Microsatellite Variation Within Three Populations of Inbred C57BL/6J Strain

The goal of this study was to investigate the genetic stability of the C57BL/6J （B6） inbred mouse strain maintained in different breeders. Three populations of B6, Popl and Pop2 purchased from Beijing and Pop3 purchased from Shanghai, were examined. Fifteen microsatellite loci reported to be polymorphic among inbred strains were amplified using FAM labeled primers and genotyped with ABI Prism 377 automated sequencer. Seven loci were found polymorphic, and all the loci were homozygous in all the three populations. The present study indicates that genetic variation occurs in different B6 populations although they are still inbred in each breeder. The mechanism of genetic variation is not well understood now, but it is very important to know the precise content of the B6 genome before use of this strain in research.

A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice

Dengue is a significant public health concern across tropical and subtropical regions worldwide,principally causing disease in children.Very young children are at increased risk of severe manifestations of dengue infection.The mechanism of dengue disease in this population is not fully understood.In this study,we present a murine model of dengue virus primary infection in suckling C57BL/6 and BALB/c mice in order to investigate disease pathogenesis.Three-day-old C57BL/6 mice intraperitoneally infected with DENV-2 NGC were more susceptible to infection than BALB/c mice,showing increased liver enzymes,extended viremia,dissemination to organs and histological alterations in liver and small intestine.Furthermore,the immune response in DENV-infected C57BL/6 mice exhibited a marked Th1 bias compared to BALB/c mice.These findings highlight the possibility of establishing an immunocompetent mouse model of DENV-2 infection in suckling mice that reproduces certain signs of disease observed in humans and that could be used to further study agerelated mechanisms of dengue pathogenesis.

EFFECTS OF MODERATE NOISE EXPOSURE ON HEARING FUNCTION IN C57BL/6J MICE

Objective To study characteristics of hearing loss after exposure to moderate noise exposure in C57BL/6J mice. Methods Male C57BL/6J mice with normal hearing at age of 5-6 weeks were chosen for this study. The mice were randomly sclccted to be studied immediately after exposure （Group P0）, or 1 day （Group P1）, 3 days （Group P3）, 7 day （Group P7） or 14 days （P14） after exposure. Their before exposure condition served as the normal control. All mice were exposed to a broad-band white noise at 100 dB SPL for 2 hours, ABR thresholds were used to estimate hearing status at each time point. Results ABR threshold elevation was seen at every tested frequency at P0 （P〈0.01）. Elevation at high-frequencies （16 kHz and 32 kHz） was greater than at lower frequencies （4 kHz and 8 kHz, P〈0.05）. From P1 to P14, ABR thresholds continuously improved, and there was no significant difference between P14 and before exposure （P〉0.05）. Conclusion There is a frequency specific re- sponse to 100 dB SPL broad-band white noise in C57BL/6J mice, with the high-frequency being more susceptible. Hearing loss induced by moderate noise exposure appears reversible in C57BL/6J mice.

Anti-leukemic and anti-angiogenic effects of D-Limonene on K562-implanted C57BL/6 mice and the chick chorioallantoic membrane model

Background: D-Limonene, a monoterpene from citrus fruit has been found to have chemopreventive and chemotherapeutic activities in various types of cancers. In this study, we evaluated the in vivo effect of D-Limonene on a K562-induced model of chronic myeloid leukemia(CML) in C57 BL/6 mice.Method: The tail vein injection model of K562 cells in immunocompromised C57 BL/6 mice was developed and evaluated for characteristics of the disease. The mice were treated with D-Limonene and evaluated for haematological parameters. We also evaluated the effect of D-Limonene on angiogenesis using the chick chorioallantoic membrane(CAM) assay.Results: In a complete blood count, a significant dose-dependent reduction in white blood cell, neutrophil and lymphocyte counts, but an elevation in red blood cell count and haemoglobin content was observed with D-Limonene treatment compared to the disease control or untreated group. In the CAM assay, D-Limonene produced a significant dose-dependent reduction in number of blood vessels in treatment groups compared to the vehicle-treated group.Conclusion: These studies suggest promising anti-leukemic and anti-angiogenic effects of D-Limonene in the treatment of CML.

Differences in action potential propagation speed and axon initial segment plasticity between neurons from Sprague-Dawley rats and C57BL/6 mice

Action potentials(APs)in neurons are generated at the axon initial segment(AIS).AP dynamics,including initiation and propagation,are intimately associated with neuronal excitability and neurotransmitter release kinetics.Most learning and memory studies at the single-neuron level have relied on the use of animal models,most notably rodents.Here,we studied AP initiation and propagation in cultured hippocampal neurons from Sprague-Dawley(SD)rats and C57BL/6(C57)mice with genetically encoded voltage indicator(GEVI)-based voltage imaging.Our data showed that APs traveled bidirectionally in neurons from both species;forward-propagating APs(fpAPs)had a different speed than backpropagating APs(bpAPs).Additionally,we observed distinct AP propagation characteristics in AISs emerging from the somatic envelope compared to those originating from dendrites.Compared with rat neurons,mouse neurons exhibited higher bpAP speed and lower fpAP speed,more distally located ankyrin G(AnkG)in AISs,and longer Nav1.2 lengths in AISs.Moreover,during AIS plasticity,AnkG and Nav1.2 showed distal shifts in location and shorter lengths of labeled AISs in rat neurons;in mouse neurons,however,they showed a longer AnkG-labeled length and more distal Nav1.2 location.Our findings suggest that hippocampal neurons in SD rats and C57 mice may have different AP propagation speeds,different AnkG and Nav1.2 patterns in the AIS,and different AIS plasticity properties,indicating that comparisons between these species must be carefully considered.

Ultraviolet-attenuated cercariae ofSchistosoma japonicum fail to effectively induce a Th1 response in spite of up-regulating expression of cytotoxicity-related genes in C57BL/6 mice

Objective： To better understand the reason that Schistosoma japonicurn （S. japonicum） ultraviolet （UV）- radiated cercariae could not induce high level of protection in C57BL/6 mice. Methods： Microarray technology was performed to investigate the gene transcription profile in skin draining lymph nodes （sdLNs） at 1 w after exposure to attenuated cercariae （AC） or normal cercariae （NC） of S. japonicum in C57BL/6 mice. The expressions of some representative genes were further confirmed by real-time PCR. Subsequently, the expressions of Th1/Th2 cytokine genes, cytotoxicity-related genes, as well as co-stimulator genes in spleens from AC-vaccinated and NC- infected mice were analyzed by real-time PCR at w 3 and 6 post-exposure. Results： The gene expressions of Th1 cytokines, including interferon-y （IFN-γ）, interleukin （IL）-12 and tumor necrosis factor-α （TNF-α） in the sdLNs were significantly lower in AC-vaccinated mice than in NC-infected mice. Furthermore, the gene expressions of Th1- and Th2- cytokines, including IFN-γ, IL-12, TNF-α, IL-4 and IL-10, in the spleens from AC-vaccinated mice showed little changes at w 3 and 6 post-vaccination. In addition, cytotoxicity-related molecules including granzyme A, granzyme B, granzyme K, perforin 1 and Fas L were up-regulated from the early stage of vaccination, and peaked at the 3rd w after vaccination with UV-AC. Conclusion： UV-AC of S. japonicum could not ef- fectively induce a Thl response in C57BL/6 mice, which may be an explanation for the low protection against parasite challenge, and the role played by up-regulated expression of cytotoxicity-related genes in mice needs to be further investigated.

Different sources of MSCs on pulmonary fibrosis in C57BL/6 mice

Since stem cell therapy is the most effective treatment in the field of tissue reparation and reconstitution,the present study aimed to explore the different sources of mesenchymal stem cells(MSCs)on the different effects of pulmonary fibrosis-related cytokines in C57BL/6 mice.For reaching this goal,we isolated MSCs from umbilical cord blood and placenta and used for stem cell therapy in a mouse model of pulmonary fibrosis model.The pulmonary fibrosis model was done by injecting bleomycin into the trachea of C57BL/6 mice.Then we assessed the degree of pulmonary fibrosis in each mouse lung tissue at weeks 1,2,3,and 4.In addition,flow cytometry was used to evaluate the frequency of CD73,CD90,CD106,CD34,CD45,CD14 cells at the mononuclear cell level;and western blotting assays revealed the expression of IκB-α.Our results showed that stem cell therapy by placenta-derived MSC had a lower level of CD34,CD45,CD14 cells at the mononuclear cell level,and that improved pulmonary fibrosis at both molecular and pathological levels.In addition,western blotting assays revealed that the expression of IκB-αwas down-regulated in MSC-treated animals.In addition,placenta-derived MSC was the most effective in improving pulmonary fibrosis in comparison to other sources.This study suggests that MSC might be a novel therapeutic approach in pulmonary fibrosis due to an enhanced anti-inflammatory effect.Also,MSC modification by gene editing could enhance their therapeutic effect in mouse pulmonary fibrosis.

Dietary Effect of Squalene on Lipid Metabolism of Obese/Diabetes KK-<i>A^y</i>Mice and Wild-Type C57BL/6J Mice

The purpose of this study was to evaluate the influence of squalene (SQ) on plasma and hepatic lipid levels of obese/diabetic KK-Ay mice and wild-type C57BL/6J mice. SQ supplementation significantly increased the HDL cholesterol of KK-Ay mice, which was paralleled with no significant difference in the total and non-HDL cholesterol levels. The increase in HDL cholesterol was also found in the plasma of normal C57BL/6J mice, but the difference was not significant. SQ administration significantly increased neutral lipids (NL) in the liver of KK-Ay mice, while no significant difference was observed in the polar lipids and the total cholesterol levels. The increase in NL was primarily due to the increase in TAG. However, the cholesterol level significantly increased due to SQ intake in the liver of C57BL/6J mice, while no significant difference was found in other lipid levels. The present study suggests that SQ may effectively increase HDL cholesterol level, an important anti-atherosclerotic factor, especially in subjects with metabolic disorders.

C57BL/6 and DBA/1 Mice Differ in Their Response to Supplementation with 1,25D and Paricalcitol

Active vitamin D (1,25D) is a fat-soluble vitamin that is mainly produced in the skin by the conversion of 7-dehydrocholesterol under ultraviolet light stimulation.Its role in calcium homeostasis,bone growth, and prevention of rickets and osteomalacia has been known for over two hundred years.Its

Characterization of Endophytes Isolated from <i>Eclipta prostrate</i>and Roles in Regulating the Gut Microbiota of C57BL/6J Mice

Eclipta prostrata has been extensively studied for its promoting effect on human health. Previous study proved that E. prostrata possessed anti-osteoporosis function in SAMP6 mice via gut microbiota (GM). Endophytes are widespread in plants, especially in Chinese herbal medicines. However, little is known regarding the endophytes of E. prostrata. In this study, we focus on screening and identifying the endophytes from plant E. prostrata and exploring their roles in modulating GM. According to biochemical, physiological tests and 16S rDNA sequence analysis, ten endophytes were characterized in different locations of plant E. prostrata belonging to Lactococcus, Bacillus, Enterococcus, Exiguobacterium and Pantoea. The antimicrobial activity of endophytes EP01-10 was investigated via the oxford cup method. Furthermore, the acid and bile salt resistant ability of EP01-10 was detected to explore their survival ability in gastrointestinal tract. Results indicated that strains of Lactococcus, Bacillus and Exiguobacterium (EP01, 03 and 05) showed strong antimicrobial activity against Escherichia coli and Staphylococcus aureus and strains of Lactococcus, Bacillus and Enterococcus (EP01, 02, 04, 08 and 10) have the ability to survive in the gastrointestinal tract. 16S rRNA sequencing of GM in C57BL/6J mice was performed for diversity and structure analysis responded to the administration of E. prostrata extract. E. prostrata extract acted on improving the microflora diversity, particularly in increasing the abundance of Lactococcus significantly. Thus, as an endophyte, Lactococcus plays an important role in E. prostrata modulating intestinal tract. Our study suggested that endophytes of Chinese herbal medicines might be a novel target for the treatment of diseases by regulating the GM.

Expression ofα2-Na/K-ATPase in C57BL/6J Mice Inner Ear and Its Relationship with Age-related Hearing Loss

K^(+)cycling in the cochlea is critical to maintain hearing.Many sodium-potassium pumps are proved to participate in K^(+)cycling,such as Na/K-ATPase.Theα2-Na/K-ATPase is an important isoform of Na/K-ATPase.The expression ofα2-Na/K-ATPase in the cochlea is not clear.In this study,we used C57BL/6 mice as a model of presbycusis and implemented immunohistochemistry staining and quantitative real time-PCR,and theα2-Na/K-ATPase expression pattern was confirmed in the inner ear.It was foundα2-Na/K-ATPase was expressed widely in cochlea and its mRNA and protein expression was gradually reduced with aging(4-,14-,26-and 48-weeks old mice).We suspected that,the down-regulation ofα2-Na/K-ATPase expression might be associated with the remodeling of K^(+)cycling,degeneration of morphological structure and decrease of hearing function in aging C57 mice.In conclusion,we speculated that the reduction ofα2-Na/K-ATPase might play an important role in the pathogenesis of age-related hearing loss.

Establishment of Embryonic Stem Cell Lines from C57BL/6J Mice and Generation of Chimeras

Four embryonic stem (ES) cell lines, designated CE1, CE2, CE3 and CE4, were isolated from C57BL/6J blastocysts. The ratio of normal diploid composition of these cell lines is above 70%. To examine the differentiation potential of the ES cells, the CE2 cells were injected subcutaneously into syngenic mice, and many kinds of differentiated cells were observed on the sections of the teratoma derived from this ES cell line. On the other hand, to test the chimeric ability of the ES cells, the CE2 cells were microinjected into the blastocysts of ICR mice, and a chimera was obtained among living pups. These results show that CE2 ES cells are pluripotent stem cells, which can differentiate into many kinds of cell types, and can be used as a cell system for further research.

Behavioral study of an aging model induced by different doses of D-galactose in C57BL／6J mice

Rodents chronically injected with D-galactose (D-gal have been used as an animal model for brain aging or antiaging pharmacology research. However, the dose of D-gal used to induce behavioral impairment has been reported in a wide range. Besides, very few studies used the C57BL inbred mouse strain to construct this model, which is one of the

C57BL/6小鼠微小三毛滴虫的观察与种系发育分析

为鉴定从某外单位引进的C57BL/6小鼠粪样检测中发现的虫体种类,本试验对虫体进行形态学观察,并进行种系发育分析。取C57BL/6小鼠粪样涂片和瑞士染色后进行显微镜观察,提取粪样总DNA,根据三毛滴虫的16S rRNA进行PCR扩增后测序,运用MEGA11进行种系发育分析。显微镜观察发现,虫体符合微小三毛滴虫的形态学特征,测序发现微小三毛滴虫的基因序列与鼠三毛滴虫高度同源。

C57BL/6N小鼠早期视网膜变性及小胶质细胞活化状态研究

目的:观察C57BL/6N(Crb1 rd8/rd8)小鼠早期视网膜变性以及小胶质细胞的活化情况。方法:取雄性SPF级C57BL/6N小鼠15只、C57BL/6J小鼠15只,正常饲养。分别在入组时,入组4、8、12 wk,使用Micron-Ⅲ小动物视网膜影像系统进行双眼彩色眼底照相检查计算病变数量、病变面积。观察结束后处死小鼠,摘取右侧眼球制备视网膜组织切片,进行HE染色后光镜下观察视网膜组织形态;采用免疫组织化学染色分析两组小鼠视网膜CX3CR1的表达水平及位置。摘取左侧眼球分离视网膜,使用Western-Blot检测CD86、CD206的表达情况,使用电化学发光法测定视网膜中IL-1β、IL-6、TNF-α、IL-4、IL-10炎性因子含量水平。结果:眼底彩照结果显示在入组4、8、12 wk,C57BL/6N组眼底病变数量均较入组时显著增加,与C57BL/6J组同时间点变化量比较均有差异(均P<0.05);眼底病变面积变化量两组间入组12 wk有差异(P<0.05),各组内变化量比较均无差异(均P>0.05);视网膜组织HE染色示:C57BL/6N组视网膜结构异常,细胞排列疏松、紊乱,光感受器层向视网膜内侧明显的玻璃膜疣样凸起,C57BL/6J组视网膜结构清晰,细胞排列有序,无明显异常;免疫组化结果示:C57BL/6N组视网膜中CX3CR1高表达于神经节细胞层、内外丛状层、感光细胞层及病灶处位置,平均光密度为0.285±0.056,C57BL/6J组为0.189±0.084(P<0.05);Western-Blot结果显示:与C57BL/6J组相比,C57BL/6N组视网膜CD86、CD206蛋白有不同程度升高,两组CD86蛋白表达有差异(P<0.05);细胞因子检测结果显示:C57BL/6N组IL-1β、TNF-α水平显著高于C57BL/6J组,而IL-10含量则明显较低(均P<0.05)。结论:C57BL/6N(Crb1 rd8/rd8)小鼠视网膜变性进展缓慢,随年龄呈进行性加重,病灶部位视网膜结构紊乱,伴有以M1型极化为主的小胶质细胞浸润。

枸杞多糖对C57BL/6J骨质疏松症小鼠的早期防治

目的探讨枸杞多糖(LBP)对C57BL/6J骨质疏松症小鼠的早期防治作用。方法选用12周C57BL/6J雌鼠,采用双侧卵巢完全切除术制备骨质疏松症模型,假手术组切除卵巢周围脂肪组织,术后1周,选取手术成功的小鼠分为假手术组、模型组和LBP组。LBP组给予灌胃LBP 0.1 g·kg^(-1),1次/d,连续12周,假手术组和模型组灌胃等量蒸馏水。酶联免疫吸附(ELISA)法检测小鼠血清中雌二醇(E_(2))含量;生物化学方法检测血清氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)含量;Micro CT扫描胫骨微观结构,观察骨小梁厚度(Tb·Th)、骨表面积和骨体积之比(BS/BV)、骨小梁分离度(Tb·Sp)、骨密度(BMD)、骨体积分数(BV/TV)、骨体积(BV)的变化。结果与假手术组小鼠比较,模型组小鼠的体质量增长率升高、子宫脏器系数明显减小、血清E_(2)含量下降、血清氧化应激指标MDA含量增高、SOD活力降低(P均<0.01);与模型组小鼠比较,LBP组小鼠子宫脏器系数、血清E_(2)含量差异均无统计学意义(P均>0.05),但体质量增长率下降、血清MDA含量降低、SOD活力增加(P均<0.01),骨组织BMD、BV升高(P均<0.05),Tb·Th及BV/TV均显著升高(P均<0.01)。胫骨三维重建图显示,模型组骨皮质变薄,呈现骨质疏松改变,骨小梁数量减少,排列紊乱,髓腔明显扩大;LBP组较模型组骨小梁数量增加。结论LBP可能通过调控早期骨质疏松症小鼠的氧化应激反应,发挥改善骨质疏松的作用。

C57BL/6小鼠炎症性肠病模型的建立与指标评价

【目的】通过葡聚糖硫酸钠(dextran sulfate sodium,DSS)或脂多糖(lipopolysaccharide,LPS)刺激构建小鼠炎症性肠病(inflammatory bowel disease,IBD)模型,为IBD研究提供高效经济的模型参考,并为IBD抗炎药物筛选与药效评价提供可靠的方法支撑。【方法】本试验以C57BL/6雄性小鼠为研究对象,分别暴露于不同浓度的DSS(3%、4%和5%)7 d或LPS(5、10和20 mg/kg)4 h。通过测量小鼠结肠长度,HE染色观察肠道形态结构及实时荧光定量PCR检测肠道炎性因子的mRNA表达等对模型进行评价。【结果】DSS诱导小鼠结肠变短、病理出血、腺体萎缩、结构排列絮乱、炎性细胞浸润以及结肠中炎性因子白细胞介素-1β(interleukin-1β,IL-1β)、IL-6、IL-10和肿瘤坏死因子-α(tumour necrosis factor-α,TNF-α)的mRNA表达显著上升(P<0.05),4%DSS即可高效建立IBD模型。同时,LPS引起小鼠肠系膜出血、肠道肿胀和充血,肠绒毛高度降低,隐窝深度升高,小肠中IL-10和IL-1βmRNA水平升高、IL-6 mRNA水平下降,十二指肠和回肠TNF-αmRNA水平上升,空肠TNF-αmRNA水平降低,且以5 mg/kg LPS综合影响最为显著。【结论】4%DSS和5 mg/kg LPS是C57BL/6小鼠IBD模型建立的最适浓度,肠道病理形态改变、肠道炎性细胞浸润及炎性因子IL-1β、IL-6、IL-10和TNF-α的mRNA表达异常是评价IBD模型的重要指标。

C57BL/6J小鼠与昆明小鼠NIAAA法酒精性肝病造模效果的比较

为了对比C57BL/6J小鼠和昆明小鼠酒精性肝病模型建造效果及建模完成后续肝脏生化指标变化情况,为酒精性肝病的治疗研究提供基础依据,试验选择雌性C57BL/6J小鼠和昆明小鼠各36只,随机分为对照组(液体饲料+麦芽糊精)、建模16 d组与建模19 d组(液体饲料+酒精,n=12)。利用NIAAA法建造酒精性肝病小鼠模型,并分别于建模第16 d、建模第19 d取其血清及肝组织进行生化指标检测及病理切片观察。结果显示,C57BL/6J小鼠建模16 d组和建模19 d组与昆明小鼠建模16 d组的小鼠血清ALT、AST含量,肝脏MDA、TG含量均分别显著高于其对照组小鼠(P<0.05);而血清ALB含量、肝脏SOD活力显著低于其对照组小鼠(P<0.05)。C57BL/6J建模16 d组和建模19 d组小鼠的肝脏GSH含量和肝脏系数显著高于其对照组小鼠(P<0.05),但昆明小鼠建模19 d组除了肝脏SOD活力显著低于其对照组小鼠(P<0.05),其他指标均与其对照组小鼠无显著差异。病理切片结果表明,与对照组相比,建模16 d组2种小鼠肝细胞排列紊乱,肝细胞索断裂,肝细胞出现炎性浸润和脂肪液滴。C57BL/6J小鼠建模19 d组肝组织仍呈现病变,而昆明小鼠建模19 d组肝细胞已逐渐恢复正常排列。与昆明小鼠相比,C57BL/6J小鼠对于酒精的肝脏氧化应激损伤更严重,酒精性肝病模型更稳定。

孕期低剂量镉暴露对C57BL/6母鼠肾脏组织的毒性损伤作用

为研究孕期低剂量镉暴露对母鼠肾脏的毒性效应,基于前期构建的孕期低剂量镉暴露小鼠模型,通过组织显微切片技术对镉暴露母鼠的肾脏组织结构进行观察,并通过实时定量PCR法和蛋白质免疫印迹法对肾脏中炎症相关基因和凋亡调控基因的mRNA和蛋白质表达水平进行检测。实验结果显示:孕期低剂量镉暴露可导致肾脏结构出现一定程度的病变,如肾小球内细胞数目增多,肾小囊变窄,肾小球略微肿胀等;与对照组相比,镉暴露母鼠肾脏中凋亡功能调控基因Bax的mRNA和蛋白质表达水平显著升高。综上所述,孕期低剂量镉暴露可导致母体肾脏结构发生损伤,该毒性作用可能与凋亡调控基因Bax的表达水平升高相关。研究结果旨在阐明孕期低剂量镉暴露对母体器官的毒性效应机制,为镉暴露下家畜的母胎健康风险评估和环境管理提供基础数据支持。

顺铂诱导肾损伤模型在C57BL/6小鼠J和N亚型中的差异性研究

目的探讨C57BL/6 J和N亚型小鼠在顺铂诱导肾损伤模型中的易感性差异,为选择药物肾损伤模型提供理论依据。方法雄性C57BL/6小鼠J和N两种亚型,各随机分为正常组(Control)、模型组(cisplatin,CDDP)。模型组腹腔注射顺铂(3.33 mg·kg^(-1)),隔天给药,持续2周,记录小鼠体重变化、存活率;检测Scr、BUN和β2-MG评价肾功能;HE、Masson和PAS染色法观察肾皮质病理改变。IL-6、IL-1β和IL-18等炎症因子检测顺铂导致的肾脏炎症反应。结果与Control组相比,CDDP组两种亚系小鼠Scr、BUN和β2-MG均明显升高;组织学结果显示肾小管损伤,胶原纤维化和糖原沉积均增加;IL-6、IL-1β和IL-18炎症因子含量和肾皮质蛋白表达均明显升高。C57BL/6N与C57BL/6J系小鼠比较,C57BL/6N系小鼠肾功能指标Scr和BUN均值明显偏高(P<0.01),组织学评分各病理损伤较C57BL/6J亚系小鼠明显偏高,反映纤维化的Masson染色差异具有统计学意义(P<0.01);IL-6、IL-1β和IL-18均值明显偏高,但差异无统计学意义。结论C57BL/6 J和N亚型小鼠均可使用顺铂诱导成功肾损伤模型,相比于C57BL/6J小鼠,C57BL/6N亚系小鼠在肾功能、组织学以及炎症因子等方面损伤明显,提示C57BL/6N亚系小鼠更适于作为药物或者其他肾功能损伤模型的建立。