The germination capacity of Ailanthus altissima seeds improved after the seeds were soaked with different concentrations of natural brassinolide （NBR）. The germination rate and germination energy of the seeds increa...The germination capacity of Ailanthus altissima seeds improved after the seeds were soaked with different concentrations of natural brassinolide （NBR）. The germination rate and germination energy of the seeds increased by 17.6% and 18.8%, and the mean germination speed （i.e., germination time） of the seeds was shortened by 1.4 d under the optimal concentration （0.4 mg·L^-1） treatment, compared with the control. After hypocotyls of A.altissima were treated with NBR, the elongation of the hypocotyls increased. Among different concentrations of the NBR, 0.4 mg·L^-1 NBR appeared to be the optimal concentration for the elongation of A.altissima hypocotyls.展开更多
Ailanthus altissima(Mill.)Swingle is a deciduous tree belonging to the Simaroubaceae family.The root and stem bark are traditional medicines for treating many diseases,such as diarrhea,dreaminess and intestinal tract ...Ailanthus altissima(Mill.)Swingle is a deciduous tree belonging to the Simaroubaceae family.The root and stem bark are traditional medicines for treating many diseases,such as diarrhea,dreaminess and intestinal tract bleeding.Phytochemical investigation of Ailanthus altissima(Mill.)Swingle led to the isolation of eight compounds.Chemical structures of these compounds were identified by analyzing their NMR spectroscopic data.Among them,compounds 1,4,5,6 and 8 were isolated from Ailanthus Desf.for the first time.The antioxidant activities of these compounds were determined by DPPH radical scavenging method.展开更多
Eight compounds(1-8)were isolated from Ailanthus altissima(Mill.)Swingle,including four lignans,three phenylpropanoids and a simple aromatic compound,their structures were identified by nuclear magnetic resonance spec...Eight compounds(1-8)were isolated from Ailanthus altissima(Mill.)Swingle,including four lignans,three phenylpropanoids and a simple aromatic compound,their structures were identified by nuclear magnetic resonance spectra and mass spectrometry data.Among them,compounds 1,3,5 and 8 were first obtained from this genus.In addition,free radical scavenging ability of compounds 1-8 were evaluated using DPPH,ABTS,and FRAP assays and the results showed that compound 2 exhibited potent antioxidant activity in DPPH and ABTS radical scavenging assays and compound 7 showed the highest value of FRAP radical scavenging.展开更多
Main observation and conclusion Quassinoids,a class of highly oxygenated triterpenes,have been isolated as bitter principles from the plants of Simaroubaceae family.Five new quassinoids,chouchunlactone A-E(1-5),and tw...Main observation and conclusion Quassinoids,a class of highly oxygenated triterpenes,have been isolated as bitter principles from the plants of Simaroubaceae family.Five new quassinoids,chouchunlactone A-E(1-5),and two known ones were identified from the root barks of Ailanthus altissima Swingle(Simaroubaceae).The chemical structures of the new compounds were deduced by spectroscopic data analyses,NMR calculations and DP4+probability analysis.The absolute configurations were defined by comparison of their experimental and calculated electronic circular dichroism(ECD)spectra data,and compounds 1-2 were further confirmed by single-crystal X-ray diffraction.Biologically,compound 5 showed potencies equivalent to sorafenib(IC50 value,9.70μmol·L–1)against HepG2 cells.The Hoechst 33342 staining,JC-1 fluorescent dye and Annexin V/PI analysis studies demonstrated that 5 can induce apoptosis and attenuate mitochondrial membrane potential(MMP)in HepG2 cells.展开更多
文摘The germination capacity of Ailanthus altissima seeds improved after the seeds were soaked with different concentrations of natural brassinolide （NBR）. The germination rate and germination energy of the seeds increased by 17.6% and 18.8%, and the mean germination speed （i.e., germination time） of the seeds was shortened by 1.4 d under the optimal concentration （0.4 mg·L^-1） treatment, compared with the control. After hypocotyls of A.altissima were treated with NBR, the elongation of the hypocotyls increased. Among different concentrations of the NBR, 0.4 mg·L^-1 NBR appeared to be the optimal concentration for the elongation of A.altissima hypocotyls.
文摘Ailanthus altissima(Mill.)Swingle is a deciduous tree belonging to the Simaroubaceae family.The root and stem bark are traditional medicines for treating many diseases,such as diarrhea,dreaminess and intestinal tract bleeding.Phytochemical investigation of Ailanthus altissima(Mill.)Swingle led to the isolation of eight compounds.Chemical structures of these compounds were identified by analyzing their NMR spectroscopic data.Among them,compounds 1,4,5,6 and 8 were isolated from Ailanthus Desf.for the first time.The antioxidant activities of these compounds were determined by DPPH radical scavenging method.
文摘Eight compounds(1-8)were isolated from Ailanthus altissima(Mill.)Swingle,including four lignans,three phenylpropanoids and a simple aromatic compound,their structures were identified by nuclear magnetic resonance spectra and mass spectrometry data.Among them,compounds 1,3,5 and 8 were first obtained from this genus.In addition,free radical scavenging ability of compounds 1-8 were evaluated using DPPH,ABTS,and FRAP assays and the results showed that compound 2 exhibited potent antioxidant activity in DPPH and ABTS radical scavenging assays and compound 7 showed the highest value of FRAP radical scavenging.
基金This work was financially supported by the National Natural Science Foundation of China(No.81872767)the Career Development Support Plan for the Young and Middle-aged Teachers in Shenyang Pharmaceutical University(No.ZQN2018006)+1 种基金the Pro ject funded by China Postdoctoral Science Foundation(No.2020M680987)the Project of Innovation Team(No.LT2015027)of Liaoning in China.
文摘Main observation and conclusion Quassinoids,a class of highly oxygenated triterpenes,have been isolated as bitter principles from the plants of Simaroubaceae family.Five new quassinoids,chouchunlactone A-E(1-5),and two known ones were identified from the root barks of Ailanthus altissima Swingle(Simaroubaceae).The chemical structures of the new compounds were deduced by spectroscopic data analyses,NMR calculations and DP4+probability analysis.The absolute configurations were defined by comparison of their experimental and calculated electronic circular dichroism(ECD)spectra data,and compounds 1-2 were further confirmed by single-crystal X-ray diffraction.Biologically,compound 5 showed potencies equivalent to sorafenib(IC50 value,9.70μmol·L–1)against HepG2 cells.The Hoechst 33342 staining,JC-1 fluorescent dye and Annexin V/PI analysis studies demonstrated that 5 can induce apoptosis and attenuate mitochondrial membrane potential(MMP)in HepG2 cells.
