Pinus elliottii is an exotic afforestation pine extensively distributed in southern parts of China.In order to understand whether endophytic fungi can affect seedling growth of P.elliottii,Piriformospora indica(Pi),Fu...Pinus elliottii is an exotic afforestation pine extensively distributed in southern parts of China.In order to understand whether endophytic fungi can affect seedling growth of P.elliottii,Piriformospora indica(Pi),Funnelifcrmis mosseae(Fm),and Diversispora tortuosa(Dt)were inoculated respectively,and the non-inoculated group was set as control.The growth indexes,the contents of soluble sugar and soluble protein,and plant endogenous hormone levels in the leaves of P.elliottii,were analyzed.The results showed that Fm,Dt and Pi colonized the P.elliottii roots to form mycorrhizal structure and chlamydospores arranged in beads respectively.Three fungal inoculants exhibited the stimulated growth responses,whilst Dt illustrated the most positive effect on plant height,single fresh weight,trunk diameter and root system structure,compared with the control.On the other hand,the soluble sugar and soluble protein contents were increased distinctively in mycorrhizal plants.The endogenous IAA,GA3,ZR contents were increased,while the ABA contents were reduced in mycorrhizal plants versus non-mycorrhizal plants.The fungi-induced endogenous hormone changes triggered plant growth improvement of P.elliottii seedlings.This research unraveled the positive effect of AM fungi and P.indica on growth of pine seedlings,while,more application of endophytic fungi to fields needs to be explored.展开更多
A pot experiment was conducted to determine the potential of AM fungi in phytoremediation of heavy metals and its effect on yield of wheat crop. The experiment was conducted in CR Design with four replications during ...A pot experiment was conducted to determine the potential of AM fungi in phytoremediation of heavy metals and its effect on yield of wheat crop. The experiment was conducted in CR Design with four replications during rabi 2012-13. Data showed no increase in grain and shoot yields by AMF inoculation with Zn, Cu, Fe, Mn at different levels but increased root yield, plant height, spike length and hundred grains weight of wheat as compared with uninoculated crop. Post-harvest soil Zn, Cu, Fe and Mn contents of 2, 4.4, 2.8 and 2.9 mg·kg-1, respectively were maximum in uninoculated plants treated with Zn, Cu, Fe, Mn at triple of recommended level. No increases in plant P, N, Zn, Cu, Fe and Mn uptakes were observed by the inoculation of AMF when compared with uninoculated crop. Maximum plant Zn, Cu, Fe and Mn uptakes of 160.5, 206, 1914.6 and 2653 g·ha-1, respectively were recorded in uninoculated plants applied with Zn, Cu, Fe, Mn at triple of recommended levels. Wheat roots infection intensity by AMF increased with higher AMF soil spores density. Results suggest the potential of phytoremediation of contaminated soil to be improved by the inoculation of crops with AMF.展开更多
在基质灭菌条件下接种丛枝菌根(AM)真菌能促进植物生长发育,但在未灭菌条件下接种AM真菌鲜有报道。因此,在温室盆栽条件下,以香橙砧木(Citrus Junossieb ex Tanaka)为材料,研究了不同处理基质(灭菌和未灭菌)及接种AM真菌对香橙砧木的根...在基质灭菌条件下接种丛枝菌根(AM)真菌能促进植物生长发育,但在未灭菌条件下接种AM真菌鲜有报道。因此,在温室盆栽条件下,以香橙砧木(Citrus Junossieb ex Tanaka)为材料,研究了不同处理基质(灭菌和未灭菌)及接种AM真菌对香橙砧木的根系菌根侵染率、株高、茎径、叶片数、生物量及根系性状的影响。探讨在模拟自然条件(基质未灭菌)下接种AM真菌其接种效应是否仍然有效。研究结果表明:在基质灭菌条件下,接种黄雷克囊霉(Redeckera fulvum)(R.f)处理的株高、叶片数、地上干重、生物量和根系性状显著优于未接种菌根处理,而接种凹坑无梗囊霉(Acaulospora excavata)(A.e)处理的株高、茎径、叶片数、生物量低于未接种处理,但无显著差异。而根系性状显著优于对照;在基质未灭菌条件下,除了接种R.f处理的总根长显著高于未接种处理外,其他指标均与未接种处理无显著差异。这表明在盆栽试验中基质灭菌条件下,接种R.f处理能提高香橙砧木生长,接种A.e处理能抑制其生长,但在基质未灭菌条件下,接种两种AM真菌的香橙砧木生长与对照无显著影响,没有表现出在基质灭菌条件下同等的接种效应。展开更多
Due to occasionally changing natural environment,plants are always threatened by drought, waterlogging ,high or low temperature,acidity,saline-alkali and so on.Therefore,the physiological,biochemical and ecological st...Due to occasionally changing natural environment,plants are always threatened by drought, waterlogging ,high or low temperature,acidity,saline-alkali and so on.Therefore,the physiological,biochemical and ecological studies on plants under environmental stress have become a hot field.Much research indicates that AM can improve the ability of plants to resist environmental stress.This article briefly reviewed the recent studies about the effects of AM on resistance of plants to environmental stress,especially to drought,saline and acidity,and the possible physiological and biochemical mechanisms are discussed as well.展开更多
为了阐明荒漠柠条锦鸡儿(Caragana korshinskii Kom.)根围AM真菌群落组成及其分布特点,2010年7月从河北省与内蒙古交界荒漠带选择多伦湖、黑城子和二羊点3个样地,按0—10、10—20、20—30、30—40、40—50 cm 5个土层采集柠条根围土壤样...为了阐明荒漠柠条锦鸡儿(Caragana korshinskii Kom.)根围AM真菌群落组成及其分布特点,2010年7月从河北省与内蒙古交界荒漠带选择多伦湖、黑城子和二羊点3个样地,按0—10、10—20、20—30、30—40、40—50 cm 5个土层采集柠条根围土壤样品,研究了柠条根围AM真菌物种组成、生态分布及与土壤因子的相关性。共分离鉴定出4属24种AM真菌,其中球囊霉属(Glomus)16种,是3个样地共同优势属;无梗囊霉属(Acaulospora)5种,是3个样地共有属;盾巨孢囊霉属(Scutellospora)2种;多孢囊霉属(Diversispora)1种;网状球囊霉(G.reticulatum)是黑城子和多伦湖样地优势种,二羊点亚优势种;黑球囊霉(G.melanosporum)是黑城子和二羊点样地优势种,多伦湖亚优势种;二羊点的AM真菌种类和孢子密度最高;黑城子和多伦湖样地无明显差异。AM真菌孢子密度与土壤碱解N、有机质和速效P含量极显著正相关,其中土壤碱解N影响最大。结果表明,柠条根围AM真菌物种多样性丰富,具有明显空间异质性,并与土壤因子关系密切,这为进一步分离筛选优势AM真菌菌种,充分利用AM真菌资源促进荒漠柠条生长提供了依据。展开更多
【目的】研究盐碱胁迫下丛枝菌根(AM)真菌对沙枣苗木的生长和生理的影响,以期探索AM真菌提高沙枣苗木耐盐碱机制,为菌根化沙枣苗木在盐碱地推广应用提供理论基础。【方法】采用盆栽法研究了4种不同土壤盐碱度下分别接种根内球囊霉和摩...【目的】研究盐碱胁迫下丛枝菌根(AM)真菌对沙枣苗木的生长和生理的影响,以期探索AM真菌提高沙枣苗木耐盐碱机制,为菌根化沙枣苗木在盐碱地推广应用提供理论基础。【方法】采用盆栽法研究了4种不同土壤盐碱度下分别接种根内球囊霉和摩西球囊霉对沙枣苗木的生长影响,并对沙枣苗木的光合特性、Na+和K+含量、丙二醛含量、脯氨酸含量等各项生理指标进行测定。【结果】2种AM真菌均能与沙枣苗木建立共生关系,且GI处理对苗木的侵染率显著高于接种GM处理,但随着土壤中盐浓度增加,沙枣苗木菌根侵染率有所降低。在同一盐碱度下接种AM真菌可显著促进沙枣幼苗的生长,在中度盐碱胁迫下(含盐量1.56%,p H 9.52),接种GI和GM的沙枣苗木株高分别较未接种AM真菌植株增加了20.07%,9.68%,植株干生物量显著增加;AM真菌可显著提高盐碱胁迫下沙枣幼苗叶片叶绿素含量和光合生理特性,其叶片净光合速率Pn、蒸腾速率Tr、气孔导度Gs、胞间二氧化碳浓度Ci均显著高于未接种AM真菌处理的幼苗(P<0.05)。接种GI和GM处理植株叶片组织内SOD,CAT和POD酶活性显著高于对照处理,但根系及叶片组织内丙二醛含量显著低于对照处理的植株,而其脯氨酸含量却都表现出显著提高。接种AM真菌沙枣根系和叶片中Na+含量较未接种处理植株显著下降,K+含量和K+/Na+比值显著提高。【结论】接种AM真菌能显著提高沙枣苗木耐盐碱能力。接种根内球囊霉的沙枣苗木的生长及抗盐碱胁迫能力的各项生理指标均显著高于接种摩西球囊霉处理的苗木,表明根内球囊霉在盐碱地改良方面具有很好的推广应用前景。展开更多
基金We would like to thank the fund support of the Ideological and Political Course and Curriculum and Ideological and Political Demonstration Construction Project of Yangtze University(2020,No.65)We would also thank the help provided by the Open Fund of Institute of Root Biology of Yangtze University,which supported the purchase of AM fungi strain from Institute of Plant Nutrition and Resources,Beijing Academy of Agriculture and Forestry Sciences.
文摘Pinus elliottii is an exotic afforestation pine extensively distributed in southern parts of China.In order to understand whether endophytic fungi can affect seedling growth of P.elliottii,Piriformospora indica(Pi),Funnelifcrmis mosseae(Fm),and Diversispora tortuosa(Dt)were inoculated respectively,and the non-inoculated group was set as control.The growth indexes,the contents of soluble sugar and soluble protein,and plant endogenous hormone levels in the leaves of P.elliottii,were analyzed.The results showed that Fm,Dt and Pi colonized the P.elliottii roots to form mycorrhizal structure and chlamydospores arranged in beads respectively.Three fungal inoculants exhibited the stimulated growth responses,whilst Dt illustrated the most positive effect on plant height,single fresh weight,trunk diameter and root system structure,compared with the control.On the other hand,the soluble sugar and soluble protein contents were increased distinctively in mycorrhizal plants.The endogenous IAA,GA3,ZR contents were increased,while the ABA contents were reduced in mycorrhizal plants versus non-mycorrhizal plants.The fungi-induced endogenous hormone changes triggered plant growth improvement of P.elliottii seedlings.This research unraveled the positive effect of AM fungi and P.indica on growth of pine seedlings,while,more application of endophytic fungi to fields needs to be explored.
文摘A pot experiment was conducted to determine the potential of AM fungi in phytoremediation of heavy metals and its effect on yield of wheat crop. The experiment was conducted in CR Design with four replications during rabi 2012-13. Data showed no increase in grain and shoot yields by AMF inoculation with Zn, Cu, Fe, Mn at different levels but increased root yield, plant height, spike length and hundred grains weight of wheat as compared with uninoculated crop. Post-harvest soil Zn, Cu, Fe and Mn contents of 2, 4.4, 2.8 and 2.9 mg·kg-1, respectively were maximum in uninoculated plants treated with Zn, Cu, Fe, Mn at triple of recommended level. No increases in plant P, N, Zn, Cu, Fe and Mn uptakes were observed by the inoculation of AMF when compared with uninoculated crop. Maximum plant Zn, Cu, Fe and Mn uptakes of 160.5, 206, 1914.6 and 2653 g·ha-1, respectively were recorded in uninoculated plants applied with Zn, Cu, Fe, Mn at triple of recommended levels. Wheat roots infection intensity by AMF increased with higher AMF soil spores density. Results suggest the potential of phytoremediation of contaminated soil to be improved by the inoculation of crops with AMF.
文摘Due to occasionally changing natural environment,plants are always threatened by drought, waterlogging ,high or low temperature,acidity,saline-alkali and so on.Therefore,the physiological,biochemical and ecological studies on plants under environmental stress have become a hot field.Much research indicates that AM can improve the ability of plants to resist environmental stress.This article briefly reviewed the recent studies about the effects of AM on resistance of plants to environmental stress,especially to drought,saline and acidity,and the possible physiological and biochemical mechanisms are discussed as well.
文摘为了阐明荒漠柠条锦鸡儿(Caragana korshinskii Kom.)根围AM真菌群落组成及其分布特点,2010年7月从河北省与内蒙古交界荒漠带选择多伦湖、黑城子和二羊点3个样地,按0—10、10—20、20—30、30—40、40—50 cm 5个土层采集柠条根围土壤样品,研究了柠条根围AM真菌物种组成、生态分布及与土壤因子的相关性。共分离鉴定出4属24种AM真菌,其中球囊霉属(Glomus)16种,是3个样地共同优势属;无梗囊霉属(Acaulospora)5种,是3个样地共有属;盾巨孢囊霉属(Scutellospora)2种;多孢囊霉属(Diversispora)1种;网状球囊霉(G.reticulatum)是黑城子和多伦湖样地优势种,二羊点亚优势种;黑球囊霉(G.melanosporum)是黑城子和二羊点样地优势种,多伦湖亚优势种;二羊点的AM真菌种类和孢子密度最高;黑城子和多伦湖样地无明显差异。AM真菌孢子密度与土壤碱解N、有机质和速效P含量极显著正相关,其中土壤碱解N影响最大。结果表明,柠条根围AM真菌物种多样性丰富,具有明显空间异质性,并与土壤因子关系密切,这为进一步分离筛选优势AM真菌菌种,充分利用AM真菌资源促进荒漠柠条生长提供了依据。
文摘【目的】研究盐碱胁迫下丛枝菌根(AM)真菌对沙枣苗木的生长和生理的影响,以期探索AM真菌提高沙枣苗木耐盐碱机制,为菌根化沙枣苗木在盐碱地推广应用提供理论基础。【方法】采用盆栽法研究了4种不同土壤盐碱度下分别接种根内球囊霉和摩西球囊霉对沙枣苗木的生长影响,并对沙枣苗木的光合特性、Na+和K+含量、丙二醛含量、脯氨酸含量等各项生理指标进行测定。【结果】2种AM真菌均能与沙枣苗木建立共生关系,且GI处理对苗木的侵染率显著高于接种GM处理,但随着土壤中盐浓度增加,沙枣苗木菌根侵染率有所降低。在同一盐碱度下接种AM真菌可显著促进沙枣幼苗的生长,在中度盐碱胁迫下(含盐量1.56%,p H 9.52),接种GI和GM的沙枣苗木株高分别较未接种AM真菌植株增加了20.07%,9.68%,植株干生物量显著增加;AM真菌可显著提高盐碱胁迫下沙枣幼苗叶片叶绿素含量和光合生理特性,其叶片净光合速率Pn、蒸腾速率Tr、气孔导度Gs、胞间二氧化碳浓度Ci均显著高于未接种AM真菌处理的幼苗(P<0.05)。接种GI和GM处理植株叶片组织内SOD,CAT和POD酶活性显著高于对照处理,但根系及叶片组织内丙二醛含量显著低于对照处理的植株,而其脯氨酸含量却都表现出显著提高。接种AM真菌沙枣根系和叶片中Na+含量较未接种处理植株显著下降,K+含量和K+/Na+比值显著提高。【结论】接种AM真菌能显著提高沙枣苗木耐盐碱能力。接种根内球囊霉的沙枣苗木的生长及抗盐碱胁迫能力的各项生理指标均显著高于接种摩西球囊霉处理的苗木,表明根内球囊霉在盐碱地改良方面具有很好的推广应用前景。
