In order to precisely recognize and karyotype Brassica napus L. chromosomes, C0t-1 DNA was extracted from its genomic DNA, labeled with biotin-1 1-dUTP and in situ hybridized. The hybridized locations were detected by...In order to precisely recognize and karyotype Brassica napus L. chromosomes, C0t-1 DNA was extracted from its genomic DNA, labeled with biotin-1 1-dUTP and in situ hybridized. The hybridized locations were detected by Cy3-conjugated streptavidin. Specific fluorescence in situ hybridization （FISH） signal bands were detected on all individual chromosome pairs. Each chromosome pair showed specific banding patterns. The B. napus karyotype has been constructed, for the first time, on the basis of both Cot-1 DNA FISH banding patterns and chromosome morphology.展开更多
Total DNA is isolated from Porphyra cells which have been prepared from sea snail enzyme digests of Porphyra thalli. The crude extracts of total DNA are rapidly purified with Wizard DNA Clean-up resin by using modifie...Total DNA is isolated from Porphyra cells which have been prepared from sea snail enzyme digests of Porphyra thalli. The crude extracts of total DNA are rapidly purified with Wizard DNA Clean-up resin by using modified washing solution. DNA bands are clearly observed in the restriction endonuclease patterns of P. yezoensis and P. haitanensis total DNAs. Both P. yezoensis and P. haitanensis have specific DNA bands in the restriction patterns of EcoRI, PastI and HaeIII. According to the band pattern of total DNA, the discrimination between P. yezoensis and P. haitanensis is very easy especially in the restriction pattern of HaeIII. The comparative results indicate that the band pattern of total DNA is a new type and reliable DNA marker for identification and classification of Porphyra species.展开更多
文摘In order to precisely recognize and karyotype Brassica napus L. chromosomes, C0t-1 DNA was extracted from its genomic DNA, labeled with biotin-1 1-dUTP and in situ hybridized. The hybridized locations were detected by Cy3-conjugated streptavidin. Specific fluorescence in situ hybridization （FISH） signal bands were detected on all individual chromosome pairs. Each chromosome pair showed specific banding patterns. The B. napus karyotype has been constructed, for the first time, on the basis of both Cot-1 DNA FISH banding patterns and chromosome morphology.
文摘Total DNA is isolated from Porphyra cells which have been prepared from sea snail enzyme digests of Porphyra thalli. The crude extracts of total DNA are rapidly purified with Wizard DNA Clean-up resin by using modified washing solution. DNA bands are clearly observed in the restriction endonuclease patterns of P. yezoensis and P. haitanensis total DNAs. Both P. yezoensis and P. haitanensis have specific DNA bands in the restriction patterns of EcoRI, PastI and HaeIII. According to the band pattern of total DNA, the discrimination between P. yezoensis and P. haitanensis is very easy especially in the restriction pattern of HaeIII. The comparative results indicate that the band pattern of total DNA is a new type and reliable DNA marker for identification and classification of Porphyra species.
