As one of the important means to spread Chinese traditional culture,Chinese-style animation has been on the rise in recent years.Many excellent works have emerged,among which the Chinese animated short film series Yao...As one of the important means to spread Chinese traditional culture,Chinese-style animation has been on the rise in recent years.Many excellent works have emerged,among which the Chinese animated short film series Yao-Chinese Folktales is one of the good representatives.It aroused a strong reaction from the audience as soon as it was released on the Internet,and gave the audience a shock to their mind and vision,which has brought some thoughts to the development of Chinese-style animation.Following a linear logic chain of past-present-future Chinese-style animation’s development,this paper takes Yao-Chinese Folktales as an example and analyzes the developmental difficulties and prospects of Chinese-style animation.At the end of the paper,some suggestions about the development path of Chinese-style animation’s future are given from the aspects of creation and dissemination,for the aim of promoting the Chinese-style animation’s development and spreading the Chinese traditional culture.展开更多
In recent years,traditional Chinese culture has been continuously revived,integrated into the times and entered people’s daily lives.Chinese-style songs,as a combination of traditional cultural connotations and emerg...In recent years,traditional Chinese culture has been continuously revived,integrated into the times and entered people’s daily lives.Chinese-style songs,as a combination of traditional cultural connotations and emerging song forms,have gradually emerged on the music stage in China and even around the world.In the field of teaching Chinese as a second language,the research on teaching Chinese songs has received increasing attention.This article combines the theory of the association between music and language to summarize the research results of Chinese-style songs and their application in the field of teaching Chinese as a second language in the past 30 years.The current research problems are discussed,and suggestions are proposed in order to provide some reference value for teaching Chinese as a second language.展开更多
The emergence and development of Chinese-style architecture in European gardens in the 18;century were studied, and the influence and significance of Chinese-style architecture in the development of European gardens w...The emergence and development of Chinese-style architecture in European gardens in the 18;century were studied, and the influence and significance of Chinese-style architecture in the development of European gardens were analyzed.展开更多
目的 研究制备 nor A基因介导的金黄色葡萄球菌对氟喹诺酮类药物的耐药机制的 Dig- nor A基因探针。方法 采用聚合酶链反应 (PCR)制备 Dig- nor A基因探针。结果 PCR法制备 Dig- nor A基因探针简便易行 ,可在较短时间内获得大量的探...目的 研究制备 nor A基因介导的金黄色葡萄球菌对氟喹诺酮类药物的耐药机制的 Dig- nor A基因探针。方法 采用聚合酶链反应 (PCR)制备 Dig- nor A基因探针。结果 PCR法制备 Dig- nor A基因探针简便易行 ,可在较短时间内获得大量的探针 ,所得探针有较高的敏感性 ;Dig- nor A基因探针安全、易操作 ,标记探针可长期保存。结论 为进一步研究 nor展开更多
按金黄色葡萄球菌多重耐药转运蛋白NorA的编码序列设计引物,以金黄色葡萄球菌基因组DNA为模板,扩增出norA基因中1 155 bp cDNA片段,将所得片段与pMD18-T载体连接,转化到感受态大肠杆菌JM109中,成功地筛选到阳性克隆,其质粒测序结果与文...按金黄色葡萄球菌多重耐药转运蛋白NorA的编码序列设计引物,以金黄色葡萄球菌基因组DNA为模板,扩增出norA基因中1 155 bp cDNA片段,将所得片段与pMD18-T载体连接,转化到感受态大肠杆菌JM109中,成功地筛选到阳性克隆,其质粒测序结果与文献报道一致。从阳性克隆中提取质粒,经NdeⅠ和XhoⅠ酶切,回收1 155 bp目的片段,定向克隆到pET-28a(+)表达载体中,转化感受态大肠杆菌DH5α,提取质粒,再次转化到BL21(DE3)中,成功地筛选到阳性克隆。经IPTG诱导阳性菌,通过SDS-PAGE检测出norA基因的表达。展开更多
金黄色葡萄球菌的 Nor A蛋白是一种跨膜多重药物外排蛋白 ,介导了金黄色葡萄球菌对氟喹诺酮类药物的耐药。Nor A蛋白属于细菌转运系统中的主要易化族类 ,由位于染色体上的 nor A基因编码。nor A基因大小约为 1.8kb,编码 388个氨基酸构...金黄色葡萄球菌的 Nor A蛋白是一种跨膜多重药物外排蛋白 ,介导了金黄色葡萄球菌对氟喹诺酮类药物的耐药。Nor A蛋白属于细菌转运系统中的主要易化族类 ,由位于染色体上的 nor A基因编码。nor A基因大小约为 1.8kb,编码 388个氨基酸构成的多肽。 Nor A蛋白富含疏水性氨基酸 ,可能构建了亲水性氟喹诺酮类药物的膜相关性活性外排泵 ,主要介导了亲水性氟喹诺酮类药物从细胞中主动排出 ,研究认为这主要是由于膜上 Nor A蛋白数量的增加造成的。目前 nor A超水平表达的调控机制尚不清楚 ,部分人认为是 nor A基因多处位点或 (和 )启动子区域发生突变 ,影响了 nor A的表达。已证明利血平、维拉帕米和 5′- methoxyhydnocarpin等是 Nor A外排蛋白的抑制剂 ,能增强亲水性氟喹诺酮类药物的抗菌活性 ,降低耐药率。展开更多
文摘As one of the important means to spread Chinese traditional culture,Chinese-style animation has been on the rise in recent years.Many excellent works have emerged,among which the Chinese animated short film series Yao-Chinese Folktales is one of the good representatives.It aroused a strong reaction from the audience as soon as it was released on the Internet,and gave the audience a shock to their mind and vision,which has brought some thoughts to the development of Chinese-style animation.Following a linear logic chain of past-present-future Chinese-style animation’s development,this paper takes Yao-Chinese Folktales as an example and analyzes the developmental difficulties and prospects of Chinese-style animation.At the end of the paper,some suggestions about the development path of Chinese-style animation’s future are given from the aspects of creation and dissemination,for the aim of promoting the Chinese-style animation’s development and spreading the Chinese traditional culture.
文摘In recent years,traditional Chinese culture has been continuously revived,integrated into the times and entered people’s daily lives.Chinese-style songs,as a combination of traditional cultural connotations and emerging song forms,have gradually emerged on the music stage in China and even around the world.In the field of teaching Chinese as a second language,the research on teaching Chinese songs has received increasing attention.This article combines the theory of the association between music and language to summarize the research results of Chinese-style songs and their application in the field of teaching Chinese as a second language in the past 30 years.The current research problems are discussed,and suggestions are proposed in order to provide some reference value for teaching Chinese as a second language.
文摘The emergence and development of Chinese-style architecture in European gardens in the 18;century were studied, and the influence and significance of Chinese-style architecture in the development of European gardens were analyzed.
文摘目的 研究制备 nor A基因介导的金黄色葡萄球菌对氟喹诺酮类药物的耐药机制的 Dig- nor A基因探针。方法 采用聚合酶链反应 (PCR)制备 Dig- nor A基因探针。结果 PCR法制备 Dig- nor A基因探针简便易行 ,可在较短时间内获得大量的探针 ,所得探针有较高的敏感性 ;Dig- nor A基因探针安全、易操作 ,标记探针可长期保存。结论 为进一步研究 nor
文摘金黄色葡萄球菌的 Nor A蛋白是一种跨膜多重药物外排蛋白 ,介导了金黄色葡萄球菌对氟喹诺酮类药物的耐药。Nor A蛋白属于细菌转运系统中的主要易化族类 ,由位于染色体上的 nor A基因编码。nor A基因大小约为 1.8kb,编码 388个氨基酸构成的多肽。 Nor A蛋白富含疏水性氨基酸 ,可能构建了亲水性氟喹诺酮类药物的膜相关性活性外排泵 ,主要介导了亲水性氟喹诺酮类药物从细胞中主动排出 ,研究认为这主要是由于膜上 Nor A蛋白数量的增加造成的。目前 nor A超水平表达的调控机制尚不清楚 ,部分人认为是 nor A基因多处位点或 (和 )启动子区域发生突变 ,影响了 nor A的表达。已证明利血平、维拉帕米和 5′- methoxyhydnocarpin等是 Nor A外排蛋白的抑制剂 ,能增强亲水性氟喹诺酮类药物的抗菌活性 ,降低耐药率。
