基于UHPLC-Q-Exactive Orbitrap MS技术的血藤果化学成分分析

目的建立分析血藤果化学成分的超高效液相色谱串联四极杆轨道阱质谱(UHPLC-Q-Exactive Orbitrap MS)法。方法色谱柱为Thermo Scientific Hypersil GOLD^(TM)aQ柱(100mm×2.1mm,1.9μm),流动相为乙腈(含0.1%甲酸)-0.1%甲酸溶液(梯度洗脱),流速为0.3mL/min,柱温为40℃,进样量为1μL;离子源为电喷雾(ESI)离子源,分别采用正、负离子扫描模式。结果采用一级母离子全扫描和数据依赖性前三强二级子离子扫描模式,通过数据库、对照品及参考文献共鉴定出51个化学成分,包括丝氨酸、L-精氨酸、天冬酰胺、谷氨酸等氨基酸类成分15个,柠檬酸、酒石酸、奎宁酸、莽草酸等有机酸类成分21个,生物碱、核苷等其他类成分15个。结论所建立的方法可高效、快捷地鉴定血藤果的化学成分,为后续药效物质和质量标准研究奠定了一定基础。

美国栎属种源引种、变异研究:种子及苗期生长变异

对从美国３个州（宾夕法尼亚、纽约和田纳西州）引进的栎属３个树种北方红栎、柳叶栎和红栎的２１个种源进行了两次播种和容器育苗试验。初步掌握了栎属种子砂床播种、芽苗移栽、容器育苗的关键技术。对栎属种子的发芽、芽苗和苗木的生长过程等有了一定的了解。发现栎属不同树种间和种内种源、家系间在种子体积、千粒重、发芽率、３０ｄ萌叶率和苗高生长等性状均存在极显著变异，试验数据表明种子体积北方红栎为大（其中宾夕法尼亚州家系１７２最大），其次是红栎和柳叶栎。千粒重以北方红栎为重（其中宾夕法尼亚州家系１７２最重），其次是红栎和柳叶栎。发芽率为北方红栎最高（其中宾夕法尼亚州家系１９１为１００％），其次是红栎和柳叶栎。３０ｄ萌叶率和苗高生长以柳叶栎为高（田纳西州种源９７０１ＴＮ最高），其次是红栎和北方红栎。在种间和种内不同种源、家系间种子千粒重与年终苗高生长相关不显著。

A high-performance liquid chromatography with circular dichroism detector for determination of stereochemistry of 6, 9-oxygen bridge dibenzocyclooctadiene lignans from kadsura coccinea

The stereochemistry of two 6, 9-oxygen bridge dibenzocyclooctadiene lignans from Kadsura coccinea, are difficult to separate and very unstable. The present study was designed to develop a high-performance liquid chromatography using circular dichroism detection for the analysis of the stereochemistry. A new 6, 9-oxygen bridge dibenzocyclooctadiene lignans named Kadsulignan Q was firstly found with an S-biphenyl configuration. The other compound was identified as Kadsulignan L with an R- biphenyl configuration. In order to obtain kinetic data on their reversible interconversion, the stability was measured at different deuterated solvents such as deuterated methanol, deuterated chloroform and deuterated dimethylsulfoxide. The lignans were more unstable and converted more easily in deuterated methanol than in deuterated chloroform and deuterated dimethylsulfoxide.