In vivo anticancer activity of maesopsin 4—O— β—glucoside isolated from leaves of Artocarpus tonkinensis A.Chev.Ex Gagnep

Objective:To investigate the antitumor effect of maesopsin 4-O-β-glucoside(TAT2) isolated from the leaves of Artocarpus tonkinensis(A.tonkinensis) A.Chev.ex Gagnep.Methods:The antitumor activity of TAT2 was evaluated in Lewis lung carcinoma(LLC) tumor-bearing mice.BALB/c mice had tumors induced by implantation with 2× 10~6 LLC cells into the subcutaneous right posterior Hank.Tumor-bearing mice were treated orally with a range of doses of TAT2 and a standard drug,doxorubicin.Animals were observed for tumor growth and mortality rate.Blood was collected to determine hematological and biochemical parameters.Results TAT2 was isolated from an ethanolic extract of 1.tonkinensis leaves.Its structure was determined by MS and NMR spectroscopy,and identified as TAT2.The compound did not show acute toxicity at the highest dose tested(2 OIK) mg/kg body weight).TAT2 exhibited antitumor activity by decreasing tumor growth,increasing the survival rale,and ameliorating some hematological and biochemical parameters at doses of 100 and 200 mg/kg body weight(P<0.05).Conclusions:These results indicate that TAT2 possesses clear antitumor activity.Due to its bioavailability and low toxicity,and the fact that it could be isolated in a large scale from A.tonkinensis leaves,the compound shows promise as a potential anticancer drug.

藏药“生等”中墨沙酮成分的超临界CO_2萃取工艺研究

用超临界CO2流体萃取法对藏药“生等”（RhamnellagilgilitcaMansf．etMelch．）的主要成分墨沙酮（Maesopsin）的萃取进行了研究，探讨了压力、温度、夹带剂流量对革取效率的影响；并用HPLC法作了含量测定．结果表明，本法提取率约为超声波军取法的1．2倍。

西藏猫乳活性成分研究

首次从西藏猫乳中分离并鉴定了３个化合物，它们是Ｍａｅｓｏｐｓｉｎ（Ⅰ），山奈酚（Ｋａｅｍｐｆｅｒｏｌ，Ⅱ）和右旋肌醇甲醚（Ｐｉｎｉｔｏｌ，Ⅲ）．利用二维核磁对（Ⅰ）和（Ⅲ）的全部氢信号和碳信号进行了确切归属，并由此而确定了（Ⅲ）的立体构型。化合物（Ⅰ）为其中主要成分。初步筛选结果显示，化合物（Ⅰ）有较强的抗癌促进因子作用和细胞毒作用。

高效液相色谱法测定藏药生等中墨沙酮的含量

用反相高效液相色谱法测定藏药生等心材中墨沙酮的含量。浸泡和超声提取相结合，ＯＤＳ柱，甲醇水（２８∶７２），内含００５ｍｏｌ／Ｌ磷酸二氢钾（ｐＨ４）为流动相，ＵＶ２９０ｎｍ检测。回收率为９８９％。墨沙酮含量为１０２％。