Senecavirus A(SVA)has a positive-sense,single-stranded RNA genome.Its 5´untranslated region harbors an internal ribosome entry site(IRES),comprising 10 larger or smaller stem-loop structures(including a pseudokno...Senecavirus A(SVA)has a positive-sense,single-stranded RNA genome.Its 5´untranslated region harbors an internal ribosome entry site(IRES),comprising 10 larger or smaller stem-loop structures(including a pseudoknot)that have been demonstrated to be well conserved.However,it is still unclear whether each stem-loop subdomain,such as a single stem or loop,is also highly conserved.To clarify this issue in the present study,a set of 29 SVA cDNA clones were constructed by site-directed mutagenesis(SDM)on the IRES.The SDM-modified scenarios included:(1)stem-formed complementary sequences exchanging with each other;(2)loop transversion;(3)loop transition;and(4)point mutations.All cDNA clones were separately transfected into cells for rescuing viable viruses,whereas only four SVAs of interest could be recovered,and were genetically stable during 20 passages.One progeny grew significantly slower than the other three did.The dual-luciferase reporter assay showed that none of the SDM-modified IRESes significantly inhibited the IRES activity.Our previous study indicated that a single motif from any of the ten stem structures,if completely mutated,would cause the failure of virus recovery.Interestingly,our present study revealed three stem structures,whose individual complementary sequences could exchange with each other to rescue sequence-modifying SVAs.Moreover,one apical loop was demonstrated to have the ability to tolerate its own full-length transition,also having no impact on the recovery of sequence-modifying SVA.The present study suggested that not every stem-loop structure was strictly conserved in its conformation,while the full-length IRES itself was well conserved.This provides a new research direction on interaction between the IRES and many factors.展开更多
Wireless Power Transfer(WPT)technology can provide real-time power for many terminal devices in Internet of Things(IoT)through millimeterWave(mmWave)to support applications with large capacity and low latency.Although...Wireless Power Transfer(WPT)technology can provide real-time power for many terminal devices in Internet of Things(IoT)through millimeterWave(mmWave)to support applications with large capacity and low latency.Although the intelligent reflecting surface(IRS)can be adopted to create effective virtual links to address the mmWave blockage problem,the conventional solutions only adopt IRS in the downlink from the Base Station(BS)to the users to enhance the received signal strength.In practice,the reflection of IRS is also applicable to the uplink to improve the spectral efficiency.It is a challenging to jointly optimize IRS beamforming and system resource allocation for wireless energy acquisition and information transmission.In this paper,we first design a Low-Energy Adaptive Clustering Hierarchy(LEACH)clustering protocol for clustering and data collection.Then,the problem of maximizing the minimum system spectral efficiency is constructed by jointly optimizing the transmit power of sensor devices,the uplink and downlink transmission times,the active beamforming at the BS,and the IRS dynamic beamforming.To solve this non-convex optimization problem,we propose an alternating optimization(AO)-based joint solution algorithm.Simulation results show that the use of IRS dynamic beamforming can significantly improve the spectral efficiency of the system,and ensure the reliability of equipment communication and the sustainability of energy supply under NLOS link.展开更多
Exploring effective iridium(Ir)-based electrocatalysts with stable iridium centers is highly desirable for oxygen evolution reaction(OER).Herein,we regulated the incorporation manner of Ir in Co_(3)O_(4)support to sta...Exploring effective iridium(Ir)-based electrocatalysts with stable iridium centers is highly desirable for oxygen evolution reaction(OER).Herein,we regulated the incorporation manner of Ir in Co_(3)O_(4)support to stabilize the Ir sites for effective OER.When anchored on the surface of Co_(3)O_(4)in the form of Ir(OH)_6 species,the created Ir-OH-Co interface leads to a limited stability and poor acidic OER due to Ir leaching.When doped into Co_(3)O_(4)lattice,the analyses of X-ray absorption spectroscopy,in-situ Raman,and OER measurements show that the partially replacement of Co in Co_(3)O_(4)by Ir atoms inclines to cause strong electronic effect and activate lattice oxygen in the presence of Ir-O-Co interface,and simultaneously master the reconstruction effect to mitigate Ir dissolution,realizing the improved OER activity and stability in alkaline and acidic environments.As a result,Ir_(lat)@Co_(3)O_(4)with Ir loading of 3.67 wt%requires 294±4 mV/285±3 mV and 326±2 mV to deliver 10 mA cm^(-2)in alkaline(0.1 M KOH/1.0 M KOH)and acidic(0.5 M H_(2)SO_(4))solution,respectively,with good stability.展开更多
In this paper,we consider mobile edge computing(MEC)networks against proactive eavesdropping.To maximize the transmission rate,IRS assisted UAV communications are applied.We take the joint design of the trajectory of ...In this paper,we consider mobile edge computing(MEC)networks against proactive eavesdropping.To maximize the transmission rate,IRS assisted UAV communications are applied.We take the joint design of the trajectory of UAV,the transmitting beamforming of users,and the phase shift matrix of IRS.The original problem is strong non-convex and difficult to solve.We first propose two basic modes of the proactive eavesdropper,and obtain the closed-form solution for the boundary conditions of the two modes.Then we transform the original problem into an equivalent one and propose an alternating optimization(AO)based method to obtain a local optimal solution.The convergence of the algorithm is illustrated by numerical results.Further,we propose a zero forcing(ZF)based method as sub-optimal solution,and the simulation section shows that the proposed two schemes could obtain better performance compared with traditional schemes.展开更多
人体呼吸系统相关疾病常常伴随着呼吸深度和节律的异常,因此呼吸信号监测和呼吸模式识别在医疗健康领域中尤其是对于睡眠监测、疾病预断具有重要意义。其中,非接触式的脉冲式超宽带雷达(Impulse Radio Ultra-Wideband,IR-UWB)因具有良...人体呼吸系统相关疾病常常伴随着呼吸深度和节律的异常,因此呼吸信号监测和呼吸模式识别在医疗健康领域中尤其是对于睡眠监测、疾病预断具有重要意义。其中,非接触式的脉冲式超宽带雷达(Impulse Radio Ultra-Wideband,IR-UWB)因具有良好的距离分辨率和穿透能力以及全天候全天时、安全无创的检测优势,正逐步成为睡眠健康监护领域中最关键的感知技术之一。然而受睡眠监测特定的室内场景影响,复杂的测量环境给呼吸模式特征的准确提取带来了限制和挑战,传统的雷达呼吸模式识别算法主要关注一维呼吸时、频域特征,而IR-UWB雷达目标回波信息分散在多个距离门内,使用一维特征识别准确率较低。为此,本文针对IR-UWB雷达中人体呼吸在时间上慢速起伏运动、在距离上是扩展目标的信号模型特点,提出了一种引入时距信息的IR-UWB雷达多域特征融合呼吸模式识别方法。算法在提取一维呼吸信号波形时、频域特征的基础上更进一步挖掘雷达二维时距图像中潜在的呼吸模式形态特征,通过多域特征融合实现呼吸模式的非接触式检测和识别。在图像处理上,针对图像受呼吸异常节律影响呈现局部粘连特性导致呼吸周期提取难的问题,提出一种通过相位矩阵图像处理来检测雷达图像中的呼吸时距条带从而获取图像特征的方法。实验结果表明,利用该算法提取的多域特征对六种呼吸模式进行机器学习的分类识别,可以实现96.3%的识别准确率。展开更多
基金This work was supported by the National Natural Science Found ation of China(32273000)the Qingdao Demonstration Project for People-benefit from Science and Techniques,China(23-2-8-xdny-14nsh and 24-2-8-xdny-4-nsh)+1 种基金the National Program of Undergraduate Innovation and Entrepreneurship,China(202310435039)the Open Project Fund of State Key Laboratory of Microbial Technology,China(M2023-03)。
文摘Senecavirus A(SVA)has a positive-sense,single-stranded RNA genome.Its 5´untranslated region harbors an internal ribosome entry site(IRES),comprising 10 larger or smaller stem-loop structures(including a pseudoknot)that have been demonstrated to be well conserved.However,it is still unclear whether each stem-loop subdomain,such as a single stem or loop,is also highly conserved.To clarify this issue in the present study,a set of 29 SVA cDNA clones were constructed by site-directed mutagenesis(SDM)on the IRES.The SDM-modified scenarios included:(1)stem-formed complementary sequences exchanging with each other;(2)loop transversion;(3)loop transition;and(4)point mutations.All cDNA clones were separately transfected into cells for rescuing viable viruses,whereas only four SVAs of interest could be recovered,and were genetically stable during 20 passages.One progeny grew significantly slower than the other three did.The dual-luciferase reporter assay showed that none of the SDM-modified IRESes significantly inhibited the IRES activity.Our previous study indicated that a single motif from any of the ten stem structures,if completely mutated,would cause the failure of virus recovery.Interestingly,our present study revealed three stem structures,whose individual complementary sequences could exchange with each other to rescue sequence-modifying SVAs.Moreover,one apical loop was demonstrated to have the ability to tolerate its own full-length transition,also having no impact on the recovery of sequence-modifying SVA.The present study suggested that not every stem-loop structure was strictly conserved in its conformation,while the full-length IRES itself was well conserved.This provides a new research direction on interaction between the IRES and many factors.
基金supported by the National Natural Science Foundation of China 62001051.
文摘Wireless Power Transfer(WPT)technology can provide real-time power for many terminal devices in Internet of Things(IoT)through millimeterWave(mmWave)to support applications with large capacity and low latency.Although the intelligent reflecting surface(IRS)can be adopted to create effective virtual links to address the mmWave blockage problem,the conventional solutions only adopt IRS in the downlink from the Base Station(BS)to the users to enhance the received signal strength.In practice,the reflection of IRS is also applicable to the uplink to improve the spectral efficiency.It is a challenging to jointly optimize IRS beamforming and system resource allocation for wireless energy acquisition and information transmission.In this paper,we first design a Low-Energy Adaptive Clustering Hierarchy(LEACH)clustering protocol for clustering and data collection.Then,the problem of maximizing the minimum system spectral efficiency is constructed by jointly optimizing the transmit power of sensor devices,the uplink and downlink transmission times,the active beamforming at the BS,and the IRS dynamic beamforming.To solve this non-convex optimization problem,we propose an alternating optimization(AO)-based joint solution algorithm.Simulation results show that the use of IRS dynamic beamforming can significantly improve the spectral efficiency of the system,and ensure the reliability of equipment communication and the sustainability of energy supply under NLOS link.
基金supported by the National Natural Science Foundation of China(52150410409).
文摘Exploring effective iridium(Ir)-based electrocatalysts with stable iridium centers is highly desirable for oxygen evolution reaction(OER).Herein,we regulated the incorporation manner of Ir in Co_(3)O_(4)support to stabilize the Ir sites for effective OER.When anchored on the surface of Co_(3)O_(4)in the form of Ir(OH)_6 species,the created Ir-OH-Co interface leads to a limited stability and poor acidic OER due to Ir leaching.When doped into Co_(3)O_(4)lattice,the analyses of X-ray absorption spectroscopy,in-situ Raman,and OER measurements show that the partially replacement of Co in Co_(3)O_(4)by Ir atoms inclines to cause strong electronic effect and activate lattice oxygen in the presence of Ir-O-Co interface,and simultaneously master the reconstruction effect to mitigate Ir dissolution,realizing the improved OER activity and stability in alkaline and acidic environments.As a result,Ir_(lat)@Co_(3)O_(4)with Ir loading of 3.67 wt%requires 294±4 mV/285±3 mV and 326±2 mV to deliver 10 mA cm^(-2)in alkaline(0.1 M KOH/1.0 M KOH)and acidic(0.5 M H_(2)SO_(4))solution,respectively,with good stability.
基金This work was supported by the Key Scientific and Technological Project of Henan Province(Grant Number 222102210212)Doctoral Research Start Project of Henan Institute of Technology(Grant Number KQ2005)Key Research Projects of Colleges and Universities in Henan Province(Grant Number 23B510006).
文摘In this paper,we consider mobile edge computing(MEC)networks against proactive eavesdropping.To maximize the transmission rate,IRS assisted UAV communications are applied.We take the joint design of the trajectory of UAV,the transmitting beamforming of users,and the phase shift matrix of IRS.The original problem is strong non-convex and difficult to solve.We first propose two basic modes of the proactive eavesdropper,and obtain the closed-form solution for the boundary conditions of the two modes.Then we transform the original problem into an equivalent one and propose an alternating optimization(AO)based method to obtain a local optimal solution.The convergence of the algorithm is illustrated by numerical results.Further,we propose a zero forcing(ZF)based method as sub-optimal solution,and the simulation section shows that the proposed two schemes could obtain better performance compared with traditional schemes.
文摘人体呼吸系统相关疾病常常伴随着呼吸深度和节律的异常,因此呼吸信号监测和呼吸模式识别在医疗健康领域中尤其是对于睡眠监测、疾病预断具有重要意义。其中,非接触式的脉冲式超宽带雷达(Impulse Radio Ultra-Wideband,IR-UWB)因具有良好的距离分辨率和穿透能力以及全天候全天时、安全无创的检测优势,正逐步成为睡眠健康监护领域中最关键的感知技术之一。然而受睡眠监测特定的室内场景影响,复杂的测量环境给呼吸模式特征的准确提取带来了限制和挑战,传统的雷达呼吸模式识别算法主要关注一维呼吸时、频域特征,而IR-UWB雷达目标回波信息分散在多个距离门内,使用一维特征识别准确率较低。为此,本文针对IR-UWB雷达中人体呼吸在时间上慢速起伏运动、在距离上是扩展目标的信号模型特点,提出了一种引入时距信息的IR-UWB雷达多域特征融合呼吸模式识别方法。算法在提取一维呼吸信号波形时、频域特征的基础上更进一步挖掘雷达二维时距图像中潜在的呼吸模式形态特征,通过多域特征融合实现呼吸模式的非接触式检测和识别。在图像处理上,针对图像受呼吸异常节律影响呈现局部粘连特性导致呼吸周期提取难的问题,提出一种通过相位矩阵图像处理来检测雷达图像中的呼吸时距条带从而获取图像特征的方法。实验结果表明,利用该算法提取的多域特征对六种呼吸模式进行机器学习的分类识别,可以实现96.3%的识别准确率。
