Objective： To study the effect of the extract of Prunella vulgaris L. on multiple drugs resistant bacillus tuberculosis （MDR-TB）. Methods： Experimental animal model in rats was induced by MDR-TB. Normal group mode...Objective： To study the effect of the extract of Prunella vulgaris L. on multiple drugs resistant bacillus tuberculosis （MDR-TB）. Methods： Experimental animal model in rats was induced by MDR-TB. Normal group model group and Prunella vulgaris L. group were set up. The contents of IFN-7, IL-4, IL-10 and IL-12 were examined by ELISA. Their genome mRNAs were extracted, the target genes were amplified by PCR. RT-PCR was used to detect the mRNA levels of them. Results： The content of IFN-q, of the extract of Prunella vulgaris L. group was 1.98±0.67 pg/ml, IL-4 was 6.47±1.46 pg/ml, IL-10 was 12.13±3.43 pg/ml and IL-12 was 3.02±0.86 pg/ml. Compared with the model group, Prunella vulgaris L. group was notable difference in serum IFN-γ, IL-12 and IL-10 （P〈0.05）. The mRNA levels of IFN-γ, IL-12 increased and IL-10 decreased obviously, the differences were quite significant （P〈0.05）, but IL-4 had no obvious change. Conclusion： The extract of Prunella vulgaris L. can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription, accordingly provide the theory basis of healing of tuberculosis with it.展开更多
Objective:The aim of this study was to observe the effect of the Prunella vulgaris L extract on the Jurkat human T lymphoma cell line.Methods:Jurkat cells were cultivated with different concentrations of the extract f...Objective:The aim of this study was to observe the effect of the Prunella vulgaris L extract on the Jurkat human T lymphoma cell line.Methods:Jurkat cells were cultivated with different concentrations of the extract from Prunella vulgaris L.The MTT assay and flow cytometry were employed to determine the cells' proliferation inhibition ratio and the apoptosis rates,respectively.Agarose gel electrophoresis was used to observe cellular DNA fragmentation,and western blotting was used to observe changes in Bcl-2 and Bax protein expression.Results:The Prunella vulgaris L extract remarkably inhibited the proliferation of Jurkat cells.This inhibition exhibited dose dependence,with an IC50 of 20.23 ± 0.31 μg/mL.Agarose gel electrophoresis showed that the apoptosis strap became wider and brighter,and flow cytometry showed that the apoptosis rate increased in a concentration-dependent manner.Western blotting showed that Bcl-2 protein was down-regulated and Bax protein was up-regulated during apoptosis.Conclusion:The extract from Prunella vulgaris L induced apoptosis of Jurkat cells by down-regulating Bcl-2 protein and up-regulating Bax protein.These actions inhibited the growth of Jurkat cells.展开更多
Objective Pelvic inflammatory disease(PID)is one of the most common gynaecological diseases.Here,this thesis aims to investigate the therapeutic effects of Prunella vulgaris L.oil on the PID by using metabolomics base...Objective Pelvic inflammatory disease(PID)is one of the most common gynaecological diseases.Here,this thesis aims to investigate the therapeutic effects of Prunella vulgaris L.oil on the PID by using metabolomics based on gas chromatographymass spectrometry(GC-MS)to address this challenge.Methods First,measurements of pro-inflammatory cytokines and histological analysis of the uterus were conducted to validate the successful generation of a PID rat model.Furthermore,the volatile oil from Prunella vulgaris L.was administered to treat PID rats.Serum samples were collected before and after treatment and analyzed by GC-MS to generate metabolite profiles for each sample.The information generated from the qualitative and quantitative analysis of these metabolites was applied to distinguish between the PID model and normal control groups.Results Some metabolites,such as acetic acid,succinic acid,glyceric acid,(R*,S*)-3,4-dihydroxybutanoic acid,3-hydroxyphenylacetic acid,D-ribose and myo-inositol showed a higher contribution in the classification model;thus,they can be considered as potential biomarkers.Furthermore,the therapeutic effect of the volatile oil extracted from Prunella vulgaris L.could also be visualized using GC-MS-based metabolomics.Conclusions The results show that metabolomics studies are invaluable for disease diagnosis and therapeutic effect estimation.展开更多
Prunella vulgaris,a traditional Chinese medicine(TCM),exerts a significant hypotensive effect,particularly in managing various forms of hypertension.Nonetheless,the precise antihypertensive constituents and their resp...Prunella vulgaris,a traditional Chinese medicine(TCM),exerts a significant hypotensive effect,particularly in managing various forms of hypertension.Nonetheless,the precise antihypertensive constituents and their respective targets remain elusive.This study endeavored to identify the hypotensive components of P.vulgaris and elucidated its mode of action based on hypotensive targets.Utilizing the Systematic Pharmacology of Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),UniProt,GeneCards,STRING,RCSB,and the Human Genome Annotation and Analysis Database(Metascape),we conducted a screening of active ingredients and hypotensive targets.The Network Analyzer software Cytoscape 3.9.0 facilitated a comprehensive analysis of the active ingredients.Molecular docking was executed employing Sybyl-X 2.0 and Discovery Studio 2019.Predictions and analyses of the pharmacokinetics and toxicity of active ingredients were performed using the ADMETlab 2.0 online platform.Eight active compounds(1–8)and 11 hypotensive targets were identified,with IL1B and PPARG exhibiting a high degree of correlation.Dominant biological processes included negative regulation of apoptotic processes,positive regulation of gene expression,response to xenobiotic stimulus,and response to hypoxia.KEGG analysis unveiled core pharmacological mechanisms,notably fluid shear stress and atherosclerosis,lipid and atherosclerosis,P13K-Akt,MAPK,and relaxin signaling pathways.Compounds 5–8 demonstrated robust interactions with multiple targets through hydrogen bonds,van der Waals forces,and pi-alkyl interactions,which serve as primary stabilizers of docking complexes.Notably,compound 7 exhibited promising ADMET prediction results,suggesting its potential for drug molecule development.Our findings underscored the synergistic effects of P.vulgaris on multiple targets and pathways in hypertension treatment,reflecting the characteristic multi-component and multi-target effects of TCM.展开更多
基金Supported by the Natural Science Foundation for Universities in Anhui Province (KJ2010A087 and KJ2008A152)
文摘Objective： To study the effect of the extract of Prunella vulgaris L. on multiple drugs resistant bacillus tuberculosis （MDR-TB）. Methods： Experimental animal model in rats was induced by MDR-TB. Normal group model group and Prunella vulgaris L. group were set up. The contents of IFN-7, IL-4, IL-10 and IL-12 were examined by ELISA. Their genome mRNAs were extracted, the target genes were amplified by PCR. RT-PCR was used to detect the mRNA levels of them. Results： The content of IFN-q, of the extract of Prunella vulgaris L. group was 1.98±0.67 pg/ml, IL-4 was 6.47±1.46 pg/ml, IL-10 was 12.13±3.43 pg/ml and IL-12 was 3.02±0.86 pg/ml. Compared with the model group, Prunella vulgaris L. group was notable difference in serum IFN-γ, IL-12 and IL-10 （P〈0.05）. The mRNA levels of IFN-γ, IL-12 increased and IL-10 decreased obviously, the differences were quite significant （P〈0.05）, but IL-4 had no obvious change. Conclusion： The extract of Prunella vulgaris L. can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription, accordingly provide the theory basis of healing of tuberculosis with it.
文摘Objective:The aim of this study was to observe the effect of the Prunella vulgaris L extract on the Jurkat human T lymphoma cell line.Methods:Jurkat cells were cultivated with different concentrations of the extract from Prunella vulgaris L.The MTT assay and flow cytometry were employed to determine the cells' proliferation inhibition ratio and the apoptosis rates,respectively.Agarose gel electrophoresis was used to observe cellular DNA fragmentation,and western blotting was used to observe changes in Bcl-2 and Bax protein expression.Results:The Prunella vulgaris L extract remarkably inhibited the proliferation of Jurkat cells.This inhibition exhibited dose dependence,with an IC50 of 20.23 ± 0.31 μg/mL.Agarose gel electrophoresis showed that the apoptosis strap became wider and brighter,and flow cytometry showed that the apoptosis rate increased in a concentration-dependent manner.Western blotting showed that Bcl-2 protein was down-regulated and Bax protein was up-regulated during apoptosis.Conclusion:The extract from Prunella vulgaris L induced apoptosis of Jurkat cells by down-regulating Bcl-2 protein and up-regulating Bax protein.These actions inhibited the growth of Jurkat cells.
基金We thank for the funding support from the National Natural Science Foundation of China(No.81503041)Natural Science Foundation of Hunan Province(No.2017JJ4045)Changsha Science and Technology Project(No.kq1701073).
文摘Objective Pelvic inflammatory disease(PID)is one of the most common gynaecological diseases.Here,this thesis aims to investigate the therapeutic effects of Prunella vulgaris L.oil on the PID by using metabolomics based on gas chromatographymass spectrometry(GC-MS)to address this challenge.Methods First,measurements of pro-inflammatory cytokines and histological analysis of the uterus were conducted to validate the successful generation of a PID rat model.Furthermore,the volatile oil from Prunella vulgaris L.was administered to treat PID rats.Serum samples were collected before and after treatment and analyzed by GC-MS to generate metabolite profiles for each sample.The information generated from the qualitative and quantitative analysis of these metabolites was applied to distinguish between the PID model and normal control groups.Results Some metabolites,such as acetic acid,succinic acid,glyceric acid,(R*,S*)-3,4-dihydroxybutanoic acid,3-hydroxyphenylacetic acid,D-ribose and myo-inositol showed a higher contribution in the classification model;thus,they can be considered as potential biomarkers.Furthermore,the therapeutic effect of the volatile oil extracted from Prunella vulgaris L.could also be visualized using GC-MS-based metabolomics.Conclusions The results show that metabolomics studies are invaluable for disease diagnosis and therapeutic effect estimation.
基金supported by the Beijing Municipal Education Commission for the laboratory construction(01422600012013-001549)talent training mode innovation test site(01422600012013-001538)
基金National Natural Science Foundation of China(Grant No.21702178)Training Plan of Young Backbone Teachers in Universities of Henan Province(Grant No.2021GGJS144)+5 种基金Key Scientific Research Program in Universities of Henan Province(Grant No.22A350009 and 23A350012)National Undergraduate Training Program for Innovation and Entrepreneurship(Grant No.202410480007 and 202310480020)Undergraduate Training Program for Innovation and Entrepreneurship of Henan Province(Grant No.202210480020 and 202210480021)Scientific Research Innovation Team of Xuchang University(Grant No.2022CXTD007)Horizontal Cooperation Project(Grant No.2023HX181)Undergraduate Training Program for Innovation and Entrepreneurship of Xuchang University(Grant No.X202210480016)。
文摘Prunella vulgaris,a traditional Chinese medicine(TCM),exerts a significant hypotensive effect,particularly in managing various forms of hypertension.Nonetheless,the precise antihypertensive constituents and their respective targets remain elusive.This study endeavored to identify the hypotensive components of P.vulgaris and elucidated its mode of action based on hypotensive targets.Utilizing the Systematic Pharmacology of Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),UniProt,GeneCards,STRING,RCSB,and the Human Genome Annotation and Analysis Database(Metascape),we conducted a screening of active ingredients and hypotensive targets.The Network Analyzer software Cytoscape 3.9.0 facilitated a comprehensive analysis of the active ingredients.Molecular docking was executed employing Sybyl-X 2.0 and Discovery Studio 2019.Predictions and analyses of the pharmacokinetics and toxicity of active ingredients were performed using the ADMETlab 2.0 online platform.Eight active compounds(1–8)and 11 hypotensive targets were identified,with IL1B and PPARG exhibiting a high degree of correlation.Dominant biological processes included negative regulation of apoptotic processes,positive regulation of gene expression,response to xenobiotic stimulus,and response to hypoxia.KEGG analysis unveiled core pharmacological mechanisms,notably fluid shear stress and atherosclerosis,lipid and atherosclerosis,P13K-Akt,MAPK,and relaxin signaling pathways.Compounds 5–8 demonstrated robust interactions with multiple targets through hydrogen bonds,van der Waals forces,and pi-alkyl interactions,which serve as primary stabilizers of docking complexes.Notably,compound 7 exhibited promising ADMET prediction results,suggesting its potential for drug molecule development.Our findings underscored the synergistic effects of P.vulgaris on multiple targets and pathways in hypertension treatment,reflecting the characteristic multi-component and multi-target effects of TCM.
