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High Temperature Effects on Yeast-like Endosymbiotes and Pesticide Resistance of the Small Brown Planthopper, Laodelphax striatellus 被引量:1
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作者 ZHANG Xiao-jie YU Xiao-ping CHEN Jian-ming 《Rice science》 SCIE 2008年第4期326-330,共5页
The newly-hatched nymphs of the small brown planthopper (SBPH), Laodelphax striatellus, including field and sensitive populations, were subjected to the high-temperature (35°C) treatment. The number of yeast-like... The newly-hatched nymphs of the small brown planthopper (SBPH), Laodelphax striatellus, including field and sensitive populations, were subjected to the high-temperature (35°C) treatment. The number of yeast-like endosymbiotes in SBPH reduced by 23.47%–34.23%, 57.86%–61.51% and 88.96%–90.71% after the high-temperature treatment for 1 d, 2 d, and 3 d, respectively. However, the size of yeast-like endosymbiotes was not obviously affected. Resistance of SBPH to three insecticides (imidacloprid, chlorpyrifos and fipronil) decreased with the increase of treatment time. 展开更多
关键词 laodelphax striatellus yeast-like endosymbiote high temperature pesticide resistance insect pest
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Identification and characterization of a TLR13 gene homologue from Laodelphax striatellus involved in the immune response induced by rice stripe virus
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作者 ZHOU Xue HU Jia +5 位作者 FU Mei-li JIN Ping ZHANG Yun-ye XIANG Ying LI Yao MA Fei 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第1期183-192,共10页
Toll-like receptors(TLRs) are the critical superfamily homologues that initiate sensing of the invasion of pathogens by the Toll pathway. As one of several intracellular nucleic acid-sensing TLRs, TLR13 is activated b... Toll-like receptors(TLRs) are the critical superfamily homologues that initiate sensing of the invasion of pathogens by the Toll pathway. As one of several intracellular nucleic acid-sensing TLRs, TLR13 is activated by an unmethylated motif present in the large ribosomal subunit of bacterial RNA. However, little attention has been paid to the function of TLR13 gene homologue from Laodelphax striatellus(designated as LsToll-13) in the immune response to rice stripe virus(RSV). Herein, LsToll-13 was cloned and characterized using RACE-PCR. Phylogenetic analysis showed that LsToll-13 was clustered with the TLR13 from six insects. Real-time PCR analysis demonstrated that the expression level of LsToll-13 was significantly reduced in L. striatellus with RSV infection compared with that in the naive strain. When the expression of LsToll-13 was significantly up-regulated at 6 h after bacterial infection, the expression of ribonucleoprotein(RNP) indicated that the RSV titer in the host insect was significantly suppressed. Upon knockdown of LsToll-13, using RNA interference(RNAi) in L. striatellus, the expression level of RNP was significantly increased with enhanced RSV accumulation, suggesting that LsToll-13 potentially protects L. striatellus from RSV infection. Taken together, our results indicated that LsToll-13 might be involved in the immune response of L. striatellus to RSV infection, and provided a new insight into further elucidating the molecular mechanisms of complex pathogen-host interactions and integrative pest management. 展开更多
关键词 laodelphax striatellus RSV TLR13 RNAI antiviral immunity
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NDUFA8 potentially rescues Wolbachia-induced cytoplasmic incompatibility in Laodelphax striatellus
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作者 Jie Chen Meng-Ke Wang +3 位作者 Qi-Xian Xie Xiao-Li Bing Tong-Pu Li Xiao-Yue Hong 《Insect Science》 SCIE CSCD 2023年第6期1689-1700,共12页
The endosymbiont Wolbachia manipulates host reproduction by several strategies, one of the most important of which is cytoplasmic incompatibility (CI). CI can be rescued when Wolbachia-infected males mate with females... The endosymbiont Wolbachia manipulates host reproduction by several strategies, one of the most important of which is cytoplasmic incompatibility (CI). CI can be rescued when Wolbachia-infected males mate with females infected with the same Wolbachia strain. However, the potential rescue mechanism of CI in the small brown planthopper Laodelphax striatellus is unclear. In this study, comparative transcriptome analysis was applied to explore the effect of Wolbachia on L. striatellus eggs. A total of 1387 differentially expressed genes were identified. RNA interference of 7 Wolbachia-upregulated key planthopper genes reduced egg reproduction, suggesting that Wolbachia might improve fecundity in L. striatellus by affecting these 7 genes. Suppressing the expression of another upregulated gene, NDUFA8 (encoding NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 8-like) by RNA interference significantly increased the mortality of early embryos without affecting the number of deposited eggs. Wolbachia infection upregulated the mRNA level of NDUFA8, and dsNDUFA8 treatment of Wolbachia-infected females recreated CI-like symptoms, suggesting that NDUFA8 is associated with the rescue phenotype. Because all L. striatellus populations worldwide are infected with Wolbachia, NDUFA8 is a potential pest control target. 展开更多
关键词 cytoplasmic incompatibility laodelphax striatellus NDUFA8 reproduc-tion RESCUE WOLBACHIA
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Identification of a fatty acid synthase gene(FAS1)from Laodelphax striatellus planthoppers contributing to fecundity
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作者 Jia-Yan Cheng Pei-Han Yu +5 位作者 Xue Xia Ru Zhang Li-Hua Wang Ji-Chao Fang Ary AHoffmann Guang-Hua Luo 《Insect Science》 SCIE CAS CSCD 2023年第3期599-610,共12页
Fatty acid synthase (FAS) is a multifunctional enzyme that plays an important role in the formation of fatty acids. The fatty acids take part in many processes, such as cell signaling and energy metabolism, and in ins... Fatty acid synthase (FAS) is a multifunctional enzyme that plays an important role in the formation of fatty acids. The fatty acids take part in many processes, such as cell signaling and energy metabolism, and in insects they are important in both cuticular hydrocarbon (CHC) formation and reproduction. Here we characterized the sequence structure and function of an FAS from the small brown planthopper (SBPH), Laodelphax striatellus. The full-length open reading frame (ORF) sequence of LsFAS1 was 7122 bp, encoding a predicted protein of 2373 amino acid residues. There were 7 functional domains in the LsFAS1 protein sequence. Gene expression screening by real-time quantitative polymerase chain reaction (RT-qPCR) showed that LsFAS1 was expressed in all developmental stages. Relative expression was highest at the 4th-instar and female adult stages. Among different tissues, the expression level of LsFAS1 in the ovary was the highest. Phylogenetic analysis showed that LsFAS1 clustered in a clade with 2 FASs from Nilaparvata lugens. Furthermore, these 3 FASs are related to cockroach BgFAS and locust LmFAS. After RNA interference-mediated knock-down, most treated insects died at eclosion. In addition, the lifespan of dsFAS1-treated female adults was shorter than that of the dsGFP-injected control, and offspring production decreased. Also, the expression of vitellogenin (Vg) and vitellogenin receptor (VgR) genes decreased. Virgin females dissected at days 2 and 4 post-eclosion showed many matured oocytes in planthoppers treated with dsGFP but not with dsFAS1. These data highlight the importance of LsFAS1 in SBPH, including a role in reproduction. 展开更多
关键词 fatty acid synthase FECUNDITY laodelphax striatellus RNA interference sequence analysis
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Effect of Rice Sowing Date on Occurrence of Small Brown Planthopper and Epidemics of Planthopper-Transmitted Rice Stripe Viral Disease 被引量:8
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作者 ZHU Jin-liang ZHU Zeng-rong +6 位作者 ZHOU Yin LU Qiang SUN Xiang-liang TAO Xian-guo CHEN Yue WANG Hua-di CHENG Jia-an 《Agricultural Sciences in China》 CAS CSCD 2009年第3期332-341,共10页
To understand the relationship between rice sowing date and occurrence of the rice small brown planthopper (SBPH) Laodelphax striatellus Fallen and the epidemics of the planthopper-transmitted rice stripe viral (RS... To understand the relationship between rice sowing date and occurrence of the rice small brown planthopper (SBPH) Laodelphax striatellus Fallen and the epidemics of the planthopper-transmitted rice stripe viral (RSV) disease, four sowing dates of rice were evaluated in 2006 and 2007. The results showed that the peak density of SBPH and RSV incidence in the nursery and in the transplanted field decreased with the delay of sowing date in single crop of japonica rice in north Zhejiang Province of China. The relationship between seedling RSV incidence at the end of the nursery trial with sowing date was well described by Weibull equation. The area under the curve of population dynamics (AUCPD or planthopper-day accumulation) or the peak density of the planthopper in the nursery could be summarized by a logistic equation. RSV incidence in the transplanted fields could be characterized quantitatively by a multivariate regression equation, including the variables of sowing date, peak density of the vector, and RSV incidence at the end of the nursery trial. That the descriptive model excluded the AUCPD in transplanted field implies that this variable is not necessary in forecasting disease epidemics in the field. The 2-year experiments sufficiently indicated that suitable sowing of rice could be used as one of the effective measures to control the vector population and therefore the planthopper-transmitted RSV on a larger scale. The optimal sowing date for the single-cropped transplanted japonica rice is recommended from late May to early June in north Zhejiang, China. 展开更多
关键词 RICE sowing date laodelphax striatellus rice stripe viral disease EPIDEMICS
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Antibiotic exposure perturbs the bacterial community in the small brown planthopper Laodelphax striatellus 被引量:3
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作者 Xu Zhang Tong-Pu Li +5 位作者 Chun-Ying Zhou Dian-Shu Zhao Yu-Xi Zhu Xiao-Li Bing Hai-Jian Huang Xiao-Yue Hong 《Insect Science》 SCIE CAS CSCD 2020年第5期895-907,共13页
Bacteria symbionts in herbivores play an important role in host biology and ecology,and are affected by environmental factors such as temperature,diet,habitat,antibiotics and so on.However,the effects of antibiotics o... Bacteria symbionts in herbivores play an important role in host biology and ecology,and are affected by environmental factors such as temperature,diet,habitat,antibiotics and so on.However,the effects of antibiotics on the microbiome of the small brown planthopper Laodelphax striatellus(SBPH)remain unclear.Here,we studied the effects of tetracycline on the diversity and composition of bacterial colonies in different tissues of SBPH using high throughput sequencing of 16S ribosomal RNA amplicons.Our results show that Proteobacteria,Firmicutes,Bacteroidetes,Actinobacteria were most abundant in SBPH,and the genera Asaia and Wolbachia were most abundant in all body parts of SBPH.Antibiotic treatment had persistent effects on the composition of the SBPH microbiome.Tetracycline depleted the population of Firmicutes,Bacteroidetes,Tenericutes and Fusobacteria,and nearly 100%eliminated Wolbachia,Bacteroides and Abiotrophia in SBPH.Together,these results suggest that antibiotic exposure affects the bacteria symbionts of different body parts in SBPH and will facilitate future studies of the bacterial symbionts of arthropod hosts. 展开更多
关键词 ANTIBIOTICS GUT laodelphax striatellus microbial structure OVARY
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Molecular cloning and characterization of the putative Halloween gene Phantom from the small brown planthopper Laodelphax striatellus 被引量:1
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作者 Shuang Jia Pin-Jun Wan Guo-Qing Li 《Insect Science》 SCIE CAS CSCD 2015年第6期707-718,共12页
Ecdysteroid hormone 20-hydroxyecdysone plays fundamental roles in in- sect postembryonic development and reproduction. Several cytochrome P450 mono- oxygenases (CYPs), encoded by the Halloween genes, have been docum... Ecdysteroid hormone 20-hydroxyecdysone plays fundamental roles in in- sect postembryonic development and reproduction. Several cytochrome P450 mono- oxygenases (CYPs), encoded by the Halloween genes, have been documented to be involved in ecdysteroidogenesis in representative insects in Diptera, Lepidoptera and Orthoptera. Here the putative Halloween gene Phantom (Phm, cyp306al) from a hemipteran insect species, the small brown planthopper Laodelphax striatellus, was cloned. LsPHM shows five insect conserved P450 motifs, that is, Helix-C, Helix-I, Helix-K, PERF and heme- binding motifs. Temporal and spatial expression patterns of LsPhm were evaluated by quantitative polymerase chain reaction. Through the fourth-instar and the early fifth-instar stages, LsPhm showed two expression peaks in day 2 and days 4-5 fourth-instar nymphs, and three troughs in day 1 and 3 fourth instars and day 1 fifth instars. On day 5 of the fourth-instar nymphs, LsPhm clearly had a high transcript level in the thorax where the prothoracic glands were located. Dietary introduction of double-stranded RNA (dsRNA) of LsPhm at the nymph stage successfully knocked down the target gene, decreased expres- sion level ofecdysone receptor (LsEcR) gene and caused a higher nymphal mortality rate and delayed development. Ingestion of 20-hydroxyecdysone on LsPhm-dsRNA-exposed nymphs did not increase LsPhm expression level, but almost completely rescued the LsEcR mRNA level, and relieved the negative effects on survival and development. Thus, our data suggest that the putative LsPhm encodes a ftmctional 25-hydroxylase that catalyzes the biosynthesis of ecdysteroids in L. striatellus. 展开更多
关键词 double-stranded RNA ECDYSTEROIDOGENESIS laodelphax striatellus perfor-mance PHANTOM
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Laodelphax striatellus Atg8 facilitates Rice stripe virus infection in an autophagy-independent manner 被引量:1
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作者 Yuan-Ling Yu Meng-Ting Zhang +5 位作者 Yan Huo Ji-Liang Tang Qing Liu Xiao-Ying Chen Rong-Xiang Fang Li-Li Zhang 《Insect Science》 SCIE CAS CSCD 2021年第2期315-329,共15页
Rice stripe virus(RSV)is the causative agent of rice stripe disease and is completely dependent on insect vectors for its plant-to-plant transmission.Laodelphax striatellus is the major insect vector for RSV.In this s... Rice stripe virus(RSV)is the causative agent of rice stripe disease and is completely dependent on insect vectors for its plant-to-plant transmission.Laodelphax striatellus is the major insect vector for RSV.In this study,we explored the interactions be-tween RSV infection and L.striatellus autophagy,a potential intrinsic antiviral mechanism in insects.We found that L.striatellus autophagic activity did not affect RSV infection;however,the autophagy related-8(Atg8)gene significantly enhanced virus infection.Dur-ing RSV initial infection within the L.striatellus midgut,silencing of Atg8 expression significantly decreased the phosphorylation of c-Jun N-terminal kinase(p-JNK);however,when RSV infection is absent,silencing of Atg8 did not alter p-JNK levels.Thesc results indicated that Atg8 might activate the JNK machinery by allowing more virus infection into cells.We further revealed that Atg8-deficiency significantly decreased RSV accumu-lation on the surface of the insect midgut epithelial cells,suggesting a receptor trafficking function of the y-aminobutyric acid receptor-associated protein family.Using the RSV ovary entry as a model,in which vitellogenin receptor(V gR)mediates RSV cell entry,we clarified that Atg8-deficiency decreased the abundance of V gR localizing on the cytomem-brane and disturbed the attachment of RSV in the germarium zones.Collectively,these results revealed an autophagy-independent function of L.striatellus Atg8 that enhances RSV initial infection by increasing virus attachment on the infection sites. 展开更多
关键词 Atg8 insect autophagy laodelphax striatellus receptor trafficking Rice stripe virus viral initial infection
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High temperature determines the ups and downs of small brown planthopper Laodelphax striatellus population
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作者 Xiang-Dong Liu Ai-Min Zhang 《Insect Science》 SCIE CAS CSCD 2013年第3期385-392,共8页
Small brown planthopper, Laodelphax striatellus (Fall6n) numbers usually drop sharply in the summer and revive quickly in the autumn. However, it is unclear whether and how the high temperature plays a role in this ... Small brown planthopper, Laodelphax striatellus (Fall6n) numbers usually drop sharply in the summer and revive quickly in the autumn. However, it is unclear whether and how the high temperature plays a role in this process. The effects of durations of heat exposure (33~C) on life-history traits were examined here. Exposure of adults for 1 day during the oviposition stage led to a very low survival of nymphs. The average longevity ofL. striatellus exposed for 1-31 days from oviposition was significantly longer than that of the control (27℃). Short-term (1-5 days) heat exposure of the third instar nymphs did not significantly influence eclosion, but exposure of the fourth instar nymphs significantly increased eclosion. Lifespan from egg to adult was significantly lengthened when the third instar nymphs were exposed to heat for 2-15 days, or the fourth instar were exposed for 10 days. The preoviposition period was prolonged by heat exposure of the third or fourth instar nymphs. Short-term heat exposure of less than 3 days of the third or fourth instar nymphs did not restrict fecundity, but when the exposure duration exceeded 5 days the total eggs per female and hatchability decreased. Exposure to high temperature increased the brachypter rate of adults. In summary, low survival and slowing development under heat exposure resulted in population decline in the summer, and the relatively high fecundity and brachypter rate led to quick revival in autumn. Temperature in the summer determines the rise and fall in numbers ofL. striatellus. 展开更多
关键词 development FECUNDITY heat exposure laodelphax striatellus wing form
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The Empidemiology of Wheat Rosette Stunt in Hulunbeir District of Inner-Mongolia
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作者 Zhang Minghou, Lu Jun, Liu Hongyi(Northeast Agricultural University, Harbin 150030 PRC) 《Journal of Northeast Agricultural University(English Edition)》 CAS 1995年第2期96-103,共8页
The virus disease threatening wheat production in Hulunbcir District of Inner - Mongolia was identified as NCMV (Northern Cereal Mosaic Virus) or WRSV (Wheat Rosette Stunt Virus) by tests of host range, transmission, ... The virus disease threatening wheat production in Hulunbcir District of Inner - Mongolia was identified as NCMV (Northern Cereal Mosaic Virus) or WRSV (Wheat Rosette Stunt Virus) by tests of host range, transmission, virus particle and scrology. The main vector for spreading the virus is planthopper, Laodelphax striatellus (Fallen), which overwinters in the stubbles of wheat underground. The overwintered virulifcrous nymphs emerged in late April is responsible for the early infection of the disease. Agropyron repens, an important perennial weed in cultivated regions, is also an important wild host of the virus and its vector. Severe loss is induced when wheat is infected before 3 - leaf stage. The disease incidence could be predicted by a model with the population and virulifcrous rate of overwintered vectors as independent variables. 展开更多
关键词 NCMV (Northern Cereal Mosaic Virus) WRSV (Wheat Ro-sette Stunt Virus) Apropyron repens laodelphax striatellus(Fallen)
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Similarities and spatial variations of bacterial and fungal communities in field rice planthopper (Hemiptera: Delphacidae) populations 被引量:1
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作者 Xiao-Li Bing Dian-Shu Zhao +2 位作者 Chang-Wu Peng Hai-Jian Huang Xiao-Yue Hong 《Insect Science》 SCIE CAS CSCD 2020年第5期947-963,共17页
Rice planthoppers are notorious plant sap-feeding pests which cause serious damage.While several microbes in rice planthoppers have been broadly characterized,the abundance and diversity of bacteria and fungi in field... Rice planthoppers are notorious plant sap-feeding pests which cause serious damage.While several microbes in rice planthoppers have been broadly characterized,the abundance and diversity of bacteria and fungi in field planthoppers are largely unknown.This study investigated the bacterial and fungal community compositions of Chinese wild rice planthoppers Laodelphax striatellus and Sogatella furcifera using parallel 16S rRNA gene amplicon and internal transcribed space region sequencing.The bacteria varied sig-nificantly between the species and were partitioned significantly by sex,tissues and host environments in each species.The majority of bacteria were affiliated with the genera Wol-bachia,Cardinium,Rickettsia and Pantoea.The abundance of Wolbachia was negatively correlated with that of Cardinium in both planthopper species.Compared with bacteria,the abundance and diversity of fiungi did not differ between sexes but both were enriched in the gut.The bacterial community as a whole showed no significant correlation with the fungal community.The majority of fungi were related to Sarocldium,Alternaria,Malasseria,Aspergillus and Curvularia.A phylogenetic analysis revealed that these fungi were closely related to botanic symbionts or pathogens.Our results provide novel insights into the bacteria and fungi of rice planthoppers. 展开更多
关键词 fungal diversity laodelphax striatellus MICROBIOTA Sogatella furcifera PLANTHOPPERS
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