In order to develop organic rice and increase paddy soil fertility by cloverorganic rice rotation, the effects of N, P and K fertilizers on growth of clover nitrogen-fixing rhizobia and soil fertility after plantation...In order to develop organic rice and increase paddy soil fertility by cloverorganic rice rotation, the effects of N, P and K fertilizers on growth of clover nitrogen-fixing rhizobia and soil fertility after plantation were investigated, thereby providing certain reference for reasonable cultivation of clover and improvement of soil fertility. A two-year experiment was conducted from 2012 to 2013. The clover was cultivated after rice every year, and different levels of N, P and K fertilizers were applied before winter. In the treatment group, no fertilizer was applied. The effects of different fertilizers and different application amounts on clover yield, nitrogen-fixing rhizobia quantity, nitrogen-fixing rhizobia weight and soil fertility after plantation were analyzed. The results showed that the application of N, P and K fertilizers in the early stage had significantly effect on the growth of clover. When the application amount of N fertilizer was 75 kg/hm^2(N 46%), the clover yield, nitrogen-fixing rhizobia quantity and nitrogen-fixing rhizobia weight were highest. The soil nitrogen content after plantation increased with the increase of the application amount of N fertilizer, while the P and K content decreased and then increased with the increased application amounts. Soil available P content increased with the increased application amounts of N and P fertilizers, but it did not change significantly with the increased application amount of K fertilizer. Soil available K content increased first and then decreased with the increased application amounts of N and P fertilizers.When the application amounts of N and P fertilizers were 150(N 46%) and 300(P_2O_5 12%) kg/hm^2, soil available K content reached the maximum. Soil organic matter content increased with the increased application amounts of N, P and K fertilizers. Therefore, in the cultivation of clover, appropriate application of N, P and K fertilizers in the early stage can improve clover yield and soil fertility.展开更多
[Objective] The study aimed to explore the degradation law and trend of artificial grassland. [Method] Taking the ryegrass (Lolium perenne) - white clover ( Trifolium repens) artificial grassland in Maiping Townsh...[Objective] The study aimed to explore the degradation law and trend of artificial grassland. [Method] Taking the ryegrass (Lolium perenne) - white clover ( Trifolium repens) artificial grassland in Maiping Township, Guizhou Province as the research object, the grassland vegetation of 40 quadrate from different areas (area around the sheep shed, hilltop, hillside, flatland at the foot of the hill) were analyzed by comparing the dominance and richness index. [ Result] Degradation of different degrees appeared in various areas of this artificial grassland. To be specific, the degradation of grassland around the sheep shed was not serious for less consumption by the sheep; however, the areas on the hilltop and hillside degenerated to the grassland types of dallis grass ( Paspalum dilatatum Poir. ) -Indian lovegrass ( Eragrostis pilosa) and dallis grass - cogongrass [ Imperata cylindrica ( Linn. ) Beauv. ], respectively, and the area at the foot of the hill degenerated to the grassland type dominated by garland chrysanthetnum ( Chrysanthemum coronarium L. ) and knotgrass ( Paspalum distichum L. ). [ Conclusion ] This study provided a basis for grassland improvement as well as the efficient and sustainable utilization of grazing-type artificial grassland in South China.展开更多
The capacities of two arbuscular mycorrhizal (AM) fungi, Glomusmosseae and Glomus versiforme, to mineralize added organic P werestudied in a sterilized clacareous soil. Mycorrhizal (inoculated witheither of the AM fun...The capacities of two arbuscular mycorrhizal (AM) fungi, Glomusmosseae and Glomus versiforme, to mineralize added organic P werestudied in a sterilized clacareous soil. Mycorrhizal (inoculated witheither of the AM fungi) and non-mycorrhizal red clover (Trifoliumpratense L.) plants were grown for eight weeks in pots with upperroot, central hyphal and lower soil compartments. The hyphal and soilcompartments received either organic P (as Na-phytte) or inorganic P(as KH_2PO_4) at the rate of 50 mg P kg^-1. No P was added to theroot compartments.展开更多
Phytoremediation has been used as an emerging technology for remediation of soil contamination with polycyclic aromatic hydrocarbons(PAHs),ubiquitous persistent environmental pollutants derived from natural and anthro...Phytoremediation has been used as an emerging technology for remediation of soil contamination with polycyclic aromatic hydrocarbons(PAHs),ubiquitous persistent environmental pollutants derived from natural and anthropogenic processes,in the last decade.In this study,a pot experiment was conducted to investigate the potential of phytoremediation of pyrene from spiked soils planted with white clover(Trifolium repens)in the greenhouse with a series of pyrene concentrations ranging from 4.22 to 365.38 mg kg-1.The results showed that growth of white clover on pyrenecontaminated soils was not affected.The removal of pyrene from the spiked soils planted with white clover was obviously higher than that from the unplanted soils.At the end of the experiment(60 d),the average removal ratio of pyrene in the spiked soils with white clover was 77%,which was 31%and 57%higher than those of the controls with or without micobes, respectively.Both roots and shoots of white clover took up pyrene from the spiked soils and pyrene uptake increased with the soil pyrene concentration.However,the plant-enhanced dissipation of soil pyrene may be the result of plant-promoted microbial degradation and direct uptake and accumulation of pyrene by white clover were only a small part of the pyrene dissipation.Bioconcentration factors of pyrene(BCFs,ratio of pyrene,on a dry weight basis,in the plant to that in the soil)tended to decrease with increase in the residual soil pyrene concentration.Therefore,removal of pyrene in the contaminated soils was feasible using white clove.展开更多
Globally, white clover(Trifolium repens L.) is commonly infected by plant viruses. It is grown at gardens, roadsides, and public areas as ornamental plants in northern China. Some leaves present disease symptoms tha...Globally, white clover(Trifolium repens L.) is commonly infected by plant viruses. It is grown at gardens, roadsides, and public areas as ornamental plants in northern China. Some leaves present disease symptoms that are similar to those of virus infection. However, to our knowledge, no records are available from China regarding white clover(Trifolium repens L.) virus co-infection. To determine the viral species that infect white clover in China, plant samples with virus disease symptoms were collected and virion morphology and ultrastructure morphology of co-infected plants were observed by electron microscopy; viruses were detected by enzyme linked immunosorbent assay(ELISA) and reverse transcription PCR(RT-PCR). Virus co-infection was studied by double antibody sandwich enzyme linked immunosorbent assay(DAS-ELISA) and real-time fluorescence quantitative PCR(RT-q PCR) after rub-inoculation of virus-free white clover planted in a growth chamber(25°C) with a photoperiod of 16 h, using single or several purified virions. Results showed that there were six types of symptoms, including those of shrinking mosaic, shrinking and macular mosaic, severe mottle mosaic, yellow macular mosaic, shrinkage chlorisis, and ring plaque and ring stria. The incidence rates for each symptom were 20.93, 1.48, 16.85, 59.07, 1.30, and 0.37%, respectively, based on the field investigation. Two types of viral pathogens were identified as Alfalfa mosaic virus(AMV) and White clover mosaic virus(WCMV). In mesophyll cells, virus particles with bacilliform virions formed aggregates and linear virions were bundle shaped. The detection rate of AMV was 100% in white clover samples by DAS-ELISA and RT-PCR detection, whereas that of WCMV was 83.33%. The co-infection rate was 83.33%. The relative contents of AMV and WCMV were significantly increased by 5.897-and 3.515-fold upon co-infection, when compared to that with single virus infection. We observed larger starch particles and fewer or collapsed chloroplast grana in co-infected plants; in addition, vacuoles were twisted and smaller, compared to those of healthy plants. To our knowledge, this is the first report of co-infection by AMV and WCMV in white clover, which has caused severe mosaicism and ultrastructure lesions in co-infected plants in China.展开更多
In December of 3 years, 87 beef cows with nursing calves (.594 ± 9.8 kg; calving season, September to November) at side were stratified by body condition score, body weight, cow age, and calf gender and divided...In December of 3 years, 87 beef cows with nursing calves (.594 ± 9.8 kg; calving season, September to November) at side were stratified by body condition score, body weight, cow age, and calf gender and divided randomly into 6 groups assigned to 1 of 6 cool-season annual pastures (0.45 ha/cow) that had been interseeded into a dormant common bermudagrass (Cynodon dactylon [L.] Pers.)/bahiagrass (Paspalum notatum Flugge) sod. Pastures contained 1 of the following 3 seeding mixtures (2 pastures/mixture): 1) wheat (Triticum aestivurn L.) and ryegrass (Lofium multiflorum Lain., WRG), 2) wheat and ryegrass plus red clover (Trifolium pretense L, WRR), or 3) wheat and ryegrass plus white (Trifofium repens L.) and crimson clovers (Trifolium incarnatum L., WRW). All groups had ad libitum access to grass hay (12% crude protein; 58% total digestible nutrients). The second week in December, cow estrous cycles were synchronized and artificially inseminated. In late December, a bull was placed with each group for 60-d. Data were analyzed with an analysis of variance using a mixed model containing treatment as the fixed effect and year as the random effect. Body weight and condition scores did not differ (P ≥ 0.27) among cows between February and June. Calf birth weights or average daily gain did not differ (P≥ 0.17) among treatments; however, calves grazing pastures with clovers did tend (P= 0.06) to weigh more than calves grazing grass only. Weaning weight per cow exposed to a bull was greater (P= 0.02) for WRR and WRW than WRG. Cows grazing winter-annual pastures containing clovers tended to wean more calf body weight per cow exposed to a bull than cows grazing the grass only pastures.展开更多
The effect of die inlet and transition geometry on the extrusion loads and ~aaterial flow for extrusion of clover sections were investigated and presented both theoretically and experimentally. For this purpose, four ...The effect of die inlet and transition geometry on the extrusion loads and ~aaterial flow for extrusion of clover sections were investigated and presented both theoretically and experimentally. For this purpose, four different die geometries including straight tapered and cosine transition profile and each of them having round and clover inlet geometries were chosen. In the experimental study, commercially pure lead was used because of its hot forming characteristic at room temperature. A newly kinematical admissible velocity field to analyze different profiles of extrusion dies of clover section from round bars was proposed by upper bound analysis. It is clear that the extrusion loads obtained from the theoretical analysis for various die inlet-die transition geometry combinations are in good agreement with the experimental results. Axis deviations of the parts which define the dimensional quality of the products were also investigated.展开更多
Laser assisted turning (LAT) is one of the advanced machining technologies, which uses laser power to heat the surface of a workpiece before the material is removed. It has several advantages of low manufacturing co...Laser assisted turning (LAT) is one of the advanced machining technologies, which uses laser power to heat the surface of a workpiece before the material is removed. It has several advantages of low manufacturing costs, high productivity and high qualities to machine difficult-to-cut materials such as silicon nitride, muUite, zirconia and Ni. A large part of studies on LAT have been focused on a round bar. With increasing demands for high quality products and high performance engineering system, the researches on LAT for clover and square section members are necessary. But, these workpieces are impossible to be machined on conventional CNC lathe and to generate NC code with current CAM softwares. As a basic research for combining LAT with a tilting index table type 5-axis machining center, i.e. laser assisted turn-mill, a new method is suggested to generate NC code that can process various types of clover and square section members through development of C++ program.展开更多
Although white clover has been widely studied and applied,less is known about its physiological responses to chilling stress in northern China.The effects of acclimation time of chilling stress in the two white clover...Although white clover has been widely studied and applied,less is known about its physiological responses to chilling stress in northern China.The effects of acclimation time of chilling stress in the two white clover cultivars,Rivendel and Haifa,after exposure to 4℃for 0,4,8,12,24 and 48 h were investigated.Seven physiological indexes were measured in shoots of the two cultivars.It was found that Rivendel had a strong ability to maintain the stability of cells,cope with oxidative damage and accumulate osmotic adjustment substances compared with Haifa.In addition,a comprehensive evaluation on the chilling tolerance of the two cultivars based on subordinate function value analysis showed a stronger chilling tolerance of Rivendel than Haifa.展开更多
Effect of seeding rate mixture from Egyptian clover (Trifolium alexandrinum L.) E.C. and barley (Hordum vulgare L.) B, N-fertilization levels and their interaction on quantity and quality of forage yield. Two field ex...Effect of seeding rate mixture from Egyptian clover (Trifolium alexandrinum L.) E.C. and barley (Hordum vulgare L.) B, N-fertilization levels and their interaction on quantity and quality of forage yield. Two field experiments were conducted during winter season of 2012/2013 and 2013/2014 in sandy soil at research and production station of National Research Centre, Al Nubaria district, Al Behaira Governorate, Egypt. The experiment included two factors first was five mixtures from seeding rates (24 kg EC/fed. – 50 kg B/fed. – 18 kg EC + 12.5 kg B/fed. – 12kg EC + 25 kg B/fed. – 6 kg EC + 37.5 kg B/fed.) and second factor was three N levels (30-45-60 kg N/fed.). Two cuts were obtained at 60 and 100 DAS. Results indicated significant differences between mixture rates for all studied characters in both cuts 60 and 100 DAS. Significant differences between N levels for all studied characters at 60 DAS, also, forage yield /fed. and DM% at 100 DAS. It can be introducing forage mixture of 18 kg EC + 12.5 B had balanced character in forage yield/fed., DM%, carbohydrate % and protein % at both cuts 60 and 100 DAS.展开更多
Agroforestry systems have the potential to provide year-long income opportunities via the integrated forage or crop, timber, and livestock. Legumes are an attractive alternative option during the growing season when m...Agroforestry systems have the potential to provide year-long income opportunities via the integrated forage or crop, timber, and livestock. Legumes are an attractive alternative option during the growing season when more traditional forages may not be as productive. The objective of this study was to test the establishment of arrowleaf and white clover grown under varying pine tree alley widths. In 2011, existing forage was removed in 15-yr old loblolly pine tree row alleys of different widths (3.7, 4.9, 7.3, and 9.8 m), including an open area. Arrowleaf, as an annual, was replanted in 2012. Seedlings were counted twice/year, while dry matter was measured three times/year. Photosynthetically active radiation (PAR) was measured in all alley widths to compare light penetration through the canopy. Hot and dry conditions occurred throughout 2012, affecting results. In 2012 and 2013, the greatest PAR for most treatments was observed in June. Seedling counts for all treatments were greatest immediately after establishment, and gradually declined throughout the course of the study. Dry matter yields increased throughout the growing season, and were greatest in arrowleaf clover in the open area on all measurement dates;however, increased weed pressure and repeated flooding affected yields. This study demonstrated that clover establishment in shady wooded areas is possible, but only under suitable environmental conditions.展开更多
Underseeding red clover in winter wheat is a beneficial agronomic practice. Still, many growers tend to forgo this approach. One reason is that herbicides used on winter wheat may injure underseeded red clover, reduci...Underseeding red clover in winter wheat is a beneficial agronomic practice. Still, many growers tend to forgo this approach. One reason is that herbicides used on winter wheat may injure underseeded red clover, reducing its biomass and the subsequent benefits it provides. Therefore, the effect of winter wheat herbicides on underseeded red clover needs to be evaluated. The objectives of this research were to assess the crop tolerance of underseeded red clover to ten winter wheat herbicides used in Ontario, Canada and determine if red clover tolerance differed when the herbicides were applied at various winter wheat growth stages. Experiments were conducted in 2009 and 2010 at four different Ontario locations. Each herbicide treatment was either applied at an early, normal or late timing. Overall, red clover was not affected by herbicides applied at the early timing. The likelihood of herbicides causing injury and reducing biomass of underseeded red clover increased when they were applied at the more advanced winter wheat growth stages. If timing is a constraint, the three herbicides bromoxynil/MCPA, tralkoxydim, and fenoxaprop-pethyl are the safest to use on red clover underseeded to winter wheat. The remaining herbicides 2,4-D, dicamba/MCPA/mecoprop, dichlorprop/2,4-D, thifensulfuron/ tribenuron + MCPA, fluroxypyr + MCPA, pyrasulfotole/bromoxynil, and prosulfuron + bromoxynil are more injurious, with the last two being the most harmful. By having identified the least damaging herbicides on underseeded red clover in winter wheat and the optimal timing for herbicide application, growers are more likely to adopt this beneficial agronomic practice, save on fertilizer costs and improve soil quality.展开更多
In the present study,genetic diversity and genetic relationship of 11materials including red clover ( Trifolium pretense L.),white clover ( T.repen L.) and alsike clover ( T.hybridum L.) and leaf-type variants of whit...In the present study,genetic diversity and genetic relationship of 11materials including red clover ( Trifolium pretense L.),white clover ( T.repen L.) and alsike clover ( T.hybridum L.) and leaf-type variants of white clover were investigated by SRAP (sequence related amplified polymorphism).Forty SRAP primers were screened,and 792 reliable bands were amplified,among which 426 (53.8%) were polymorphic.The number of polymorphic bands per pair of primers ranged from 3 to 38 with an average of 10.6.SRAP cluster analysis showed that the similarity coefficients between white clover materials were from 0.465 to 0.997 with an average of 0.812,indicating that there was certain genetic diversity among white clover.Specific bands appeared in white clover variants,indicating that there were certain differences in DNA sequence between normal while clover plants and their leaf-type variants.The similarity coefficients between red clover materials were from 0.457 to 0.827 with an average of 0.597,indicating that the different red clover germplasms had genetic diversity at the DNA molecular level.The results also showed that the genetic relationship of the alsike clover with the red clover was closer than that with the white clover.This study shows that SRAP technique can be effectively used for the analysis on interspecific and intraspecific relationship,germplasm resource identification and genetic diversity of clover.展开更多
[Objective] To investigate the effects of fertilization on growth and development of rod clover. [Method] American red clover "Agivasion" was used as tested materials. In field, four treatments were used, including ...[Objective] To investigate the effects of fertilization on growth and development of rod clover. [Method] American red clover "Agivasion" was used as tested materials. In field, four treatments were used, including no fertilization (CK), application with N fertilizer, application with NP fertilizer, and application with NPK fertilizer. And the effects of fertilization on growth and development of red clover were analyzed. [ Result] After fertilization, plant height was increased, particularly in application with NP fertilizer. Branching was promoted, particularly in application with NPK fertilizer. Rhizome became thicker, particularly in application with NP or NPK fertilizer. The fertilization promoted accumulation of dry matter in stems and leaves and increased yield per plant significantly at the 0. 01 level. This promoting effect became weaker in such order: NPK fertilizer 〉 NP fertilizer ≈ N fertilizer 〉 CK. [ Conclusion] Rational fertilization can promote growth and development of red clover.展开更多
Background:Incorporation of legume species into native North American pastures is considered an effective method to increase native pasture productivity and improve the nutritive value of forage.This study evaluated t...Background:Incorporation of legume species into native North American pastures is considered an effective method to increase native pasture productivity and improve the nutritive value of forage.This study evaluated the effects of inclusion of purple prairie clover(PPC,Dalea purpurea Vent.),a native legume forage,with native coolseason grasses on the in vitro fermentation and in situ digestibility of mixed forages.Methods:Whole plant PPC and mixtures of cool-season grasses were harvested when the PPC reached the vegetative(VEG),full flower(FL)and seedpod(SP)stages,and were combined in ratios(DM basis)of 0:100,25:75,50:50,75:25 and 100:0 at each maturity.In vitro ruminal incubations using these mixtures were conducted for 48 h to determine gas production(GP),in vitro DM disappearance(IVDMD),total volatile fatty acids(VFA)and ammonia-N production.Mixtures of forages harvested when the PPC reached the FL stage and 50:50 mixture of forages harvested at VEG,FL and SP stages were incubated in the rumen of three heifers for 0,2,6,12,24,48,72 and 96 h to determine in situ degradabilities of DM,neutral detergent fibre(aNDF)and crude protein(CP).Results:Contents of aNDF and ADF increased(P<0.01),while CP decreased(P<0.001)as PPC matured.Concentrations of extractable condensed tannins in PPC ranked as FL>VEG>SP(P<0.05).Regardless of PPC proportions in the mixture,GP decreased(P<0.05)with increasing PPC maturity.Increasing PPC proportions linearly increased(P<0.001)GP,IVDMD and total VFA at VEG,but linearly decreased(P<0.001)them at SP.Irrespective of PPC maturity,ammonia-N production linearly increased(P<0.01)with increasing proportions of PPC and the concentration was higher(P<0.05)at VEG than at FL and SP stages.Increasing proportion of PPC at either maturity linearly increased(P<0.001)molar percentage of acetate(A)and branched-chain VFA,but linearly decreased(P<0.001)molar percentage of propionate(P),resulting in a linearly increase(P<0.001)in the A:P ratio.Increasing FL PPC in the mixture linearly and quadratically(P<0.01)increased a(soluble fraction),but linearly and quadratically decreased(P<0.01)b(potentially degradable fraction)for DM and aNDF,resulting in linear(P<0.05)and quadratic(P<0.01)increases in DM and aNDF maximum potential degradabilities(a+b).Effective degradabilities of DM and aNDF were also linearly and quadratically increased(P<0.05),and CP was quadratically increased(P<0.05)with increasing FL PPC,with the greatest effective degradability being observed with ratios between 50:50 and 75:25.Ruminal maximum potential degradabilities of DM and aNDF decreased(P<0.001)as the forage matured.Effective degradability of DM ranked as VEG>FL>SP(P<0.001),whereas the effective degradability of aNDF was similar between VEG and FL and both were greater(P<0.01)than SP.Conclusions:Inclusion of vegetative PPC in a mixed forage diet resulted in the greatest digestibility and incorporation of PPC before seedpod stage with native grasses had a positive effect on ruminal fermentation.Effects of PPC on ruminal digestion depend on both the stage of maturity and its proportion in mixed legume-grass pastures.Pastures containing 50%of PPC in full flower stage would likely provide the greatest quality diet to grazing ruminants subject to potential animal selectivity.展开更多
Two field experiments were conducted during two successive winter seasons of 2008/2009 and 2009/2010 to investigate the potentialities of mixing Egyptian clover with ryegrass under bio, organic and mineral fertilizati...Two field experiments were conducted during two successive winter seasons of 2008/2009 and 2009/2010 to investigate the potentialities of mixing Egyptian clover with ryegrass under bio, organic and mineral fertilization treatments and their combination to increase forage yield and quality grown under sandy soil conditions. The experiment included the combination of five mixing ratios (Egyptian clover alone, ryegrass alone, 75% Egyptian clover: 25% ryegrass, 50% Egyptian clover: 50% ryegrass and 25% Egyptian clover: 75% ryegrass) and eight fertilizer sources, which include control, organic fertilization, bio fertilization, chemical fertilizer, organic + bio fertilizer, organic + chemical fertilizer, bio + chemical fertilizer and combination of organic and chemical and bio fertilizers. The obtained results indicate the superiority of 75% E. clover: 25% ryegrass mixture fertilizedby Bio + O + N in fresh and dry forage production. On the other hand, it reported the lowest dry weight of weeds g/m2. Chemical analysis of forage plants showed that the mixture of ?75% E. clover: 25% ryegrass surpassed that of other treatments yield for crude protein, ether extract and ash. The results also revealed that the highest record of DCP, crude fiber and TDNY was obtained by forage mixture of 75% E. clover: 25% ryegrass fertilized with Bio + O + N. Such higher yield of these characters hassecured a balanced ratio which is really needed for ruminants ration.展开更多
Berseem clover (Trifolium alexandrinum L.) is the main winter forage legume in Egypt. Despite that the yield and protein content of berseem clover are high, it is characterized by low dry matter content especially in ...Berseem clover (Trifolium alexandrinum L.) is the main winter forage legume in Egypt. Despite that the yield and protein content of berseem clover are high, it is characterized by low dry matter content especially in the 1st cut. Intercropping berseem clover with forage grasses, especially annual ryegrass (Lolium multiflorum Lam.) is a recommended technique to produce higher forage yield with better balanced nutritional quality. However, little is known about the performance of these proposed mixtures under the arising organic farming system in Egypt. Organic farming and the application of organic fertilizers (compost and poultry manure) are receiving increased attention in the last few years. Thus, the current study was carried out on the winter season of two successive years (2012 and 2013) in the experimental station of SEKEM organic farm, Egypt. Main aim was to investigate the variation in 1st cut forage yield and nutritive value of berseem clover and annual ryegrass, grown in pure stands and with three mixing rates, under three organic fertilization regimes (compost, poultry litter, and no fertilization). Nutritive value was judged through investigating the crude protein (CP), total carbohydrates (TC), and fiber fractions (NDF, ADF, and ADL). Results revealed significant two-way interaction between the forage treatments and the fertilization regimes for all the studied parameters. In general, mixing berseem clover with annual ryegrass, fertilized by compost or poultry litter resulted in significantly higher forage yield, dry matter content, and balanced CP, TC, and fiber fractions, compared to the pure stands.展开更多
Proper mineral nutrition of red clover especially on the acid soils is a prerequisite for the realization of maximum potential for seed yield. The field experiment with four cultivars of red clover were established on...Proper mineral nutrition of red clover especially on the acid soils is a prerequisite for the realization of maximum potential for seed yield. The field experiment with four cultivars of red clover were established on soil having a pH (in H2O) of 4.8, in order to in conditions of dense planting (20 cm inter row) analyzes the effect of foliar application of phosphorus and potassium (PK) on yield and yield components (number of stems/m2, number of inflorescences/m2, number of inflorescences/stem, number of flowers/inflorescence, number of seeds/inflorescence and one thousand grain weight). Regardless of foliar treatment with PK, varieties differed in the number of inflorescences/m2, number of flowers/inflorescence, number of seeds/inflorescence and seed yield. Foliar application of PK in the phase of intensive growth of red clover had a positive impact on number of stem/m2, number of inflorescences/m2 and seed yield in all varieties, as well as on the number of flowers/inflorescence, number of seeds/inflorescence in cultivar Viola.展开更多
基金Science&Technology Specific Project for Enriching People and Strengthening County Economy(BN2015221)~~
文摘In order to develop organic rice and increase paddy soil fertility by cloverorganic rice rotation, the effects of N, P and K fertilizers on growth of clover nitrogen-fixing rhizobia and soil fertility after plantation were investigated, thereby providing certain reference for reasonable cultivation of clover and improvement of soil fertility. A two-year experiment was conducted from 2012 to 2013. The clover was cultivated after rice every year, and different levels of N, P and K fertilizers were applied before winter. In the treatment group, no fertilizer was applied. The effects of different fertilizers and different application amounts on clover yield, nitrogen-fixing rhizobia quantity, nitrogen-fixing rhizobia weight and soil fertility after plantation were analyzed. The results showed that the application of N, P and K fertilizers in the early stage had significantly effect on the growth of clover. When the application amount of N fertilizer was 75 kg/hm^2(N 46%), the clover yield, nitrogen-fixing rhizobia quantity and nitrogen-fixing rhizobia weight were highest. The soil nitrogen content after plantation increased with the increase of the application amount of N fertilizer, while the P and K content decreased and then increased with the increased application amounts. Soil available P content increased with the increased application amounts of N and P fertilizers, but it did not change significantly with the increased application amount of K fertilizer. Soil available K content increased first and then decreased with the increased application amounts of N and P fertilizers.When the application amounts of N and P fertilizers were 150(N 46%) and 300(P_2O_5 12%) kg/hm^2, soil available K content reached the maximum. Soil organic matter content increased with the increased application amounts of N, P and K fertilizers. Therefore, in the cultivation of clover, appropriate application of N, P and K fertilizers in the early stage can improve clover yield and soil fertility.
基金Supported by National Key Technology R & D Program(2006BAD16B07) Fund of Science and Technology in Guizhou Province ([2008]2074)~~
文摘[Objective] The study aimed to explore the degradation law and trend of artificial grassland. [Method] Taking the ryegrass (Lolium perenne) - white clover ( Trifolium repens) artificial grassland in Maiping Township, Guizhou Province as the research object, the grassland vegetation of 40 quadrate from different areas (area around the sheep shed, hilltop, hillside, flatland at the foot of the hill) were analyzed by comparing the dominance and richness index. [ Result] Degradation of different degrees appeared in various areas of this artificial grassland. To be specific, the degradation of grassland around the sheep shed was not serious for less consumption by the sheep; however, the areas on the hilltop and hillside degenerated to the grassland types of dallis grass ( Paspalum dilatatum Poir. ) -Indian lovegrass ( Eragrostis pilosa) and dallis grass - cogongrass [ Imperata cylindrica ( Linn. ) Beauv. ], respectively, and the area at the foot of the hill degenerated to the grassland type dominated by garland chrysanthetnum ( Chrysanthemum coronarium L. ) and knotgrass ( Paspalum distichum L. ). [ Conclusion ] This study provided a basis for grassland improvement as well as the efficient and sustainable utilization of grazing-type artificial grassland in South China.
基金National Key Basic Research Support Foundation (NKBRSF) of China(No. G1999011807) the Joint Open Laboratory of Soil and Environment between the Institute of Soil Science of the Chinese Academy of Sciences and Hong Kong Baptist University.
文摘The capacities of two arbuscular mycorrhizal (AM) fungi, Glomusmosseae and Glomus versiforme, to mineralize added organic P werestudied in a sterilized clacareous soil. Mycorrhizal (inoculated witheither of the AM fungi) and non-mycorrhizal red clover (Trifoliumpratense L.) plants were grown for eight weeks in pots with upperroot, central hyphal and lower soil compartments. The hyphal and soilcompartments received either organic P (as Na-phytte) or inorganic P(as KH_2PO_4) at the rate of 50 mg P kg^-1. No P was added to theroot compartments.
基金Project supported by the National Natural Science Foundation of China(Nos.40432004 and 20677015)the Postdoctoral Science Foundation of China(No.20070420094)+2 种基金the Postdoctoral Science Foundation of Shanghai Municipality,China(No.08R214116)the Science and Technology Commission of Shanghai Municipality,China(No.0752nm025)theNational High-Tech Research and Development Program(No.2007AA06Z331)
文摘Phytoremediation has been used as an emerging technology for remediation of soil contamination with polycyclic aromatic hydrocarbons(PAHs),ubiquitous persistent environmental pollutants derived from natural and anthropogenic processes,in the last decade.In this study,a pot experiment was conducted to investigate the potential of phytoremediation of pyrene from spiked soils planted with white clover(Trifolium repens)in the greenhouse with a series of pyrene concentrations ranging from 4.22 to 365.38 mg kg-1.The results showed that growth of white clover on pyrenecontaminated soils was not affected.The removal of pyrene from the spiked soils planted with white clover was obviously higher than that from the unplanted soils.At the end of the experiment(60 d),the average removal ratio of pyrene in the spiked soils with white clover was 77%,which was 31%and 57%higher than those of the controls with or without micobes, respectively.Both roots and shoots of white clover took up pyrene from the spiked soils and pyrene uptake increased with the soil pyrene concentration.However,the plant-enhanced dissipation of soil pyrene may be the result of plant-promoted microbial degradation and direct uptake and accumulation of pyrene by white clover were only a small part of the pyrene dissipation.Bioconcentration factors of pyrene(BCFs,ratio of pyrene,on a dry weight basis,in the plant to that in the soil)tended to decrease with increase in the residual soil pyrene concentration.Therefore,removal of pyrene in the contaminated soils was feasible using white clove.
基金financial support of the Key Laboratory of Grassland Ecosystem of Ministry of Education of China(CYZS-2011006)
文摘Globally, white clover(Trifolium repens L.) is commonly infected by plant viruses. It is grown at gardens, roadsides, and public areas as ornamental plants in northern China. Some leaves present disease symptoms that are similar to those of virus infection. However, to our knowledge, no records are available from China regarding white clover(Trifolium repens L.) virus co-infection. To determine the viral species that infect white clover in China, plant samples with virus disease symptoms were collected and virion morphology and ultrastructure morphology of co-infected plants were observed by electron microscopy; viruses were detected by enzyme linked immunosorbent assay(ELISA) and reverse transcription PCR(RT-PCR). Virus co-infection was studied by double antibody sandwich enzyme linked immunosorbent assay(DAS-ELISA) and real-time fluorescence quantitative PCR(RT-q PCR) after rub-inoculation of virus-free white clover planted in a growth chamber(25°C) with a photoperiod of 16 h, using single or several purified virions. Results showed that there were six types of symptoms, including those of shrinking mosaic, shrinking and macular mosaic, severe mottle mosaic, yellow macular mosaic, shrinkage chlorisis, and ring plaque and ring stria. The incidence rates for each symptom were 20.93, 1.48, 16.85, 59.07, 1.30, and 0.37%, respectively, based on the field investigation. Two types of viral pathogens were identified as Alfalfa mosaic virus(AMV) and White clover mosaic virus(WCMV). In mesophyll cells, virus particles with bacilliform virions formed aggregates and linear virions were bundle shaped. The detection rate of AMV was 100% in white clover samples by DAS-ELISA and RT-PCR detection, whereas that of WCMV was 83.33%. The co-infection rate was 83.33%. The relative contents of AMV and WCMV were significantly increased by 5.897-and 3.515-fold upon co-infection, when compared to that with single virus infection. We observed larger starch particles and fewer or collapsed chloroplast grana in co-infected plants; in addition, vacuoles were twisted and smaller, compared to those of healthy plants. To our knowledge, this is the first report of co-infection by AMV and WCMV in white clover, which has caused severe mosaicism and ultrastructure lesions in co-infected plants in China.
文摘In December of 3 years, 87 beef cows with nursing calves (.594 ± 9.8 kg; calving season, September to November) at side were stratified by body condition score, body weight, cow age, and calf gender and divided randomly into 6 groups assigned to 1 of 6 cool-season annual pastures (0.45 ha/cow) that had been interseeded into a dormant common bermudagrass (Cynodon dactylon [L.] Pers.)/bahiagrass (Paspalum notatum Flugge) sod. Pastures contained 1 of the following 3 seeding mixtures (2 pastures/mixture): 1) wheat (Triticum aestivurn L.) and ryegrass (Lofium multiflorum Lain., WRG), 2) wheat and ryegrass plus red clover (Trifolium pretense L, WRR), or 3) wheat and ryegrass plus white (Trifofium repens L.) and crimson clovers (Trifolium incarnatum L., WRW). All groups had ad libitum access to grass hay (12% crude protein; 58% total digestible nutrients). The second week in December, cow estrous cycles were synchronized and artificially inseminated. In late December, a bull was placed with each group for 60-d. Data were analyzed with an analysis of variance using a mixed model containing treatment as the fixed effect and year as the random effect. Body weight and condition scores did not differ (P ≥ 0.27) among cows between February and June. Calf birth weights or average daily gain did not differ (P≥ 0.17) among treatments; however, calves grazing pastures with clovers did tend (P= 0.06) to weigh more than calves grazing grass only. Weaning weight per cow exposed to a bull was greater (P= 0.02) for WRR and WRW than WRG. Cows grazing winter-annual pastures containing clovers tended to wean more calf body weight per cow exposed to a bull than cows grazing the grass only pastures.
文摘The effect of die inlet and transition geometry on the extrusion loads and ~aaterial flow for extrusion of clover sections were investigated and presented both theoretically and experimentally. For this purpose, four different die geometries including straight tapered and cosine transition profile and each of them having round and clover inlet geometries were chosen. In the experimental study, commercially pure lead was used because of its hot forming characteristic at room temperature. A newly kinematical admissible velocity field to analyze different profiles of extrusion dies of clover section from round bars was proposed by upper bound analysis. It is clear that the extrusion loads obtained from the theoretical analysis for various die inlet-die transition geometry combinations are in good agreement with the experimental results. Axis deviations of the parts which define the dimensional quality of the products were also investigated.
基金Project(2011-0017407)supported by the National Research Foundation of Korea(NRF)funded by Korea government(MEST)
文摘Laser assisted turning (LAT) is one of the advanced machining technologies, which uses laser power to heat the surface of a workpiece before the material is removed. It has several advantages of low manufacturing costs, high productivity and high qualities to machine difficult-to-cut materials such as silicon nitride, muUite, zirconia and Ni. A large part of studies on LAT have been focused on a round bar. With increasing demands for high quality products and high performance engineering system, the researches on LAT for clover and square section members are necessary. But, these workpieces are impossible to be machined on conventional CNC lathe and to generate NC code with current CAM softwares. As a basic research for combining LAT with a tilting index table type 5-axis machining center, i.e. laser assisted turn-mill, a new method is suggested to generate NC code that can process various types of clover and square section members through development of C++ program.
基金Supported by the National Natural Science Foundation of China(31802120)the Natural Science Foundation of Heilongjiang Province of China(LH2021C040)Academic Backbone Fund Project of Northeast Agricultural University。
文摘Although white clover has been widely studied and applied,less is known about its physiological responses to chilling stress in northern China.The effects of acclimation time of chilling stress in the two white clover cultivars,Rivendel and Haifa,after exposure to 4℃for 0,4,8,12,24 and 48 h were investigated.Seven physiological indexes were measured in shoots of the two cultivars.It was found that Rivendel had a strong ability to maintain the stability of cells,cope with oxidative damage and accumulate osmotic adjustment substances compared with Haifa.In addition,a comprehensive evaluation on the chilling tolerance of the two cultivars based on subordinate function value analysis showed a stronger chilling tolerance of Rivendel than Haifa.
文摘Effect of seeding rate mixture from Egyptian clover (Trifolium alexandrinum L.) E.C. and barley (Hordum vulgare L.) B, N-fertilization levels and their interaction on quantity and quality of forage yield. Two field experiments were conducted during winter season of 2012/2013 and 2013/2014 in sandy soil at research and production station of National Research Centre, Al Nubaria district, Al Behaira Governorate, Egypt. The experiment included two factors first was five mixtures from seeding rates (24 kg EC/fed. – 50 kg B/fed. – 18 kg EC + 12.5 kg B/fed. – 12kg EC + 25 kg B/fed. – 6 kg EC + 37.5 kg B/fed.) and second factor was three N levels (30-45-60 kg N/fed.). Two cuts were obtained at 60 and 100 DAS. Results indicated significant differences between mixture rates for all studied characters in both cuts 60 and 100 DAS. Significant differences between N levels for all studied characters at 60 DAS, also, forage yield /fed. and DM% at 100 DAS. It can be introducing forage mixture of 18 kg EC + 12.5 B had balanced character in forage yield/fed., DM%, carbohydrate % and protein % at both cuts 60 and 100 DAS.
文摘Agroforestry systems have the potential to provide year-long income opportunities via the integrated forage or crop, timber, and livestock. Legumes are an attractive alternative option during the growing season when more traditional forages may not be as productive. The objective of this study was to test the establishment of arrowleaf and white clover grown under varying pine tree alley widths. In 2011, existing forage was removed in 15-yr old loblolly pine tree row alleys of different widths (3.7, 4.9, 7.3, and 9.8 m), including an open area. Arrowleaf, as an annual, was replanted in 2012. Seedlings were counted twice/year, while dry matter was measured three times/year. Photosynthetically active radiation (PAR) was measured in all alley widths to compare light penetration through the canopy. Hot and dry conditions occurred throughout 2012, affecting results. In 2012 and 2013, the greatest PAR for most treatments was observed in June. Seedling counts for all treatments were greatest immediately after establishment, and gradually declined throughout the course of the study. Dry matter yields increased throughout the growing season, and were greatest in arrowleaf clover in the open area on all measurement dates;however, increased weed pressure and repeated flooding affected yields. This study demonstrated that clover establishment in shady wooded areas is possible, but only under suitable environmental conditions.
基金funded in part by the Grain Farmers of Ontario,the Agricultural Adaptation Council(CanAdvance Program)and the Ontario Ministry of Agriculture,Food and Rural Affairs.
文摘Underseeding red clover in winter wheat is a beneficial agronomic practice. Still, many growers tend to forgo this approach. One reason is that herbicides used on winter wheat may injure underseeded red clover, reducing its biomass and the subsequent benefits it provides. Therefore, the effect of winter wheat herbicides on underseeded red clover needs to be evaluated. The objectives of this research were to assess the crop tolerance of underseeded red clover to ten winter wheat herbicides used in Ontario, Canada and determine if red clover tolerance differed when the herbicides were applied at various winter wheat growth stages. Experiments were conducted in 2009 and 2010 at four different Ontario locations. Each herbicide treatment was either applied at an early, normal or late timing. Overall, red clover was not affected by herbicides applied at the early timing. The likelihood of herbicides causing injury and reducing biomass of underseeded red clover increased when they were applied at the more advanced winter wheat growth stages. If timing is a constraint, the three herbicides bromoxynil/MCPA, tralkoxydim, and fenoxaprop-pethyl are the safest to use on red clover underseeded to winter wheat. The remaining herbicides 2,4-D, dicamba/MCPA/mecoprop, dichlorprop/2,4-D, thifensulfuron/ tribenuron + MCPA, fluroxypyr + MCPA, pyrasulfotole/bromoxynil, and prosulfuron + bromoxynil are more injurious, with the last two being the most harmful. By having identified the least damaging herbicides on underseeded red clover in winter wheat and the optimal timing for herbicide application, growers are more likely to adopt this beneficial agronomic practice, save on fertilizer costs and improve soil quality.
基金Supported by Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2016A02-1)China Agriculture Research System-Green Manure(CARS-22)Young Talents Training Program of Shandong Academy of Agricultural Sciences(2018-2020)
文摘In the present study,genetic diversity and genetic relationship of 11materials including red clover ( Trifolium pretense L.),white clover ( T.repen L.) and alsike clover ( T.hybridum L.) and leaf-type variants of white clover were investigated by SRAP (sequence related amplified polymorphism).Forty SRAP primers were screened,and 792 reliable bands were amplified,among which 426 (53.8%) were polymorphic.The number of polymorphic bands per pair of primers ranged from 3 to 38 with an average of 10.6.SRAP cluster analysis showed that the similarity coefficients between white clover materials were from 0.465 to 0.997 with an average of 0.812,indicating that there was certain genetic diversity among white clover.Specific bands appeared in white clover variants,indicating that there were certain differences in DNA sequence between normal while clover plants and their leaf-type variants.The similarity coefficients between red clover materials were from 0.457 to 0.827 with an average of 0.597,indicating that the different red clover germplasms had genetic diversity at the DNA molecular level.The results also showed that the genetic relationship of the alsike clover with the red clover was closer than that with the white clover.This study shows that SRAP technique can be effectively used for the analysis on interspecific and intraspecific relationship,germplasm resource identification and genetic diversity of clover.
基金supported by the Returning Land for Farming to Forestry Project in Hunan Province
文摘[Objective] To investigate the effects of fertilization on growth and development of rod clover. [Method] American red clover "Agivasion" was used as tested materials. In field, four treatments were used, including no fertilization (CK), application with N fertilizer, application with NP fertilizer, and application with NPK fertilizer. And the effects of fertilization on growth and development of red clover were analyzed. [ Result] After fertilization, plant height was increased, particularly in application with NP fertilizer. Branching was promoted, particularly in application with NPK fertilizer. Rhizome became thicker, particularly in application with NP or NPK fertilizer. The fertilization promoted accumulation of dry matter in stems and leaves and increased yield per plant significantly at the 0. 01 level. This promoting effect became weaker in such order: NPK fertilizer 〉 NP fertilizer ≈ N fertilizer 〉 CK. [ Conclusion] Rational fertilization can promote growth and development of red clover.
基金This study was funded from AAFC/Canadian Beef Cattle Industry Science ClusterAlberta Livestock and Meat Agency.This is Lethbridge Research and Development Centre contribution number 38717038.
文摘Background:Incorporation of legume species into native North American pastures is considered an effective method to increase native pasture productivity and improve the nutritive value of forage.This study evaluated the effects of inclusion of purple prairie clover(PPC,Dalea purpurea Vent.),a native legume forage,with native coolseason grasses on the in vitro fermentation and in situ digestibility of mixed forages.Methods:Whole plant PPC and mixtures of cool-season grasses were harvested when the PPC reached the vegetative(VEG),full flower(FL)and seedpod(SP)stages,and were combined in ratios(DM basis)of 0:100,25:75,50:50,75:25 and 100:0 at each maturity.In vitro ruminal incubations using these mixtures were conducted for 48 h to determine gas production(GP),in vitro DM disappearance(IVDMD),total volatile fatty acids(VFA)and ammonia-N production.Mixtures of forages harvested when the PPC reached the FL stage and 50:50 mixture of forages harvested at VEG,FL and SP stages were incubated in the rumen of three heifers for 0,2,6,12,24,48,72 and 96 h to determine in situ degradabilities of DM,neutral detergent fibre(aNDF)and crude protein(CP).Results:Contents of aNDF and ADF increased(P<0.01),while CP decreased(P<0.001)as PPC matured.Concentrations of extractable condensed tannins in PPC ranked as FL>VEG>SP(P<0.05).Regardless of PPC proportions in the mixture,GP decreased(P<0.05)with increasing PPC maturity.Increasing PPC proportions linearly increased(P<0.001)GP,IVDMD and total VFA at VEG,but linearly decreased(P<0.001)them at SP.Irrespective of PPC maturity,ammonia-N production linearly increased(P<0.01)with increasing proportions of PPC and the concentration was higher(P<0.05)at VEG than at FL and SP stages.Increasing proportion of PPC at either maturity linearly increased(P<0.001)molar percentage of acetate(A)and branched-chain VFA,but linearly decreased(P<0.001)molar percentage of propionate(P),resulting in a linearly increase(P<0.001)in the A:P ratio.Increasing FL PPC in the mixture linearly and quadratically(P<0.01)increased a(soluble fraction),but linearly and quadratically decreased(P<0.01)b(potentially degradable fraction)for DM and aNDF,resulting in linear(P<0.05)and quadratic(P<0.01)increases in DM and aNDF maximum potential degradabilities(a+b).Effective degradabilities of DM and aNDF were also linearly and quadratically increased(P<0.05),and CP was quadratically increased(P<0.05)with increasing FL PPC,with the greatest effective degradability being observed with ratios between 50:50 and 75:25.Ruminal maximum potential degradabilities of DM and aNDF decreased(P<0.001)as the forage matured.Effective degradability of DM ranked as VEG>FL>SP(P<0.001),whereas the effective degradability of aNDF was similar between VEG and FL and both were greater(P<0.01)than SP.Conclusions:Inclusion of vegetative PPC in a mixed forage diet resulted in the greatest digestibility and incorporation of PPC before seedpod stage with native grasses had a positive effect on ruminal fermentation.Effects of PPC on ruminal digestion depend on both the stage of maturity and its proportion in mixed legume-grass pastures.Pastures containing 50%of PPC in full flower stage would likely provide the greatest quality diet to grazing ruminants subject to potential animal selectivity.
文摘Two field experiments were conducted during two successive winter seasons of 2008/2009 and 2009/2010 to investigate the potentialities of mixing Egyptian clover with ryegrass under bio, organic and mineral fertilization treatments and their combination to increase forage yield and quality grown under sandy soil conditions. The experiment included the combination of five mixing ratios (Egyptian clover alone, ryegrass alone, 75% Egyptian clover: 25% ryegrass, 50% Egyptian clover: 50% ryegrass and 25% Egyptian clover: 75% ryegrass) and eight fertilizer sources, which include control, organic fertilization, bio fertilization, chemical fertilizer, organic + bio fertilizer, organic + chemical fertilizer, bio + chemical fertilizer and combination of organic and chemical and bio fertilizers. The obtained results indicate the superiority of 75% E. clover: 25% ryegrass mixture fertilizedby Bio + O + N in fresh and dry forage production. On the other hand, it reported the lowest dry weight of weeds g/m2. Chemical analysis of forage plants showed that the mixture of ?75% E. clover: 25% ryegrass surpassed that of other treatments yield for crude protein, ether extract and ash. The results also revealed that the highest record of DCP, crude fiber and TDNY was obtained by forage mixture of 75% E. clover: 25% ryegrass fertilized with Bio + O + N. Such higher yield of these characters hassecured a balanced ratio which is really needed for ruminants ration.
文摘Berseem clover (Trifolium alexandrinum L.) is the main winter forage legume in Egypt. Despite that the yield and protein content of berseem clover are high, it is characterized by low dry matter content especially in the 1st cut. Intercropping berseem clover with forage grasses, especially annual ryegrass (Lolium multiflorum Lam.) is a recommended technique to produce higher forage yield with better balanced nutritional quality. However, little is known about the performance of these proposed mixtures under the arising organic farming system in Egypt. Organic farming and the application of organic fertilizers (compost and poultry manure) are receiving increased attention in the last few years. Thus, the current study was carried out on the winter season of two successive years (2012 and 2013) in the experimental station of SEKEM organic farm, Egypt. Main aim was to investigate the variation in 1st cut forage yield and nutritive value of berseem clover and annual ryegrass, grown in pure stands and with three mixing rates, under three organic fertilization regimes (compost, poultry litter, and no fertilization). Nutritive value was judged through investigating the crude protein (CP), total carbohydrates (TC), and fiber fractions (NDF, ADF, and ADL). Results revealed significant two-way interaction between the forage treatments and the fertilization regimes for all the studied parameters. In general, mixing berseem clover with annual ryegrass, fertilized by compost or poultry litter resulted in significantly higher forage yield, dry matter content, and balanced CP, TC, and fiber fractions, compared to the pure stands.
文摘Proper mineral nutrition of red clover especially on the acid soils is a prerequisite for the realization of maximum potential for seed yield. The field experiment with four cultivars of red clover were established on soil having a pH (in H2O) of 4.8, in order to in conditions of dense planting (20 cm inter row) analyzes the effect of foliar application of phosphorus and potassium (PK) on yield and yield components (number of stems/m2, number of inflorescences/m2, number of inflorescences/stem, number of flowers/inflorescence, number of seeds/inflorescence and one thousand grain weight). Regardless of foliar treatment with PK, varieties differed in the number of inflorescences/m2, number of flowers/inflorescence, number of seeds/inflorescence and seed yield. Foliar application of PK in the phase of intensive growth of red clover had a positive impact on number of stem/m2, number of inflorescences/m2 and seed yield in all varieties, as well as on the number of flowers/inflorescence, number of seeds/inflorescence in cultivar Viola.