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Reactions of Oil Palm (Elaeis guineensis Jacq) Progenies to Fusarium Wilt Disease Caused by Fusarium oxysporum f.sp. Elaeidis under Natural Infection
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作者 Oben Tom Tabi Ndam Lawrence Monah Egbe Andrew Enow 《American Journal of Plant Sciences》 CAS 2024年第10期968-987,共20页
The oil palm (Elaeisguineensis Jacq) is used worldwide in commercial agriculture for the production of palm oil, palm kernel oil and palm wine. It produces more oil per plant than any other oil-producing crop in the w... The oil palm (Elaeisguineensis Jacq) is used worldwide in commercial agriculture for the production of palm oil, palm kernel oil and palm wine. It produces more oil per plant than any other oil-producing crop in the world. Production is constrained by several factors among which pests/diseases are of utmost importance. Vascular wilt (VW) caused by Fusarium oxysporum is the most devastating disease infecting this crop. Its soil-borne ecology has made the use of fungicides to manage this disease too expensive and inpragmatic. There is need for concerted research in the breeding and selection of wilt-tolerant progenies as an essential step in the management of Fusarium wilt disease. The study aims to assess the incidence and severity of vascular wilt among tested oil palm progenies, to evaluate the reduction in yield caused by the disease in the susceptible progenies and to identify the wilt-tolerant, high-yielding progenies. The study was carried out at Pamol Plantations Limited (PPL) in Ndian Estate (Ndian Division), in the Southwest Region of Cameroon. Three field trials were evaluated for tolerance/susceptibility to Fusarium wilt. Each trial consisted of 15 oil palm progenies replicated 4 times. Each progeny had 25 oil palm stands in each replicate. Hence, a total of 1500 oil palm stands were assessed. The experimental design was a randomized complete block (RCB) with trial codes: Trial 2001/1, planted in 2001;Trial 2001/2, planted in 2002;Trial 2001/3, planted in 2003. Each trail had an area of 12 ha, with a plant density of 143 palms·ha−1. Wilt incidence, severity, index, and yield were evaluated on 45 progenies from the 3 trails after identifying Fusarium oxysporum from oil palm plant part. Data was subjected to analysis of variance, Fischer’s least significant difference test (LSD) for mean separation. Identification of Fusarium was based on descriptive analysis. Incidence of VW in the 3 trials ranged from 1% - 39%. Also, 45% of infected plants were from progeny 676 while 1% was from progenies 689, 693, 694 and 710. Disease severity was from 0.9 in progeny 686 to 4.55 in 676. Wilt index ranged from 131 for progeny 694 and 710 to 495 for progenies 705. Out of the 45 progenies evaluated, 27 were tolerant (1 < 100) and 18 susceptible (1 ≥ 100). Within the tolerant progenies, 4 were significant (1 < 20) while 5 out of 18 were significantly susceptible (1 ≥ 185). Mean yield reduction of the susceptible progenies was 34.8% while in the tolerant progenies, it increased by 9.5% when compared to their controls. Progenies 702, 703 and 709 are recommended for planting based on the level of tolerance to Fusarium wilt disease and yield. 展开更多
关键词 PROGENIES TOLERANT SUSCEPTIBLE Vascular Wilt fusarium oxysporum Oil Palm
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Biochar alleviates apple replant disease by reducing the growth of Fusarium oxysporum and regulating microbial communities
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作者 Yinghao Liu Can Wang +5 位作者 Ran Chen Weitao Jiang Yun Li Chengmiao Yin Yanfang Wang Zhiquan Mao 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第3期657-671,共15页
Apple replant disease(ARD)negatively affects plant growth and reduces yields in replanted orchards.In this study,biochar was applied to apple replant soil with Fusarium oxysporum.Our aim was to investigate whether bio... Apple replant disease(ARD)negatively affects plant growth and reduces yields in replanted orchards.In this study,biochar was applied to apple replant soil with Fusarium oxysporum.Our aim was to investigate whether biochar could promote plant growth and alleviate apple replant disease by reducing the growth of harmful soil microorganisms,changing soil microbial community structure and improving the soil environment.This experiment included five treatments:apple replant soil(CK),methyl bromide fumigation apple replant soil(FM),replant soil with biochar addition(2%),replant soil with F.oxysporum spore solution(8×10^(7)spores·mL^(-1)),and replant soil with biochar and F.oxysporum spore solution addition.Seedling biomass,the activity of antioxidant enzymes in the leaves and roots,and soil environmental variables were measured.Microbial community composition and community structure were analyzed using 16SrDNA and ITS2 gene sequencing.Biochar significantly reduced the abundance of F.oxysporum and increased soil microbial diversity and richness.Biochar also increased the soil enzyme activities(urease,invertase,neutral phosphatase,and catalase),the biomass(plant height,fresh weight,dry weight)and the activity of antioxidant enzymes(superoxide dismutase,peroxidase,and catalase).The root indexes of apple seedlings was also increased in replant soil by biochar.In sum,biochar promoted the growth of plants,improved the replant soil environment,and alleviated apple replant disease. 展开更多
关键词 BIOCHAR fusarium oxysporum Apple replant disease Soil environment
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WRKY11 up-regulated dirigent expression to enhance lignin/lignans accumulation in Lilium regale Wilson during response to Fusarium wilt 被引量:1
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作者 Jie Deng Zi’e Wang +2 位作者 Wenyun Li Xiaohua Chen Diqiu Liu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第8期2703-2722,共20页
Lilium are highly economically valuable ornamental plants that are susceptible to Fusarium wilt caused by Fusarium oxysporum.Lilium regale Wilson,a wild lily native to China,is highly resistant to F.oxysporum.In this ... Lilium are highly economically valuable ornamental plants that are susceptible to Fusarium wilt caused by Fusarium oxysporum.Lilium regale Wilson,a wild lily native to China,is highly resistant to F.oxysporum.In this study,a WRKY transcription factor,WRKY11,was isolated from L.regale,and its function during the interaction between L.regale and F.oxysporum was characterized.The ectopic expression of LrWRKY11 in tobacco increased the resistance to F oxysporum,moreover,the transcriptome sequencing and UHPLC-MS/MS analysis indicated that the methyl salicylate and methyl jasmonate levels rose in LrWRKY11 transgenic tobacco,meanwhile,the expression of lignin/lignans biosynthesis-related genes including a dirigent(DiR)was up-regulated.The lignin/lignans contents in LrWRKY11-transgenic tobacco also significantly increased compared with the wild-type tobacco.In addition,the resistance of L.regale scales in which LrWRKY11 expression was silenced by RNAi evidently decreased,and additionally,the expression of lignin/lignans biosynthesis-related genes including LrDIR1 was significantly suppressed.Therefore,LrDIR1 and its promoter(PLrDIR1)sequence containing the W-box element were isolated from L.regale.The interaction assay indicated that LrWRKY11 specifically bound to the W-box element in PLrDIR1 and activated LrDIR1 expression.Additionally,β-glucuronidase activity in the transgenic tobacco co-expressing LrWRKY11/PLrDIR1-β-glucuronidase was higher than that in transgenic tobacco expressing PLrDIR1-β-glucuronidase alone.Furthermore,the ectopic expression of LrDIR1 in tobacco enhanced the resistance to F.oxysporum and increased the lignin/lignans accumulation.In brief,this study revealed that LrWRKY11 positively regulated L.regale resistance to F.oxysporum through interaction with salicylic acid/jasmonic acid signaling pathways and modulating LrDIR1 expression to accumulate lignin/lignans. 展开更多
关键词 Lilium regale fusarium oxysporum WRKY transcription factor dirigent
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Antifungal activity against Fusarium oxysporum of quinolizidines isolated from three controlled-growth Genisteae plants:structure-activity relationship implications
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作者 Willy Cely-Veloza Lydia Yamaguchi +2 位作者 Diego Quiroga Massuo J.Kato Ericsson Coy-Barrera 《Natural Products and Bioprospecting》 CSCD 2023年第1期639-649,共11页
The Genisteae tribe belongs to the Fabaceae family.The wide occurrence of secondary metabolites,explicitly high-lighting the quinolizidine alkaloids(QAs),characterizes this tribe.In the present study,twenty QAs(1-20),... The Genisteae tribe belongs to the Fabaceae family.The wide occurrence of secondary metabolites,explicitly high-lighting the quinolizidine alkaloids(QAs),characterizes this tribe.In the present study,twenty QAs(1-20),including lupanine(1-7),sparteine(8-10),lupanine(11),cytisine and tetrahydrocytisine(12-17),and matrine(18-20)-type QAs were extracted and isolated from leaves of three species(i.e.,Lupinus polyphyllus(’rusell’hybrid),Lupinus muta-bilis,and Genista monspessulana)belonging to the Genisteae tribe.These plant sources were propagated under greenhouse conditions.The isolated compounds were elucidated by analyzing their spectroscopical data(MS,NMR).The antifungal effect on the mycelial growth of Fusarium oxysporum(Fox)of each isolated QA was then evaluated through the amended medium assay.The best antifungal activity was found to be for compounds 8(IC_(50)=16.5μM),9(IC_(50)=7.2μM),12(IC_(50)=11.3μM),and 18(IC_(50)=12.3μM).The inhibitory data suggest that some QAs could effi-ciently inhibit Fox mycelium growth depending on particular structural requirements deduced from structure-activity relationship scrutinies.The identified quinolizidine-related moieties can be involved in lead structures to develop further antifungal bioactives against Fox. 展开更多
关键词 FABACEAE Genista LUPINUS fusarium oxysporum QUINOLIZIDINES ANTIFUNGALS
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Production profile and comparison analysis of main toxin components of Fusarium oxysporum f.sp.sesami isolates with different pathogenicity levels
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作者 Hailing Li Yinghui Duan +7 位作者 Guizhen Xu Shuxian Chang Ming Ju Yin Wu Wenen Qu Hengchun Cao Haiyang Zhang Hongmei Miao 《Oil Crop Science》 CSCD 2023年第2期104-110,共7页
Fusarium wilt is a common fungal disease in sesame caused by Fusarium oxysporum f.sp.sesami(FOS).To determine the toxin production profiles of the FOS isolates with different pathogenicity levels under various culture... Fusarium wilt is a common fungal disease in sesame caused by Fusarium oxysporum f.sp.sesami(FOS).To determine the toxin production profiles of the FOS isolates with different pathogenicity levels under various culture conditions,we assessed the content variation of fusaric acid(FA)and 9,10-dehydrofusaric acid(9,10-DFA)produced by the four representative FOS isolates.Results indicated that the concentration of FA reached to a maximum of 2848.66μg/mL in Czapek medium,while 9,10-DFA was mainly produced in Richard and Lowcarbon Richard medium.The concentration of 9,10-DFA on Richard culture medium varied from 0μg/mL to 716.89μg/mL.Of the five culture media used in this study,Czapek culture medium was the most conductive to produce FA.FA production was significantly affected by culture medium,culture time,and their interactions.Results suggest that there is no correlation between toxin production and pathogenicity level of FOS isolates.These findings provide key information for the mechanism analysis of FOS-sesame interaction and pathogen control. 展开更多
关键词 fusarium oxysporum Fusaric acid 9 10-dehydrofusaric acid Pathogenicity level
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5-Aminolevulinic acid against strawberry Fusarium wilt:Bidirectional regulation of biocontrol agents and pathogens
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作者 Hao Yang Jianting Zhang +3 位作者 Haiwen Zhang Rongxiang Cao Donglan Tang Liangju Wang 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第6期1349-1361,共13页
Strawberry Fusarium wilt (SFW) is a systematic soil-borne disease caused by Fusarium oxysporum f.sp.fragaria (Fof),which infects the vascular bundles,blocking water and nutrient transport from roots to the aboveground... Strawberry Fusarium wilt (SFW) is a systematic soil-borne disease caused by Fusarium oxysporum f.sp.fragaria (Fof),which infects the vascular bundles,blocking water and nutrient transport from roots to the aboveground.It is a severe pathogen which spreads rapidly and destroys strawberry production.Finding a way to control this disease is of great scientific value and practical importance.In this study,three fungi were isolated from the vascular tissues of sick strawberries in the field.After DNA sequencing,they were identified as Fof,Aspergillus fumigatus and Trichoderma harzianum,respectively,among which the first two are pathogens and the third is a probiotic.All fungi were controlled by thiophanate-methyl (TM),a commercial fungicide.On PDA medium,20 mg·L^(-1)5-aminolevulinic acid (ALA),a natural non-protein amino acid,promoted T.harzianum proliferation,but inhibited Fof and A.fumigatus.In confrontation test,the growth of Fof or A.fumigatus was inhibited by T.harzianum and exogenous ALA promoted T.harzianum growth but significantly inhibited the pathogen growth.When three species of fungi were separately or combinedly inoculated on healthy strawberry plants,T.harzianum promoted plant growth and development while Fof or A.fumigatus caused growth retardation,where Fof directly caused leaf yellowing and plant wilting.When the plants inoculated with different fungus were treated with ALA,the results turned out that ALA alleviated SFW symptoms by bidirectionally promoting T.harzianum proliferation and inhibiting Fof and A.fumigatus.Thus,ALA might be used in comprehensively controlling SFW in strawberry industry. 展开更多
关键词 5-Aminolevulinic acid Antagonistic effect Disease control fusarium oxysporum f.sp.fragaria(Fof) Strawberry fusarium wilt(SFW) Trichoderma harzianum
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Antifungal Activities of Eight Fungicides against Fusarium oxysporum Schlecht
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作者 Wang Dianyu Tang Minghao +1 位作者 Li Hongpeng Li Xiangguo 《Plant Diseases and Pests》 CAS 2017年第5期30-32,共3页
[Objective]The paper was to screen fungicides with strong antifungal activities against Fusarium oxysporum Schlecht,and to provide a theoretical basis for controlling root-rot of Rhodiola sachalinensis A. Bor. [Method... [Objective]The paper was to screen fungicides with strong antifungal activities against Fusarium oxysporum Schlecht,and to provide a theoretical basis for controlling root-rot of Rhodiola sachalinensis A. Bor. [Method]Using mycelial growth rate method,the indoor antifungal activities of eight fungicides against F. oxysporum were studied. [Result]Eight fungicides at different concentrations performed different antifungal activities. Toxicity test results showed that the EC_(50) value of Difenoconazole was the smallest( 4. 267 4 mg/L),followed by Mancozeb( 6. 952 5 mg/L),and the EC_(50) of Thiram,Captan and Procymidone were relatively small from 25 to 50 mg/L. [Conclusion]Among eight fungicides,Difenoconazole had the best antifungal effect against F. oxysporum,while Mancozeb,Thiram,Captan and Procymidone had good control effects as well. 展开更多
关键词 Fungicide fusarium oxysporum schlecht Antifungal activity EC_(50)
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3株非致病性镰刀菌Fusarium oxysporum菌株对番茄枯萎病的生物防治效果 被引量:17
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作者 钱晓雍 沈根祥 +3 位作者 黄丽华 顾海蓉 梁丹涛 Massimo Pugliese 《上海农业学报》 CSCD 2007年第4期60-62,共3页
对3株非致病镰刀菌Fusariumoxysporum菌株IF 23、251/2和MSA35对于番茄枯萎病的生物防治效果进行了初步研究。温室盆栽试验表明:3株菌株对番茄枯萎病均有一定的抑制作用,其中105CFU/g浓度的IF 23菌株的防治效果最好;基质灭菌对于番茄枯... 对3株非致病镰刀菌Fusariumoxysporum菌株IF 23、251/2和MSA35对于番茄枯萎病的生物防治效果进行了初步研究。温室盆栽试验表明:3株菌株对番茄枯萎病均有一定的抑制作用,其中105CFU/g浓度的IF 23菌株的防治效果最好;基质灭菌对于番茄枯萎病也有一定的抑制作用。 展开更多
关键词 非致病镰刀菌fusarium oxysporum菌株 IF 23 251/2 MSA35 番茄枯萎病 生物防治
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Screening for Siderophore-producing Endophytic Bacteria against Fusarium oxysporum 被引量:8
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作者 韩松 张守村 +1 位作者 林天兴 龚明福 《Agricultural Science & Technology》 CAS 2011年第7期994-996,共3页
[Objective] With a strain of endophytic antagonistic bacteria with good disease control efficacy in pot as trial material,the paper was to explore the antagonistic mechanism of siderophore against Fusarium oxysporum.[... [Objective] With a strain of endophytic antagonistic bacteria with good disease control efficacy in pot as trial material,the paper was to explore the antagonistic mechanism of siderophore against Fusarium oxysporum.[Method] Whether the strain produced siderophore and its fluorescent property was judged by MSA(Modified Sugar-Aspartic acid) plate.The siderophore activity of strains in liquid MSA medium was determined through the absorbance values at particular wavelength.The inhibition effects of it siderophore against F.oxysporum under different concentrations of Fe3+ were compared.Then the strain was preliminarily identified based on morphological,physiological and biochemical characteristics [Result] The fluorescent siderophore produced by the endophytic bacteria in MSA medium had a strong inhibition effect against F.oxysporum.With the increasing concentration of Fe3+,the inhibition effect against the pathogen weakened.The preliminary analysis showed these strains belonged to Bacillus.[Conclusion] Bacillus could compete the absorption of Fe3+ by secreting siderophore to inhibit the growth of F.oxysporum. 展开更多
关键词 Endophytic bacteria SIDEROPHORE fusarium oxysporum ANTAGONISM
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尖孢镰刀菌(Fusarium oxysporum Schl.)的生长特性 被引量:31
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作者 朱育菁 车建美 +3 位作者 肖荣凤 苏明星 黄晓芸 蓝江林 《中国农学通报》 CSCD 2007年第8期373-376,共4页
【研究目的】研究尖孢镰刀菌的生长特性,寻找培养过程的异质性指标。【方法】供试菌株为黄瓜尖孢镰刀菌F-H.6.5-030318-J2和花生尖孢镰刀菌F-P.5.0-030710,培养基采用PSA培养基,装液量为100ml/250ml,接菌量从培养7d的平板上打取3个6mm... 【研究目的】研究尖孢镰刀菌的生长特性,寻找培养过程的异质性指标。【方法】供试菌株为黄瓜尖孢镰刀菌F-H.6.5-030318-J2和花生尖孢镰刀菌F-P.5.0-030710,培养基采用PSA培养基,装液量为100ml/250ml,接菌量从培养7d的平板上打取3个6mm的菌片,培养温度为25±1℃,摇床转速为110r/min,培养10d,观察测定发酵液颜色、OD值、pH值、菌丝干重变化,并利用聚类分析方法划分生长阶段。【结果】在培养过程中,尖孢镰刀菌发酵液的颜色、OD值、pH值和菌丝干重都会随着培养时间而变化。在相同的培养条件下,来源于不同寄主的尖孢镰刀菌菌株之间在颜色和OD值变化存在着显著性差异,而pH值和菌丝干重变化未表现出显著性差异。来自黄瓜和花生的尖孢镰刀菌菌株的生长分为3个阶段:1 ̄2d为适应阶段,3 ̄5d为对数生长阶段,6 ̄10d为稳定阶段。【结论】尖孢镰刀菌在培养过程的色素变异和OD值的变化可作为菌株培养的表征性特征。 展开更多
关键词 尖孢镰刀菌 生长特性 PH值
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非致病性镰刀菌Fusarium oxysporum菌株47(FO47)对番茄枯萎病的防治效果 被引量:27
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作者 张琴芳 代光辉 +3 位作者 顾振芳 陈晓斌 赵杰 Alabouvette C 《上海交通大学学报(农业科学版)》 2004年第1期17-21,共5页
本实验对非致病镰刀菌Fusariumoxysporum菌株47号(简称FO47)对番茄枯萎病的生物防治效果进行了初步研究。通过对峙培养和多孔细胞培养板测试以及室内盆栽试验表明,FO47在对峙培养平板上对番茄枯萎病菌无拮抗作用;施用FO47的最佳时机是... 本实验对非致病镰刀菌Fusariumoxysporum菌株47号(简称FO47)对番茄枯萎病的生物防治效果进行了初步研究。通过对峙培养和多孔细胞培养板测试以及室内盆栽试验表明,FO47在对峙培养平板上对番茄枯萎病菌无拮抗作用;施用FO47的最佳时机是番茄播种至子叶平展期,最佳施用浓度为105~108分生孢子/毫升(简写为cfu·ml-1),防效为63.6%;结果还表明FO47液体培养的过滤液能有效抑制番茄枯萎病菌孢子的萌发,抑制萌发率为81.7%。 展开更多
关键词 非致病性镰刀菌 fusariumoxysporum菌株47 FO47 番茄枯萎病 生物防治 对峙培养 多孔细胞培养板 室内盆栽试验
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尖孢镰刀菌胡麻专化型(Fusarium oxysporum f.sp.lini)ISSR标记聚类分析 被引量:10
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作者 苑琳 刘姗姗 +3 位作者 路福平 李子钦 张辉 陈佩文 《中国油料作物学报》 CAS CSCD 北大核心 2012年第2期193-200,共8页
结合形态学特征和分子生物学方法对我国尖孢镰刀菌胡麻专化型菌株进行鉴定、聚类分析和遗传变异分析。结合传统分类方法和ITS序列分析,确定6省区96株菌株为尖孢镰刀菌。采用ISSR(简单重复序列区间)分子标记技术对这96株菌株进行分析,12... 结合形态学特征和分子生物学方法对我国尖孢镰刀菌胡麻专化型菌株进行鉴定、聚类分析和遗传变异分析。结合传统分类方法和ITS序列分析,确定6省区96株菌株为尖孢镰刀菌。采用ISSR(简单重复序列区间)分子标记技术对这96株菌株进行分析,12条引物共扩增出800个条带,多态率条带数为797条,多态率为99.62%;在相似性系数为0.88处,96株供试菌株被分为5个类群。聚类结果表明,胡麻枯萎病菌种内存在明显的多态性,且ISSR类群与地理来源存在相关性。 展开更多
关键词 尖孢镰刀菌胡麻专化型 ISSR(简单重复序列区间) 聚类分析 ITS
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甜瓜枯萎病菌(Fusarium oxysporum)专化型的初步研究 被引量:10
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作者 周小林 陈功友 +3 位作者 邵元建 蔡银杰 杨献娟 佘德琴 《植物保护》 CAS CSCD 北大核心 2008年第6期80-84,共5页
本研究获得的甜瓜枯萎病病株9株分离物(南通市6株,新疆3株),经PDA培养性状发现,其在菌落颜色、质地和生长速率方面存在差异,大型分生孢子的大小为(19.54~41.11)μm×(4.90~8.16)μm,与西瓜枯萎病菌的大型分生孢子有较大差异。胚... 本研究获得的甜瓜枯萎病病株9株分离物(南通市6株,新疆3株),经PDA培养性状发现,其在菌落颜色、质地和生长速率方面存在差异,大型分生孢子的大小为(19.54~41.11)μm×(4.90~8.16)μm,与西瓜枯萎病菌的大型分生孢子有较大差异。胚根法成株期致病性测定结果发现,本研究的甜瓜枯萎病菌分离物在不同鉴别寄主和鉴别品种上致病性存在专化型和生理小种方面的差异,但分离物中不存在西瓜枯萎病菌。利用核糖体转录间隔区保守序列设计引物,PCR检测也证明本研究甜瓜枯萎病菌不同分离物中不存在西瓜枯萎病菌。 展开更多
关键词 甜瓜 尖孢镰刀菌 致病性 专化型 小种
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分蘖洋葱鳞茎粗提物对西瓜枯萎病病原菌(Fusarium oxysporum f.sp.niveum)的影响 被引量:9
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作者 杜黎黎 王学征 +1 位作者 马鸿艳 栾非时 《东北农业大学学报》 CAS CSCD 北大核心 2011年第1期114-119,共6页
采用分蘖洋葱鳞茎粗提物对西瓜枯萎病病原菌(Fusarium oxysporum f.sp.niveum)菌丝生长、孢子萌发、病原菌生物量及产孢量等抑制作用进行研究。结果表明,分蘖洋葱鳞茎粗提物对西瓜枯萎病病原菌的菌丝生长、孢子萌发率、病原菌生物量及... 采用分蘖洋葱鳞茎粗提物对西瓜枯萎病病原菌(Fusarium oxysporum f.sp.niveum)菌丝生长、孢子萌发、病原菌生物量及产孢量等抑制作用进行研究。结果表明,分蘖洋葱鳞茎粗提物对西瓜枯萎病病原菌的菌丝生长、孢子萌发率、病原菌生物量及产孢量均有一定的抑制作用。当分蘖洋葱鳞茎粗提物浓度为1 000 mg.mL-1时,对西瓜枯萎病病原菌的抑制作用最大,抑菌率达到69.21%。探索了分蘖洋葱鳞茎粗提物防治西瓜枯萎病的可行性,为今后指导西瓜无公害生产提供理论依据。 展开更多
关键词 西瓜枯萎病 分蘖洋葱 病原菌
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厚藤共生真菌(FusariumoxysporumY24-2)胞外多糖的化学组成和结构研究 被引量:5
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作者 徐健 陶洪文 +4 位作者 陈荫 李红燕 齐晓辉 陈艳丽 毛文君 《中国海洋大学学报(自然科学版)》 CAS CSCD 北大核心 2011年第10期87-92,共6页
从厚藤共生真菌(Fusarium oxysporum Y24-2)发酵液中提取得到胞外多糖,以Q-Sepharose Fast Flow阴离子交换柱和Superdex S-75凝胶色谱柱对其进行分离纯化,得到纯度高的多糖组分F1S。采用HPGPC和HPLC对F1S的分子量及单糖组成进行分析,结... 从厚藤共生真菌(Fusarium oxysporum Y24-2)发酵液中提取得到胞外多糖,以Q-Sepharose Fast Flow阴离子交换柱和Superdex S-75凝胶色谱柱对其进行分离纯化,得到纯度高的多糖组分F1S。采用HPGPC和HPLC对F1S的分子量及单糖组成进行分析,结果表明F1S主要由Man、Glc和Gal组成,其摩尔比为1.0∶1.9∶2.1,分子量为37.3kD。F1S的GC-MS和1D,2D-NMR分析表明,该多糖是以→6)-β-Galf(1→为主链,以→2)-α-Glcp(1→,α-Glcp(1→和β-Manp(1→为支链的杂多糖,支链取代于→6)-β-Galf(1→的O-2位。该厚藤共生真菌的胞外多糖为富含呋喃型半乳糖的结构新颖的具有多分支结构的中性杂聚多糖。 展开更多
关键词 厚藤共生真菌 胞外多糖 分离纯化 化学组成 结构
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Inhibitive Activity of the Extracts of Plants in Artemisia against Fusarium oxysporum f. sp. vasinfectum and Fusarium momiliforme 被引量:2
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作者 郭艳 潘月敏 +3 位作者 高智谋 王坤 彭梅 曹舜 《Agricultural Science & Technology》 CAS 2010年第2期128-131,154,共5页
[Objective]The aim was to provide theoretical basis for the control of cotton Fusarium wilt and cotton boll rot disease.[Method]The inhibitory activity of the extracts from three species of plants(Artemisia annual L.,... [Objective]The aim was to provide theoretical basis for the control of cotton Fusarium wilt and cotton boll rot disease.[Method]The inhibitory activity of the extracts from three species of plants(Artemisia annual L.,Artemisia capillaris Thunb.,Artemisia argyi Levl.et Vant)against Fusarium oxysporum f.sp.vasinfectum and Fusarium moniliforme were studied under the condition of laboratory.[Result]The extracts of all the three plants in Artemisia showed strong antifungal activity against the tested pathogenic ... 展开更多
关键词 Artemisia extracts Antifungal activities fusarium oxysporum f.sp.vasinfectum fusarium momiliforme
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应用巢式PCR检测黄瓜尖镰孢菌(Fusarium oxysporum f.sp.cucumbrum) 被引量:1
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作者 陈微 文景芝 李永刚 《东北农业大学学报》 CAS CSCD 2007年第3期335-338,共4页
文章应用BIK2/BIK3和BIK1/BIK4两对引物,采用巢式PCR(Nested-PCR)技术对镰孢菌属26个菌株(代表了6个镰孢菌种)以及从黄瓜根际分离的20株真菌、6株细菌和7株放线菌共计59个菌株进行了扩增。结果显示,只有3个黄瓜尖镰孢菌的致病菌株能产... 文章应用BIK2/BIK3和BIK1/BIK4两对引物,采用巢式PCR(Nested-PCR)技术对镰孢菌属26个菌株(代表了6个镰孢菌种)以及从黄瓜根际分离的20株真菌、6株细菌和7株放线菌共计59个菌株进行了扩增。结果显示,只有3个黄瓜尖镰孢菌的致病菌株能产生一条长度大约为800bp的片断。对接种黄瓜尖镰孢菌的黄瓜植株进行检测结果表明,巢式PCR能从接种5d后的黄瓜病样中特异性地检测出病原菌,而症状的出现需要10~13d时间。该项技术具有较高的灵敏度,适用于黄瓜枯萎病早期侵染检测研究。 展开更多
关键词 巢式PCR 分子检测 黄瓜尖镰孢菌(fusarium oxysporum f.sp.cucumbrum) 黄瓜枯萎病
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棉花枯萎病菌(Fusarium oxysporum f.sp.Vasinfectum(ATK)Sndyder & Hansln的抑菌土初步研究 被引量:3
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作者 马存 简桂良 +4 位作者 郑传临 陈其煐 李长兴 魏庆琛 刘澍才 《棉花学报》 CSCD 北大核心 1992年第2期77-83,共7页
1987~1988年在河南新乡用相同接菌量种植感病品种,对棉花抗枯萎病品种86—1连作10年以上田块土样进行盆栽和田间小区试验。结果表明.枯萎病平均病指分别为29.4和4.4,与对照果园土比较抑菌效果分别为39.9%和85.8%。上述田块中86—1棉... 1987~1988年在河南新乡用相同接菌量种植感病品种,对棉花抗枯萎病品种86—1连作10年以上田块土样进行盆栽和田间小区试验。结果表明.枯萎病平均病指分别为29.4和4.4,与对照果园土比较抑菌效果分别为39.9%和85.8%。上述田块中86—1棉株根围5cm内土样,盆栽枯萎病平均病指19.5,抑菌效果60.5%。连作10年以上枯萎病圃土样,病指分别为27.2和6.8,抑菌效果为43.9%和76.2%。1990~1991年在辽宁省经作所试验结果,连作10年以上枯萎病圃田间抑菌效果56.2%,连作5年病圃抑菌效果17.6%。上述结果证明,枯萎病圃衰退的主要原因是棉花枯萎病菌抑菌土的产生和存在。土样内枯萎菌菌量测定结果表明,3个抑菌土土样内菌量比果园导菌土减少48.6%~59.2%。抑菌土经高压灭菌后抑菌效果完全消失,初步证明抑菌因子可能以生物因子为主。 展开更多
关键词 棉花 枯萎病菌 抑菌土 抑菌效果
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几种药剂对辣椒枯萎病菌(Fusarium oxysporum)的毒力测定 被引量:3
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作者 赵兴红 李易初 +2 位作者 于太飞 吕春梅 张俊华 《中国瓜菜》 CAS 2012年第4期19-21,共3页
采用菌丝生长速率法进行了5种药剂对辣椒枯萎病菌的毒力测定。结果表明,所采用的5种药剂均对辣椒枯萎病菌有抑制作用。药剂EC50大小顺序依次为甲霜·噁霉灵>噁霉灵>百菌清>科佳>腐霉利,其中甲霜·噁霉灵最高为19.4m... 采用菌丝生长速率法进行了5种药剂对辣椒枯萎病菌的毒力测定。结果表明,所采用的5种药剂均对辣椒枯萎病菌有抑制作用。药剂EC50大小顺序依次为甲霜·噁霉灵>噁霉灵>百菌清>科佳>腐霉利,其中甲霜·噁霉灵最高为19.4mg·L-1,腐霉利最低为5.4mg·L-1。 展开更多
关键词 辣椒枯萎病菌 镰刀菌 毒力测定
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Effects of Medium Nutrients on Fusarium oxysporum Schl. f.sp 被引量:1
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作者 苏贤岩 王齐 +2 位作者 王学峰 蒋春荣 韩金先 《Agricultural Science & Technology》 CAS 2012年第1期162-167,共6页
[Objective] To study the effects of different culture conditions on the Fusarium oxysporurn SchL f. sp. [Method] Based on species identification of the pathogenic organism of Fusarium oxysporum Schl. f. sp, effects of... [Objective] To study the effects of different culture conditions on the Fusarium oxysporurn SchL f. sp. [Method] Based on species identification of the pathogenic organism of Fusarium oxysporum Schl. f. sp, effects of different cultures and different nutrients on the mycelial growth and conidial production of Fusarium oxysporum SchL f. sp were studied. [Result] The mycelial growth and conidial pro- duction of Fusarium oxysporum SchL f. sp was different under different culture con- ditions. PDA medium was the most suitable medium for the mycelial growth and had the highest conidial production; and the mycelial grew the fastest on the medium with maltose as carbon source or peptone as nitrogen source, which also had the highest conidial production. [Conclusion] This study provided experimental basis for the study of Fusarium oxysporum SchL f. sp and also provided theoretical basis for the study and control of Fusarium oxysporum Schl. f. sp. 展开更多
关键词 fusarium oxysporum Schl f. sp MEDIUM Carbon source Nitrogen source Mycelial growth Conidial production
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