Many studies suggest that ethylene plays an important role in regulating metabolite synthesis. Dendrobium plants are traditional Chinese medicine and nowadays its medicinal components are known to be secondary metabol...Many studies suggest that ethylene plays an important role in regulating metabolite synthesis. Dendrobium plants are traditional Chinese medicine and nowadays its medicinal components are known to be secondary metabolites. In present study, a homolog of ACC oxidase （ACO） gene was isolated from flowers of Dendrobium officinale Kimura et Migo by PCR-method. The obtained cDNA of DoACO is 970 bp long and contains an open reading frame （ORP） encoding a protein with 314 amino acid residues. The DoACO shows high identity to its homologues from other plant species, that has 94.8% closest amino acid sequence of related protein with the ACO from Dendrobium hybrid cultivar. The putative ORP of the obtained sequence could encode a proper protein in respect of molecular weight under T -Lac promoter in E. coli.展开更多
文摘Many studies suggest that ethylene plays an important role in regulating metabolite synthesis. Dendrobium plants are traditional Chinese medicine and nowadays its medicinal components are known to be secondary metabolites. In present study, a homolog of ACC oxidase （ACO） gene was isolated from flowers of Dendrobium officinale Kimura et Migo by PCR-method. The obtained cDNA of DoACO is 970 bp long and contains an open reading frame （ORP） encoding a protein with 314 amino acid residues. The DoACO shows high identity to its homologues from other plant species, that has 94.8% closest amino acid sequence of related protein with the ACO from Dendrobium hybrid cultivar. The putative ORP of the obtained sequence could encode a proper protein in respect of molecular weight under T -Lac promoter in E. coli.
