目的探讨应用D-半乳糖(D-gal)建立小鼠肝脏损伤模型的方法.方法将20只小鼠随机分为两组,正常对照组应用生理盐水20 m L/(κg·d-1)皮下注射,模型组应用D-半乳糖按500 mg/(κg·d-1)皮下注射.造模完成后采用比色法测定血清AST,AL...目的探讨应用D-半乳糖(D-gal)建立小鼠肝脏损伤模型的方法.方法将20只小鼠随机分为两组,正常对照组应用生理盐水20 m L/(κg·d-1)皮下注射,模型组应用D-半乳糖按500 mg/(κg·d-1)皮下注射.造模完成后采用比色法测定血清AST,ALT及GST;放免法测定血浆PGE2,TNF及IL-6;Western blot方法检测肝组织COX-2表达.通过光镜观察肝组织病理变化.结果血浆ALT,AST及GST模型组与正常对照组比较有明显升高(P<0.01);模型组与正常对照组比较血浆PGE2,TNF及IL-6升高明显,差异具有统计学意义(P<0.01);病理切片显示肝脏结构呈现病理改变.结论应用D-半乳糖可以成功制作小鼠肝脏损伤模型.展开更多
Objective： Cheongseoikki-tang （CIT, Korean）, also called Qingshu Yiqi decoction（清署益气汤）and Seisho-ekki-to （Japanese）, is well known as an effective traditional combination of herbs for treating cardiovascul...Objective： Cheongseoikki-tang （CIT, Korean）, also called Qingshu Yiqi decoction（清署益气汤）and Seisho-ekki-to （Japanese）, is well known as an effective traditional combination of herbs for treating cardiovascular diseases. This study was to research its effects on bone marrow-derived mast cell （BMMC）-mediated allergy and inflammation mechanisms. Methods： In this study, the biological effect of Cheongseoikki-tang ethanol extract （CITE） was evaluated, focusing on its effects on the production of allergic mediators by phorbol 12-myfistate 13-acetate （PMA） plus calcium ionophore A23187 （A23187）-stimulated BMMCs. These allergic mediators included intedeukin-6 （IL-6）, prostaglandin D2 （PGD2）, leukotfiene （34 （LTC4）, and β-hexosaminidase （13-hex）. Results： Our data revealed that CITE inhibited the production of IL-6, PGD2,/TC4, and 15-hex induced by PMA plus A23187 （P〈0.05）. Conclusion： These findings indicate that CITE has the potential for use in the treatment of allergy.展开更多
文摘目的探讨应用D-半乳糖(D-gal)建立小鼠肝脏损伤模型的方法.方法将20只小鼠随机分为两组,正常对照组应用生理盐水20 m L/(κg·d-1)皮下注射,模型组应用D-半乳糖按500 mg/(κg·d-1)皮下注射.造模完成后采用比色法测定血清AST,ALT及GST;放免法测定血浆PGE2,TNF及IL-6;Western blot方法检测肝组织COX-2表达.通过光镜观察肝组织病理变化.结果血浆ALT,AST及GST模型组与正常对照组比较有明显升高(P<0.01);模型组与正常对照组比较血浆PGE2,TNF及IL-6升高明显,差异具有统计学意义(P<0.01);病理切片显示肝脏结构呈现病理改变.结论应用D-半乳糖可以成功制作小鼠肝脏损伤模型.
基金Supported by the National Research Foundation of Korea Grant (No.NRF-2010-013-E00034)the Grant of the Traditional Korean Medicine R&D project,Ministry of Health & Welfare, Republic of Korea(No.B120069)
文摘Objective： Cheongseoikki-tang （CIT, Korean）, also called Qingshu Yiqi decoction（清署益气汤）and Seisho-ekki-to （Japanese）, is well known as an effective traditional combination of herbs for treating cardiovascular diseases. This study was to research its effects on bone marrow-derived mast cell （BMMC）-mediated allergy and inflammation mechanisms. Methods： In this study, the biological effect of Cheongseoikki-tang ethanol extract （CITE） was evaluated, focusing on its effects on the production of allergic mediators by phorbol 12-myfistate 13-acetate （PMA） plus calcium ionophore A23187 （A23187）-stimulated BMMCs. These allergic mediators included intedeukin-6 （IL-6）, prostaglandin D2 （PGD2）, leukotfiene （34 （LTC4）, and β-hexosaminidase （13-hex）. Results： Our data revealed that CITE inhibited the production of IL-6, PGD2,/TC4, and 15-hex induced by PMA plus A23187 （P〈0.05）. Conclusion： These findings indicate that CITE has the potential for use in the treatment of allergy.
