Fusarium monilljorme, a fungus of established carcinogenic potential, is one of the most common fungal contaminants of maize, millet and other grains in Linxian County, China. Fusarin C, a major product of F. monilljo...Fusarium monilljorme, a fungus of established carcinogenic potential, is one of the most common fungal contaminants of maize, millet and other grains in Linxian County, China. Fusarin C, a major product of F. monilljorme grown on corn in the laboratory, is mutagenic in Salmonella tester strains and in V79 cells. Fusarin C showed several characteristics of malignant transformation including the implantation of the rat esophageal epithelial cell line (RE ?525) in nude mice. The present work demonstrated that fusarin C can induce esophageal and forestomach carcinomas in DBA mice and Wistar rats, and thus the experimental results substantiated further the carcinogenicity of fusarin C.展开更多
Fusarium moniliforme Sheld.is a rice pathogenic fungus and causes the disease called Bakanae,which has increasingly damaged rice production in the recent years. Trichoderma spp. has been one of the most widely used bi...Fusarium moniliforme Sheld.is a rice pathogenic fungus and causes the disease called Bakanae,which has increasingly damaged rice production in the recent years. Trichoderma spp. has been one of the most widely used biological control agent of plant disease. By geneticaly labelling F. moniliforme with the GFP reporter gene, we have studied the antagonistic action of Trichoderma viride against this pathogenic fungus. The binary GFP reporter vector pCHF3-35S∷GFP was constructed, which carries the gfp gene driven by the CaMv35S promoter. The vector was transformed into F. moniliforme via Agrobacterium.The mycoparasitism of T.viride against F.moniliforme was tested by dual culture and examined with fluorescence microscope. The result of the dual culture showed that the T.viride maintained a strong competitive ability against F. moniliforme , by growing on the top of the pathogen colony. Fluorescence microscope observation indicated that attacked hyphae of F. moniliform were distorted, swollen or broken. This indicate an enzymatic by T.viride to degrade the host cell walls and used the cell contents as a source of nutrients (Fig 1) .展开更多
For studying on pathogenicity mechanism of Fusarium moniliforme,REMI was used to transform protoplasts of FT1 strain with the vector pUCATPH,which contained hygromycin B-resistant gene.More than 300 transformants had ...For studying on pathogenicity mechanism of Fusarium moniliforme,REMI was used to transform protoplasts of FT1 strain with the vector pUCATPH,which contained hygromycin B-resistant gene.More than 300 transformants had been obtained,most of them were quite stable after five rounds of successive culture.25 mutants of morphology and 2 weak pathogenicity mutants were gained by REMI.PCR amplification showed that the hygromycin B-resistant gene had integrated into genomes of the two pathogenicity mutants.The optimum conditions of preparing protoplasts were: the mycelia growing in PDB medium for 14 h,lywallzyme was used to digest the mycelia at 100 r/min,30℃ for 4 h,and 0.7 mol/L NaCl was used as the osmotic stabilizer.展开更多
从水稻根际土壤中筛选出拮抗水稻恶苗病的菌株,初步研究其抑菌作用及生防效果。采用平板稀释法从水稻根际土壤中分离获得菌株,以水稻恶苗病菌为靶标菌采用平板对峙法筛选出拮抗菌;通过形态学特征、生理生化特征及16S r DNA序列分析对筛...从水稻根际土壤中筛选出拮抗水稻恶苗病的菌株,初步研究其抑菌作用及生防效果。采用平板稀释法从水稻根际土壤中分离获得菌株,以水稻恶苗病菌为靶标菌采用平板对峙法筛选出拮抗菌;通过形态学特征、生理生化特征及16S r DNA序列分析对筛选出的拮抗菌进行鉴定;检测拮抗菌无菌发酵液对水稻恶苗病菌菌丝生长的影响,同时测定拮抗菌的抑菌谱及进行盆栽实验。分离得到6株拮抗菌,其中有一株对水稻恶苗病菌拮抗作用较强的菌株SH15,经鉴定菌株SH15为多粘类芽孢杆菌。菌株无菌发酵液对水稻恶苗病菌菌丝生长有显著抑制作用;菌株SH15抑菌谱广,对水稻恶苗病菌、层出镰孢菌、棉花枯萎病菌、辣椒疫病菌、棉花黄萎病、黄瓜黑斑病菌均有一定的抑菌活性。水稻盆栽实验表明,接种多粘类芽孢杆菌SH15可显著降水稻恶苗病的发病指数,平均防效高达65.68%。因此,多粘类芽孢杆菌SH15在水稻恶苗病的生物防治方面具有一定的应用价值。展开更多
文摘Fusarium monilljorme, a fungus of established carcinogenic potential, is one of the most common fungal contaminants of maize, millet and other grains in Linxian County, China. Fusarin C, a major product of F. monilljorme grown on corn in the laboratory, is mutagenic in Salmonella tester strains and in V79 cells. Fusarin C showed several characteristics of malignant transformation including the implantation of the rat esophageal epithelial cell line (RE ?525) in nude mice. The present work demonstrated that fusarin C can induce esophageal and forestomach carcinomas in DBA mice and Wistar rats, and thus the experimental results substantiated further the carcinogenicity of fusarin C.
文摘Fusarium moniliforme Sheld.is a rice pathogenic fungus and causes the disease called Bakanae,which has increasingly damaged rice production in the recent years. Trichoderma spp. has been one of the most widely used biological control agent of plant disease. By geneticaly labelling F. moniliforme with the GFP reporter gene, we have studied the antagonistic action of Trichoderma viride against this pathogenic fungus. The binary GFP reporter vector pCHF3-35S∷GFP was constructed, which carries the gfp gene driven by the CaMv35S promoter. The vector was transformed into F. moniliforme via Agrobacterium.The mycoparasitism of T.viride against F.moniliforme was tested by dual culture and examined with fluorescence microscope. The result of the dual culture showed that the T.viride maintained a strong competitive ability against F. moniliforme , by growing on the top of the pathogen colony. Fluorescence microscope observation indicated that attacked hyphae of F. moniliform were distorted, swollen or broken. This indicate an enzymatic by T.viride to degrade the host cell walls and used the cell contents as a source of nutrients (Fig 1) .
文摘For studying on pathogenicity mechanism of Fusarium moniliforme,REMI was used to transform protoplasts of FT1 strain with the vector pUCATPH,which contained hygromycin B-resistant gene.More than 300 transformants had been obtained,most of them were quite stable after five rounds of successive culture.25 mutants of morphology and 2 weak pathogenicity mutants were gained by REMI.PCR amplification showed that the hygromycin B-resistant gene had integrated into genomes of the two pathogenicity mutants.The optimum conditions of preparing protoplasts were: the mycelia growing in PDB medium for 14 h,lywallzyme was used to digest the mycelia at 100 r/min,30℃ for 4 h,and 0.7 mol/L NaCl was used as the osmotic stabilizer.
文摘从水稻根际土壤中筛选出拮抗水稻恶苗病的菌株,初步研究其抑菌作用及生防效果。采用平板稀释法从水稻根际土壤中分离获得菌株,以水稻恶苗病菌为靶标菌采用平板对峙法筛选出拮抗菌;通过形态学特征、生理生化特征及16S r DNA序列分析对筛选出的拮抗菌进行鉴定;检测拮抗菌无菌发酵液对水稻恶苗病菌菌丝生长的影响,同时测定拮抗菌的抑菌谱及进行盆栽实验。分离得到6株拮抗菌,其中有一株对水稻恶苗病菌拮抗作用较强的菌株SH15,经鉴定菌株SH15为多粘类芽孢杆菌。菌株无菌发酵液对水稻恶苗病菌菌丝生长有显著抑制作用;菌株SH15抑菌谱广,对水稻恶苗病菌、层出镰孢菌、棉花枯萎病菌、辣椒疫病菌、棉花黄萎病、黄瓜黑斑病菌均有一定的抑菌活性。水稻盆栽实验表明,接种多粘类芽孢杆菌SH15可显著降水稻恶苗病的发病指数,平均防效高达65.68%。因此,多粘类芽孢杆菌SH15在水稻恶苗病的生物防治方面具有一定的应用价值。
