Take-all is a serious disease found in wheat across the world. Haynaldia villosa is considered to be resistant to take-all at a high level. TH3 was an amphiploid （2n =42, AABBVV） between Triticum durum and Haynaldia...Take-all is a serious disease found in wheat across the world. Haynaldia villosa is considered to be resistant to take-all at a high level. TH3 was an amphiploid （2n =42, AABBVV） between Triticum durum and Haynaldia viUosa with significant resistance to take-all fungus isolated from China. In greenhouse experiment, the derivatives of the hybrid between wheat and TH3 showed better resistance to take-all than that of the wheat control. One of the derivatives named HW918-5 was selected for further analysis. Cytological and genomic in situ hybridization （GISH） analysis indicated that a monotelosome originated from H. villosa existed in the genome of the offspring of the line HW918-5. The monotelosome with promising resistant gene for take-all was located on the 3V chromosome of H. villosa in the further PCR-based molecular analysis.展开更多
Fifty six endophytic bacteria strains were isolated from the roots of wheat,and one strain named G-32 was screened with antagonism on pathogenic fungi of Gaeumannomyces graminis var.tritici.Preliminary characterizatio...Fifty six endophytic bacteria strains were isolated from the roots of wheat,and one strain named G-32 was screened with antagonism on pathogenic fungi of Gaeumannomyces graminis var.tritici.Preliminary characterization indicated that G-32 was belonged to Bacillus cereus.The inhibitory action to the fungi was assayed in lab by using the fermentable extracts of antagonistic endophytic bacteria.The results showed that mycelium of G.graminis var.tritici growed slowly and the biomass reduced 89% compared with the control 6 d after inoculation.The hyphae were deformative which broke into pieces.Colonization was studied with dual-resistant label which suggested that the endophytic bacteria strain could colonize in the root systems of wheat.The control efficacy with G-32 strain against the disease of the wheat take-all in pot tests was better than that of common used chemical fungicide Diniconazole.展开更多
文摘Take-all is a serious disease found in wheat across the world. Haynaldia villosa is considered to be resistant to take-all at a high level. TH3 was an amphiploid （2n =42, AABBVV） between Triticum durum and Haynaldia viUosa with significant resistance to take-all fungus isolated from China. In greenhouse experiment, the derivatives of the hybrid between wheat and TH3 showed better resistance to take-all than that of the wheat control. One of the derivatives named HW918-5 was selected for further analysis. Cytological and genomic in situ hybridization （GISH） analysis indicated that a monotelosome originated from H. villosa existed in the genome of the offspring of the line HW918-5. The monotelosome with promising resistant gene for take-all was located on the 3V chromosome of H. villosa in the further PCR-based molecular analysis.
文摘Fifty six endophytic bacteria strains were isolated from the roots of wheat,and one strain named G-32 was screened with antagonism on pathogenic fungi of Gaeumannomyces graminis var.tritici.Preliminary characterization indicated that G-32 was belonged to Bacillus cereus.The inhibitory action to the fungi was assayed in lab by using the fermentable extracts of antagonistic endophytic bacteria.The results showed that mycelium of G.graminis var.tritici growed slowly and the biomass reduced 89% compared with the control 6 d after inoculation.The hyphae were deformative which broke into pieces.Colonization was studied with dual-resistant label which suggested that the endophytic bacteria strain could colonize in the root systems of wheat.The control efficacy with G-32 strain against the disease of the wheat take-all in pot tests was better than that of common used chemical fungicide Diniconazole.
