol. Methods： The rat alcoholic model of liver disease （ALD） induced by Lieber-DeCarli liquid diet was established. Thirty-two male SD rats were randomly divided into 4 groups ： the normal group （ n = 5）, the control group （ n = 9）, the model group （ n = 9） and the JLHD group （ n =9）. From the 4th week after modeling, the rats were given JLHD or distilled water by gastrogavage respectively, and the samples of blood and liver tissues were taken out from the rats for determination by the end of the 8th week. The hepatic pathological changes were observed with HE staining; the liver injury related indices, including activity of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in serum, γ-glutamyl transpeptidase （γ-GT） activity and triglyceride （TG） content in liver tissues, as well as the lipid peroxidation related indices, including malonaldehyde （MDA） content and nitric oxide synthase （NOS） activity in liver tissue, serum Fe^2＋ level, and the anti-peroxidation capacity related indices, including superoxide dismutase （SOD） activity, glutathion （GSH） content and reactive oxygen species （anti- ROS） activity in liver tissues were determined. Results： （ 1 ） There were obvious figures of fatty degeneration and inflammatory infiltration in liver tissues of the model group. As compared with the control group, in the model group, the activity of ALT and AST, and Fe^2＋ content in serum, γ-GT and NOS activity, TG and MDA content in liver tissues were significantly higher （ P〈0. 01 ）, while the activity of SOD, GSH and anti-ROS in liver tissues were significantly lower （P〈0.01）. （2） The fatty degeneration and inflammatory infiltration of liver tissues in the JLHD group were significantly lessen as compared with those in the model group; and the abnormalities of all the indexes revealed in the model rats were restored to certain extent in the JLHD group, and especially significant were the levels of ALT activity, MDA content and Fe^2＋ , which were nearly normal. Conclusion： JLHD has significant effects against alcoholic liver injury, the acting mechanism of which is likely to be related with its anti-lipid peroxidative effect.展开更多
目的观察理气化瘀汤对气滞血瘀型原发性痛经患者疗效的影响。方法采用随机临床对照研究方法,将148例患者分为对照组74例,治疗组74例。对照组予常规隔下逐瘀汤,治疗组予理气化瘀汤,通过比较VAS疼痛评分、COX痛经症状积分及用药安全性来...目的观察理气化瘀汤对气滞血瘀型原发性痛经患者疗效的影响。方法采用随机临床对照研究方法,将148例患者分为对照组74例,治疗组74例。对照组予常规隔下逐瘀汤,治疗组予理气化瘀汤,通过比较VAS疼痛评分、COX痛经症状积分及用药安全性来评价理气化瘀汤的临床疗效。结果经过3个月经周期的治疗,治疗组(理气化瘀汤)能明显降低患者VAS疼痛评分(3.83±1.52 vs 5.37±1.73),COX痛经症状积分(0.87±0.39 vs1.32±0.47;6.43±1.56 vs 8.87±1.73),与对照组比较差异具有统计学意义(P<0.05);同时治疗组对肝功能、肾功能无明显不良影响(P>0.05)。结论理气化瘀汤能有效安全缓解气滞血瘀型原发性痛经患者临床症状,改善患者生活质量。展开更多
基金Supported by the National Natural Science Foundation of China (No.30371818) and Project of Key Subject Construction of Shanghai (Y0302)
文摘Objective： To study the intervention effects of Jianpi Liqi Huoxue Decoction （健脾理气活血汤, JLHD） on lipid peroxidative liver injury induced by alcohol. Methods： The rat alcoholic model of liver disease （ALD） induced by Lieber-DeCarli liquid diet was established. Thirty-two male SD rats were randomly divided into 4 groups ： the normal group （ n = 5）, the control group （ n = 9）, the model group （ n = 9） and the JLHD group （ n =9）. From the 4th week after modeling, the rats were given JLHD or distilled water by gastrogavage respectively, and the samples of blood and liver tissues were taken out from the rats for determination by the end of the 8th week. The hepatic pathological changes were observed with HE staining; the liver injury related indices, including activity of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in serum, γ-glutamyl transpeptidase （γ-GT） activity and triglyceride （TG） content in liver tissues, as well as the lipid peroxidation related indices, including malonaldehyde （MDA） content and nitric oxide synthase （NOS） activity in liver tissue, serum Fe^2＋ level, and the anti-peroxidation capacity related indices, including superoxide dismutase （SOD） activity, glutathion （GSH） content and reactive oxygen species （anti- ROS） activity in liver tissues were determined. Results： （ 1 ） There were obvious figures of fatty degeneration and inflammatory infiltration in liver tissues of the model group. As compared with the control group, in the model group, the activity of ALT and AST, and Fe^2＋ content in serum, γ-GT and NOS activity, TG and MDA content in liver tissues were significantly higher （ P〈0. 01 ）, while the activity of SOD, GSH and anti-ROS in liver tissues were significantly lower （P〈0.01）. （2） The fatty degeneration and inflammatory infiltration of liver tissues in the JLHD group were significantly lessen as compared with those in the model group; and the abnormalities of all the indexes revealed in the model rats were restored to certain extent in the JLHD group, and especially significant were the levels of ALT activity, MDA content and Fe^2＋ , which were nearly normal. Conclusion： JLHD has significant effects against alcoholic liver injury, the acting mechanism of which is likely to be related with its anti-lipid peroxidative effect.
文摘目的观察理气化瘀汤对气滞血瘀型原发性痛经患者疗效的影响。方法采用随机临床对照研究方法,将148例患者分为对照组74例,治疗组74例。对照组予常规隔下逐瘀汤,治疗组予理气化瘀汤,通过比较VAS疼痛评分、COX痛经症状积分及用药安全性来评价理气化瘀汤的临床疗效。结果经过3个月经周期的治疗,治疗组(理气化瘀汤)能明显降低患者VAS疼痛评分(3.83±1.52 vs 5.37±1.73),COX痛经症状积分(0.87±0.39 vs1.32±0.47;6.43±1.56 vs 8.87±1.73),与对照组比较差异具有统计学意义(P<0.05);同时治疗组对肝功能、肾功能无明显不良影响(P>0.05)。结论理气化瘀汤能有效安全缓解气滞血瘀型原发性痛经患者临床症状,改善患者生活质量。
