Actinidia eriantha is one of the species of kiwifruit with a particularly high ascorbic acid (AsA) content.However, the molecular mechanism driving AsA richness in fruit remains unclear.In order to reveal the molecula...Actinidia eriantha is one of the species of kiwifruit with a particularly high ascorbic acid (AsA) content.However, the molecular mechanism driving AsA richness in fruit remains unclear.In order to reveal the molecular mechanism of AsA richness in A. eriantha, this study constructed a regulatory network related to AsA metabolism by combining genomics,metabolomics and transcriptomics.We assembled a high-quality genome of A. eriantha ‘Ganlv 1’ with only five remaining gaps.The assembly is comprised of 29 pseudochromosomes with a total size of 615.95 Mb, and contig N50 of 20.35 Mb.Among them,24 of the pseudochromosomes were obtained directly from telomere-to-telomere.The LTR assembly index score and consensus quality value were 21.34 and 39.90%, respectively.Subsequently, 61 metabolites and 2 092genes were found to be differentially accumulated/expressed during fruit development by metabolome and transcriptome assays, respectively.AsA metabolism and the cyclic regeneration pathway were found to have high expression levels throughout fruit growth and development,suggesting its crucial role in the regulation of AsA.Furthermore,the AsA contents are highly associated with ascorbate peroxidase genes.The genome obtained in this study provides genomic resources for the genetic and breeding research ofA. eriantha, and the constructed regulatory network can provide a public data platform for future research on kiwifruit.展开更多
This study aimed to evaluate the anti-hyperglycemic and antioxidant role of Actinidia deliciosa(kiwifruits)aqueous extract in streptozotocin-treated rats.Animals were distributed into;control,A.deliciosa aqueous extra...This study aimed to evaluate the anti-hyperglycemic and antioxidant role of Actinidia deliciosa(kiwifruits)aqueous extract in streptozotocin-treated rats.Animals were distributed into;control,A.deliciosa aqueous extract(ADAE;1 g/kg orally),streptozotocin(STZ;50 mg/kg,i.p,single dose),and STZ plus ADAE,respectively.Results showed that ADAE had high antioxidant and radical scavenging potency.Elevation in blood sugar level,lipid peroxidation(LPO),kidney function biomarkers,and perturbations in hematological parameters were observed in diabetic rats.While,enzymatic and non-enzymatic antioxidants,protein content,and alkaline phosphatase(ALP)activity declined.Furthermore,histological,immunohistochemical alpha-smooth muscle actin immunoreactivity(α-SMA-ir)and histochemical(collagen,total protein,DNA,and RNA)alterations were observed in rat kidneys.Moreover,STZ produced upregulation of inflammatory associated genes(tumor necrosis factor-alpha;TNF-αand transforming growth factorβ1;TGF-β1)and triggered apoptosis by upregulating apoptotic related gene[Bcl2-associated X protein(Bax)]and downregulating anti-apoptotic related gene B-cell lymphoma-2(Bcl-2)based on real-time PCR data.Moreover,diabetic rats administered with ADAE showed significant restoration in LPO,antioxidant status,and biochemical indices besides tissue architecture,and genes improvement regarding STZ group.Conclusively,A.deliciosa has a valuable ameliorative infl uence and can restore glucose levels and improve kidney dysfunction in diabetic rats.展开更多
This study aimed to investigate the protective effects of fleshes from two Actinidia chinensis(ACF), pericarps from two A. chinensis(ACP), and fleshes with pericarps from two A. chinensis(ACFP)on high fructose(HF)-ins...This study aimed to investigate the protective effects of fleshes from two Actinidia chinensis(ACF), pericarps from two A. chinensis(ACP), and fleshes with pericarps from two A. chinensis(ACFP)on high fructose(HF)-instigated dyslipidemia, hepatic steatosis, oxidative stress, insulin resistance, and fatty acid metabolism disorders in rats. In general, the above abnormalities were improved after 10 weeks intervention of ACF, ACP, and ACFP. Especially, ACFP considerably ameliorated HF-induced abnormal changes in body weight gain, serum TC, TG, LDL-C and HDL-C levels, as well as serum and hepatic SFAs, MUFAs and PUFAs contents. ACFP also alleviated HF-induced hyperglycemia and hyperinsulinemia, stabilized HF-caused increase in hepatic MDA and serum ALT, AST levels, and restored HF-declined hepatic T-SOD and GSH-Px activities. Besides, histopathology of the liver further endorsed the protective effects of ACFP on hepatocellular injury. Moreover, ACFP increased HF-dropped acetic, propionic and butyric acid levels. Overall, ACFP employs more efficacious protective effects against HF-induced metabolic disorders and liver damage than ACF and ACP. This study delivers a scientific foundation for developing kiwifruit(counting peel)-based dietary supplements for those with glucolipid-metabolic disorders and liver damage.展开更多
Background:Actinidia chinensis Planch.roots(AcRoots)have been applied as an anti-inflammatory and antitumor drug in the treatment of gastric cancer(GC).However,their mechanisms against GC cells remain unclear.To inves...Background:Actinidia chinensis Planch.roots(AcRoots)have been applied as an anti-inflammatory and antitumor drug in the treatment of gastric cancer(GC).However,their mechanisms against GC cells remain unclear.To investigate the anticancer effect of AcRoots in GC and the possible underlying mechanism by using network pharmacology.Methods:Differentially expressed genes between gastric precancerous lesions and cancer were analyzed in Gene Expression Omnibus datasets,and these genes were overlapped with potential targets of AcRoots.Potential targets and pathways for AcRoots treatment of GC predicted by network pharmacology.Furthermore,we used the GC cell line HGC27 to explore the molecular mechanisms in the context of hub genes in apoptosis,invasion,metastasis,and epithelial to mesenchymal transition-promoting factors.Molecular docking between hub targets and active drug components was also performed.Results:Network pharmacological analysis suggested that the potential mechanism was related to the Wnt pathway and predicted nine hub genes.In in vitro studies,AcRoots significantly decreased HGC27 cell viability and promoted apoptosis by upregulating caspase3 and downregulating Bcl2.Moreover,it suppressed invasion and metastasis as well as the expression of epithelial to mesenchymal transition-related factors.In addition,AcRoots affected the phosphorylation level of GSK3β(Ser9)in the Wnt pathway to promote the degradation ofβ-catenin,resulting in the downregulation of the downstream target genes c-myc,cyclin D1 and snail.All the experimental results were consistent with the network pharmacology results.Conclusion:This study combined network pharmacology with in vitro experiments to provide valid evidence for the clinical promotion of AcRoots.展开更多
Actinidia chinensis(kiwifruit)is a perennial horticultural crop species of the Actinidiaceae family with high nutritional and economic value.Two versions of the A.chinensis genomes have been previously assembled,based...Actinidia chinensis(kiwifruit)is a perennial horticultural crop species of the Actinidiaceae family with high nutritional and economic value.Two versions of the A.chinensis genomes have been previously assembled,based mainly on relatively short reads.Here,we report an improved chromosome-level reference genome of A.chinensis(v3.0),based mainly on PacBio long reads and Hi-C data.The high-quality assembled genome is 653 Mb long,with 0.76%heterozygosity.At least 43%of the genome consists of repetitive sequences,and the most abundant long terminal repeats were further identified and account for 23.38%of our novel genome.It has clear improvements in contiguity,accuracy,and gene annotation over the two previous versions and contains 40,464 annotated protein-coding genes,of which 94.41%are functionally annotated.Moreover,further analyses of genetic collinearity revealed that the kiwifruit genome has undergone two whole-genome duplications:one affecting all Ericales families near the K-T extinction event and a recent genus-specific duplication.The reference genome presented here will be highly useful for further molecular elucidation of diverse traits and for the breeding of this horticultural crop,as well as evolutionary studies with related taxa.展开更多
Anthocyanins are a group of secondary metabolites that colour fruit and flowers orange,red,purple or blue depending on a number of factors,such as the basic structure,co-pigmentation,metal ion complexation and vacuola...Anthocyanins are a group of secondary metabolites that colour fruit and flowers orange,red,purple or blue depending on a number of factors,such as the basic structure,co-pigmentation,metal ion complexation and vacuolar pH.The biosynthesis of anthocyanin is regulated at the transcriptional level by a group of transcription factors,the MYB–bHLH–WD40(MBW)complex.In this study,the purple colouration in several kiwifruit(Actinidia)species was identified and characterised as red cyanidin-based and blue delphinidin-based anthocyanins.The differential pigmentation in the skin and flesh can be attributed to the differential ratio of cyanidin and delphinidin derivatives accumulated in the total anthocyanin profile.The expression of anthocyanin biosynthetic genes chalcone synthase(CHS),flavonoid 3-O-glucosyltransferase(F3GT),flavonoid 3′-hydroxylase(F3′H)and flavonoid 3′5′-hydroxylase(F3′5′H)is crucial for anthocyanin accumulation.However,the balance of expression of the F3′H and F3′5′H genes appears responsible for the ratio of cyanidin and delphinidin derivatives,while a lack of CHS,F3GT and MYB110 expression is responsible for a lack of total anthocyanins.The transcriptional regulation of the F3′H and F3′5′H promoters by the R2R3 MYB transcription factor MYB110 is markedly different in tobacco transient assays.When kiwifruit MYB10 or MYB110 are over-expressed in Actinidia chinensis both cyanidin-based and delphinidin-based anthocyanins are elevated,but F3′H and F3′5′H genes are not strongly correlated with MYB expression.These results suggest that the core kiwifruit anthocyanin pathway genes are dependent on characterised MYB transcription factors,while other regulatory proteins are more directly responsible for the expression of the F3′H and F3′5′H genes.展开更多
Actinidia arguta is a berry fruit with high nutritional values.To evaluate the comprehensive quality,the fruit weight,titratable acid(TAC),soluble solids(SSC),vitamin C,mineral elements,and taste value of67 accessions...Actinidia arguta is a berry fruit with high nutritional values.To evaluate the comprehensive quality,the fruit weight,titratable acid(TAC),soluble solids(SSC),vitamin C,mineral elements,and taste value of67 accessions of A.arguta were measured,and sensory attributes including olfactive intensity,juiciness,sweetness,sourness,and astringency were assessed.Cluster analysis was carried out based on mineral elements,and principal component analysis(PCA)was used for comprehensive score ranking.The result demonstrated that fruit weight ranged from 3.5 g to 24.2 g,SSC ranged from 11.94%to 20.56%,TAC ranged from 0.15%to 1.20%,and vitamin C ranged from 3.51 g/kg to 230.76 g/kg(fresh weight)among the tested samples.There were significant differences in sourness,sweetness,bitterness,and umami among the 67 accessions based on electronic tongue analysis,and this result was rapidly and objectively characterized by the sensory assessment.The dendrogram was obtained and clustered the accessions into two major clusters,and five principal components were extracted by PCA.Overall,the information provided by this study identified the 10 best germplasm resources for fresh-eating and processing fruits.It can provide theoretical reference for nutritional efficacy evaluation and food development of A.arguta.展开更多
Exogenous application of a cytokinin-like compound forchlorfenuron(CPPU)can promote fruit growth,although often at the expense of dry matter(DM),an important indicator of fruit quality.Actinidia chinensis var.delicios...Exogenous application of a cytokinin-like compound forchlorfenuron(CPPU)can promote fruit growth,although often at the expense of dry matter(DM),an important indicator of fruit quality.Actinidia chinensis var.deliciosa‘Hayward’fruit are very responsive to CPPU treatments,but the mechanism underlying the significant fruit weight increase and associated decrease in DM is unclear.In this study,we hypothesised that CPPU-enhanced growth increases fruit carbohydrate demand,but limited carbohydrate supply resulted in decreased fruit DM.During fruit development,CPPU effects on physical parameters,metabolites,osmotic pressure and transcriptional changes were assessed under conditions of both standard and a high carbohydrate supply.We showed that CPPU increased fruit fresh weight but the dramatic DM decrease was not carbohydrate limited.Enhanced glucose and fructose concentrations contributed to an increase in soluble carbohydrate osmotic pressure,which was correlated with increased water accumulation in CPPU-treated fruit and up-regulation of water channel aquaporin gene PIP2.4 at 49 days after anthesis.Transcipt analysis suggested that the molecular mechanism contributing to increased glucose and fructose concentrations was altered by carbohydrate supply.At standard carbohydrate supply,the early glucose increase in CPPU fruit was associated with reduced starch synthesis and increased starch degradation.When carbohydrate supply was high,the early glucose increase in CPPU fruit was associated with a general decrease in starch synthesis but up-regulation of vacuolar invertase and fructokinase genes.We conclude that CPPU affected fruit expansion by increasing the osmotically-driven water uptake and its effect was not carbohydrate supply-limited.展开更多
Our study was conducted to determine In vitro germination ability of pollens from 25 wild Actinidia eriantha genotypes after one year freeze storage, afterwards we examined fertilization ability of stored pollens of 8...Our study was conducted to determine In vitro germination ability of pollens from 25 wild Actinidia eriantha genotypes after one year freeze storage, afterwards we examined fertilization ability of stored pollens of 8 genotypes selected according to In vitro test results from aforementioned 25 genotypes, and finally investigated effects of stored viable pollens from “MH67”, “MH55”, “MH48” and “MH45” genotypes on fruit quality characters of the female experimental plant “M3” as the main purpose. Non-pollinated “M3” kiwifruit plant was taken as control. We found that In vitro germination and fertilization ability of different stored pollen sources, and also fruit quality characters including dry matter, total sugar, titratable acid, vitamin C, total polyphenol, total flavonoid, chlorophyll (“a”, “b”, and total) and carotenoid contents except total soluble solid content were significantly different. MH67 and MH45 genotypes were evaluated as the most suitable pollenizers which can be recommended as new candidate cultivars because of long term storage ability and brought about desired fruit quality characters. They also will be investigated more at further breeding studies.展开更多
基金supported by the National Natural Science Foundation of China (32160692 and 31760559)the Key Research and Development Plan from Jiangxi Science and Technology Department, China (20192ACB60002)。
文摘Actinidia eriantha is one of the species of kiwifruit with a particularly high ascorbic acid (AsA) content.However, the molecular mechanism driving AsA richness in fruit remains unclear.In order to reveal the molecular mechanism of AsA richness in A. eriantha, this study constructed a regulatory network related to AsA metabolism by combining genomics,metabolomics and transcriptomics.We assembled a high-quality genome of A. eriantha ‘Ganlv 1’ with only five remaining gaps.The assembly is comprised of 29 pseudochromosomes with a total size of 615.95 Mb, and contig N50 of 20.35 Mb.Among them,24 of the pseudochromosomes were obtained directly from telomere-to-telomere.The LTR assembly index score and consensus quality value were 21.34 and 39.90%, respectively.Subsequently, 61 metabolites and 2 092genes were found to be differentially accumulated/expressed during fruit development by metabolome and transcriptome assays, respectively.AsA metabolism and the cyclic regeneration pathway were found to have high expression levels throughout fruit growth and development,suggesting its crucial role in the regulation of AsA.Furthermore,the AsA contents are highly associated with ascorbate peroxidase genes.The genome obtained in this study provides genomic resources for the genetic and breeding research ofA. eriantha, and the constructed regulatory network can provide a public data platform for future research on kiwifruit.
文摘This study aimed to evaluate the anti-hyperglycemic and antioxidant role of Actinidia deliciosa(kiwifruits)aqueous extract in streptozotocin-treated rats.Animals were distributed into;control,A.deliciosa aqueous extract(ADAE;1 g/kg orally),streptozotocin(STZ;50 mg/kg,i.p,single dose),and STZ plus ADAE,respectively.Results showed that ADAE had high antioxidant and radical scavenging potency.Elevation in blood sugar level,lipid peroxidation(LPO),kidney function biomarkers,and perturbations in hematological parameters were observed in diabetic rats.While,enzymatic and non-enzymatic antioxidants,protein content,and alkaline phosphatase(ALP)activity declined.Furthermore,histological,immunohistochemical alpha-smooth muscle actin immunoreactivity(α-SMA-ir)and histochemical(collagen,total protein,DNA,and RNA)alterations were observed in rat kidneys.Moreover,STZ produced upregulation of inflammatory associated genes(tumor necrosis factor-alpha;TNF-αand transforming growth factorβ1;TGF-β1)and triggered apoptosis by upregulating apoptotic related gene[Bcl2-associated X protein(Bax)]and downregulating anti-apoptotic related gene B-cell lymphoma-2(Bcl-2)based on real-time PCR data.Moreover,diabetic rats administered with ADAE showed significant restoration in LPO,antioxidant status,and biochemical indices besides tissue architecture,and genes improvement regarding STZ group.Conclusively,A.deliciosa has a valuable ameliorative infl uence and can restore glucose levels and improve kidney dysfunction in diabetic rats.
基金funded by the National Natural Science Foundation of China (C31901702,and 32072175)the China Postdoctoral Science Foundation (2019M653535,2020T130395)+2 种基金the Fundamental Research Funds for the Central Universities of Shaanxi Normal University in China (GK202003083)the grants of Sci-Tech Innovation Team of Shaanxi Province (2019TD-035)the Development Program for Innovative Research Team of Shaanxi Normal University (GK202101006)。
文摘This study aimed to investigate the protective effects of fleshes from two Actinidia chinensis(ACF), pericarps from two A. chinensis(ACP), and fleshes with pericarps from two A. chinensis(ACFP)on high fructose(HF)-instigated dyslipidemia, hepatic steatosis, oxidative stress, insulin resistance, and fatty acid metabolism disorders in rats. In general, the above abnormalities were improved after 10 weeks intervention of ACF, ACP, and ACFP. Especially, ACFP considerably ameliorated HF-induced abnormal changes in body weight gain, serum TC, TG, LDL-C and HDL-C levels, as well as serum and hepatic SFAs, MUFAs and PUFAs contents. ACFP also alleviated HF-induced hyperglycemia and hyperinsulinemia, stabilized HF-caused increase in hepatic MDA and serum ALT, AST levels, and restored HF-declined hepatic T-SOD and GSH-Px activities. Besides, histopathology of the liver further endorsed the protective effects of ACFP on hepatocellular injury. Moreover, ACFP increased HF-dropped acetic, propionic and butyric acid levels. Overall, ACFP employs more efficacious protective effects against HF-induced metabolic disorders and liver damage than ACF and ACP. This study delivers a scientific foundation for developing kiwifruit(counting peel)-based dietary supplements for those with glucolipid-metabolic disorders and liver damage.
基金funded by the National Natural Science Foundation of China(Project No.81973615,No.81803910).
文摘Background:Actinidia chinensis Planch.roots(AcRoots)have been applied as an anti-inflammatory and antitumor drug in the treatment of gastric cancer(GC).However,their mechanisms against GC cells remain unclear.To investigate the anticancer effect of AcRoots in GC and the possible underlying mechanism by using network pharmacology.Methods:Differentially expressed genes between gastric precancerous lesions and cancer were analyzed in Gene Expression Omnibus datasets,and these genes were overlapped with potential targets of AcRoots.Potential targets and pathways for AcRoots treatment of GC predicted by network pharmacology.Furthermore,we used the GC cell line HGC27 to explore the molecular mechanisms in the context of hub genes in apoptosis,invasion,metastasis,and epithelial to mesenchymal transition-promoting factors.Molecular docking between hub targets and active drug components was also performed.Results:Network pharmacological analysis suggested that the potential mechanism was related to the Wnt pathway and predicted nine hub genes.In in vitro studies,AcRoots significantly decreased HGC27 cell viability and promoted apoptosis by upregulating caspase3 and downregulating Bcl2.Moreover,it suppressed invasion and metastasis as well as the expression of epithelial to mesenchymal transition-related factors.In addition,AcRoots affected the phosphorylation level of GSK3β(Ser9)in the Wnt pathway to promote the degradation ofβ-catenin,resulting in the downregulation of the downstream target genes c-myc,cyclin D1 and snail.All the experimental results were consistent with the network pharmacology results.Conclusion:This study combined network pharmacology with in vitro experiments to provide valid evidence for the clinical promotion of AcRoots.
基金supported by the National Key Research and Development Program of China(ref.2017YFC0505203)Fundamental Research Funds for the Central Universities(ref.2018CDDY-S02-SCU)+1 种基金National High-Level Talents Special Support Plan(10 Thousand Talents Plan)985 and 211 Projects of Sichuan University.
文摘Actinidia chinensis(kiwifruit)is a perennial horticultural crop species of the Actinidiaceae family with high nutritional and economic value.Two versions of the A.chinensis genomes have been previously assembled,based mainly on relatively short reads.Here,we report an improved chromosome-level reference genome of A.chinensis(v3.0),based mainly on PacBio long reads and Hi-C data.The high-quality assembled genome is 653 Mb long,with 0.76%heterozygosity.At least 43%of the genome consists of repetitive sequences,and the most abundant long terminal repeats were further identified and account for 23.38%of our novel genome.It has clear improvements in contiguity,accuracy,and gene annotation over the two previous versions and contains 40,464 annotated protein-coding genes,of which 94.41%are functionally annotated.Moreover,further analyses of genetic collinearity revealed that the kiwifruit genome has undergone two whole-genome duplications:one affecting all Ericales families near the K-T extinction event and a recent genus-specific duplication.The reference genome presented here will be highly useful for further molecular elucidation of diverse traits and for the breeding of this horticultural crop,as well as evolutionary studies with related taxa.
基金Research is supported by The New Zealand Government,Ministry of Business,Innovation and Employment Endeavour Fund(MBIE),‘Turbo Breeding’(C11X1602)and MBIE‘Filling the Void’(C11X1704).YP is supported by the Joint Graduate School in Plant and Food Science,University of Auckland.
文摘Anthocyanins are a group of secondary metabolites that colour fruit and flowers orange,red,purple or blue depending on a number of factors,such as the basic structure,co-pigmentation,metal ion complexation and vacuolar pH.The biosynthesis of anthocyanin is regulated at the transcriptional level by a group of transcription factors,the MYB–bHLH–WD40(MBW)complex.In this study,the purple colouration in several kiwifruit(Actinidia)species was identified and characterised as red cyanidin-based and blue delphinidin-based anthocyanins.The differential pigmentation in the skin and flesh can be attributed to the differential ratio of cyanidin and delphinidin derivatives accumulated in the total anthocyanin profile.The expression of anthocyanin biosynthetic genes chalcone synthase(CHS),flavonoid 3-O-glucosyltransferase(F3GT),flavonoid 3′-hydroxylase(F3′H)and flavonoid 3′5′-hydroxylase(F3′5′H)is crucial for anthocyanin accumulation.However,the balance of expression of the F3′H and F3′5′H genes appears responsible for the ratio of cyanidin and delphinidin derivatives,while a lack of CHS,F3GT and MYB110 expression is responsible for a lack of total anthocyanins.The transcriptional regulation of the F3′H and F3′5′H promoters by the R2R3 MYB transcription factor MYB110 is markedly different in tobacco transient assays.When kiwifruit MYB10 or MYB110 are over-expressed in Actinidia chinensis both cyanidin-based and delphinidin-based anthocyanins are elevated,but F3′H and F3′5′H genes are not strongly correlated with MYB expression.These results suggest that the core kiwifruit anthocyanin pathway genes are dependent on characterised MYB transcription factors,while other regulatory proteins are more directly responsible for the expression of the F3′H and F3′5′H genes.
基金supported by Special Scientific Research Fund of Agricultural Public Welfare Profession of China[grant number 200903013]Liaoning Province,Shenyang Agricultural University,high-end talent introduction fund project[grant number SYAU20160003]。
文摘Actinidia arguta is a berry fruit with high nutritional values.To evaluate the comprehensive quality,the fruit weight,titratable acid(TAC),soluble solids(SSC),vitamin C,mineral elements,and taste value of67 accessions of A.arguta were measured,and sensory attributes including olfactive intensity,juiciness,sweetness,sourness,and astringency were assessed.Cluster analysis was carried out based on mineral elements,and principal component analysis(PCA)was used for comprehensive score ranking.The result demonstrated that fruit weight ranged from 3.5 g to 24.2 g,SSC ranged from 11.94%to 20.56%,TAC ranged from 0.15%to 1.20%,and vitamin C ranged from 3.51 g/kg to 230.76 g/kg(fresh weight)among the tested samples.There were significant differences in sourness,sweetness,bitterness,and umami among the 67 accessions based on electronic tongue analysis,and this result was rapidly and objectively characterized by the sensory assessment.The dendrogram was obtained and clustered the accessions into two major clusters,and five principal components were extracted by PCA.Overall,the information provided by this study identified the 10 best germplasm resources for fresh-eating and processing fruits.It can provide theoretical reference for nutritional efficacy evaluation and food development of A.arguta.
基金This work was supported by Core Premium Kiwifruit funding.
文摘Exogenous application of a cytokinin-like compound forchlorfenuron(CPPU)can promote fruit growth,although often at the expense of dry matter(DM),an important indicator of fruit quality.Actinidia chinensis var.deliciosa‘Hayward’fruit are very responsive to CPPU treatments,but the mechanism underlying the significant fruit weight increase and associated decrease in DM is unclear.In this study,we hypothesised that CPPU-enhanced growth increases fruit carbohydrate demand,but limited carbohydrate supply resulted in decreased fruit DM.During fruit development,CPPU effects on physical parameters,metabolites,osmotic pressure and transcriptional changes were assessed under conditions of both standard and a high carbohydrate supply.We showed that CPPU increased fruit fresh weight but the dramatic DM decrease was not carbohydrate limited.Enhanced glucose and fructose concentrations contributed to an increase in soluble carbohydrate osmotic pressure,which was correlated with increased water accumulation in CPPU-treated fruit and up-regulation of water channel aquaporin gene PIP2.4 at 49 days after anthesis.Transcipt analysis suggested that the molecular mechanism contributing to increased glucose and fructose concentrations was altered by carbohydrate supply.At standard carbohydrate supply,the early glucose increase in CPPU fruit was associated with reduced starch synthesis and increased starch degradation.When carbohydrate supply was high,the early glucose increase in CPPU fruit was associated with a general decrease in starch synthesis but up-regulation of vacuolar invertase and fructokinase genes.We conclude that CPPU affected fruit expansion by increasing the osmotically-driven water uptake and its effect was not carbohydrate supply-limited.
文摘Our study was conducted to determine In vitro germination ability of pollens from 25 wild Actinidia eriantha genotypes after one year freeze storage, afterwards we examined fertilization ability of stored pollens of 8 genotypes selected according to In vitro test results from aforementioned 25 genotypes, and finally investigated effects of stored viable pollens from “MH67”, “MH55”, “MH48” and “MH45” genotypes on fruit quality characters of the female experimental plant “M3” as the main purpose. Non-pollinated “M3” kiwifruit plant was taken as control. We found that In vitro germination and fertilization ability of different stored pollen sources, and also fruit quality characters including dry matter, total sugar, titratable acid, vitamin C, total polyphenol, total flavonoid, chlorophyll (“a”, “b”, and total) and carotenoid contents except total soluble solid content were significantly different. MH67 and MH45 genotypes were evaluated as the most suitable pollenizers which can be recommended as new candidate cultivars because of long term storage ability and brought about desired fruit quality characters. They also will be investigated more at further breeding studies.