Improved Protein Transfer Efficiency and Signal Intensity in BlotMan Using Pulse Width Modulation

BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.

Lumped Parameter Mass Flow Rate and Pressure Control Characteristics Model for High-Speed Pneumatic PWM on/off Valve

Aiming at solving the problem of strong coupling characteristic of the key parameters of high-speed pneumatic pulse width modulation( PWM) on / off valve, a general lumped parameter mathematical model based on the valves time periods was well developed. With this model,the mass flow rate and dynamic pressure characteristics of constant volumes controlled by high-speed pneumatic PWM on /off valves was well described. A variable flow rate coefficient model was proposed to substitute for the constant one used in most of the prior works to investigate PWM on /off valves' dynamical pressure response, and a formula for disclosing the inherent relationship among the PWM command signal,static mass flow rate,and sonic conductance of the valve was newly derived.Finally,an extensive set of analytical experimental comparisons were implemented to verify the validity of the proposed mathematica model. With the proposed model, PWM on /off valves' characteristics,such as mass flow rate,step pressure response of the valve control system,mean pressure and ripple amplitude,not only in the linear range,but also in the nonlinear range can be wel predicted; Good agreement between measured and calculated results was obtained,which proved that the model is helpful for designing a control strategy in a closed loop control system.

New Approach of Multi-Cell Stacked Cell Inverter for Solar Photovoltaic System

In this paper, a new inverter topology dedicated to isolated or grid-connected PV systems is proposed. This inverter is based on the structures of a stacked multi-cell converter (SMC) and an H-bridge. This new topology has allowed the voltage stresses of the converter to be distributed among several switching cells. Secondly, divide the input voltage into several fractions to reduce the number of power semiconductors to be switched. In this contribution, the general topology of this micro-inverter has been described and the simulation tests developed to validate its operation have been presented. Finally, we discussed the simulation results, the efficiency of this topology and the feasibility of its use in a grid-connected photovoltaic production system.

Optimized Design and Analysis of Single-Phase and Three-Phase Inverters for Efficient Power Conversion: A Comparative Study

A large amount of switching loss occurs in the inverter. From this point of view, an inverter design should be optimized for which size and cost will be minimum along with increasing efficiency. The main aim of this paper is the analysis and development of single-phase and three-phase inverter to design with MOSFET and IGBT as power elements by sinusoidal pulse width modulation (SPWM) technique using MATLAB Simulink software and compare their difference with the practical inverter. This work proposes different multilevel stages for the cascaded H-Bridge inverter to enhance the output voltages. Then compare their performance, harmonic distortion, and frequency spectrum. The hardware of an inverter circuit has been developed using the SG3524 microcontroller. The main goal of this design is to generate a sine wave with fewer harmonics, while keeping the cost and complexity of the circuit low. The designed inverter has undergone testing with different AC loads and is primarily intended for low-power applications, as lamps, fans, and chargers. This design aims to provide a reliable and efficient inverter solution for these specific applications.

小型全电艇动力总成系统

为了提高小型船艇的操作性能和环保性能,采用数字舵机和直流无刷电机设计一种小型吊舱式推进器,并在此基础上以锂电池组作为动力能源,结合DSP(digital signal processing)技术,开发相应的推进控制系统。样船测试结果表明:该小型全电艇动力总成系统能够满足船速、转向、续航能力等方面的性能要求,运行稳定。

Design of vision-based soccer robot using DSP

A new design of vision based soccer robot using the type TMS320F240 of DSPs for MiroSot series is presented. The DSP used enables cost effective control of DC motor, and features fewer external components, lower system cost and better performances than traditional microcontroller. The hardware architecture of robot is firstly presented in detail, and then the software design is briefly discussed. The control structure of decision making subsystem is illuminated also in this paper. The conclusion and prospect are given at last.

Integration and verification case of IP-core based system on chip design

In this paper, the design and verification process of an automobile-engine-fan control system on chip （SoC） are introduced. The SoC system, SHU-MV08, reuses four new intellectual property （IP） cores and the design flow is accomplished with 0.35 btm chartered CMOS technology. Some special functions of IP cores, the detailed integration scheme of four IP cores, and the verification method of the entire SoC are presented. To settle the verification problems brought by analog IP cores, NanoSim based chip-level mixed-signal verification method is introduced. The verification time is greatly reduced and the first tape-out achieves success which proves the validity of our design.

A Review of Drive Techniques for Multiphase Machines

Multiphase machines are gaining increasing popularity because of their distinct advantages over three-phase counterparts,such as reduced per phase power rating,improved reliability and increased degrees of freedom.This paper overviews the development history of multiphase machines and drive techniques in Section II.Three kinds of drive algorithms,including Field-Oriented Control(FOC),Direct Torque Control(DTC)and Model Predictive Control(MPC),are introduced in Section III.Then two types of fault-tolerant methods are compared in Section IV,which are the Vector Space Decomposition(VSD)strategy and Optimal Current Control(OCC)method.Furthermore,different types of existing multiphase converter topologies are listed in Section V,and various kinds of multiphase PWM methods are discussed in Section VI.Finally,based on the review,developing trends of multiphase drive techniques are forecasted.

Torque Sharing Function Control of Switched Reluctance Machines with Reduced Current Sensors

This paper presents a Torque Sharing Function(TSF)control of Switched Reluctance Machines(SRMs)with different current sensor placements to reconstruct the phase currents.TSF requires precise phase current information to ensure accurate torque control.Two proposed methods with different chopping transistors or a new PWM implementation require four or two current sensors to replace the current sensors on each phase regardless of the phase number.For both approaches,the actual phase current can be easily extracted during the single phase conducting region.However,how to separate the incoming and outgoing phase current values during the commutation region is the difficult issue to deal with.In order to derive these two adjacent currents,the explanations and comparisons of two proposed methods are described.Their effectiveness is verified by experimental results on a four-phase 8/6 SRM.Finally,the approach with a new PWM implementation is selected,which requires only two current sensors for reducing the number of sensors.The control system can be more compact and cheaper.

A New Switching Scheme for Z-source Inverter to Minimize Ripples in the Z-source Elements

This paper presents a modification in pulse width modulation （PWM） scheme with unequal shoot-through distribution for the Z-source inverter （ZSI） which can minimize ripples in the current through the Z-source inductors as well as the voltage across the Z-source capacitors. For the same system parameters, the proposed control technique provides better voltage boost across the Z-source capacitor, DC-link, and also the AC output voltage than the traditional PWM. The ripples in the Z-network elements are found to be reduced by 75 % in the proposed modulation scheme with optimum harmonic profile in the AC output. Since the Zqnetwork requirement will be based on the ripple profile of the elements, the Z-network requirements can be greatly reduced. The effectiveness of the proposed modulation scheme has been simulated in Matlab/Simulink software and the results are validated by the experiment in the laboratory.

Design and Analysis of Cascaded Hybrid-Bridge Multi-Cell Multilevel Inverter with Reduced Total Harmonic Distortion Profile

This multilevel inverter methodology is the center of focus among researchers in recent era.It has been focused due to its advantages over existing topologies,drawbacks and improvement of power quality,Multi-level inverter has the ability to generate nearly sinusoidal waves.This sinusoidal wave can be further improved by increasing the level of output voltage or with the help of filter design,and this manuscript presents single-phase Multi cell Multi-Level Inverter(MLI).It has been considered for reducing component count to get a higher number of output voltage levels and lower Total harmonics distortion profile.It comprises with four symmetric DC input voltage and 10 IGBT switches to produces stepped output of 9 level,and when deploy asymmetric Dc voltage source the same circuit will produce 31 level output with some changes in firing scheme,moreover this circuit is the family of cascaded hybrid bridge inverter so this circuit covered advantage of CHB MLI,This circuit uses lower no.of switch as compared to existing conventional MLIs such as FC-MLI,CHB-MLI,NPC-MLI,This paper also provides one of most pertinent controls and modulation mechanisms for a MLI using a hybrid reference/carrier-oriented sinusoidal PWM mechanism.At last,simulated outcomes are to validate the performance of both architectures in MLI structure as well as verify the concept.

A Novel Multilevel Inverter Employing Additive and Subtractive Topology

As the demand for high voltage, high power inverters are increasing and there is a problem of connecting a power semiconductor switch directly to a high voltage network. As a part of this the multilevel inverters had been introduced. As a part of this, several researches had been done for the development of multilevel inverters. The commercially available and extensively studied topologies for multilevel voltage output are Neutral Point Clamped (NPC), Cascaded Half Bridge (CHB) and Flying Capacitor (FC) converters. However, with these existing topologies, there is a significant increase in the number of power switches and passive components. Thus it leads to more complex control circuitry and overall cost of the system increase with increase in the output levels. In this paper, a novel multilevel inverter is proposed in which it employs additive and subtractive topology to get higher output levels. This approach significantly reduces the number of power switches needed as compared to existing topology. The present developed multilevel inverter can generate only five voltage levels. With this proposed topology the multilevel inverter can be modified to nine-level inverter. Moreover modified hybrid multicarrier Pulse Width Modulation (PWM) technique can be implemented in the proposed multilevel inverter in order to obtain uniform switch utilization and lower THD. An appropriate modulation scheme is presented and also the proposed concept is analyzed through simulation studies.

State Space Modeling and Implementation of a New Transformer Based Multilevel Inverter Topology with Reduced Switch Count

This paper presents a new transformer based multilevel inverter, with a novel pulse width modulation scheme to achieve seven-level inverter output voltage. The proposed inverter switching pattern consists of three fundamental frequency sinusoidal reference signals with an offset value, and one high frequency triangular carrier signal. This switching scheme has been implemented using an 8-bit Xilinx SPARTAN-3E field programmable gate array based controller. In addition, the state space model of the proposed inverter is developed. The significant features of the proposed topology are: reduction of the power switch count and the gate drive power supply unit, the provision of a galvanic isolation between load and sources by a centre tap transformer. An exhaustive comparison has been made of the existing multilevel inverter topologies and the proposed topology. The performances of the proposed topology with resistive, resistive-inductive loads are simulated in a MATLAB environment and validated experimentally on a laboratory prototype.

Enhancement of Solar PV Panel Efficiency Using Double Integral Sliding Mode MPPT Control

The extraction of maximum power from the solar panels,using the sliding mode control scheme,becomes popular for partial weather atmospheric conditions due to its effective dynamic duty cycle ratio.However,the sliding mode control scheme was sophisticated with single integral and double integral sliding mode control scheme,which offer enhanced maximum power extraction and support enhanced solar panel efficiency in partial weather conditions.The operation of the sliding mode control scheme depends on the selection of a sliding surface selection based on the atmospheric weather condition,which enables the effective sliding duty cycle ratio operation for the DC/DC boost converter.The duty cycle ratio of the sliding mode control resembles the usual dynamic behavior to achieve enhanced efficiency compared to the various maximum power point tracking(MPPT)schemes.The major limitation of the sliding mode control scheme is to achieve the steady state voltage error of the solar panel in minimum settling time duration.The single integral sliding mode control scheme achieves the expected steady state voltage error limit but fails to achieve minimum settling time duration.Hence,the single integral sliding mode control is extended to a double integral sliding mode control scheme to achieve both steady state voltage error limits within the minimum settling time duration.This double integral sliding mode control scheme allows us to obtain the higher sliding surface duty cycle ratio which acts as the input signal to the boost converter.This activates the enhanced stable and reliable system operation,and nullifies the lacuna of maximum solar panel efficiency under partial weather conditions.Hence,this paper aims to present the design and performance operation of the double integral sliding mode(DISM)MPPT control scheme.To validate the performance analysis of the proposed DISM MPPT control scheme,the MATLAB/Simulink model is designed and verified.Also,the performance analysis of the proposed DISM MPPT control scheme is compared with the sliding mode controller(SMC)scheme and single integral sliding mode controller(SiSMC)scheme.The performance analysis of the proposed double integral sliding mode controller(DISMC)scheme attains 99.10%of efficiency and a very less setting time of 0.035s when compared to other existingmethods.

Droplet size and spray pattern characteristics of PWM-based continuously variable spray

A Pulse-Width-Modulation-based(PWM-based)continuously variable sprayer was developed using a proportional regulating solenoid valve.Variable flow-rate was obtained by varying the duty cycle of the actuating signal with 24 kHz frequency.Flow-rate regulating ranges of the PWM-based continuously variable spray(i.e.the turndown ratio responding to 100%-40%duty cycle)are 7.14:1,3.57:1,and 3.70:1 for flat-fan,hollow-cone and solid-cone nozzles,respectively.The purpose of the study was to evaluate the PWM-based continuously variable spray.The method was to quantify the effects of flow-rate control on spray characteristics in terms of droplet size spectra,spray distribution patterns,and spray angle for flat-fan,hollow-cone,solid-cone nozzles.For all nozzles tested,spray distribution concentrated on the center of the spray field with the decrease of flow-rate.But the spray shape is still symmetrical.The sensitivities of the spray angles to flow-rate were 0.83,0.67,and 0.58(o)/%respectively for flat-fan,hollow-cone and solid-cone nozzles.Compared with the sensitivities of spray angle for PWM-based intermittent variable spray,they are somewhat larger.As flow-rate was reduced from the maximum(100%flow-rate)to the minimum controllable rate,the observed median diameter of spray droplets decreased by 5.4%,9.8%,and 9.9%for flat-fan,hollow-cone and solid-cone nozzles,respectively.This indicates that spray droplet size was affected slightly by flow-rate control.

DFIG sliding mode control fed by back-to-back converter with DC-link voltage control for variable wind turbine PWM speed

This paper proposes an indirect power control of doubly fed induction generator （DFIG） with the rotor connected to the electric grid through a back-to-back pulse width modulation （PWM） converter for variable speed wind power generation. Appropriate state space model of the DFIG is deduced. An original control strategy based on a variable structure control theory, also called sliding mode control, is applied to achieve the control of the active and reactive power exchanged between the stator of the DFIG and the grid. A proportional-integral-（P1） controller is used to keep the DC-link voltage constant for a back-to-back PWM converter. Simulations are conducted for validation of the digital controller operation using Matlab/Simulink software.

Spatial distribution visualization of PWM continuous variable-rate spray

Pesticide application is a dynamic spatial distribution process,in which spray liquid should be able to cover the targets with desired thickness and uniformity.Therefore,it is important to study the 2-D and 3-D(dimensional)spray distribution to evaluate spraying quality.The curve-surface generation methods in Excel were used to establish 1-D,2-D,and 3-D graphics of variable-rate spray distribution in order to characterize the space distribution of the variable-rate spray.The 1-D,2-D,and 3-D distribution graphs of Pulse-Width Modulation(PWM)-based continuous variable-rate spray were developed to provide a tool to analyze the distribution characteristics of the spray.The 1-D graph showed that the spray distribution concentrated toward the center of the spray field with the decreased flow-rate.The 2-D graph showed that the spray distribution always spread as the shape of Normal Probability Distribution with the change of the flow-rate.The 3-D graph showed that the spray distribution tended to be uniform when the sprayer travelled forward at the appropriate speed.This study indicated that the visualization method could be directly used for analysis and comparison of different variable-rate spray distributions from different experimental conditions and measuring methods.

Wide Symmetrical Dynamic Range PWM Neuron Circuit with Power Efficient Architecture

A novel pulse stream neuron circuit is presented whose output pulse width facilitates sigmoid activation to activate the function of neurons. The wide symmetrical dynamic range of this neuron ensures high noise immunity. The pulsed activation strategy provides a power efficient architecture, so the circuit has very low power dissipation. The simplicity of the circuit ensures its suitability for large-scale integration.

Reduction of High-frequency Vibration Noise for Dual-branch Three-phase Permanent Magnet Synchronous Motors

Two-level voltage source inverters with pulse width modulation(PWM)generate multiple carrier harmonics,thereby exciting high-frequency noise from the motor.Based on a special dual-branch three-phase permanent magnet synchronous motor(PMSM),a method that utilizes a known modified space vector PWM technique is proposed.This method is able to suppress unpleasant high-frequency vibration noise as well as acoustic noise more effectively than other methods.Vibration noise near the twice carrier frequency is eliminated,and PWM vibration noise around the carrier frequency is also reduced.In particular,vibration and acoustic noise at the twice carrier frequency are eliminated using a carrier phase-shift of 0.5πand the special winding structure of the dual-branch PMSM.The effectiveness of the proposed method is confirmed using detailed experimental results.

A low-power time-domain VCO-based ADC in 65 nm CMOS

A low-power, high-FoM （figure of merit）, time-domain VCO （voltage controlled oscillator）-based ADC （analog-to-digital converter） in 65 nm CMOS technology is proposed. An asynchronous sigma-delta modulator （ASDM） is used to convert the voltage input signal to a square wave time signal, where the information is contained in its pulse-width. A time-domain quantizer, which uses VCO to convert voltage to frequency, is adopted, while the XOR （exclusive-OR） gate circuits convert the frequency information to digital representatives. The ASDM does not need an external clock, so there is no quantization noise. At the same time, the ASDM applies a harmonic- distortion-cancellation technique to its transconductance stage, which increases the SNDR （signal to noise and distortion ratio） performance of the ASDM. Since the output of the ASDM is a two-level voltage signal, the VCO＇s V-F （voltage to frequency） conversion curve is always linear. The XOR phase quantizer has an inherent feature of first-order noise-shaping. It puts the ADC＇s low-frequency output noise to high-frequency which is further filtered out by a low-pass filter. The proposed ADC achieves an SNR/SNDR of 54. dB/54.3 dB in the 8 MHz bandwidth, while consuming 2.8 mW. The FoM of the proposed ADC is a 334 fJ/conv-step.