Surface reconstruction of complex contour lines based on chain code matching technique

A new method for solving the tiling problem of surface reconstruction is proposed. The proposed method uses a snake algorithm to segment the original images, the contours are then transformed into strings by Freeman＇ s code. Symbolic string matching technique is applied to establish a correspondence between the two consecutive contours. The surface is composed of the pieces reconstructed from the correspondence points. Experimental results show that the proposed method exhibits a good behavior for the quality of surface reconstruction and its time complexity is proportional to mn where m and n are the numbers of vertices of the two consecutive slices, respectively.

Multiple fault diagnosis of down-hole conditions of sucker-rod pumping wells based on Freeman chain code and DCA

It is important to achieve continuous, stable and efficient pumping well operation in actual oilfield operation. Down-hole pumping well working conditions can be monitored in real-time and a reasonable production scheme can be designed when computer diagnosis is used. However, it is difficult to make a comprehensive analysis to supply efficient technical guidance for operation of the pumping well with multiple faults of down-hole conditions, which cannot be effectively dealt with by the common methods. To solve this problem, a method based on designated component analysis （DCA） is used in this paper. Freeman chain code is used to represent the down-hole dynamometer card whose important characteristics are extracted to construct a designated mode set. A control chart is used as a basis for fault detection. The upper and lower control lines on the control chart are determined from standard samples in normal working conditions. In an incompletely orthogonal mode, the designated mode set could be divided into some subsets in which the modes are completely orthogonal. The observed data is projected into each designated mode to realize fault detection according to the upper and lower control lines. The examples show that the proposed method can effectively diagnose multiple faults of down-hole conditions.

Face Recognition Using Chain Codes

Face recognition is an active area of biometrics. This study investigates the use of Chain Codes as features for recognition purpose. Firstly a segmentation method, based on skin color model was applied, followed by contour detection, then the chain codes of the contours were determined. The first difference of chain codes were calculated since the latter is invariant to rotation. The features were calculated and stored in a matrix. Experiments were performed using the University of Essex Face database, and results show a recognition rate of 95% with this method, when compared with Principal Components Analysis (PCA) giving 87.5% recognition rate.

REVERSE DESIGN APPROACH FOR MECHANISM TRAJECTORY BASED ON CODE-CHAINS MATCHING

Aiming at the problem of reverse-design of mechanism, a method based on the matching of trajectory code-chains is presented. The motion trajectory of mechanism is described with code-chain, which is normalized to simplify the operation of geometric transformation. The geometric transforma-tion formulas of scale, mirror and rotation for trajectory code-chain are defined, and the reverse de-sign for mechanism trajectory is realized through the analysis and solution of similarity matching between the desired trajectory and the predefined trajectory. The algorithm program and prototype system of reverse design for mechanism trajectory are developed. Application samples show that the method can break the restriction of trajectory patterns in matching, meet the demand of partial match-ing, and overcome the influence of geometric transformation of trajectory on the reverse design for mechanism.

Self-Dual Permutation Codes over Finite Chain Rings

Permutation codes over finite chain rings are introduced; by using the character of the finite chain rings and the knowledge of representation of group, some conditions for existence or non-existence of self-dual permutation codes over finite chain rings are obtained. Specially, when the group is a direct product of a 2-group and a T-group, and the group action is transitive, the sufficient and necessary condition of the existence of permutation codes is given.

非编码RNA在肺纤维化过程中的调控作用

背景:截至目前,肺纤维化的发病机制不明,治疗手段或者药物有限。大量研究发现,非编码RNA在肺纤维化的发生发展过程中发挥重要作用。目的:综述近年来非编码RNA在肺纤维化发病机制中的调控作用,深入了解肺纤维化的发病机制。方法:由第一作者应用计算机检索2000-2024年出版的文献,以“非编码RNA,微小RNA,长链非编码RNA,环状RNA,肺纤维化,综述”等为中文检索词检索中国知网、万方和维普数据库;以“ncRNA,miRNA,lncRNA,circRNA,Pulmonary fibrosis,review”等为英文检索词检索PubMed数据库,按照入选标准最终共纳入65篇文献进行综述。结果与结论:肺纤维化作为一种慢性且通常致命的肺部疾病,不仅可能自发产生,也可能是其他疾病的继发结果,主要特点是肺部间质中细胞外基质的异常增生和大量积累。非编码RNA是指由基因组转录出来的RNA分子,这些RNA分子并不涉及蛋白质的编码过程,凭借其调节能力已成为各种生物学现象中的一线分子参与者之一。非编码RNA在肺纤维化的发病机制中扮演着关键角色,微小RNA(miRNA)、长链非编码RNA(lncRNA)和环状RNA(circRNA)可以通过影响基因表达、转录后修饰以及细胞间的信号传导,参与到肺纤维化的发展过程中,为后续探索肺纤维化疾病的具体分子发生机制提供了新方向,为研发肺纤维化疾病的新靶向药物提供了理论依据及新思路。

Adding Sub-chain Method for Structural Synthesis of Planar Closed Kinematic Chains

For at least the past five decades,structural synthesis has been used as a main means of finding better mechanisms with some predefined function.In structural synthesis,isomorphism identification is still a problem unsolved well,and to solve this problem is very significant to the design of new mechanisms.According to the given degree of freedom（DOF） and link connection property of planar closed chain mechanisms,vertex assortment is obtained.For solving the isomorphism problem,a method of the adding sub-chains is proposed with the detailed steps and algorithms in the synthesizing process.Employing this method,the identification code and formation code of every topological structure are achieved,therefore many isomorphic structures could be eliminated in time during structural synthesis by comparing those codes among different topological graphs,resulting in the improvement of synthesizing efficiency and accuracy,and the approach for eliminating rigid sub-chains in and after the synthesizing process is also presented.Some examples are given,including how to add sub-chains,how to detect simple rigid sub-chains and how to obtain identification codes and formulation codes et al.Using the adding sub-chain method,the relative information of some common topological graphs is given in the form of table.The comparison result is coincident with many literatures,so the correctness of the adding sub-chain method is convinced.This method will greatly improve the synthesizing efficiency and accuracy,and has a good potential for application.

CYCLIC CODES OVER FORMAL POWER SERIES RINGS

In this article, cyclic codes and negacyclic codes over formal power series rings are studied. The structure of cyclic codes over this class of rings is given, and the relationship between these codes and cyclic codes over finite chain rings is obtained. Using an isomorphism between cyclic and negacyclic codes over formal power series rings, the structure of negacyclic codes over the formal power series rings is obtained.

CYCLIC AND NEGACYCLIC CODES OF LENGTH 2p^s OVER F_(p^m) + uF_(p^m)

In this article, we focus on cyclic and negacyclic codes of length 2p^s over the ring R = Fp^m ＋ uFp^m, where p is an odd prime. On the basis of the works of Dinh （in J.Algebra 324,940-950,2010）, we use the Chinese Remainder Theorem to establish the algebraic structure of cyclic and negacyclic codes of length 2p^s over the ring Fp^m ＋ uFp^m in terms of polynomial generators. Furthermore, we obtain the number of codewords in each of those cyclic and negacyclic codes.

缺血性脑卒中患者血清差异基因的筛选及生物信息学研究及验证

目的研究缺血性脑卒中患者血清差异基因的筛选及生物信息学。方法以2023年3月-2024年3月在新疆医科大学第二附属医院神经内科确诊的80例缺血性脑卒中患者为病例组,选择同期80例健康体检者为对照组。分别挑选两组各10例受试者的外周血清采用芯片差异性基因鉴定法筛选缺血性脑卒中差异表达的长链非编码RNA(lncRNA),并采用KEGG通路富集和基因本体论(GO)分析鉴定差异表达基因发挥的生物学功能。挑选2个上调和2个下调的lncR-NAs,在两组患者外周血中采用实时荧光定量PCR(qRT-PCR)法检测表达量,采用受试者工作特征曲线(Receiver operating characteristic,ROC)计算差异性表达lncRNAs诊断缺血性脑卒中的曲线下面积(Area under the curve,AUC)。结果共检测到34个高表达和16个低表达的lncR-NAs。KEGG通道分析显示,差异表达的lncRNAs涉及肿瘤坏死因子(TNF)信号通路、类风湿性关节炎、细胞因子与细胞因子受体相互作用,病毒蛋白与细胞因子和细胞因子受体的相互作用、癌症的转录失调、沙门氏菌感染、白细胞介素(IL)-17信号通路、趋化因子信号通路。GO分析显示,差异表达的lncRNAs涉及白细胞黏附调控、细胞黏附调节、白细胞与其他细胞黏附、细胞趋化性、T细胞活化、骨髓细胞分化、止血和凝血。qRT-PCR检测显示,与对照组比较,病例组患者A1BG-AS1和BRWD1-AS2表达量升高,BVES-AS1和C10ORF71-AS1表达量降低,差异有统计学意义(P<0.05)。ROC分析显示,A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1表达量诊断缺血性脑卒中的AUC分别为0.803、0.856、0.897和0.798(P<0.001)。结论缺血性脑卒中患者外周血中A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1基因差异性表达,可以辅助缺血性脑卒中的疾病诊断。

Network Coding for Wireless Sensor Network Cluster over Rayleigh Fading Channel: Finite State Markov Chain

Network Coding (NC) is confirmed to be power and bandwidth efficient technique, because of the less number of transmitted packets over the network. Wireless Sensor Network (WSN) is usually power limited network application, and in many scenarios it is power and bandwidth limited application. The proposed scenario in this paper applies the advantages of NC over WSN to obtain such power and bandwidth efficient WSN. To take the advantages of NC over the one of the most needed applications i.e., WSN, we come up to what this paper is discussing. We consider a WSN (or its cluster) that consists of M nodes that transmit equal-length information packets to a common destination node D over wireless Rayleigh block-fading channel where the instantaneous SNR is assumed to be constant over a single packet transmission period. Finite-State packet level Markov chain (FSMC) model is applied to give the channel more practical aspect. The simulation results showed that applying NC over the WSN cluster improved the channel bandwidth significantly by decreasing the number of the Automatic Repeat Request (ARQ), resulting in improving the power consumption significantly. The results are collected for different transmission distances to evaluate the behavior to the proposed scenario with regard to the bath losses effect.

血清FGL1、LncSChLAP1对晚期非小细胞肺癌免疫治疗效果及预后评估的价值

目的分析血清纤维蛋白样因子1(FGL1)、长链非编码RNA SChLAP1(LncSChLAP1)评估接受免疫治疗的晚期非小细胞肺癌(NSCLC)患者疗效及预后的价值。方法选取2019年4月—2022年12月南京市胸科医院呼吸内科诊治晚期NSCLC患者98例为NSCLC组,均接受免疫检查点抑制剂治疗,根据疗效分为有效亚组74例和无效亚组24例,以同期医院健康体检者50例为健康对照组。酶联免疫吸附实验检测NSCLC患者血清FGL1水平,实时荧光定量PCR检测血清LncSChLAP1水平;多因素Logistic回归分析影响晚期NSCLC患者化疗疗效的因素;受试者工作特征曲线分析血清FGL1、LncSChLAP1对晚期NSCLC患者免疫治疗疗效的预测价值;Kaplan-Meier曲线分析血清FGL1、LncSChLAP1对晚期NSCLC患者生存预后的影响。结果血清FGL1、LncSChLAP1水平比较,NSCLC组高于健康对照组(t/P=57.365/<0.001、28.443/<0.001)。无效亚组TNM分期Ⅳ期比例、血清FGL1、LncSChLAP1水平高于有效亚组(χ^(2)/P=15.375/<0.001,t/P=35.077/<0.001、35.127/<0.001)。血清FGL1、LncSChLAP1高是影响晚期NSCLC患者免疫治疗疗效的独立危险因素[OR(95%CI)=1.327(1.104~1.596)、1.415(1.094~1.829)];血清FGL1、LncSChLAP1及两项联合的AUC分别为0.856、0.792、0.905,两者联合优于各自单独预测效能(Z/P=4.258/0.007、5.119/<0.001)。FGL1高、低表达组1年总体生存率分别为22.92%(11/48)、60.00%(30/50),LncSChLAP1高、低表达组1年总体生存率分别为27.66%(13/47)、54.90%(28/51),FGL1高表达组、LncSChLAP1高表达组晚期NSCLC患者1年累积生存率分别低于FGL1低表达组、LncSChLAP1低表达组(χ^(2)/P=14.180/<0.001、17.553/<0.001)。结论晚期NSCLC患者血清FGL1、LncSChLAP1升高,是新的评估晚期NSCLC患者免疫治疗疗效及生存预后的血清标志物。

长链非编码RNA GC1靶向微小RNA-551b-3p抑制食管癌的作用及分子机制研究

目的探讨长链非编码RNA(LncRNA)鸟苷酸环化酶1(GC1)对食管癌的影响,并探讨其靶向微小RNA-551b-3p(miR-551b-3p)的分子机制。方法建立食管癌荷瘤裸小鼠模型,随机分为GC1、miR-551b-3p上、下调组及其对应对照组与模型组共9组,每组8只。末次给药次日处死裸小鼠,称取瘤体质量,计算抑瘤率;取肿瘤组织检测LncRNA GC1、miR-551b-3p基因表达水平和细胞周期因子(CyclinD1)、p21、B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关x(Bax)基因与蛋白表达水平;观察肿瘤组织病理改变;采用流式细胞术检测肿瘤细胞凋亡率;采用双荧光素酶报告基因实验验证LncRNA GC1与miR-551b-3p的调控关系。另取人食管癌细胞株Eca109分为9组,每组3个复孔,其中基因干扰组命名同上,另设置空白组,培养48h后观察细胞LncRNA GC1、miR-551b-3p基因表达水平、形态学改变、凋亡率、CyclinD1、p21、Bcl-2、Bax基因与蛋白表达水平。结果动物实验:与模型组和相应对照组比较,GC1上调组与miR-551b-3p下调组瘤体质量、CyclinD1和Bcl-2基因与蛋白表达水平均上升,抑瘤率、细胞凋亡率、miR-551b-3p基因、p21和Bax基因与蛋白表达水平均下降;GC1下调组与miR-551b-3p上调组瘤体质量、CyclinD1和Bcl-2基因与蛋白表达水平均下降,抑瘤率和细胞凋亡率、miR-551b-3p基因、p21和Bax基因与蛋白表达水平均上升;GC1上调组LncRNA GC1基因表达水平上升,GC1下调组LncRNA GC1基因表达水平下降(P<0.05)。与转染野生型GC1的腺病毒载体相比,转染突变型GC1的腺病毒载体的miR-551b-3p荧光素酶相对活性上升(P<0.05)。细胞实验:LncRNA GC1和miR-551b-3p基因表达水平、细胞形态学改变与凋亡率、CyclinD1、p21、Bcl-2、Bax基因与蛋白表达水平变化趋势均与动物实验相同。结论下调LncRNA GC1、上调miR-551b-3p表达水平均可抑制食管癌进展,且下调LncRNA GC1可靶向上调miR-551b-3p,并降低CyclinD1和Bcl-2活性、上升p21和Bax活性。

lncRNA H19和IGF2基因在乳腺癌组织中的表达水平及印记状态

目的:研究长链非编码RNA(lncRNA)H19和胰岛素样生长因子2(IGF2)基因在乳腺癌组织中的表达水平,分析其印记状态。方法:采用实时荧光定量PCR(RT-qPCR)法检测乳腺癌组织及癌旁组织中H19和IGF2 mRNA表达水平,分析H19和IGF2 mRNA在乳腺癌组织及癌旁组织中的表达差异,利用单核苷酸多态性(SNP)区分等位基因表达情况(纯合或杂合),基因组DNA中IGF2(ApaⅠ位点)或H19(AluⅠ位点)为杂合则进行印记分析,确定H19和IGF2在乳腺癌组织中的印记状态,即印记保持(MOI)或印记丢失(LOI)。分析乳腺癌组织中H19和IGF2表达与分子分型的关系。结果:RT-qPCR法检测,乳腺癌组织中H19和IGF2 mRNA表达水平高于癌旁组织(P<0.01),H19 mRNA表达水平与IGF2 mRNA表达水平呈正相关关系(r=0.567,P<0.01)。不同分子分型乳腺癌患者癌组织中H19 mRNA表达水平均高于癌旁组织(P<0.05或P<0.01)。H19和IGF2在乳腺癌组织中均存在LOI,IGF2的LOI发生率为36.7%,高于H19的LOI发生率(4.3%)。RT-qPCR法检测,IGF2 LOI组乳腺癌组织中IGF2 mRNA表达水平明显高于IGF2 MOI组(P<0.01)。结论:乳腺癌组织中H19和IGF2 mRNA表达水平明显高于癌旁组织,IGF2的LOI发生率高于H19的LOI发生率,IGF2的LOI可能是乳腺癌发病的关键因素之一。

NEAT1、miR-27a-3p在阿尔茨海默病患者血清和脑脊液中的表达关系

目的:探究长链非编码RNA核富含丰富的转录本1(long chain non-coding RNA nuclear-enriched abundant transcript 1, LncRNA NEAT1)、miR-27a-3p在阿尔茨海默病(Alzheimer disease, AD)患者血清和脑脊液中的表达关系及意义。方法:选择2019年10月至2021年9月天津市第五中心医院神经内科收治的AD患者66例作为病例组,根据临床痴呆评定量表(clinical dementia rating, CDR)评分分为轻度组(≤1分,n=41)与中重度组(>1分,n=25);另取同期门诊其他就诊患者血清和脑脊液标本66例志愿者作为对照组。收集所有受试者的一般资料并评估认知程度,采用实时荧光定量PCR检测血清和脑脊液miR-27a-3p、NEAT1表达水平,酶联免疫吸附试验检测脑脊液β-淀粉样前体蛋白裂解酶1(β-site amyloid precursor protein cleaving enzyme 1,BACE1)、β淀粉样蛋白(amyloid β,Aβ)40和Aβ42水平,采用Spearman法分析血清miR-27a-3p、NEAT1水平与简易精神状态检测量表的相关性,采用Pearson法分析血清miR-27a-3p、NEAT1水平与Aβ沉积平均标准摄取值比率(standardized uptake value ratio, SUVR)及脑脊液miR-27a-3p、NEAT1、BACE1、Aβ42、Aβ40水平的相关性。结果:MMSE评分[21 (17,25),9(7,11)vs. 27 (21,34)]、MoCA评分[17 (12,21),10 (7,13)vs. 27 (21,31)]、血清miR-27a-3p水平(0.55±0.13,0.46±0.06 vs. 0.97±0.22)、脑脊液miR-27a-3p(0.48±0.10,0.35±0.10 vs. 1.03±0.31)、Aβ42水平[(303.55±36.77) ng/L,(231.45±34.14) ng/L vs.(499.99±53.63) ng/L]及Aβ42/Aβ40比值(0.030±0.008, 0.022±0.007 vs. 0.048±0.010)轻度组、中重度组AD患者均低于对照组(P均<0.05),且中重度组AD患者较轻度组低(P均<0.05);血清NEAT1水平(2.31±0.64,3.13±0.76 vs. 1.05±0.20)、SUVR(1.50±0.29,1.76±0.52 vs. 0.74±0.15)及脑脊液NEAT1(3.51±1.24,4.30±1.65 vs. 1.01±0.23)、BACE1水平[(55.78±5.98)μg/L,(72.32±16.08)μg/L vs.(21.39±3.73)μg/L]轻度组、中重度组AD患者均高于对照组(P均<0.05),且中重度组AD患者较轻度组高(P均<0.05)。AD患者血清NEAT1水平与SUVR及脑脊液NEAT1、BACE1呈正相关(r=0.350,0.606,0.341,P<0.05),与MMSE评分、MoCA评分呈负相关(r=-0.473,-0.482,P均<0.05);血清miR-27a-3p水平与脑脊液miR-27a-3p水平、MMSE评分、MoCA评分呈正相关(r=0.695,0.424,0.412,P<0.05),与SUVR及脑脊液BACE1水平呈负相关(r=-0.521、-0.447,P均<0.05)。结论:NEAT1、miR-27a-3p在AD患者血清及脑脊液中表达趋势具有一致性,NEAT1水平均升高,miR-27a-3p水平均降低,二者水平呈负相关,与AD患者脑中Aβ沉积程度有关,并参与AD的病情进展。

急性缺血性脑卒中患者血清长链非编码RNA母系表达基因3和Zeste同源物增强子-2表达与神经功能损伤的相关性分析

目的探讨长链非编码RNA(lncRNA)母系表达基因3(MEG3)与Zeste同源物增强子-2(EZH2)在急性缺血性脑卒中(AIS)患者血清中的表达水平及其与神经功能损伤之间的关系。方法选取延安市人民医院2021年1月至2022年11月收治的92例AIS患者为脑卒中组,92例健康体检者为对照组,根据NIHSS评分将脑卒中组患者分为致残性损伤组19例,非致残性损伤组73例。采用实时荧光定量聚合酶链式反应、酶联免疫吸附法分别检测血清lncRNAMEG3、EZH2水平。采用Spearman相关分析法进行AIS患者血清lncRNAMEG3、EZH2表达水平与美国国立卫生研究院卒中量表(NIHSS)评分之间的相关性分析;采用多因素Logistic回归分析AIS患者合并神经功能致残性损伤的影响因素,并绘制受试者工作特征(ROC)曲线分析血清lncRNA MEG3、EZH2水平对AIS患者合并神经功能致残性损伤的诊断价值。结果脑卒中组患者血清lncRNA MEG3、EZH2表达水平均高于对照组(t=11.817、11.542,P<0.05)。Spearman相关分析显示,AIS患者血清lncRNA MEG3、EZH2水平与NIHSS评分均呈正相关(r=0.540、0.603,P<0.01)。致残性损伤组体质量指数、吸烟史占比、甘油三酯、总胆固醇、同型半胱氨酸、发病-到院时间(ODT)、lncRNAMEG3、EZH2水平均高于非致残性损伤组(t/χ2=2.103、5.050、9.121、5.585、2.276、5.448、4.638、4.682,P<0.05)。多因素Logistic回归分析显示,甘油三酯、总胆固醇、lncRNA MEG3、EZH2以及ODT均是AIS患者合并神经功能致残性损伤的影响因素(OR=4.853、4.277、2.674、3.052、3.901,P<0.05)。lncRNA MEG3、EZH2联合诊断AIS患者合并神经功能致残性损伤的曲线下面积(AUC)大于lncRNAMEG3以及EZH2单独诊断的AUC(Z=2.626、2.954,P<0.01),敏感度、特异度分别为94.74%、82.15%。结论AIS患者血清lncRNA MEG3、EZH2水平均上升,与AIS患者神经功能损伤程度均正相关,可用作AIS患者合并神经功能致残性损伤的诊断指标,且联合诊断效果更好。

Construction of Self-dual Codes over F_p+vF_p

In this paper, we give an explicit construction for self-dual codes over F_p+vF_p(v^2= v) and determine all the self-dual codes over F_p+ vF_p by using self-dual codes over finite field F_p, where p is a prime.

ON THE CHARACTERIZATION OF CYCLIC CODES OVER TWO CLASSES OF RINGS

Let R be a finite chain ring with maximal ideal （7） and residue field F,and letγ be of nilpotency index t. To every code C of length n over R, a tower of codes C = （C ： γ0） C_ （C： 7） C ... C_ （C： γ2） C_ .-. C_ （C：γ^t-1） can be associated with C, where for any r C R, （C ： r） = {e C Rn I re E C}. Using generator elements of the projection of such a tower of codes to the residue field F, we characterize cyclic codes over R. This characterization turns the condition for codes over R to be cyclic into one for codes over the residue field F. Furthermore, we obtain a characterization of cyclic codes over the formal power series ring of a finite chain ring.

长链非编码RNA TUG1和UCA1在结肠癌组织中的表达及临床意义

目的探究长链非编码RNA TUG1和UCA1在结肠癌组织中的表达及临床意义。方法选取2018年5月—2020年5月收治的80例结肠癌患者为研究对象,利用原位杂交与qRT-PCR法检测TUG1和UCA1的表达,对表达情况和患者病理特征及预后进行研究。结果UCA1和TUG1在结直肠癌组织表达过程中与性别、年龄以及病理情况关系较小(P>0.05),和患者肿瘤大小、是否存在淋巴结转移、疾病的分化情况以及分期情况高度相关(P<0.05)。UCA1和TUG1高表达患者肿瘤更大,淋巴结存在转移情况,分化程度较高且TNM分期以Ⅰ/Ⅱ为主;80例患者进行随访36个月,中位生存时间33.75个月,死亡人数16例,术后总生存率80.00%(64/80);Kaplan-Meier生存分析显示UCA1和TUG1高表达死亡12例,生存38例,生存率76.00%(38/50);UCA1和TUG1低表达死亡4例,生存26例,生存率86.66%(26/30)。结论直肠癌组织中lncRNA TUG1和UCA1高表达与低表达对患者疾病预后有一定影响。

间充质干细胞对糖尿病肾病小鼠lncRNA的干预及肾脏保护的可能机制

目的 筛选糖尿病肾病(DN)小鼠模型中异常表达的长链非编码RNA(lncRNA),探索与间充质干细胞(MSC)干预可能的lncRNA是否对小鼠肾脏起保护作用及可能机制。方法 利用基因表达综合数据库(GEO)来源的转录组测序(RNAseq)数据,对DN小鼠RNAseq数据与正常小鼠进行筛选,得到与DN相关的lncRNA并进行验证,得到上调前3名的lncRNA,选取变化最显著的lncRNA进行下一步实验。分离培养小鼠肾小管上皮细胞(mRTECs)及骨髓间充质干细胞(BMSC),分组培养mRTECs[对照组、高糖(HG)组、HG+BMSC组],四甲基偶氮唑蓝(MTT)检测各组mRTECs细胞增殖率,荧光定量PCR法检测各组mRTECs中胶原蛋白(collagen)Ⅰ、α-平滑肌肌动蛋白(SMA)、波形蛋白(vimentin)、E-钙黏蛋白(cadherin) mRNA表达。采用siRNA敲减mRTECs中lncRNA的表达,构建正确的pcDNA3.1(+)质粒扩大培养。分组培养mRTECs(对照组、NC组、lncRNA组;si-NC组、si-lncRNA组、HG组、HG+si-lncRNA组;HG+BMSC组、HG+BMSC+lncRNA组),Western印迹检测各组细胞中collagenⅠ、α-SMA、vimentin、E-cadherin蛋白表达。结果 上调前3位的lncRNA为lncRNA NONMMUG023935、lncRNA NONMMUG030287、lncRNA NONMMUG004302。mRTECS经HG处理后,细胞增殖率、collagenⅠ、α-SMA和vimentin及lncRNA NONMMUG023935、lncRNA NONMMUG030287、lncRNA NONMMUG004302表达显著上升,E-cadherin表达显著下降(P<0.05)。而加入BMSC的mRTECs经HG处理后,上述指标的结果相反(P<0.05)。根据上述3个lncRNA表达变化绝对值最大选取lncRNA NONMMUG023935进行后续实验。转染si-lncRNA-NONMMUG023935后,相对应的lncRNA-NONMMUG023935表达显著降低(P<0.01)。lncRNA NONMMUG023935过表达可促进对mRTECs的损伤(P<0.05),BMSC可通过下调lncRNA NONMMUG023935表达从而抑制对HG诱导的mRTECs的损伤(P<0.05)。结论 MSC可下调DN肾小管上皮细胞中lncRNA NONMMUG023935的表达,从而改善肾脏纤维化,这可能是MSC延缓DN进展的机制之一。