The isopiestic method was used to determinate isopiestic molality and osmotic coefficients in single salt solutions of LiCl H 2O(molality range from 0.5 to 9.0 mol·kg -1 ),CaCl 2 H 2O(molality range from 0.35 to ...The isopiestic method was used to determinate isopiestic molality and osmotic coefficients in single salt solutions of LiCl H 2O(molality range from 0.5 to 9.0 mol·kg -1 ),CaCl 2 H 2O(molality range from 0.35 to 5.2 mol·kg -1 ) and multisalt solution LiCl CaCl 2 H 2O(ionic strength range from 0.5 to 15.7 mol·kg -1 ) at 298.15K,from which Pitzer’s interaction parameters of single salt ,mixed salts of the systems were obtained.The mean ionic activity coefficients of LiCl、CaCl 2 in pure and mixed solutions were reported.展开更多
Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ ...Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3,and 0 05?mmol·L -1 of CaM antagonist CPZ. The changes in the contents of total soluble protein and CaM, and freezing resistance in all pretreated seedlings in various periods ( viz: following cold acclimation, chilling stress and recovery) were investigated. Results showed that cold acclimation increased the contents of total soluble protein and CaM, and freezing resistance of seedlings, which could be strongly reduced by the pretreatments of EGTA CPZ and LaCl 3 Cold acclimation combined with CaCl 2 pretreatment enhanced the effect of cold acclimation on freezing resistance, and obviously increased the contents of total soluble protein and CaM, reduced the declining degree of the contents of total soluble protein and CaM caused by chilling stress as compared with cold acclimation, augmented the increase in the level of total soluble protein and CaM during the recovery periods. Further analysis found that an increase in total soluble protein content during cold acclimation with or without CaCl 2 pretreatment mainly resulted from the increase in content of heat stable protein in total soluble protein. It is suggested that Ca 2+ calmodulin may be involved in the synthesis of total soluble protein, and the induction of freezing resistance of seedlings.展开更多
Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=...Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=532 nm/685 nm. The enhancement effect of ⅡA element ions(such as Ca 2+, Sr 2+ and Ba 2+) on RTP of eosin-ITC is much obvious than that of Pb 2+ and Hg 2+ used constantly as an outer heavy atom perturber in phosphorimetry. Based on that, a new solid substrate room temperature phosphorescence immunoassay(SS-RTP-IA) for the determination of the human IgG was proposed to demonstrate the feasibility using eosin-ITC as phosphorescent marker. It was found that a good linear relationship with the ΔI P was obtained when the amount of human IgG in the range from 6.25 to 400 pg/spot(corresponding to the mass concentration: 15.63 ng/mL—1 μg/L, sample volume of 0.4 μL/spot). The regression {equation} of working curve can be expressed as ΔI p=3.062+0.00864x(pg)(n=7, r=0.996), The detection limit calculated as 3S b/k is 0.394 pg/spot. The method was applied directly to the determination of IgG in human serum with a satisfying result. These results show that eosin-ITC can be used conveniently as a RTP marker of high sensitivity.展开更多
文摘The isopiestic method was used to determinate isopiestic molality and osmotic coefficients in single salt solutions of LiCl H 2O(molality range from 0.5 to 9.0 mol·kg -1 ),CaCl 2 H 2O(molality range from 0.35 to 5.2 mol·kg -1 ) and multisalt solution LiCl CaCl 2 H 2O(ionic strength range from 0.5 to 15.7 mol·kg -1 ) at 298.15K,from which Pitzer’s interaction parameters of single salt ,mixed salts of the systems were obtained.The mean ionic activity coefficients of LiCl、CaCl 2 in pure and mixed solutions were reported.
文摘Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3,and 0 05?mmol·L -1 of CaM antagonist CPZ. The changes in the contents of total soluble protein and CaM, and freezing resistance in all pretreated seedlings in various periods ( viz: following cold acclimation, chilling stress and recovery) were investigated. Results showed that cold acclimation increased the contents of total soluble protein and CaM, and freezing resistance of seedlings, which could be strongly reduced by the pretreatments of EGTA CPZ and LaCl 3 Cold acclimation combined with CaCl 2 pretreatment enhanced the effect of cold acclimation on freezing resistance, and obviously increased the contents of total soluble protein and CaM, reduced the declining degree of the contents of total soluble protein and CaM caused by chilling stress as compared with cold acclimation, augmented the increase in the level of total soluble protein and CaM during the recovery periods. Further analysis found that an increase in total soluble protein content during cold acclimation with or without CaCl 2 pretreatment mainly resulted from the increase in content of heat stable protein in total soluble protein. It is suggested that Ca 2+ calmodulin may be involved in the synthesis of total soluble protein, and the induction of freezing resistance of seedlings.
文摘Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=532 nm/685 nm. The enhancement effect of ⅡA element ions(such as Ca 2+, Sr 2+ and Ba 2+) on RTP of eosin-ITC is much obvious than that of Pb 2+ and Hg 2+ used constantly as an outer heavy atom perturber in phosphorimetry. Based on that, a new solid substrate room temperature phosphorescence immunoassay(SS-RTP-IA) for the determination of the human IgG was proposed to demonstrate the feasibility using eosin-ITC as phosphorescent marker. It was found that a good linear relationship with the ΔI P was obtained when the amount of human IgG in the range from 6.25 to 400 pg/spot(corresponding to the mass concentration: 15.63 ng/mL—1 μg/L, sample volume of 0.4 μL/spot). The regression {equation} of working curve can be expressed as ΔI p=3.062+0.00864x(pg)(n=7, r=0.996), The detection limit calculated as 3S b/k is 0.394 pg/spot. The method was applied directly to the determination of IgG in human serum with a satisfying result. These results show that eosin-ITC can be used conveniently as a RTP marker of high sensitivity.
