Biocompatibility of dental porcelain is of crucial importance to the long-term success of dental prostheses because of its close contact with oral tissues for extended periods. This investigation was aimed to evaluate...Biocompatibility of dental porcelain is of crucial importance to the long-term success of dental prostheses because of its close contact with oral tissues for extended periods. This investigation was aimed to evaluate cellular responses to locally produced dental porcelain （Universiti Sains Malaysia, Malaysia） after a short-term subcutaneous implantation in a rat model. Locally produced dental porcelains supplied in disc form were implanted subcutaneously into 12 Sprague-Dawley male albino rats, which were sacrificed in groups of 3 at 1, 2, 3 and 4 weeks after implantation. A semi-quantitative histological analysis of the tissue surrounding implanted discs was done under an image analyzer after staining with hematoxyline and eosin. The macrophage was clearly the dominant cell type at the implant surface at the first week after implantation, followed by a gradual decrease as the implantation period increased. On the contrary, fibroblasts and fibrocytes were the dominant cell types in the tissue surrounding the implanted discs at the third and fourth week after implantation with the appearance of mature collagen. From pathological point of view, the disappearance of inflammatory cellular responses and the well matured fibrous connective tissue surrounding the implanted discs indicate a satisfactory in vivo biocompatibility.展开更多
A glassceramic material,which can be used in stomatology for production of dentinal layer of the dental crown multilayer coating,was received. In order to colour the material the admixtures of Tb and Ce compounds were...A glassceramic material,which can be used in stomatology for production of dentinal layer of the dental crown multilayer coating,was received. In order to colour the material the admixtures of Tb and Ce compounds were used,as well as composite admixture representing a mixture of Ce,Nd and La oxides. It was demonstrated that the admixture of Tb oxide tones the material only when it is used together with Ce oxide. It was found that the more admixtures of rare earth elements oxides are contained in the sample composition,the higher the colour strength of the sintered material is. The wave length for the test samples is in the range of 600~650 nm,which corresponds to the yellow-orange and orange-red spectral range.展开更多
In order to well design tribosystems of dental CAD-CAM restorations, an understanding of the tribological mechanisms of dental machinable porcelain are essential. The friction and wear behavior of new generation indus...In order to well design tribosystems of dental CAD-CAM restorations, an understanding of the tribological mechanisms of dental machinable porcelain are essential. The friction and wear behavior of new generation industrially prefabricated Cerec Vitablocs Mark II against uniform Si3N4 ball has been performed using a small amplitude reciprocating apparatus under simulating oral conditions. The loads of 10-40 N, reciprocating amplitudes of 100-500 urn, frequencies of 1-4 Hz and two lubrications (no / artificial saliva lubrication) were selected. Tests lasting up to 10 000 cycles were conducted. The results show that Cerec Vitablocs Mark II record a friction coefficient of 0.55-0.84. Artificial saliva plays a lubricant effect during wear process. Among three parameters of the test on friction coefficient and wear depth of dental machinable porcelains, the load effect is prominent. Abrasive wear is the main wear mechanism, but brittle cracks and delamination are more popular especially under unlubricated friction.展开更多
目的:通过对临床损伤失效的全瓷修复体的损伤形貌观察,分析全瓷修复体饰瓷疲劳损伤的机制和过程,以及缺陷与饰瓷损伤的关系。方法:收集断裂或破碎的全瓷修复体,体式显微镜和扫描电子显微镜(scanning electron microscope,SEM)观察其断...目的:通过对临床损伤失效的全瓷修复体的损伤形貌观察,分析全瓷修复体饰瓷疲劳损伤的机制和过程,以及缺陷与饰瓷损伤的关系。方法:收集断裂或破碎的全瓷修复体,体式显微镜和扫描电子显微镜(scanning electron microscope,SEM)观察其断裂面和咬合面微观形貌以及饰瓷的缺陷,X线衍射能谱分析(energy-dispersive X-ray spectroscopy,EDS)饰瓷内不均质部分的化学成分。运用陶瓷损伤形貌分析的方法,分析全瓷修复体的断裂方式和损伤特征。结果:全瓷修复体的最终损伤失效包括完全断裂、饰瓷崩瓷和饰瓷脱瓷3种方式。氧化铝全瓷冠发生了完全断裂,氧化锆全瓷冠发生饰瓷崩瓷或饰瓷脱瓷。饰瓷疲劳损伤包括表面磨损、锥状裂纹和饰瓷脱瓷3种模式。饰瓷表面发现气孔和污染颗粒缺陷。结论:全瓷修复体应设计多点咬合接触,分散咬合应力。饰瓷脱瓷发生于饰瓷层过薄的情况,适当的饰瓷厚度保证饰瓷的抗疲劳损伤能力。临床需严格控制饰瓷烧结过程的环境污染。展开更多
目的:体外评价新型根尖倒充填材料iRoot BP plus和iRoot FS的生物学性能。方法:(1)将牙根预备成长3 mm、根管直径1 mm的试样,分别于根管内填充iRoot BP plus、iRoot FS和三氧化矿物凝聚体(mineral trioxide aggregate,MTA)。将制备好的...目的:体外评价新型根尖倒充填材料iRoot BP plus和iRoot FS的生物学性能。方法:(1)将牙根预备成长3 mm、根管直径1 mm的试样,分别于根管内填充iRoot BP plus、iRoot FS和三氧化矿物凝聚体(mineral trioxide aggregate,MTA)。将制备好的试样置于模拟体液(simulated body fluid,SBF)中,用扫描电子显微镜观察暴露的材料表面矿物沉积情况,并通过X射线能谱对其表面形成的晶体进行元素分析,测定SBF的p H值随时间的变化。(2)将iRoot BP plus、iRoot FS和MTA制备成直径8 mm、高度2 mm的圆柱,制备DMEM浸提液,通过MTT实验观察材料对MG63细胞增殖活性的影响,并通过实时荧光定量PCR和碱性磷酸酶(alkaline phosphatase,ALP)染色分析材料对ALP基因及蛋白表达的影响。结果:(1)在SBF中浸泡24 h时,iRoot BP plus、iRoot FS和MTA表面均已有矿物沉积,14 d后有大量矿物沉积,钙∶磷比值分别为1.43、1.39和1.51;(2)iRoot BP plus、iRoot FS和MTA均可使SBF的p H升高,3周时分别为8.09±0.07、7.91±0.06和8.11±0.06;(3)以体积比为1∶5和1∶10稀释的iRoot BP plus、iRoot FS和MTA浸提液对MG63细胞的增殖无明显促进或抑制作用;(4)iRoot BP plus、iRoot FS和MTA组对MG63细胞ALP基因的表达均具有促进作用,ALP染色各组未见明显差异。结论:iRoot在模拟体液中可诱导矿物质沉积,促进MG63细胞的分化和矿化,具有良好的生物活性和促成骨作用。展开更多
文摘Biocompatibility of dental porcelain is of crucial importance to the long-term success of dental prostheses because of its close contact with oral tissues for extended periods. This investigation was aimed to evaluate cellular responses to locally produced dental porcelain （Universiti Sains Malaysia, Malaysia） after a short-term subcutaneous implantation in a rat model. Locally produced dental porcelains supplied in disc form were implanted subcutaneously into 12 Sprague-Dawley male albino rats, which were sacrificed in groups of 3 at 1, 2, 3 and 4 weeks after implantation. A semi-quantitative histological analysis of the tissue surrounding implanted discs was done under an image analyzer after staining with hematoxyline and eosin. The macrophage was clearly the dominant cell type at the implant surface at the first week after implantation, followed by a gradual decrease as the implantation period increased. On the contrary, fibroblasts and fibrocytes were the dominant cell types in the tissue surrounding the implanted discs at the third and fourth week after implantation with the appearance of mature collagen. From pathological point of view, the disappearance of inflammatory cellular responses and the well matured fibrous connective tissue surrounding the implanted discs indicate a satisfactory in vivo biocompatibility.
文摘A glassceramic material,which can be used in stomatology for production of dentinal layer of the dental crown multilayer coating,was received. In order to colour the material the admixtures of Tb and Ce compounds were used,as well as composite admixture representing a mixture of Ce,Nd and La oxides. It was demonstrated that the admixture of Tb oxide tones the material only when it is used together with Ce oxide. It was found that the more admixtures of rare earth elements oxides are contained in the sample composition,the higher the colour strength of the sintered material is. The wave length for the test samples is in the range of 600~650 nm,which corresponds to the yellow-orange and orange-red spectral range.
基金supported by grant 50305030 from the Chinese Natural Science Foundationgrant 2003033526 from China Postdoctoral Science Foundationgrant 03SG022 from Sicence an d Technology Office of Sichuan Province.
文摘In order to well design tribosystems of dental CAD-CAM restorations, an understanding of the tribological mechanisms of dental machinable porcelain are essential. The friction and wear behavior of new generation industrially prefabricated Cerec Vitablocs Mark II against uniform Si3N4 ball has been performed using a small amplitude reciprocating apparatus under simulating oral conditions. The loads of 10-40 N, reciprocating amplitudes of 100-500 urn, frequencies of 1-4 Hz and two lubrications (no / artificial saliva lubrication) were selected. Tests lasting up to 10 000 cycles were conducted. The results show that Cerec Vitablocs Mark II record a friction coefficient of 0.55-0.84. Artificial saliva plays a lubricant effect during wear process. Among three parameters of the test on friction coefficient and wear depth of dental machinable porcelains, the load effect is prominent. Abrasive wear is the main wear mechanism, but brittle cracks and delamination are more popular especially under unlubricated friction.
文摘目的:通过对临床损伤失效的全瓷修复体的损伤形貌观察,分析全瓷修复体饰瓷疲劳损伤的机制和过程,以及缺陷与饰瓷损伤的关系。方法:收集断裂或破碎的全瓷修复体,体式显微镜和扫描电子显微镜(scanning electron microscope,SEM)观察其断裂面和咬合面微观形貌以及饰瓷的缺陷,X线衍射能谱分析(energy-dispersive X-ray spectroscopy,EDS)饰瓷内不均质部分的化学成分。运用陶瓷损伤形貌分析的方法,分析全瓷修复体的断裂方式和损伤特征。结果:全瓷修复体的最终损伤失效包括完全断裂、饰瓷崩瓷和饰瓷脱瓷3种方式。氧化铝全瓷冠发生了完全断裂,氧化锆全瓷冠发生饰瓷崩瓷或饰瓷脱瓷。饰瓷疲劳损伤包括表面磨损、锥状裂纹和饰瓷脱瓷3种模式。饰瓷表面发现气孔和污染颗粒缺陷。结论:全瓷修复体应设计多点咬合接触,分散咬合应力。饰瓷脱瓷发生于饰瓷层过薄的情况,适当的饰瓷厚度保证饰瓷的抗疲劳损伤能力。临床需严格控制饰瓷烧结过程的环境污染。
文摘目的:体外评价新型根尖倒充填材料iRoot BP plus和iRoot FS的生物学性能。方法:(1)将牙根预备成长3 mm、根管直径1 mm的试样,分别于根管内填充iRoot BP plus、iRoot FS和三氧化矿物凝聚体(mineral trioxide aggregate,MTA)。将制备好的试样置于模拟体液(simulated body fluid,SBF)中,用扫描电子显微镜观察暴露的材料表面矿物沉积情况,并通过X射线能谱对其表面形成的晶体进行元素分析,测定SBF的p H值随时间的变化。(2)将iRoot BP plus、iRoot FS和MTA制备成直径8 mm、高度2 mm的圆柱,制备DMEM浸提液,通过MTT实验观察材料对MG63细胞增殖活性的影响,并通过实时荧光定量PCR和碱性磷酸酶(alkaline phosphatase,ALP)染色分析材料对ALP基因及蛋白表达的影响。结果:(1)在SBF中浸泡24 h时,iRoot BP plus、iRoot FS和MTA表面均已有矿物沉积,14 d后有大量矿物沉积,钙∶磷比值分别为1.43、1.39和1.51;(2)iRoot BP plus、iRoot FS和MTA均可使SBF的p H升高,3周时分别为8.09±0.07、7.91±0.06和8.11±0.06;(3)以体积比为1∶5和1∶10稀释的iRoot BP plus、iRoot FS和MTA浸提液对MG63细胞的增殖无明显促进或抑制作用;(4)iRoot BP plus、iRoot FS和MTA组对MG63细胞ALP基因的表达均具有促进作用,ALP染色各组未见明显差异。结论:iRoot在模拟体液中可诱导矿物质沉积,促进MG63细胞的分化和矿化,具有良好的生物活性和促成骨作用。
