Influence of Moxibustion Serum on the Expression of Fas bcl-2 mRNA and Protein of EL-4 Lymphoma Cells

Objective： To observe the influence of moxibustion serum of mice on the expression of Fas, bcl-2 mRNA and protein of EL-4 lymphoma cells in vitro. Method： The EL-4 lymphoma cells were cultivated for 24 h in the moxibustion serum of mice. The expression of Fas and bcl-2 mRNA of EL-4 lymphoma cells were detected by in-situ hybridization method, and the protein expression of Fas and bcl-2 were observed by the immuocytochemistry method. Results： The expression of bcl-2 mRNA and protein decreased , and the expression of Fas mRNA and protein increased significantly in EL-4 cells, which were cultivated in the moxibustion serum compared those cultivated in normal mice serum （P〈0.05）. Conclusion： Moxibustion serum could down-regulate the bcl-2 mRNA and protein and up-regulate the Fas mRNA and protein of EL-4 cells.

Effect of oxymatrine on murine fulminant hepatitis and hepatocyte apoptosis

To evaluate the protective effects and mechanism of action of oxymatrine (OM) on the experimental fulminant hepatitis (FH) and early hepatocyte apoptosis in mur ine liver tissue Methods Fulminant hepatitis mice were induced by injecting lipopolysaccharide (LPS) intr aperitoneally (ip) in galactosamine (GalN) sensitized mice Two separate experim ents were designed, including saline control group, fulminant hepatitis group an d oxymatrine pretreated group (50?mg/kg, intraperitoneally, bid×3 days) The leve ls of serum tumor necrosis factor alpha (TNFa) in mice from two experiments were determined at 5 hour and 7 5 hour after injecting galactosamine/lipopolysacc haride Mouse liver samples at 5 hour time point were obtained for in situ end labeling (ISEL) staining and ultrastructural observation of apoptotic cells und er transmission electron microscope (TEM) Liver samples at 7 5 hour time poi nt were taken for hematoxylin eosin (HE) staining and immunohistochemical stain ing of Fas and its ligand (FasL) Results As compared with the fulminant hepatitis group, the levels of serum tumor necros is factor alpha in mice from the OM pretreated group at 5 hour and 7 5 hour t ime point were all significantly decreased ( P 【0 05 and P 【0 01 respecti vely) Hepatocyte apoptosis in mice at 5 hour time point was significantly inh ibited ( P 【0 01) Both the degree of liver injury and the degree of Fas and Fas ligand expression in the OM pretreated group were reduced remarkably ( P 【0 01 and 0 05 respectively) when compared with the saline control group Conclusions Oxymatrine protects mice from fulminant hepatitis induced by GalN/LPS and may bl ock hepatocyte apoptosis and subsequent necrosis through downregulating the prod uction of serum tumor necrosis factor alpha and the expression of Fas and Fas li gand in liver tissue