Objective:To explore the mechanism of the action of Huoxue Huayu Lishui prescription on acute ankle joint injury.Methods:Forty-eight SPF rats were randomly divided into blank group,model group,Shujin Huoluo capsule gr...Objective:To explore the mechanism of the action of Huoxue Huayu Lishui prescription on acute ankle joint injury.Methods:Forty-eight SPF rats were randomly divided into blank group,model group,Shujin Huoluo capsule group,low,medium and high dose groups for promoting blood circulation and removing blood stasis.The blank group was not modeled,and the other groups were modeled.Each group of 8 animals,the Shujin Huoluo capsule group was given 0.2g/ml mixed liquid by gavage,and the low,medium and high blood circulation and blood stasis Lishui prescription group was given 1.03g/ml,2.06g/ml,4.12g/ml decoction For the stomach,the normal group was given 1.5ml/100g,once a day for 1 week.The expression of NF-κB P65 and transforming growth factor kinase 1(TAK1)protein in the soft tissue around the ankle joint of rats was detected by sports protein immunoblotting method,and the mRNA expression of NF-κB P65 and TAK1 was detected by PCR technology.Results:One day after modeling,the expressions of P65 and TAK1 mRNA and protein in the model group,the positive drug group,and low,medium and high doses of Huoxue Huayu Lishui Recipe were significantly different from those of the blank control group(P<0.05).After taking the medicine for 7 days,compared with the model group,the expressions of P65 and TAK1 mRNA and protein in the Shujin Huoluo capsule group and the low,medium and high dose Huoxue Huayu Lishui Recipe group were all decreased(P<0.05).After 7 days of taking the medicine,compared with the Shujin Huoxue Capsule group,there was no difference in P65 protein in the low-dose Huoxue Huayu Lishui Recipe group.The expression of P65 protein in the middle and high groups and the Shujin Huoxue Capsule group were all decreased(P<0.05).Compared with the Shujin Huoxue Capsule group,the expressions of TAK1 protein,P65 and TAK1 mRNA in the low,medium,and high groups and the Shujin Huoxue Capsule group were all decreased(P<0.05).The results suggest that Huoxue Huayu Lishui Recipe can inhibit the expression of NF-κB P65 and TAK1 protein in ankle joint injury.Conclusion:Huoxue Huayu Lishui prescription may regulate the expression of NF-κB P65 and TAK1 in ankle joint injury of NF-κB rats,so as to improve the clinical symptoms of local swelling and pain in the ankle joint.展开更多
Objective:To study the therapeutic effect of Huoxue Tongluo Decoction(HXTLD)on erectile dysfunction caused by ischemic stroke and identify the mechanisms involved.Methods:Network pharmacology was used to predict the k...Objective:To study the therapeutic effect of Huoxue Tongluo Decoction(HXTLD)on erectile dysfunction caused by ischemic stroke and identify the mechanisms involved.Methods:Network pharmacology was used to predict the key active ingredients and targets of HXTLD.Surgical methods were used to create a rat model of ischemic stroke.The rats were then given a suspension of HXTLD by ig administration.Erectile function was evaluated by Apomorphine(APO)induction.Real-time fluorescence quantitative reverse transcription-polymerase chain reaction(Real-time PCR)and Western blotting were used to detect the expression of related m RNAs and proteins in rat penile corpus cavernous tissue and brain tissue.Hematoxylin&Eosin(HE)staining was used to investigate structural changes in the penile cavernous tissue.Results:Network pharmacology showed that tumor necrosis factor(TNF),nitric oxide synthase 3(eNOS),and vascular endothelial growth factor(VEGF)were the key targets of HXTLD in the treatment of erectile dysfunction caused by ischemic stroke.Experimental studies showed that HXTLD improved erectile dysfunction caused by ischemic stroke.HE results showed that HXTLD improved the structure of the corpus cavernosa.HXTLD also inhibited the expression of TNF and VEGF proteins in penile tissue(P<0.05)and enhanced the expression of e NOS protein in penile tissue(P<0.05).Conclusion:HXTLD improved the erectile function of rats with erectile dysfunction caused by ischemic stroke by regulating the m RNA and protein levels of TNF,e NOS and VEGF.展开更多
目的探讨自拟中药温肾活血利水方治疗糖尿病神经源性膀胱(DNB)的临床疗效。方法选择邯郸市第一医院2014年1月—2015年12月收治的DNB患者82例,按照数字随机表法分为2组,每组41例,对照组在常规治疗基础上给予硫酸锌注射液和甲钴胺胶囊治疗...目的探讨自拟中药温肾活血利水方治疗糖尿病神经源性膀胱(DNB)的临床疗效。方法选择邯郸市第一医院2014年1月—2015年12月收治的DNB患者82例,按照数字随机表法分为2组,每组41例,对照组在常规治疗基础上给予硫酸锌注射液和甲钴胺胶囊治疗,治疗组在对照组治疗基础上给予中药温肾活血利水方治疗,疗程4周,比较2组治疗前后尿流动力学指标(包括残尿量、最大膀胱容积、充盈末逼尿肌压力、最大尿流率)、空腹血糖(FBG)、餐后2 h血糖(2 h PG)、糖化血红蛋白(Hb A1C)和尿8-羟基脱氧鸟苷(8-Ohd G),并比较2组治疗总有效率。结果治疗后治疗组与对照组比较,治疗组残尿量减少(P<0.01),充盈末逼尿肌压力降低(P<0.01),最大膀胱容积和最大尿流率增加(P<0.05,P<0.01);治疗组FBG、2 h PG、Hb A1C及尿8-Ohd G水平低于对照组(P<0.05,P<0.01),治疗总有效率高于对照组(P<0.05),差异均有统计学意义。结论自拟温肾活血利水方可有效改善DNB患者尿流动力学指标,其机制可能与改善氧化应激状态有关。展开更多
基金Hunan Provincial Traditional Chinese Medicine Scientific Research Program Project(No.201920)National Natural Science Foundation of China(No.81874476)Hunan Natural Science Foundation Project(No.2018JJ2303)。
文摘Objective:To explore the mechanism of the action of Huoxue Huayu Lishui prescription on acute ankle joint injury.Methods:Forty-eight SPF rats were randomly divided into blank group,model group,Shujin Huoluo capsule group,low,medium and high dose groups for promoting blood circulation and removing blood stasis.The blank group was not modeled,and the other groups were modeled.Each group of 8 animals,the Shujin Huoluo capsule group was given 0.2g/ml mixed liquid by gavage,and the low,medium and high blood circulation and blood stasis Lishui prescription group was given 1.03g/ml,2.06g/ml,4.12g/ml decoction For the stomach,the normal group was given 1.5ml/100g,once a day for 1 week.The expression of NF-κB P65 and transforming growth factor kinase 1(TAK1)protein in the soft tissue around the ankle joint of rats was detected by sports protein immunoblotting method,and the mRNA expression of NF-κB P65 and TAK1 was detected by PCR technology.Results:One day after modeling,the expressions of P65 and TAK1 mRNA and protein in the model group,the positive drug group,and low,medium and high doses of Huoxue Huayu Lishui Recipe were significantly different from those of the blank control group(P<0.05).After taking the medicine for 7 days,compared with the model group,the expressions of P65 and TAK1 mRNA and protein in the Shujin Huoluo capsule group and the low,medium and high dose Huoxue Huayu Lishui Recipe group were all decreased(P<0.05).After 7 days of taking the medicine,compared with the Shujin Huoxue Capsule group,there was no difference in P65 protein in the low-dose Huoxue Huayu Lishui Recipe group.The expression of P65 protein in the middle and high groups and the Shujin Huoxue Capsule group were all decreased(P<0.05).Compared with the Shujin Huoxue Capsule group,the expressions of TAK1 protein,P65 and TAK1 mRNA in the low,medium,and high groups and the Shujin Huoxue Capsule group were all decreased(P<0.05).The results suggest that Huoxue Huayu Lishui Recipe can inhibit the expression of NF-κB P65 and TAK1 protein in ankle joint injury.Conclusion:Huoxue Huayu Lishui prescription may regulate the expression of NF-κB P65 and TAK1 in ankle joint injury of NF-κB rats,so as to improve the clinical symptoms of local swelling and pain in the ankle joint.
基金supported by“3+3”Project for the Inheritance of Beijing Traditional Chinese Medicine(No.2016-SZ-C-60)。
文摘Objective:To study the therapeutic effect of Huoxue Tongluo Decoction(HXTLD)on erectile dysfunction caused by ischemic stroke and identify the mechanisms involved.Methods:Network pharmacology was used to predict the key active ingredients and targets of HXTLD.Surgical methods were used to create a rat model of ischemic stroke.The rats were then given a suspension of HXTLD by ig administration.Erectile function was evaluated by Apomorphine(APO)induction.Real-time fluorescence quantitative reverse transcription-polymerase chain reaction(Real-time PCR)and Western blotting were used to detect the expression of related m RNAs and proteins in rat penile corpus cavernous tissue and brain tissue.Hematoxylin&Eosin(HE)staining was used to investigate structural changes in the penile cavernous tissue.Results:Network pharmacology showed that tumor necrosis factor(TNF),nitric oxide synthase 3(eNOS),and vascular endothelial growth factor(VEGF)were the key targets of HXTLD in the treatment of erectile dysfunction caused by ischemic stroke.Experimental studies showed that HXTLD improved erectile dysfunction caused by ischemic stroke.HE results showed that HXTLD improved the structure of the corpus cavernosa.HXTLD also inhibited the expression of TNF and VEGF proteins in penile tissue(P<0.05)and enhanced the expression of e NOS protein in penile tissue(P<0.05).Conclusion:HXTLD improved the erectile function of rats with erectile dysfunction caused by ischemic stroke by regulating the m RNA and protein levels of TNF,e NOS and VEGF.
文摘目的探讨自拟中药温肾活血利水方治疗糖尿病神经源性膀胱(DNB)的临床疗效。方法选择邯郸市第一医院2014年1月—2015年12月收治的DNB患者82例,按照数字随机表法分为2组,每组41例,对照组在常规治疗基础上给予硫酸锌注射液和甲钴胺胶囊治疗,治疗组在对照组治疗基础上给予中药温肾活血利水方治疗,疗程4周,比较2组治疗前后尿流动力学指标(包括残尿量、最大膀胱容积、充盈末逼尿肌压力、最大尿流率)、空腹血糖(FBG)、餐后2 h血糖(2 h PG)、糖化血红蛋白(Hb A1C)和尿8-羟基脱氧鸟苷(8-Ohd G),并比较2组治疗总有效率。结果治疗后治疗组与对照组比较,治疗组残尿量减少(P<0.01),充盈末逼尿肌压力降低(P<0.01),最大膀胱容积和最大尿流率增加(P<0.05,P<0.01);治疗组FBG、2 h PG、Hb A1C及尿8-Ohd G水平低于对照组(P<0.05,P<0.01),治疗总有效率高于对照组(P<0.05),差异均有统计学意义。结论自拟温肾活血利水方可有效改善DNB患者尿流动力学指标,其机制可能与改善氧化应激状态有关。
