目的:分析肾脏损伤因子-1(KIM-1)、胱抑素C(Cys-C)及β_(2)-微球蛋白(β_(2)-MG)的检测对慢性阻塞性肺疾病急性加重期(AECOPD)患者早期肾功能损伤的评估价值及临床意义。方法:选定南京市浦口区中心医院呼吸内科确诊的300例AECOPD患者,...目的:分析肾脏损伤因子-1(KIM-1)、胱抑素C(Cys-C)及β_(2)-微球蛋白(β_(2)-MG)的检测对慢性阻塞性肺疾病急性加重期(AECOPD)患者早期肾功能损伤的评估价值及临床意义。方法:选定南京市浦口区中心医院呼吸内科确诊的300例AECOPD患者,将其设为AECOPD组,另选取同期浦口区中心医院门诊体检中心100例健康体检者,将其设为健康组。根据血气分析氧分压(PaO_(2))检测结果将AECOPD患者分为三组,分别是Ⅰ级组(60 mm Hg<PaO_(2)≤80 mm Hg)、Ⅱ级组(40 mm Hg<PaO_(2)≤60 mm Hg)、Ⅲ级组(PaO_(2)≤40 mm Hg)。检测并比较AECOPD组、健康组尿KIM-1、血清β_(2)-MG、Cys-C、肌酐(SCr)、尿素氮(BUN)。根据肾小球滤过率(eGFR)将AECOPD患者分为两组,eGFR≥90 ml/(min·1.73 m^(2))的120例患者为肾功能正常组,eGFR<90 ml/(min·1.73 m^(2))的180例患者为早期肾功能损伤,比较两组尿KIM-1、血清β_(2)-MG、Cys-C、SCr、BUN,Pearson相关性分析KIM-1、β_(2)-MG、Cys-C与SCr、BUN的相关性。结果:Ⅲ级组尿KIM-1、血清β_(2)-MG、Cys-C、SCr、BUN均高于Ⅱ级、Ⅰ级、健康组(P<0.05)。早期肾功能损伤组尿KIM-1、血清β_(2)-MG、Cys-C、SCr、BUN均高于肾功能正常组(P<0.05)。KIM-1、β_(2)-MG、Cys-C与SCr、BUN均呈正相关性(P<0.05)。结论:AECOPD早期肾功能损伤患者尿KIM-1、血清β_(2)-MG、Cys-C水平均较高,与患者肾功能受损程度联系密切,具有一定的参考价值。展开更多
α_(s1)-Casein is a potential allergen to induce hypersensitivity in cow milk.We had identifiedα_(s1)-casein and its epitopes in previous studies.The present study aimed to evaluate the allergic mechanism ofα_(s1)-c...α_(s1)-Casein is a potential allergen to induce hypersensitivity in cow milk.We had identifiedα_(s1)-casein and its epitopes in previous studies.The present study aimed to evaluate the allergic mechanism ofα_(s1)-casein in a BALB/c mouse model.The levels of specific IgE,mast cell proteinase,histamine and cytokines in sensitized mice were determined,and the clinical and pathological observation were evaluated.Results showed that the levels of specific IgE,mast cell proteinase,histamine,IL-4,IL-5,IL-10 increased significantly with a dose-dependent trend.The local alveolar septum collapsed or thickened,and lymphatic foci were produced in the spleen and thymus,and the inflammatory cells infiltrated in small intestinal mucosa mesenchyme.In conclusion,the levels of specific IgE,mast cell proteinase,histamine,IL-4,IL-5,IL-10 and some inflammatory factors could possibly serve as allergic biomarkers ofα_(s1)-casein,however,additional studies on signal transduction and gene expression are necessary in future.展开更多
文摘目的:分析肾脏损伤因子-1(KIM-1)、胱抑素C(Cys-C)及β_(2)-微球蛋白(β_(2)-MG)的检测对慢性阻塞性肺疾病急性加重期(AECOPD)患者早期肾功能损伤的评估价值及临床意义。方法:选定南京市浦口区中心医院呼吸内科确诊的300例AECOPD患者,将其设为AECOPD组,另选取同期浦口区中心医院门诊体检中心100例健康体检者,将其设为健康组。根据血气分析氧分压(PaO_(2))检测结果将AECOPD患者分为三组,分别是Ⅰ级组(60 mm Hg<PaO_(2)≤80 mm Hg)、Ⅱ级组(40 mm Hg<PaO_(2)≤60 mm Hg)、Ⅲ级组(PaO_(2)≤40 mm Hg)。检测并比较AECOPD组、健康组尿KIM-1、血清β_(2)-MG、Cys-C、肌酐(SCr)、尿素氮(BUN)。根据肾小球滤过率(eGFR)将AECOPD患者分为两组,eGFR≥90 ml/(min·1.73 m^(2))的120例患者为肾功能正常组,eGFR<90 ml/(min·1.73 m^(2))的180例患者为早期肾功能损伤,比较两组尿KIM-1、血清β_(2)-MG、Cys-C、SCr、BUN,Pearson相关性分析KIM-1、β_(2)-MG、Cys-C与SCr、BUN的相关性。结果:Ⅲ级组尿KIM-1、血清β_(2)-MG、Cys-C、SCr、BUN均高于Ⅱ级、Ⅰ级、健康组(P<0.05)。早期肾功能损伤组尿KIM-1、血清β_(2)-MG、Cys-C、SCr、BUN均高于肾功能正常组(P<0.05)。KIM-1、β_(2)-MG、Cys-C与SCr、BUN均呈正相关性(P<0.05)。结论:AECOPD早期肾功能损伤患者尿KIM-1、血清β_(2)-MG、Cys-C水平均较高,与患者肾功能受损程度联系密切,具有一定的参考价值。
基金supported by National Science and Technology Project in Rural Areas(China,2018YFC1604205)the National Natural Science Foundation of China(Beijing,China31872886)。
文摘α_(s1)-Casein is a potential allergen to induce hypersensitivity in cow milk.We had identifiedα_(s1)-casein and its epitopes in previous studies.The present study aimed to evaluate the allergic mechanism ofα_(s1)-casein in a BALB/c mouse model.The levels of specific IgE,mast cell proteinase,histamine and cytokines in sensitized mice were determined,and the clinical and pathological observation were evaluated.Results showed that the levels of specific IgE,mast cell proteinase,histamine,IL-4,IL-5,IL-10 increased significantly with a dose-dependent trend.The local alveolar septum collapsed or thickened,and lymphatic foci were produced in the spleen and thymus,and the inflammatory cells infiltrated in small intestinal mucosa mesenchyme.In conclusion,the levels of specific IgE,mast cell proteinase,histamine,IL-4,IL-5,IL-10 and some inflammatory factors could possibly serve as allergic biomarkers ofα_(s1)-casein,however,additional studies on signal transduction and gene expression are necessary in future.
