Immunoperoxidase histochemical assay with monoclonal antibody against human cytomaglovirus (HCMV) was used to detect immediate early antigen (IEA) and early antigen (EA) of HCMV infection in liver tissue of 72 podiatr...Immunoperoxidase histochemical assay with monoclonal antibody against human cytomaglovirus (HCMV) was used to detect immediate early antigen (IEA) and early antigen (EA) of HCMV infection in liver tissue of 72 podiatric cases (34 autopsies and 38 biopsies). The HCMV antigen was positive in 25 % (18/27). Among them, 12 cases were both HCMV-IEA and EA positive; 4 were HCMV-IEA positive and 2 HCMV-EA positive only. Liver HCMV infection rate in neonates, the infants with the age <1 year and >l year was 8. 0 %,60. 0 %, and 14. 8 %, respectively, indicating that liver HCMV infectioh occurred at various ages. The liver HCMV infection rate in different diseases was 50. 0 % in infantile hepatitis syndrome; 70. 0 % in extrabiliary malformation, and 12. 5 % in other hepatopathies, suggesting that infantile hepatitis syndrome and extrabiliary malformation were related with HCMV infection in liver tissues.展开更多
The mammalian liver is a morphologically and functionally complex organ, made up of not only of the largely predominant parenchymal cells (hepatocytes) but also non-parenchymal cells. Although there are less non-paren...The mammalian liver is a morphologically and functionally complex organ, made up of not only of the largely predominant parenchymal cells (hepatocytes) but also non-parenchymal cells. Although there are less non-parenchymal cells than hepatocytes, they nevertheless play an important role in regulating many hepatocyte functions, as well as in the immunology of the liver. We investigated the structural aspects of the liver and the morpho-functional characteristics of Ito and Kupffer cells in two domesticated ruminant species (cattle and goat) in comparison with four wild ruminant species living in captivity in a zoo in northern Italy. The liver specimens were studied using histological, histochemical and immunohistochemical methods. The liver parenchyma was structurally normal. Immunohistochemistry was performed for desmin, glial fibrillary acidic protein (GFAP), vimentin, α-smooth muscle actin (α-SMA), collagen I, lysozyme, CD 68 and tumor necrosis factor α (TNF-α). In all the studied ruminants, Ito cells reacted with desmin and vimentin antibodies, Kupffer cells were evidenced only with lysozyme-immunopositivity, and both displayed a characteristic distribution in the hepatic lobular/acinar structure. The results obtained, not only contribute to the knowledge of ruminant wild species, but also help to define a normal structure reference for the diagnosis and treatment of liver diseases.展开更多
文摘Immunoperoxidase histochemical assay with monoclonal antibody against human cytomaglovirus (HCMV) was used to detect immediate early antigen (IEA) and early antigen (EA) of HCMV infection in liver tissue of 72 podiatric cases (34 autopsies and 38 biopsies). The HCMV antigen was positive in 25 % (18/27). Among them, 12 cases were both HCMV-IEA and EA positive; 4 were HCMV-IEA positive and 2 HCMV-EA positive only. Liver HCMV infection rate in neonates, the infants with the age <1 year and >l year was 8. 0 %,60. 0 %, and 14. 8 %, respectively, indicating that liver HCMV infectioh occurred at various ages. The liver HCMV infection rate in different diseases was 50. 0 % in infantile hepatitis syndrome; 70. 0 % in extrabiliary malformation, and 12. 5 % in other hepatopathies, suggesting that infantile hepatitis syndrome and extrabiliary malformation were related with HCMV infection in liver tissues.
文摘The mammalian liver is a morphologically and functionally complex organ, made up of not only of the largely predominant parenchymal cells (hepatocytes) but also non-parenchymal cells. Although there are less non-parenchymal cells than hepatocytes, they nevertheless play an important role in regulating many hepatocyte functions, as well as in the immunology of the liver. We investigated the structural aspects of the liver and the morpho-functional characteristics of Ito and Kupffer cells in two domesticated ruminant species (cattle and goat) in comparison with four wild ruminant species living in captivity in a zoo in northern Italy. The liver specimens were studied using histological, histochemical and immunohistochemical methods. The liver parenchyma was structurally normal. Immunohistochemistry was performed for desmin, glial fibrillary acidic protein (GFAP), vimentin, α-smooth muscle actin (α-SMA), collagen I, lysozyme, CD 68 and tumor necrosis factor α (TNF-α). In all the studied ruminants, Ito cells reacted with desmin and vimentin antibodies, Kupffer cells were evidenced only with lysozyme-immunopositivity, and both displayed a characteristic distribution in the hepatic lobular/acinar structure. The results obtained, not only contribute to the knowledge of ruminant wild species, but also help to define a normal structure reference for the diagnosis and treatment of liver diseases.
