Amoeba treatment of patients suffering from pri-mary amoebic meningoencephalitis caused by Naegleria fowleri has not been successful. Dam-aged morphology and effect on genes of N. fowleri as the result of its initial ...Amoeba treatment of patients suffering from pri-mary amoebic meningoencephalitis caused by Naegleria fowleri has not been successful. Dam-aged morphology and effect on genes of N. fowleri as the result of its initial interaction with drug may provide clue to the success of treatment. In this study, we investigated the activity of chlorpromazine compared with amphotericin B and voriconazole against N.fowleri Khon Kaen strain using cell based assay and molecular techniques. Scanning electron and light micro-graph showed the drug interaction of treated amoebae with 0.098 ug/ml chlorpromazine was faster than 0.002 ug/ml amphotericin B and 12.5 ug/ml of voriconazole. The morphological cha-racteristics of treated amoebae with Gomori’s trichrome stain correlated to the scanning elec-tron microscope study. The effect of drugs to nfa1 and Mp2CL5 genes of treated amoebae found that at 120 min post exposure, chlorpromazine, voriconazole inhibited both genes except amphotericin B. Most of drug inhibited nfa1 except fluconazole. The results evaluated that chlorpromazine was higher potency and rapidly activity than amphotericin B and voriconazole against N. fowleri trophozoites.展开更多
目的:比较15个STR基因座基因频率在厦门地区大肠癌患者和正常人群中的分布,推测与大肠癌相关的基因．方法:应用PCR复合扩增结合四色荧光检测方法对血样DNA进行基因型分析,调查了本地区大肠癌患者人群和无关人群的基因频率分布,并根据二...目的:比较15个STR基因座基因频率在厦门地区大肠癌患者和正常人群中的分布,推测与大肠癌相关的基因．方法:应用PCR复合扩增结合四色荧光检测方法对血样DNA进行基因型分析,调查了本地区大肠癌患者人群和无关人群的基因频率分布,并根据二者的该15个基因座等位基因频率分布的显著性差异,推测易感连锁和抗性连锁的等位基因．结果:厦门地区大肠癌患者的D5S818(0．5200 vs 0,219 5,X2=36．69,P<0．01;RR=3．8521, P<0．05)、vWA(0．0500 vs 0．2927,X2=53．99, P<0．01;RR=0．1272,P<0．05)和FAG(0．09 vs 0．2439,X2=37．58,P<0．01:RR=0．3066, P<0．05)基因座的等位基因的分布与该地区健康人群有显著性差异,(P<0．01)．B组超微结构改变明显,而C组较B组超微结构有不同程度减轻．结论:D5S818-11附近可能存在大肠癌易感基因;vWA-15、FAG-23附近有可能存在与大肠癌相关的抗性基因．展开更多
文摘Amoeba treatment of patients suffering from pri-mary amoebic meningoencephalitis caused by Naegleria fowleri has not been successful. Dam-aged morphology and effect on genes of N. fowleri as the result of its initial interaction with drug may provide clue to the success of treatment. In this study, we investigated the activity of chlorpromazine compared with amphotericin B and voriconazole against N.fowleri Khon Kaen strain using cell based assay and molecular techniques. Scanning electron and light micro-graph showed the drug interaction of treated amoebae with 0.098 ug/ml chlorpromazine was faster than 0.002 ug/ml amphotericin B and 12.5 ug/ml of voriconazole. The morphological cha-racteristics of treated amoebae with Gomori’s trichrome stain correlated to the scanning elec-tron microscope study. The effect of drugs to nfa1 and Mp2CL5 genes of treated amoebae found that at 120 min post exposure, chlorpromazine, voriconazole inhibited both genes except amphotericin B. Most of drug inhibited nfa1 except fluconazole. The results evaluated that chlorpromazine was higher potency and rapidly activity than amphotericin B and voriconazole against N. fowleri trophozoites.
基金This work was supported by the National Natural Sciences Foundation of China (No.30340068) and the Scientific Research Foun-dation for the Returned Overseas Chinese Scholars, State Education Ministry.
文摘目的:比较15个STR基因座基因频率在厦门地区大肠癌患者和正常人群中的分布,推测与大肠癌相关的基因．方法:应用PCR复合扩增结合四色荧光检测方法对血样DNA进行基因型分析,调查了本地区大肠癌患者人群和无关人群的基因频率分布,并根据二者的该15个基因座等位基因频率分布的显著性差异,推测易感连锁和抗性连锁的等位基因．结果:厦门地区大肠癌患者的D5S818(0．5200 vs 0,219 5,X2=36．69,P<0．01;RR=3．8521, P<0．05)、vWA(0．0500 vs 0．2927,X2=53．99, P<0．01;RR=0．1272,P<0．05)和FAG(0．09 vs 0．2439,X2=37．58,P<0．01:RR=0．3066, P<0．05)基因座的等位基因的分布与该地区健康人群有显著性差异,(P<0．01)．B组超微结构改变明显,而C组较B组超微结构有不同程度减轻．结论:D5S818-11附近可能存在大肠癌易感基因;vWA-15、FAG-23附近有可能存在与大肠癌相关的抗性基因．
