Thermal sprayed Ni-5wt.% Al coating was fabricated on the substrate of 6061-T6 aluminum alloy by twin-wire arc spraying. Experimental results indicated that the average value of bond strength was around 46. 90 MPa, th...Thermal sprayed Ni-5wt.% Al coating was fabricated on the substrate of 6061-T6 aluminum alloy by twin-wire arc spraying. Experimental results indicated that the average value of bond strength was around 46. 90 MPa, the average hardness was 240 HV and the average value of surface roughness was about O. 14 mm. Friction and wear test results showed that the dry friction coefficient of Ni-5wt.% Al coating firstly decreased, and then tended to a slight increase after 200 cycles. In the early abrasion stage, adhesion wear played the key role for wear mechanics of Ni-5wt.% Al coating, but gradually abrasive wear became to replace adhesion wear.展开更多
Background：Human sulfatase-1 （Hsulf-l） is an endosulfatase that selectively removes sulfate groups from heparan sulfate proteoglycans （HSPGs）,altering the binding of several growth factors and cytokines to HSPG t...Background：Human sulfatase-1 （Hsulf-l） is an endosulfatase that selectively removes sulfate groups from heparan sulfate proteoglycans （HSPGs）,altering the binding of several growth factors and cytokines to HSPG to regulate cell proliferation,cell motility,and apoptosis.We investigated the role of combined cancer gene therapy with Hsulf-l and cytosine deaminase/5-fluorocytosine （CD/5-FC） suicide gene on a hepatocellular carcinoma （HCC） cell line,HepG2,in vitro and in vivo.Methods：Reverse transcription polymerase chain reaction and immunohistochemistry were used to determine the expression of Hsulf-1 in HCC.Cell apoptosis was observed through flow cytometry instrument and mechanism of Hsulf-1 to enhance the cytotoxicity of 5-FC against HCC was analyzed in HCC by confocal microscopy.We also establish a nude mice model of HCC to address the effect of Hsulf-1 expression on the CD/5-FC suicide gene therapy in vivo.Results：A significant decrease in HepG2 cell proliferation and an increase in HepG2 cell apoptosis were observed when Hsulf-1 expression was combined with the CD/5-FC gene suicide system.A noticeable bystander effect was observed when the Hsulf-1 and CD genes were co-expressed.Intracellular calcium was also increased after HepG2 cells were infected with the Hsulf-1 gene.In vivo studies showed that the suppression of tumor growth was more pronounced in animals treated with the Hsulf-1 plus CD than those treated with either gene therapy alone,and the combined treatment resulted in a significant increase in survival.Conclusions：Hsulf-1 expression combined with the CD/5-FC gene suicide system could be an effective treatment approach for HCC.展开更多
为比较1%苦参碱可溶液剂、2.5%鱼藤酮乳油和5%阿维菌素乳油对水稻稻纵卷叶螟的防治效果,于2016年进行上述3种生物药剂对水稻稻纵卷叶螟的田间药效试验。结果表明,可采用1%苦参碱可溶液剂1 800 m L/hm^2、2.5%鱼藤酮乳油3 750 m L/hm^2...为比较1%苦参碱可溶液剂、2.5%鱼藤酮乳油和5%阿维菌素乳油对水稻稻纵卷叶螟的防治效果,于2016年进行上述3种生物药剂对水稻稻纵卷叶螟的田间药效试验。结果表明,可采用1%苦参碱可溶液剂1 800 m L/hm^2、2.5%鱼藤酮乳油3 750 m L/hm^2或5%阿维菌素乳油375 m L/hm^2防治稻纵卷叶螟,且各种药剂对水稻安全。展开更多
文摘Thermal sprayed Ni-5wt.% Al coating was fabricated on the substrate of 6061-T6 aluminum alloy by twin-wire arc spraying. Experimental results indicated that the average value of bond strength was around 46. 90 MPa, the average hardness was 240 HV and the average value of surface roughness was about O. 14 mm. Friction and wear test results showed that the dry friction coefficient of Ni-5wt.% Al coating firstly decreased, and then tended to a slight increase after 200 cycles. In the early abrasion stage, adhesion wear played the key role for wear mechanics of Ni-5wt.% Al coating, but gradually abrasive wear became to replace adhesion wear.
文摘Background：Human sulfatase-1 （Hsulf-l） is an endosulfatase that selectively removes sulfate groups from heparan sulfate proteoglycans （HSPGs）,altering the binding of several growth factors and cytokines to HSPG to regulate cell proliferation,cell motility,and apoptosis.We investigated the role of combined cancer gene therapy with Hsulf-l and cytosine deaminase/5-fluorocytosine （CD/5-FC） suicide gene on a hepatocellular carcinoma （HCC） cell line,HepG2,in vitro and in vivo.Methods：Reverse transcription polymerase chain reaction and immunohistochemistry were used to determine the expression of Hsulf-1 in HCC.Cell apoptosis was observed through flow cytometry instrument and mechanism of Hsulf-1 to enhance the cytotoxicity of 5-FC against HCC was analyzed in HCC by confocal microscopy.We also establish a nude mice model of HCC to address the effect of Hsulf-1 expression on the CD/5-FC suicide gene therapy in vivo.Results：A significant decrease in HepG2 cell proliferation and an increase in HepG2 cell apoptosis were observed when Hsulf-1 expression was combined with the CD/5-FC gene suicide system.A noticeable bystander effect was observed when the Hsulf-1 and CD genes were co-expressed.Intracellular calcium was also increased after HepG2 cells were infected with the Hsulf-1 gene.In vivo studies showed that the suppression of tumor growth was more pronounced in animals treated with the Hsulf-1 plus CD than those treated with either gene therapy alone,and the combined treatment resulted in a significant increase in survival.Conclusions：Hsulf-1 expression combined with the CD/5-FC gene suicide system could be an effective treatment approach for HCC.
文摘为比较1%苦参碱可溶液剂、2.5%鱼藤酮乳油和5%阿维菌素乳油对水稻稻纵卷叶螟的防治效果,于2016年进行上述3种生物药剂对水稻稻纵卷叶螟的田间药效试验。结果表明,可采用1%苦参碱可溶液剂1 800 m L/hm^2、2.5%鱼藤酮乳油3 750 m L/hm^2或5%阿维菌素乳油375 m L/hm^2防治稻纵卷叶螟,且各种药剂对水稻安全。
