PPARγ上调对PCOS患者卵巢颗粒细胞P450arom mRNA的表达及T转化效应的影响

目的:探讨PPARγ上调对PCOS患者卵巢颗粒细胞P450arom mRNA表达的调控作用及对T转化效应的影响。方法:提取10例雄激素正常的PCOS患者(NA-PCOS组)、10例伴高雄激素血症的PCOS患者(HA-PCOS组)及10例仅因输卵管因素不孕患者(对照组)的卵巢颗粒细胞,分别给予0、1、10 nmol/L的PPARγ激动剂罗格列酮培养48 h后,采用RT-PCR测定PPARγ、P450arom mRNA的表达,采用ELISA法测定培养液中E2值,计算T转化率。结果:PPARγ上调后,3组患者P450arom mRNA的表达均降低,T转化率下降,尤其是HA-PCOS组(P<0.05)。PPARγ上调(10 nmol/L罗格列酮处理)后,HA-PCOS组卵巢颗粒细胞中PPARγmRNA的表达水平和T转化率负相关,P450arom mRNA的表达水平和T转化率正相关(r=-0.633、0.829,P<0.05)。结论:PPARγ可能通过抑制P450arom参与PCOS高雄激素血症的发生。

T305氧化锌脱硫剂脱除COS宏观动力学研究

用热重法研究了球形及圆柱体T305氧化锌脱硫剂脱除COS反应的宏观动力学.实验表明,该过程对COS为一级反应.在240—380℃的温度范围内,反应初期受反应本身控制,中后期转移到颗粒扩散控制.动力学行为可用推荐的等效粒子模型描述.给出该模型的各参数估值及圆柱体形状因子.

不同剂量异烟肼治疗肺结核的临床效果及对患者肺功能和T淋巴细胞亚群的影响

目的 探讨不同剂量异烟肼治疗肺结核的临床效果及对患者肺功能、T淋巴细胞亚群的影响。方法 选取2020年6月至2022年6月河北省胸科医院收治的肺结核患者90例为研究对象,按照随机数字表法分为对照组和观察组,各45例。对照组在常规抗结核药物基础上予0.3 g/d异烟肼治疗,观察组在常规抗结核药物基础上予0.5 g/d异烟肼治疗,2组疗程均为6个月。比较2组临床疗效,评估患者肺功能及T淋巴细胞亚群变化情况。结果 观察组总有效率高于对照组(P<0.05)。治疗后,2组最大呼气流量、第1秒用力呼气容积均高于治疗前,且观察组均高于对照组[(7.8±1.4)L/s比(6.3±1.2)L/s、(1.48±0.28)L比(1.15±0.23)L](均P<0.05)。治疗后,2组血清CD+3、CD+4、CD+4/CD+8比值均高于治疗前,且观察组均高于对照组,CD+8均低于治疗前,且观察组低于对照组(均P<0.05)。2组不良反应发生率比较差异无统计学意义(P>0.05)。结论 不同剂量异烟肼治疗肺结核的临床效果不同,增加异烟肼剂量至0.5 g/d能够提升疗效,促进肺结核患者肺功能改善,提高免疫功能,且安全性较好。

以眼部肿物为首发表现的鼻型结外NK/T细胞淋巴瘤的临床分析

目的 比较以眼部肿物为首发表现的鼻型结外NK/T细胞淋巴瘤(extranodal NK/T-cell lymphoma,nasal type,ENKTCL),与其他部位首发的ENKTCL临床特征、疗效及预后的差异。方法 回顾性分析2009年5月至2021年2月于首都医科大学附属北京同仁医院血液科收治的266例新诊断且未经治疗的ENKTCL患者的临床资料,结合随访调查,对两组患者的临床特征、治疗后缓解率及生存情况进行比较分析。结果 266例ENKTCL患者中,26例患者以眼部肿物为首发表现,其中眼睑肿胀为最常见的临床表现。与其他ENKTCL组相比,眼部ENKTCL组中Ⅲ/Ⅳ期患者所占比例更高(23.8%vs 42.3%,P=0.0394),LDH升高患者的比例更高(27.1%vs 46.2%,P=0.0417)。预后方面,眼部ENKTCL组KPI评分≥3分的患者比例高于其他ENKTCL组(46.2%vs 21.3%,P=0.0046);眼部ENKTCL组PINK评分为高危组的患者比例高于其他ENKTCL组(38.5%vs 19.2%,P=0.0219)。疗效方面,两组患者在治疗方案选择上差异无统计学意义;眼部ENKTCL组评效为疾病进展的患者比例高于其他ENKTCL患者组(46.2%vs 22.9%,P=0.0090)。生存方面,眼部ENKTCL组中死亡患者共有12(46.2%)例,其他ENKTCL组中有56(23.3%)例。两组患者3年及5年的PFS率差异无统计学意义,但眼部和其他ENKTCL组3年的OS率分别为69.2%和76.7%,以及5年的OS率分别为58.6%和72.2%,差异有统计学意义(P=0.0321)。结论 以眼部肿物为首发临床表现的ENKTCL具有高肿瘤负荷、疾病进展快、对治疗的反应不佳及预后差等特点,及时进行影像学检查及组织活检对明确诊断尤为重要。

CAR-T细胞治疗在儿童中的毒副作用

嵌合抗原受体(CAR)-T细胞因其特异性识别功能及细胞毒性,已成为治疗恶性肿瘤最有前途的方法之一,但它也具有一系列的毒副作用,特别是对儿童,毒性反应发展迅速,严重者还会危及生命。本文对CAR-T细胞治疗在儿童中的毒副作用,及其临床表现和治疗等方面进行概述,旨在优化其在儿童中的应用。

房地产股价波动对高技术板块的溢出效应研究——基于DGC-t-MSV模型

过度的金融资产投资可能导致房地产股价泡沫,并且通过风险溢出渠道给高技术板块带来价格冲击。研究聚焦于房地产行业和高技术产业的股票价格指数,结合MODWT分解和DGC-t-MSV模型进行实证分析,结果表明:其一,“房地产-高技术”的股票价格动态相关,房地产行业价格波动的传导作用更加强烈,对高技术板块具有明显的溢出效应;其二,实施限制性政策后房地产行业的挤出效应降低,高技术及其分类产业的创新投入受到的限制逐渐减弱;其三,短、中期频繁交易是导致房地产溢价的主要原因,需要警惕高技术板块面临的溢出风险。积极引导高技术板块的价值投资,合理配置技术要素,能够有效促进高技术产业的高质量、高水平发展。

爆炸载荷作用下钢筋混凝土预应力T梁桥毁伤模式研究

目的研究不同打击模式下钢筋混凝土预应力T梁桥的毁伤效应,为钢筋混凝土预应力T梁桥的毁伤评估及打击决策提供参考。方法通过数值仿真研究某弹药装药空中爆炸、接触爆炸和内爆炸3种打击模式下,钢筋混凝土预应力T梁桥的毁伤效应。结果不同打击模式下钢筋混凝土预应力T梁桥的毁伤模式差异较大:空中爆炸和接触爆炸桥梁主要发生桥面破孔及塑性变形;内爆炸桥梁除桥面发生层裂破碎外,T梁腹板及横隔梁也发生外凸变形、混凝土崩塌破坏等。结论半密闭空间内爆炸T梁桥的毁伤效果要优于桥面接触爆炸和空中爆炸。

血清vWF、Treg相关细胞因子在急性白血病患儿危险分层及疗效评估中的价值

目的探究血清血管性血友病因子(vWF)、调节性T细胞(Treg)相关细胞因子[转化生长因子-β(TGF-β)、白介素-10(IL-10)、白介素-35(IL-35)]在急性白血病(AL)患儿危险分层及疗效评估中的价值,为AL患儿临床诊疗提供参考。方法选取2020年2月至2022年8月郑州大学第三附属医院收治的78例AL患儿,根据CCLG-ALL-2018方案危险分层分为低危组(39例)、中危组(21例)、高危组(18例)3个亚组,分别比较不同类型、不同危险分层患儿血清vWF、TGF-β、IL-10、IL-35水平,并进行相关性分析。AL患儿均接受规范化治疗,根据治疗1 a疗效评估结果分为临床有效组(完全缓解+部分缓解,62例)、临床无效组(未缓解,16例),分别比较两组治疗2、6个月血清vWF、TGF-β、IL-10、IL-35水平,分析其对AL患儿疗效的预测价值。结果低危组血清vWF、IL-10、IL-35水平低于中危组,中危组血清vWF、IL-10、IL-35水平低于高危组(P<0.05);低危组血清TGF-β水平高于中危组,中危组血清TGF-β水平高于高危组(P<0.05);Spearman相关系数法分析显示,血清vWF、IL-10、IL-35与AL患儿危险分层呈正相关,血清TGF-β与AL患儿危险分层呈负相关(P<0.05);78例AL患儿经1 a治疗后有28例完全缓解、34例部分缓解,计入临床有效组(62例),16例未缓解,计入临床无效组(16例)。治疗2、6个月临床有效组血清vWF、IL-10、IL-35水平均低于临床无效组,血清TGF-β水平高于临床无效组,差异有统计学意义(P<0.05);治疗2、6个月各血清指标联合预测AL患儿临床无效的曲线下面积分别为0.875、0.933(P<0.05)。结论血清vWF、TGF-β、IL-10、IL-35与AL患儿危险分层及疗效有关,动态监测血清vWF、Treg相关细胞因子有利于病情及疗效评估。

Radiation-induced Bystander Effect in Immune Response

Objective Since most reports on bystander effect have been only concerned with radiation-induced damage, the present paper aimed at disclosing whether low dose radiation could induce a stimulatory or beneficial bystander effect. Methods A co-culture system containing irradiated antigen presenting cells (J774A.1) and unirradiated T lymphocytes (EL-4) was established to observe the effect of J774A.1 cells exposed to both low and high doses of X-rays on the unirradiated EL-4 cells. Incorporation of 3H-TdR was used to assess the proliferation of the EL-4 cells, expression of CD80/86 and CD48 on J774A.1 cells was measured with immunohistochemistry and flow cytometry, respectively. NO release from J774A.1 cells was estimated with nitrate reduction method. Results Low dose-irradiated J774A.1 cells could stimulate the proliferation of the unirradiated EL-4 cells while the high dose-irradiated J774A.1 cells exerted an inhibitory effect on the proliferation of the unirradiated EL-4 cells. Preliminary mechanistic studies illustrated that the differential changes in CD48 expression and NO production by the irradiated J774A.1 cells after high and low dose radiation might be important factors underlying the differential bystander effect elicited by different doses of radiation. Conclusion Stimulatory bystander effect can be induced in immune cells by low dose radiation.

衍生T形槽干气密封稳态性能研究

目的 设计具备双向旋转特点的动压型干气密封端面结构,并展现良好的开启性能,以T形槽为优化对象,衍生出一种新型槽型结构。方法 借助有限元软件,计算并对比经典T形槽与衍生槽型在流场特性和稳态性能方面的差异,探讨不同参数对2种槽型干气密封稳态性能的影响规律,揭示衍生槽型的密封机理。结果 衍生槽型的开启力高于经典T形槽,泄漏率也会增加。随着工况参数的升高,2种槽型的稳态性能参数近似线性增加,且差异逐渐增大,最小差异达到6.6%。随着槽深的增加,泄漏率方面的差距在逐渐减小,为扩大衍生槽型的控漏效果,槽深应>5μm。开设有引流槽型结构的衍生槽型稳态性能顺序为发散型>直口型>收敛型,但在文中的工况下,数值差别较小,最大仅为1.2%。结论 相比于经典T形槽,衍生槽型具有更强的动压效应,开启性能明显提升。另外,通过开设不同形式的引流槽可以改变气膜的流动特性和干气密封性能。

Pressure Effects on Spectra of Tunable Laser Crystal GSGG：Cr^3＋ I：Theory

A theory for shifts of energy spectra due to electron-phonon interaction (EPI) has been developed. Both the temperature-independent contributions and the temperature-dependent ones of acoustic branches and optical branches have been derived. It is found that the temperature-independent contributions are very important, especially at low temperature. The total pressure-induced shift (PS) of a level (or spectral line or band) is the algebraic sum of its PS without EPI and its PS due to EPI. By means of both the theory for shifts of energy spectra due to EPI and the theory for PS of energy spectra, the total PS of R1 line of tunable laser crystal GSGG:Cr3+ at 70 K as well as the ones of its R1 line, R2 line and U band at 300 K will be successfully calculated and explained in this series of papers.

Immunogenicity and tumor-inhibiting effects of HSP-antigen peptide complex in melanoma B16 cells in mice

目的 :黑色素瘤 B1 6细胞热休克蛋白 -抗原肽复合物 (HACs)及其粗提物 (HAC- CEs)的制备 ,以及它们的免疫原性和抑瘤效应的研究。方法 :应用 Sephacryl S- 2 0 0凝胶过滤制备HAC- CEs,应用亲和层析纯化 HACs,并测其免疫功能和抑瘤效应。结果 :应用凝胶过滤制备的HAC- CE3、HAC- CE4、HAC- CE5和应用亲和层析纯化的 HAC60 ,HAC75和 HAC97均不同程度地降低肿瘤发生率、延迟肿瘤发生时间和减少移植黑色素瘤 C57BL/6J小鼠死亡率 ;同时 ,伴有小鼠脾细胞 IFN-γ和 IL- 2分泌活性及 CTL杀伤率的增加。结论 :分子量为 60 0 0 0～ 970 0 0的 HACs具有免疫原性和抑瘤效应 ,本研究为制备肿瘤疫苗提供重要的实验依据。

补肾调经汤对PCOS模型大鼠卵巢t-PA、PAI-1表达的影响

目的:观察补肾调经汤对PCOS模型大鼠卵巢t-PA、PAI-1表达的影响。方法:用来曲唑造模法建立雌性大鼠PCOS模型,设置正常对照组、模型组、西药组、中药组。通过阴道涂片观察大鼠动情周期,ELISA法检测大鼠血清T水平,电镜观察卵巢形态,Western blot检测测定大鼠卵巢t-PA、PAI-1蛋白的表达。结果:与正常对照组比较,模型组大鼠血清T水平升高,窦卵泡数增多,颗粒细胞层数减少,卵巢组织t-PA表达降低、PAI-1表达升高(P<0.01);与模型组比较,中药组和西药组血清T水平下降,窦卵泡数减少,颗粒细胞层数增多,卵巢组织t-PA表达升高、PAI-1表达降低(P<0.01)。结论:PCOS的发病可能与卵巢t-PA、PAI-1的异常表达有关,补肾调经汤可能能够通过调节t-PA、PAI-1的表达恢复卵巢正常生殖功能,维持生殖内分泌稳态,达到治疗PCOS的作用。

Evaluation of Acute Toxicity and Sedative Effects of Ethylic Extract of <i>Thymelaea lythroides</i>in Wistar Rats

The purpose of this study was to evaluate the acute toxicity and sedative effect of the ethylic extract of Thymelaea lythroides (Tl) on Wistar rats. The study was based on the gavage administration of the ethylic extract of Tl with different doses (i.e. 200, 400, 500 and 5000 mg/kg), where the toxicity and the sedative activity of the plant were observed and the obtained results were analyzed using the ANOVA test followed by the Bonferroni test. The main results of this study showed that the lethal dose of the ethylic extract of Tl was higher than the 5000 mg/kg dose. Moreover, when compared to the control rats, the animals treated with 5000 mg/kg of the extract exhibited significant reduction in appetite for food and water in the first week. Furthermore, results of the psychotropic test conducted during this study showed visible effects on the exploratory activity of the rats. The dose of 5000 mg/kg was also found to have a significant central nervous system effect;the locomotor activities of the animals decreased in a manner similar to those administered with Zepam as control.

Effects of Propolis on Peripheral White Cells, Antioxidative Activity and Tumor Growth in Irradiated mice

The effect of continuous water-soluble propolis administration on radioactivity-induced reduction of hemocytes, and the antioxidant and antitumor effects were investigated. Following a 1-week adjustment period, water-soluble propolis was administered intraperitoneally to male ICR mice at a dose of 100 mg/kg every other day for 2 weeks. Following administration, 2 Gy whole-body irradiation was performed and the counts of leukocytes, lymphocytes, and granulocytes and monocytes in the peripheral blood were determined 1, 3, 7, 15 and 30 days after irradiation. In the second experiment, water-soluble propolis was similarly administered to the mice for 2 weeks after a 1-week adjustment period, and 2 Gy whole-body irradiation was performed. The antioxidant effects in hemocytes were then investigated using 2,2＇-azobis（2-amidinopropane） dihydrochloride （AAPH）, a radical generator. In the third experiment, 1×10^6 Sarcoma-180 cells were inoculated into the right thigh of mice, which were divided into four groups： control, water-soluble propolis-treated, 6 Gy irradiated and water-soluble propolis-treated ＋ 6 Gy irradiated groups, and changes in tumor size were measured for 20 days. Results show that administration of water-soluble propolis inhibits the reduction of hemocytes caused by whole-bodv irradiation, enhances antioxidant effects against radioactivity, and inhibits tumor growth.

COS Activation by Zr^+ in the Gas Phase:A Case of Two-state Reactivity Process

To elucidate the mechanisms of Zr ＋ reacting with COS,both the quartet and doublet potential energy surfaces （PESs） for reactions of Zr ＋ （4 F,2 D） with COS in the gas phase have been investigated in detail by means of density functional method （B3LYP）.To obtain more accurate results,the coupled cluster single-point calculations （CCSD（T）） using B3LYP optimized geometries were performed.For the C-O bond activation,the calculated results indicate that both the quartet and doublet states proceed via an insertion-elimination mechanism.For the C-S bond activation,the quartet reaction has an insertion-elimination mechanism,but the doublet reaction is a direct abstraction of the sulfur atom by Zr ＋.The C-S bond activation is found to be energetically more favorable than the C-O bond activation.It is found that the reaction of the 4 F gound state of Zr ＋ to yield ZrO ＋ is spin-forbidden （Zr ＋ （4 F） ＋ COS （1 Σ） → ZrO ＋ （2） ＋ CS （1 Σ）） and the crossing points were approximately determined.All the results have been compared with the existing experimental and theoretical data.

The Comparison of the Control Effect on the Varroa Mite with Different Ways of Raising Management

[Objective] The aim was to o research the control effect to Varroa destructor and Tropilaeplaps clareae whit new raising management methods, and reduce the pollution caused by drug treatment of bee. [ Metkod] We took the method that was replace the hive and comb on test col- onies with the comb Formalin soaking liquid used three days after the dry and sterilization in advance with the hive. The test groups were 10 colonies randomly selected from the disinfected comb in the breeding. This was the first kind of method. The control groups were 10 colonies randomly se- lected by the conventional breeding management methods. From January 20th, statistic analysis on parasitic situation of bees once every month be- tween the two methods, and take corresponding measures to prevent and control the varroa mite according to the varroa mite parasitic number in time, then the control effect of mite between the two methods were compared with. [ Result] The results showed that the first method was better than the second one in V. destructor, the control time of T. clareae delayed about one month [ Conclusion] Good breeding method could restrain I V. destructor and T. clareae parasitism, speeded up the colony development, and reduced the pollution of chemical drugs on bee products, regar- dod as a kind good method of controlling bee mites to make use of the feeding and management technology.

奥希替尼在老年非小细胞肺癌患者靶向治疗中的应用效果及对T细胞水平的影响

目的 探讨奥西替尼在老年非小细胞肺癌患者靶向治疗中的效果及对免疫水平的影响。方法 回顾性选择2018年1月至2020年12月老年非小细胞肺癌患者116例研究,根据治疗方法不同分为2组,各58例。对照组采用常规放化疗治疗,观察组在对照组基础上联合奥西替尼治疗,3个月治疗后评估患者效果,比较2组总有效率、T细胞水平(CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))、肿瘤标志物水平、不良反应发生率。结果 观察组治疗3个月总有效率为44.8%高于对照组25.9%(P<0.05);2组治疗后3个月CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)水平均低于治疗前(P<0.05);CD8^(+)水平高于治疗前(P<0.05);观察组治疗后3个月CD3^(+)(58.95±4.21)%、CD4^(+)(32.59±3.11)%、CD4^(+)/CD8^(+)(1.21±0.22)高于对照组(P<0.05);CD8^(+)(26.81±3.32)%低于对照组(P<0.05);观察组干预3个月后CA125(91±8)U/ml、CYFRA21-1(1.26±0.24)μg/L及癌胚抗原(CEA)水平(34±5)μg/L均低于对照组(P<0.05);2组不良反应发生率差异无统计学意义(P>0.05)。结论 奥西替尼用于老年非小细胞肺癌患者靶向治疗中,能获得较好的总有效率,对患者T细胞水平影响较小,可降低肿瘤标志物水平,未增加不良反应发生率,值得临床推广应用。

Pressure-Induced Shift of R-Line of MgO：Cr^3＋ with Electron-Phonon Interaction Effect

By means of improved ligand-field theory, the ＂pure electronic＂ pressure-induced shift （PS） and the PS due to electron-phonon interaction （EPI） of R-line of MgO：Cr^3＋ have been calculated, respectively. The calculated results are in very good agreement with the experimental data. The behaviors of the pure electronic PS of R-line of MgO：Cr^3＋ and the PS of its R-line due to EPI are different. It is the combined effect of them that gives rise to the total PS of R-line, which has satisfactorily explained the experimental results. The comparison between the feature of R-line PS of MgO：Cr^3＋ and that of R1-line PS of ruby has been made.