Background:Bionovation's CSFA800 is a new automated digital cell imaging analyzer.We evaluated the performance of the CSFA800 by comparing it with artificial peripheral blood white blood cell counting.Methods:Acco...Background:Bionovation's CSFA800 is a new automated digital cell imaging analyzer.We evaluated the performance of the CSFA800 by comparing it with artificial peripheral blood white blood cell counting.Methods:According to inclusion and exclusion criteria,131 randomly selected samples(77 abnormal samples and 54 normal samples)were compared.Correlations between automated and manual counting results were analyzed.Manual counting was carried out according to the guidelines of the Association of Clinical and Laboratory Standards.Results:Counts of neutrophils,lymphocytes,monocytes,eosinophils,baso-phils,and immature granulocytes obtained from CSFA800 and artificial methods were linearly and positively correlated,with R values of 0.73,0.65,0.24,0.2,0.4,and 0.63,respectively,all p<0.05.Therefore,correlations be-tween CSFA800 and manual counting are acceptable.Compared with the DI‐60 Automated Digital Cell Morphology System(DI‐60;Sysmex),CSFA800 is more efficient and can analyze 20,000 cells in 1 min.However,the overall accuracy of CSFA800 is not as good as DI‐60,although its counting perfor-mance is better for basophils.Conclusions:The performance of CSFA800 for WBC counts is acceptable,and it displayed good performance for neutrophils,lymphocytes,and imma-ture granulocytes.Compared to DI‐60,CSFA800 is more efficient but has slightly lower overall accuracy.To some extent,CSFA800 is helpful to opti-mize the clinical laboratory workflow and improve the working efficiency of inspectors.展开更多
High-speed stirred mills are utilized to grind particles below 10mm. Grinding sulphide minerals to as low as 10mm achieve adequate mineral liberation for successful downstream mineral processing operations, such as fl...High-speed stirred mills are utilized to grind particles below 10mm. Grinding sulphide minerals to as low as 10mm achieve adequate mineral liberation for successful downstream mineral processing operations, such as flotation and leaching. Particle breakage mechanism such as fracture or abrasion, determines the morphological surface features of the product particles. It is anticipated that particles, which break along grain boundaries (intergranular) produce rough surfaces, whereas particles that break across the grain boundaries (transgranular) possess smoother surfaces. In this study, particles are ground in a stirred mill and their morphological features were analyzed using automated and manual detection methods. Literature and conventional belief are that high-speed stirred mills break particles by attrition. This paper showed that fracture is also an important breakage mechanism along with attrition. Breakage mechanism is a factor of input stress intensity, in the form of the mill agitator speed, and type of mineral. It is observed that at higher agitator speed galena fractures along the grain boundaries, whereas quartz, abrade across the grain boundaries.展开更多
BACKGROUND Spontaneous bacterial peritonitis(SBP)is one of the most important complications of patients with liver cirrhosis entailing high morbidity and mortality.Making an accurate early diagnosis of this infection ...BACKGROUND Spontaneous bacterial peritonitis(SBP)is one of the most important complications of patients with liver cirrhosis entailing high morbidity and mortality.Making an accurate early diagnosis of this infection is key in the outcome of these patients.The current definition of SBP is based on studies performed more than 40 years ago using a manual technique to count the number of polymorphs in ascitic fluid(AF).There is a lack of data comparing the traditional cell count method with a current automated cell counter.Moreover,current international guidelines do not mention the type of cell count method to be employed and around half of the centers still rely on the traditional manual method.AIM To compare the accuracy of polymorph count on AF to diagnose SBP between the traditional manual cell count method and a modern automated cell counter against SBP cases fulfilling gold standard criteria:Positive AF culture and signs/symptoms of peritonitis.METHODS Retrospective analysis including two cohorts:Cross-sectional(cohort 1)and case-control(cohort 2),of patients with decompensated cirrhosis and ascites.Both cell count methods were conducted simultaneously.Positive SBP cases had a pathogenic bacteria isolated on AF and signs/symptoms of peritonitis.RESULTS A total of 137 cases with 5 positive-SBP,and 85 cases with 33 positive-SBP were included in cohort 1 and 2,respectively.Positive-SBP cases had worse liver function in both cohorts.The automated method showed higher sensitivity than the manual cell count:80%vs 52%,P=0.02,in cohort 2.Both methods showed very good specificity(>95%).The best cutoff using the automated cell counter was polymorph≥0.2 cells×10^(9)/L(equivalent to 200 cells/mm^(3))in AF as it has the higher sensitivity keeping a good specificity.CONCLUSION The automated cell count method should be preferred over the manual method to diagnose SBP because of its higher sensitivity.SBP definition,using the automated method,as polymorph cell count≥0.2 cells×10^(9)/L in AF would need to be considered in patients admitted with decompensated cirrhosis.展开更多
基金2019 Medical Big Data and Artificial Intelligence R&D Project,Grant/Award Number:2019MBD‐048Special Funding for Clinical Research of Wu Jieping Medical Foundation,Grant/Award Number:320.6750.18173。
文摘Background:Bionovation's CSFA800 is a new automated digital cell imaging analyzer.We evaluated the performance of the CSFA800 by comparing it with artificial peripheral blood white blood cell counting.Methods:According to inclusion and exclusion criteria,131 randomly selected samples(77 abnormal samples and 54 normal samples)were compared.Correlations between automated and manual counting results were analyzed.Manual counting was carried out according to the guidelines of the Association of Clinical and Laboratory Standards.Results:Counts of neutrophils,lymphocytes,monocytes,eosinophils,baso-phils,and immature granulocytes obtained from CSFA800 and artificial methods were linearly and positively correlated,with R values of 0.73,0.65,0.24,0.2,0.4,and 0.63,respectively,all p<0.05.Therefore,correlations be-tween CSFA800 and manual counting are acceptable.Compared with the DI‐60 Automated Digital Cell Morphology System(DI‐60;Sysmex),CSFA800 is more efficient and can analyze 20,000 cells in 1 min.However,the overall accuracy of CSFA800 is not as good as DI‐60,although its counting perfor-mance is better for basophils.Conclusions:The performance of CSFA800 for WBC counts is acceptable,and it displayed good performance for neutrophils,lymphocytes,and imma-ture granulocytes.Compared to DI‐60,CSFA800 is more efficient but has slightly lower overall accuracy.To some extent,CSFA800 is helpful to opti-mize the clinical laboratory workflow and improve the working efficiency of inspectors.
文摘High-speed stirred mills are utilized to grind particles below 10mm. Grinding sulphide minerals to as low as 10mm achieve adequate mineral liberation for successful downstream mineral processing operations, such as flotation and leaching. Particle breakage mechanism such as fracture or abrasion, determines the morphological surface features of the product particles. It is anticipated that particles, which break along grain boundaries (intergranular) produce rough surfaces, whereas particles that break across the grain boundaries (transgranular) possess smoother surfaces. In this study, particles are ground in a stirred mill and their morphological features were analyzed using automated and manual detection methods. Literature and conventional belief are that high-speed stirred mills break particles by attrition. This paper showed that fracture is also an important breakage mechanism along with attrition. Breakage mechanism is a factor of input stress intensity, in the form of the mill agitator speed, and type of mineral. It is observed that at higher agitator speed galena fractures along the grain boundaries, whereas quartz, abrade across the grain boundaries.
文摘BACKGROUND Spontaneous bacterial peritonitis(SBP)is one of the most important complications of patients with liver cirrhosis entailing high morbidity and mortality.Making an accurate early diagnosis of this infection is key in the outcome of these patients.The current definition of SBP is based on studies performed more than 40 years ago using a manual technique to count the number of polymorphs in ascitic fluid(AF).There is a lack of data comparing the traditional cell count method with a current automated cell counter.Moreover,current international guidelines do not mention the type of cell count method to be employed and around half of the centers still rely on the traditional manual method.AIM To compare the accuracy of polymorph count on AF to diagnose SBP between the traditional manual cell count method and a modern automated cell counter against SBP cases fulfilling gold standard criteria:Positive AF culture and signs/symptoms of peritonitis.METHODS Retrospective analysis including two cohorts:Cross-sectional(cohort 1)and case-control(cohort 2),of patients with decompensated cirrhosis and ascites.Both cell count methods were conducted simultaneously.Positive SBP cases had a pathogenic bacteria isolated on AF and signs/symptoms of peritonitis.RESULTS A total of 137 cases with 5 positive-SBP,and 85 cases with 33 positive-SBP were included in cohort 1 and 2,respectively.Positive-SBP cases had worse liver function in both cohorts.The automated method showed higher sensitivity than the manual cell count:80%vs 52%,P=0.02,in cohort 2.Both methods showed very good specificity(>95%).The best cutoff using the automated cell counter was polymorph≥0.2 cells×10^(9)/L(equivalent to 200 cells/mm^(3))in AF as it has the higher sensitivity keeping a good specificity.CONCLUSION The automated cell count method should be preferred over the manual method to diagnose SBP because of its higher sensitivity.SBP definition,using the automated method,as polymorph cell count≥0.2 cells×10^(9)/L in AF would need to be considered in patients admitted with decompensated cirrhosis.