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Current status and challenges for cell-cultured milk technology: a systematic review
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作者 Hyuk Cheol Kwon Hyun Su Jung +1 位作者 Vahinika Kothuri Sung Gu Han 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第5期1778-1792,共15页
Cellular agriculture is an innovative technology for manufacturing sustainable agricultural products as an alternative to traditional agriculture.While most cellular agriculture is predominantly centered on the produc... Cellular agriculture is an innovative technology for manufacturing sustainable agricultural products as an alternative to traditional agriculture.While most cellular agriculture is predominantly centered on the production of cultured meat,there is a growing demand for an understanding of the production techniques involved in dairy products within cellular agriculture.This review focuses on the current status of cellular agriculture in the dairy sector and technical challenges for cell-cultured milk production.Cellular agriculture technology in the dairy sector has been classified into fermentation-based and animal cell culture-based cellular agriculture.Currently,various companies synthesize milk components through precision fermentation technology.Nevertheless,several startup companies are pursuing animal cell-based technology,driven by public concerns regarding genetically modified organisms in precision fermentation technology.Hence,this review offers an up-to-date exploration of animal cell-based cellular agriculture to produce milk components,specifically emphasizing the structural,functional,and productive aspects of mammary epithelial cells,providing new information for industry and academia. 展开更多
关键词 cell culture system cell-cultured milk Mammary epithelial cells Precision fermentation
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MACS-W:A modified optical clearing agent for imaging 3D cell cultures
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作者 Xiang Zhong Chao Gao +6 位作者 Hui Li Yuening He Peng Fei Zaozao Chen Zhongze Gu Dan Zhu Tingting Yu 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2024年第2期24-34,共11页
Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible... Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures. 展开更多
关键词 Tissue optical clearing 3D cell cultures IMAGING
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3D Collagen Gels:A Promising Platform for Dendritic Cell Culture in Biomaterials Research
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作者 Kirubanandan Shanmugam 《Proceedings of Anticancer Research》 2024年第4期124-134,共11页
The three-dimensional(3D)cell culture system has garnered significant attention in recent years as a means of studying cell behavior and tissue development,as opposed to traditional two-dimensional cultures.These syst... The three-dimensional(3D)cell culture system has garnered significant attention in recent years as a means of studying cell behavior and tissue development,as opposed to traditional two-dimensional cultures.These systems can induce specific cell reactions,promote specific tissue functions,and serve as valuable tools for research in tissue engineering,regenerative medicine,and drug discovery.This paper discusses current developments in the field of three-dimensional cell culture and the potential applications of 3D type 1 collagen gels to enhance the growth and maturation of dendritic cells. 展开更多
关键词 Three-dimensional cell culture Dendritic cells Type 1 collagen gels Bovine tendons and rat tails
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Optimization of Culture Medium and Transfection Method for Head and Neck Squamous Cell Carcinoma Organoids
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作者 Zhongheng HUANG Xi YAO +2 位作者 Qi LIU Ying XIE Zhengbo WEI 《Medicinal Plant》 CAS 2023年第3期100-104,共5页
[Objectives] To optimize the culture medium for head and neck squamous cell carcinoma patient-derived organoid and screen suitable cytokines;compare the transfection efficiency of direct transfection and short-term su... [Objectives] To optimize the culture medium for head and neck squamous cell carcinoma patient-derived organoid and screen suitable cytokines;compare the transfection efficiency of direct transfection and short-term suspension transfection for organoid in matrigel. [Methods] Advanced DMEM/F12 medium, GlutaMax and HEPES buffer, nicotinamide, N-acetylcysteine, B27, A83-01, EGF, Y-27632 and Primocin primary cell antibiotics were prepared. On this basis, fibroblast growth factor 10(FGF10), Neuregulin 1, Noggin and R-spondin-1 were added in turn to prepare the selection medium, and the organoid diameter was used as the evaluation index to evaluate the effect of organoid medium. Using lentivirus, mCherry red fluorescent protein was transfected into HNSCC—PDO in different ways, and the transfection effect was evaluated by the fluorescence intensity of organoid sphere. [Results] Nrg1 Noggin and R-Spondin-1 promoted the growth of head and neck squamous cell carcinoma sphere(P<0.05) while FGF10 did not significantly promote the growth of head and neck squamous cell carcinoma sphere(P>0.05). Compared with direct transfection, short-term suspension transfection had higher transfection efficiency for HNSCC—PDO in matrigel. [Conclusions] R-Spondin-1 Nrg1 and Noggin may be the key cytokines in culture of HNSCC—PDO whereas FGF10 played an insignificant role in this study. Short-term suspension transfection could improve the transfection efficiency of lentivirus to HNSCC—PDO. 展开更多
关键词 Head and neck squamous cell carcinoma Organoid culture Organoid transfection
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Differentiation potential of human adipose tissue derived stem cells into photoreceptors through explants culture and enzyme methods 被引量:3
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作者 Wei-Wei Xu Li Huang +5 位作者 Kelvin K.L.Chong Doreen S.Y.Leung Benjamin EL.Li Zheng-Qin Yin Yi-Fei Huang Chi Pui Pang 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2017年第1期23-29,共7页
AIM: To investigate the retinal photoreceptor differentiation potential of human orbital adipose tissue-derived stem cells (ADSCs) generated by enzyme (EN) and explant (EX) culture methods.METHODS: We investig... AIM: To investigate the retinal photoreceptor differentiation potential of human orbital adipose tissue-derived stem cells (ADSCs) generated by enzyme (EN) and explant (EX) culture methods.METHODS: We investigated potentials of human orbital ADSCs to differentiate into photoreceptors through EN and EX culture methods. EN and EX orbital ADSCs were obtained from the same donor during rehabilitative orbital decompression, and then were subject to a 3-step induction using Noggin, DKK-1, IGF-1 and b-FGF at different time points for 38d. Stem cell, eye-field and photoreceptor-related gene and protein markers were measured by reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescent (IMF) staining.RESULTS: Both EX and EN orbital ADSCs expressed CD133, a marker of cell differentiation. Moreover, PAX6 and rhodopsin, markers of the retinal progenitor cells, were detected from EX and EN orbital ADSCs. In EX orbital ADSCs, PAX6 mRNA was detected on the 17th day and then the rhodopsin mRNA was detected on the 24th day. In contrast, the EN orbital ADSCs expressed PAX6 and rhodopsin mRNA on the 31st day. EX orbital ADSCs expressed rhodopsin protein on the 24th day, while EN orbital ADSCs expressed rhodopsin protein on the 31st day. CONCLUSION: Orbital ADSCs isolated by direct explants culture show earlier and stronger expressions of markers towards eye field and retinal photoreceptor differentiation than those generated by conventional EN method. 展开更多
关键词 photoreceptor cells cell differentiation adultstem cells tissue engineering explants culture enzymaticdigestion
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Comparison of two methods used to culture and purify rat retinal Mller cells 被引量:2
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作者 Wei-Tao Song Xue-Yong Zhang +3 位作者 Si-Qi Xiong Dan Wen Jian Jiang Xiao-Bo Xia 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2013年第6期778-784,共7页
AIM:To study two methods for culturing and purifying Sprague-Dawley(SD)rat retinal Muller cells and determine which one is better.METHODS:The passage culture method of Muller cells was respectively carried out by comp... AIM:To study two methods for culturing and purifying Sprague-Dawley(SD)rat retinal Muller cells and determine which one is better.METHODS:The passage culture method of Muller cells was respectively carried out by complete pancreatic enzyme digestion method and repeated incomplete pancreatic enzyme digestion method.After culturing retinal cells for one month through these two methods,fluorescence-activated cell sorter(FACS),RT-PCR,and immunohistochemistry technology were performed to examine the enrichment and purity of Muller glial cells,and carried out two-sample approximate t test using SSPS 13.0 to further compare the Muller cell positive rate in both methods.RESULTS:The statistical results showed that the purity of Muller cells was 83.2%±5.16%in group A,and the purity was 98.5%±1.08%in group B.The two-sample approximate t test analysis demonstrated that the difference between group A and group B was statistically significant(t=-9.178,P【0.005).The results clearly exhibited a difference between the purity of Muller cells cultured by the complete pancreatic enzyme digestion method(group A)and the repeated incomplete pancreatic enzyme digestion method(group B).CONCLUSION:Compared with the complete pancreatic enzyme digestion method,this novel method was more efficient and a higher purity of Muller cells could be obtained using this approach. 展开更多
关键词 primary culture PASSAGE PURIFICATION retinal Mller cell trypsinization
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Simplified methods to isolate,culture and purify olfactory ensheathing cells 被引量:1
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作者 Zhengfeng Lu Yixin Shen +3 位作者 Peng Zhang Zhihai Fan Qirong Dong Min Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第19期1495-1499,共5页
Conventional methods for harvesting, culturing and purifying olfactory ensheathing cells are complicated, time-consuming, and poorly reproducible. Olfactory bulbs were detached from adult Sprague Dawley rats and olfac... Conventional methods for harvesting, culturing and purifying olfactory ensheathing cells are complicated, time-consuming, and poorly reproducible. Olfactory bulbs were detached from adult Sprague Dawley rats and olfactory ensheathing cells were isolated using shearing, dispersion processes. After the primary cultures reached confluence, the cells were purified using a three-step process. The olfactory ensheathing cells attached and grew rapidly. The purity of the olfactory ensheathing cells increased following the three purification steps, eventually exceeding 95%. These cells could be maintained for an extended period time in culture. This simple, inexpensive, reproducible method of harvesting, culturing and purifying olfactory ensheathing cells shortens the culture cycle and provides sufficient olfactory ensheathing cells of controllable purity. 展开更多
关键词 olfactory ensheathing cells SHEARING ISOLATION primary culture PURIFICATION in vitro olfactory bulb rats
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Comparison on In-Vitro Culture Methods of Yak Endometrial Gland Epithelial Cells
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作者 WU Qing-xia DONG Hal-long RUI Ya-pei 《Animal Husbandry and Feed Science》 CAS 2011年第4期9-12,共4页
[ Objective] To develop in-vitro culture methods of yak endometrial gland epithelial cells. [Method] The gland epithelial cells were isolated from yak endometrium by explant culture method and digestion culture method... [ Objective] To develop in-vitro culture methods of yak endometrial gland epithelial cells. [Method] The gland epithelial cells were isolated from yak endometrium by explant culture method and digestion culture method, respectively. [ Result] In the first method, the cells isolated from the endometrium explant could merge into monolayer after 8-d culture, and they could be purified by gradation digestion with trypsin. In the second method, the endometrium explant were first digested by collagenase II by incubation at 37℃ for 2.5 h and then further digested in fresh 2 g/L colla- genase II for another 2.5 h. The cell suspension was leached through 74 pm filter and centrifuged at 400 r/min for 5 min. Then the cell pellet was re-suspended, followed by natural sedimentation to collect purified gland epithelial cells. The isolated cells were cytokeratin-positive as detected by immunocytochemical staining, and the positive rate could reach 95%. [Conclusion] The yak endometrial gland epithelial cells can be isolated and purified by both the explant culture method and digestion culture method. 展开更多
关键词 YAK ENDOMETRIUM Gland epithelial cells In-vitro culture
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Sensitivity of diagnosis of spontaneous bacterial peritonitis is higher with the automated cell count method
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作者 Juan G Acevedo-Haro Waddah Mohamed +8 位作者 Prebashan Moodley Oliver Bendall Kris Bennett Nigel Keelty Sally Chan Sam Waddy Joanne Hosking Wayne Thomas Robert Tilley 《World Journal of Hepatology》 2024年第11期1265-1281,共17页
BACKGROUND Spontaneous bacterial peritonitis(SBP)is one of the most important complications of patients with liver cirrhosis entailing high morbidity and mortality.Making an accurate early diagnosis of this infection ... BACKGROUND Spontaneous bacterial peritonitis(SBP)is one of the most important complications of patients with liver cirrhosis entailing high morbidity and mortality.Making an accurate early diagnosis of this infection is key in the outcome of these patients.The current definition of SBP is based on studies performed more than 40 years ago using a manual technique to count the number of polymorphs in ascitic fluid(AF).There is a lack of data comparing the traditional cell count method with a current automated cell counter.Moreover,current international guidelines do not mention the type of cell count method to be employed and around half of the centers still rely on the traditional manual method.AIM To compare the accuracy of polymorph count on AF to diagnose SBP between the traditional manual cell count method and a modern automated cell counter against SBP cases fulfilling gold standard criteria:Positive AF culture and signs/symptoms of peritonitis.METHODS Retrospective analysis including two cohorts:Cross-sectional(cohort 1)and case-control(cohort 2),of patients with decompensated cirrhosis and ascites.Both cell count methods were conducted simultaneously.Positive SBP cases had a pathogenic bacteria isolated on AF and signs/symptoms of peritonitis.RESULTS A total of 137 cases with 5 positive-SBP,and 85 cases with 33 positive-SBP were included in cohort 1 and 2,respectively.Positive-SBP cases had worse liver function in both cohorts.The automated method showed higher sensitivity than the manual cell count:80%vs 52%,P=0.02,in cohort 2.Both methods showed very good specificity(>95%).The best cutoff using the automated cell counter was polymorph≥0.2 cells×10^(9)/L(equivalent to 200 cells/mm^(3))in AF as it has the higher sensitivity keeping a good specificity.CONCLUSION The automated cell count method should be preferred over the manual method to diagnose SBP because of its higher sensitivity.SBP definition,using the automated method,as polymorph cell count≥0.2 cells×10^(9)/L in AF would need to be considered in patients admitted with decompensated cirrhosis. 展开更多
关键词 Spontaneous bacterial peritonitis DIAGNOSIS CIRRHOSIS Bacterial infection Automated cell count method Manual cell count method Ascitic fluid
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Culture and identification of neonatal rat brain-derived neural stem cells
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作者 Qing-Zhong Zhou Xiao-Lan Feng +4 位作者 Xu-Feng Jia Nurul Huda Binti Mohd Nor Mohd Hezery Bin Harun Da-Xiong Feng Wan Aliaa Wan Sulaiman 《World Journal of Stem Cells》 SCIE 2023年第6期607-615,共9页
BACKGROUND Timing of passaging,passage number,passaging approaches and methods for cell identification are critical factors influencing the quality of neural stem cells(NSCs)culture.How to effectively culture and iden... BACKGROUND Timing of passaging,passage number,passaging approaches and methods for cell identification are critical factors influencing the quality of neural stem cells(NSCs)culture.How to effectively culture and identify NSCs is a continuous interest in NSCs study while these factors are comprehensively considered.AIM To establish a simplified and efficient method for culture and identification of neonatal rat brain-derived NSCs.METHODS First,curved tip operating scissors were used to dissect brain tissues from new born rats(2 to 3 d)and the brain tissues were cut into approximately 1 mm^(3)sections.Filter the single cell suspension through a nylon mesh(200-mesh)and culture the sections in suspensions.Passaging was conducted with TrypLTM Express combined with mechanical tapping and pipetting techniques.Second,identify the 5th generation of passaged NSCs as well as the revived NSCs from cryopreservation.BrdU incorporation method was used to detect self-renew and proliferation capabilities of cells.Different NSCs specific antibodies(anti-nestin,NF200,NSE and GFAP antibodies)were used to identify NSCs specific surface markers and muti-differentiation capabilities by immunofluorescence staining.RESULTS Brain derived cells from newborn rats(2 to 3 d)proliferate and aggregate into spherical-shaped clusters with sustained continuous and stable passaging.When BrdU was incorporated into the 5th generation of passaged cells,positive BrdU cells and nestin cells were observed by immunofluorescence staining.After induction of dissociation using 5%fetal bovine serum,positive NF200,NSE and GFAP cells were observed by immunofluorescence staining.CONCLUSION This is a simplified and efficient method for neonatal rat brain-derived neural stem cell culture and identification. 展开更多
关键词 Neonatal rats Brain-derived neural stem cells culture IDENTIFICATION
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An Innovative Design of Incubator Structure for Cell Culture
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作者 Shanshan HE Zhongwei CHEN +2 位作者 Ruonan HE Shiyi WU Qihuang LIN 《Medicinal Plant》 CAS 2023年第3期105-107,共3页
In view of the problems of the traditional cell incubator,such as the small range of cell culture types,the inability to adjust the internal space of the incubator according to needs,and the inconvenient sampling,this... In view of the problems of the traditional cell incubator,such as the small range of cell culture types,the inability to adjust the internal space of the incubator according to needs,and the inconvenient sampling,this study innovatively designed a cell incubator structure.It proposed a new design concept that can solve the above-mentioned shortcomings.The cell incubator after the new structural modification can adjust the internal space structure of cell culture by setting the bolt-fixed connection between the fixed plate and the vessel divider.It realizes the cultivation of various cells through refrigeration modules and heating modules.Through setting a sampling hole in the glass inner door,it is favorable for operators to take samples,making cell culture more convenient and efficient. 展开更多
关键词 cell incubator Innovative design cell culture
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Repetitive administration of cultured human CD34+cells improve adenine-induced kidney injury in mice
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作者 Takayasu Ohtake Shoichi Itaba +9 位作者 Amankeldi A Salybekov Yin Sheng Tsutomu Sato Mitsuru Yanai Makoto Imagawa Shigeo Fujii Hiroki Kumagai Masamitsu Harata Takayuki Asahara Shuzo Kobayashi 《World Journal of Stem Cells》 SCIE 2023年第4期268-280,共13页
BACKGROUND There is no established treatment to impede the progression or restore kidney function in human chronic kidney disease(CKD).AIM To examine the efficacy of cultured human CD34+cells with enhanced proliferati... BACKGROUND There is no established treatment to impede the progression or restore kidney function in human chronic kidney disease(CKD).AIM To examine the efficacy of cultured human CD34+cells with enhanced proliferating potential in kidney injury in mice.METHODS Human umbilical cord blood(UCB)-derived CD34+cells were incubated for one week in vasculogenic conditioning medium.Vasculogenic culture significantly increased the number of CD34+cells and their ability to form endothelial progenitor cell colony-forming units.Adenineinduced tubulointerstitial injury of the kidney was induced in immunodeficient non-obese diabetic/severe combined immunodeficiency mice,and cultured human UCB-CD34+cells were administered at a dose of 1×106/mouse on days 7,14,and 21 after the start of adenine diet.RESULTS Repetitive administration of cultured UCB-CD34+cells significantly improved the time-course of kidney dysfunction in the cell therapy group compared with that in the control group.Both interstitial fibrosis and tubular damage were significantly reduced in the cell therapy group compared with those in the control group(P<0.01).Microvasculature integrity was significantly preserved(P<0.01)and macrophage infiltration into kidney tissue was dramatically decreased in the cell therapy group compared with those in the control group(P<0.001).CONCLUSION Early intervention using human cultured CD34+cells significantly improved the progression of tubulointerstitial kidney injury.Repetitive administration of cultured human UCB-CD34+cells significantly improved tubulointerstitial damage in adenine-induced kidney injury in mice via vasculoprotective and anti-inflammatory effects. 展开更多
关键词 Chronic kidney disease CD34+cell ADENINE Tubulointerstitial injury Quality and quantity control culture Umbilical cord blood
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Sequential extraction of RNA,DNA and protein from cultured cells of the same group
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作者 Ying-Yu Cui 《World Journal of Methodology》 2023年第5期484-491,共8页
BACKGROUND Efficient extraction of nucleic acids and proteins(ENAP)from cells is a prerequisite for precise annotation of gene function,and has become laboratory routine for revealing the mysteries of life.However,cel... BACKGROUND Efficient extraction of nucleic acids and proteins(ENAP)from cells is a prerequisite for precise annotation of gene function,and has become laboratory routine for revealing the mysteries of life.However,cell samples are often from different culture dishes,resulting in inevitable experimental errors and sometimes poor repeatability.AIM To explore a method to improve the efficiency of ENAP,minimizing errors in ENAP processes,enhancing the reliability and repeatability of subsequent experimental results.METHODS A protocol for the sequential isolation of RNA,DNA,and proteins from the same cultured HepG2 cells using RNAzol reagent is presented here.The first step involves culturing HepG2 cells to the exponential phase,followed by the sequential isolation of RNA,DNA,and proteins from the same cultured cells in the second step.The yield of nucleic acids and proteins is detected in the third step,and their purity and integrity are verified in the last step.RESULTS The procedure takes as few as 3-4 d from the start to quality verification and is highly efficient.In contrast to the existing kits and reagents,which are primarily based on independent isolation,this RNAzol reagent-based method is characterized by the sequential isolation of RNA,DNA,and proteins from the same cells,and therefore saves time,and has low cost and high efficiency.CONCLUSION The RNA,DNA,and proteins isolated using this method can be used for reverse transcription-polymerase chain reaction,polymerase chain reaction,and western blotting,respectively. 展开更多
关键词 Sequential extraction Ribonucleic acid Deoxyribonucleic acid PROTEIN cultured cells
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A novel primary culture method for high-purity satellite glial cells derived from rat dorsal root ganglion 被引量:1
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作者 Xian-Bin Wang Wei Ma +5 位作者 Tao Luo Jin-Wei Yang Xiang-Peng Wang Yun-Fei Dai Jian-Hui Guo Li-Yan Li 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第2期339-345,共7页
Satellite glial cells surround neurons within dorsal root ganglia. Previous studies have focused on single-cell suspensions of cultured neurons derived from rat dorsal root ganglia. At present, the primary culture met... Satellite glial cells surround neurons within dorsal root ganglia. Previous studies have focused on single-cell suspensions of cultured neurons derived from rat dorsal root ganglia. At present, the primary culture method for satellite glial cells derived from rat dorsal root ganglia requires no digestion skill. Hence, the aim of the present study was to establish a novel primary culture method for satellite glial cells derived from dorsal root ganglia. Neonatal rat spine was collected and an incision made to expose the transverse protrusion and remove dorsal root ganglia. Dorsal root ganglia were freed from nerve fibers, connective tissue, and capsule membranes, then rinsed and transferred to 6-well plates, and cultured in a humidified 5% CO_2 incubator at 37°C. After 3 days in culture, some cells had migrated from dorsal root ganglia. After subculture, cells were identified by immunofluorescence labeling for three satellite glial cell-specific markers: glutamine synthetase, glial fibrillary acidic protein, and S100β. Cultured cells expressed glutamine synthetase, glial fibrillary acidic protein, and S100β, suggesting they are satellite glial cells with a purity of > 95%. Thus, we have successfully established a novel primary culture method for obtaining high-purity satellite glial cells from rat dorsal root ganglia without digestion. 展开更多
关键词 nerve REGENERATION cell culture dorsal root GANGLIA IMMUNOFLUORESCENCE identification SATELLITE GLIAL cells neural REGENERATION
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Effect of culture methods on individual variation in the growth of sea cucumber Apostichopus japonicus within a cohort and family 被引量:2
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作者 邱天龙 张立斌 +2 位作者 张涛 柏雨岑 杨红生 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2014年第4期737-742,共6页
There is substantial individual variation in the growth rates of sea cucumber Apostiehopus japonicus individuals. This necessitates additional work to grade the seed stock and lengthens the production period. We evalu... There is substantial individual variation in the growth rates of sea cucumber Apostiehopus japonicus individuals. This necessitates additional work to grade the seed stock and lengthens the production period. We evaluated the influence of three culture methods (free-mixed, isolated-mixed, isolated-alone) on individual variation in growth and assessed the relationship between feeding, energy conversion efficiency, and individual growth variation in individually cultured sea cucumbers. Of the different culture methods, animals grew best when reared in the isolated-mixed treatment (i.e., size classes were held separately), though there was no difference in individual variation in growth between rearing treatment groups. The individual variation in growth was primarily attributed to genetic factors. The difference in food conversion efficiency caused by genetic differences among individuals was thought to be the origin of the variance. The level of individual growth variation may be altered by interactions among individuals and environmental heterogeneity. Our results suggest that, in addition to traditional seed grading, design of a new kind of substrate that changes the spatial distribution of sea cucumbers would effectively enhance growth and reduce individual variation in growth of sea cucumbers in culture. 展开更多
关键词 Apostichopusjaponicas individual growth variation culture methods family sea cucumber
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Modeling One Dimensional Two-Cell Model with Tumor Interaction Using Krylov Subspace Methods
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作者 Ibtisam Alqahtani Sharefa Eisa Ali Alhazmi 《Applied Mathematics》 2023年第1期21-34,共14页
A brain tumor occurs when abnormal cells grow, sometimes very rapidly, into an abnormal mass of tissue. The tumor can infect normal tissue, so there is an interaction between healthy and infected cell. The aim of this... A brain tumor occurs when abnormal cells grow, sometimes very rapidly, into an abnormal mass of tissue. The tumor can infect normal tissue, so there is an interaction between healthy and infected cell. The aim of this paper is to propose some efficient and accurate numerical methods for the computational solution of one-dimensional continuous basic models for the growth and control of brain tumors. After computing the analytical solution, we construct approximations of the solution to the problem using a standard second order finite difference method for space discretization and the Crank-Nicolson method for time discretization. Then, we investigate the convergence behavior of Conjugate gradient and generalized minimum residual as Krylov subspace methods to solve the tridiagonal toeplitz matrix system derived. 展开更多
关键词 PDES Krylov Subspace methods Finite Difference Toeplitz Matrix Two-cell Model Tumor Interaction Modeling
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EVALUATION OF BIOCOMPATIBILITY OF MATERIALS BY A CELL CULTURE METHOD
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作者 张彩霞 今井庸二 +1 位作者 中林宣男 渡边昭彦 《Medical Bulletin of Shanghai Jiaotong University》 CAS 1991年第2期75-80,共6页
Forty-seven kinds of materials were evaluated for their biocompatibility by a cell culture method of direct contact, including polyurethanes, silicone rubbers, polyvinyl chlorides, ethylene-vinyl acetate copolymers, p... Forty-seven kinds of materials were evaluated for their biocompatibility by a cell culture method of direct contact, including polyurethanes, silicone rubbers, polyvinyl chlorides, ethylene-vinyl acetate copolymers, polymethyl methacrylates, polyester, polypropylene, styrenebutadiene copolymer, thermoplastic elastomer, porcelains, and nickel chromium alloy, most of which were made in China as potential biomaterials. Most of the materials tested showed low cytotoxicity except polyvinyl chlorides and polyurethanes. 展开更多
关键词 cell culture BIOCOMPATIBILITY BIOMATERIALS
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The Effect of Three Culture Methods on Intensive Culture System of Pacific White Shrimp (Litopenaeus vannamei)
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作者 MA Zhen WAN Rong +1 位作者 SONG Xiefa GAO Lei 《Journal of Ocean University of China》 SCIE CAS 2013年第3期434-440,共7页
Different culture methods may affect the intensive culture system of Pacific white shrimp(Litopenaeus vannamei) regarding water quality and growth and economic performance.This study evaluated the potential effects of... Different culture methods may affect the intensive culture system of Pacific white shrimp(Litopenaeus vannamei) regarding water quality and growth and economic performance.This study evaluated the potential effects of three culture methods through cultivation of juvenile shrimps under consistent tank management conditions for 84 d.The three methods involved shrimp cultivation in different tanks,i.e.,outdoor tanks with cement bottom(mode-C),greenhouse tanks with cement bottom(mode-G) and outdoor tanks with mud-substrate(mode-M).Results showed that water temperature was significantly higher in mode-G than that in mode-C(P < 0.05).In contrast to the other two treatments,mode-M had stable pH after 50 d cultivation of shrimps.In the mid-late period,the average concentrations of TAN,NO2-N,DIP and COD were significantly lower in mode-M and mode-G compared with those in mode-C(P < 0.05).Despite lack of differences in the final shrimp weight among different treatments(P > 0.05),mode-M had significantly higher shrimp yield,survival rate and feed conversion rate(P < 0.05) than other modes.There were significant differences in revenue and net return among different treatments(P < 0.05).These demonstrated that the treatments of mode-G and mode-M were conductive to the intensive culture system of L.vannamei. 展开更多
关键词 culture method water quality growth performance economic performance Litopenaeus vannamei
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Study and promotion of safety culture using mixed methods research
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作者 Daiane Brigo Alves Elisiane Lorenzini +2 位作者 Nelly Oelke Anthony John Onwuegbuzie Adriane Cristina Bernat Kolankiewicz 《Frontiers of Nursing》 2021年第2期129-139,共11页
Objective:With a positive safety culture,institutions offer the best quality and safe care to their patients.The objective of this study was to analyze patient safety culture from the perspective of the multidisciplin... Objective:With a positive safety culture,institutions offer the best quality and safe care to their patients.The objective of this study was to analyze patient safety culture from the perspective of the multidisciplinary team,to identify factors that influence patient safety culture,and to create/promote-jointly with the study participants-strategies for improving processes of change.Methods:The study design represented a mixed methods research approach,with a sequential explanatory design.A multidisciplinary team of workers at a general hospital was eligible for the study.To collect quantitative data,we administered the Safety Attitudes Questionnaire(SAQ).The qualitative phase was accomplished via focus groups(FGs),with participants from the first phase of the study using the principles of deliberative dialogue(DD)as a knowledge-translation strategy.The STROBE guideline was used to develop the study.Results:The overall SAQ score was positive(75.1±10.4).Negative scores were found in the fields of Safety Climate,Working Conditions,and Stress Recognition.Focus group discussions identified the aspects that create a negative impact on safety culture,such as ineffective communication,punitive approach in the event of errors,the lack of commitment and adherence to the protocols,and the non-recognition of the stress and the mistakes.Actions for the promotion of safety culture were developed and implemented during the study.Conclusions:The use of the principles of DD as a strategy for knowledge translation(KT)made it possible to identify and plan for joint actions to generate improvements in safety culture. 展开更多
关键词 deliberative dialogue knowledge translation mixed methods organizational culture patient safety
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On C-E Translation Strategies and Methods of Zhiba Culture for Global Communication
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作者 YANG Jing 《Journal of Literature and Art Studies》 2021年第12期971-980,共10页
Zhiba culture is a profound culture with a long history,but there are still many problems in the C-E translation of it,such as low popularity,few recipients,and so on,which hinder its communication and development.The... Zhiba culture is a profound culture with a long history,but there are still many problems in the C-E translation of it,such as low popularity,few recipients,and so on,which hinder its communication and development.Therefore,it’s necessary to improve the quality of C-E translation of Zhiba culture.Based on the perspective of communication studies,combining with the principle of“Information First”,the principle of“The Audience First”,the paper explores the existing problems of Zhiba culture C-E translation and hopes to help to improve the present situation,to truly show the charm of Zhiba culture,to make it better accepted by the international community and optimize the global communication effect. 展开更多
关键词 Zhiba culture C-E translation COMMUNICATION translation strategies translation methods
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