Mesh词表词汇实用例句:“培养技术-Culture Techniques”

例句:The experiments proved upside cells all sensitive to the Dengue virues and the Aedes albopictus C6/36cell is the best.With the development of

Conference on deliberating the high-yielding cultural technique of rice

The new high-yielding cultural technique of rice was constituted by the high percentage of ear-bearing til-ler,high filled-grain number,high economic index and stable high-yielding panicle number,abbreviatedas"Three Highs and One Stable"(THOS).It has been deliberated and summarized by Zhejiang Academy

High-frequency magnetic stimulation attenuates beta-amyloid protein 1-42 neurotoxicity in organotypic hippocampal slices

Repetitive transcranial magnetic stimulation （rTMS） has been utilized as a therapeutic tool for neurodegenerative disorders including Alzheimer＇s disease. However, the precise mechanisms of its clinical effects remain unknown. β-amyloid （Aβ） exhibits direct neurotoxic effects and is closely related to neuronal degeneration in Alzheimer＇s disease. Therefore, it has been hypothesized that the neuroprotective effects of rTMS are related to the mechanisms of protection against Aβ neurotoxicity. Organotypic hippocampal slices were prepared from 8-day old, Sprague Dawley rats. The tissue slices were exposed to 100 μmol/L Al3142 since day 12 in vitro with and without high-frequency （20 Hz） magnetic stimulation. Magnetic stimulation efficacy was evaluated by measuring neuronal nuclei （NeuN） protein expression and by observing cultures following propidium iodide fluorescence staining and bromodeoxyuridine （BrdU） immunohistochemistry. Lactate dehydrogenase activity was detected in the culture media to evaluate hippocampal neuronal damage. Our results demonstrated that high-frequency magnetic stimulation significantly reversed the reduction of NeuN protein expression because of Aβ1-42 exposure （P 〈 0.05） and significantly reduced the number of damaged cells in the hippocampal slices （P 〈 0.05）. However, lactate dehydrogenase levels and anti-BrdU staining results did not reveal any statistical differences These findings indicate that high-frequency magnetic stimulation might have protective effect on hippocampal neurons from Aβ1-42 neurotoxicity.

Thermal Diversities of Two Na^+/H^+ Exchanges in Guinea Pig Red Cells

Objective\ To test the effect of hypothermia on Na+/H+ exchange, activated by shrinkage and cytoplasmic acidosis. Method\ Amiloride-sensitive Na+ influx in guinea pig red cells was traced with isotope 22Na and intracellular Na+ concentration was measured by emission flame photometry. Result\ Amiloride-sensitive Na+ influx decreased linearly as a function of temperatures (about 37℃) in shrunken cells, but increased in acidified cells. The up-regulation of acid-induced Na+/H+ exchange by elevated temperature was enhanced by hypo-osmolarity. Less sensitivity of intracellular H+ site at 41℃ may be the mechanism for the inhibition of shrinkage-induced Na+/H+ exchange by elevated temperature. Heating-mediated explosive increase in the activity of acid-induced Na+/H+ exchange may be due to enhanced extracellular Na+ sensitivity and lower intracellular pH caused by acidic metabolites. Acid-induced Na+/H+ ewxchange contributes to cytoplasmic Na+ accumulation. Conclusion\ These two modes of Na+/H+ exchange with different response to elevated temperature may play different roles in the cellular pathogenesis of heatstroke.

Optimization of transfection efficiency of small interfering RNA in purified human prolactinoma cells

Background Control of hypersecretion of certain hormones is one of the key targets in the treatment of pituitary adenomas. RNA interference has been shown to inhibit protein expression, and thus it may represent a promising method for the treatment of pituitary adenomas. In the present study, transfection efficiency of small interfering RNA （siRNA） was optimized in human prolactinoma cells. Methods First, a method was optimized to extract highly purified human prolactinoma cells in vitro. The extracted cells were verified to retain the physiological features of prolactin （PRL） secretion. Second, three conditions for siRNA transfection were tested by the evaluation of transfectfon efficiency and cell viability. The proper transfection condition was verified for human prolactinoma cells. Third, the siRNA for prolactin was transfected into the human prolactinoma cells, and the suppression of PRL mRNA was evaluated by quantitative real-time reverse transcription-PCR. Results The siRNA of 100 pmol with Lipofectamine 2000 of 5 μl for 1×10^6 cells was proved preferable, with transfection efficiency being 53.3% and cell viability being 69.7%. In the preliminary experiment the siRNA against PRL decreased the mRNA of PRL by 34.0%. Conclusion It is possible to inhibit hormone hypersecretion by RNA interference, that may eventually enable therapeutic siRNA drugs developed.

细胞因子诱导的杀伤细胞体外培养的初步研究(英文)

Objective To investigate the culture method of cytokines induced killer cells using CD3 monoclonal antibody,IL-2,IFN-γ and IL-1α in vitro.Methods Peripheral blood mononuclear cells (PBMC) were collected by a blood cell separator from 8 patients with refractory lymphoma,then expanded by priming them with recombinant IFN-γ,monoclonal antibody (mAb) to CD3 and human recombinant IL-1α,followed by adding recombinant IL-2 the next day.The CIK cells were counted on d1,4,7,10,13 of incubation,respectively.The phenotypic patterns were characterized before culture and on d13 of culture.Results CIK cells colony formed on d3.On d13,the CIK cells counting reached up to (7-18)×109(mean 12.7×109),multiplying 44-140 times(mean 98 times).Cell survival rate was over 90%.The average percentage of cells expressing CD3+,CD4+,CD8+ and CD3+ CD56+ were also increased from (50.9±3.5)%,(29.9±1.7)%,(41.3±3.2)%,(1.6±0.2)% to (90.2±1.6)%,(40.6±5.5)%,(52.8±4.9)% and (33.1±4.0)%,respectively.Conclusions CIK cells developed by the culture method have high in vitro proliferate rate and tumor-killing capacity.The simplicity of the operation and autogenic source of the cells indicate the method could be applied clinically treating patients with refractory lymphoma.

Application of Arbuscular Mycorrhizal Inocula Might Be A Promising Method in the Restoration of Severely Degraded Wetlands

The application of arbuscular mycorrhizal (AM) inocula in severely degraded wetlands could ensure success in restoration.Mycorrhizal fungi play an important role in plant individual's survival and development in a low nutrient condition.Based on the importance that mycorrhizal fungi have to their host plants,mycorrhizal inocula have been produced and applied in terrestrial ecosystems in order to let the plants become mycorrhizal.However,mycorrhizal inocula have not been used in wetland restorations,despite increasing evidence that mycorrhizal fungi are commonly found in various wetland systems and have the ability to survive under anoxic conditions.Evidence also shows that mycorrhizal fungal inocula in the soil could have been destroyed in the degraded wetland or could be destroyed during traditional wetland restoration process.Therefore,AM inocula production is strongly recommended for wetland restoration.In this paper,I will argue that AM inocula production is required when introduced recovery is necessary,and aeroponic culture technique is a preferable method to produce AM inocula.Last,a renewed wetland restoration flow chart is summarized.

Experimental assessment of regenerative properties of platelet rich plasma on the human skin-a review

Several studies demonstrated the favorable effects of platelet rich plasma(PRP)on the skin and promoted its wide use in clinical practice.The growth factors stored in platelet alfa-granules allow for the tissue regeneration and the main fields of application of PRP in current clinical practice are the cartilage and musculoskeletal defects,osteoarthritis and other bone disorders,chronic and difficult to heal wounds,and aesthetic procedures.The relevant number of different PRP preparation protocols may explain the inconsistency of the different clinical outcomes reported in the literature.Despite the technological advances in PRP preparation,the objective assessment of the clinical efficacy of PRP from the literature reports still is difficult due to the low homogeneity of the samples in terms of both inclusion criteria and size.Therefore,it might be useful to establish standardized and reproducible experimental models to confirm and objectively measure the effectiveness of the available clinical results.Many experimental investigations have been carried out to objectively assess the effectiveness of PRP and platelet gel on several tissues.As far as the skin is concerned,the studies carried out to date are limited to fibroblasts in in-vitro culture models or to collagen,vascular supply,epithelium,and hair follicle in in-vivo models.The skin,however,is a very complex organ,where different cell lines coexist and feature complex mutual interaction.A model that combines the advantages of both in-vitro and in-vivo cultures is the ex-vivo model.The demonstration of the platelet derived growth factors effects through the ex-vivo human full-thickness skin culture model is a keystone to support the evidence of the PRP effectiveness,as it represents an objective,fast,reproducible,and ethical investigational method.