This study attempted to culture red sea bream lymphocytes using artificial medium supplemented with mitogens and a cytokine. Growth of the lymphocytes was compared between cultures with mitogens phytohemagglutinin (PH...This study attempted to culture red sea bream lymphocytes using artificial medium supplemented with mitogens and a cytokine. Growth of the lymphocytes was compared between cultures with mitogens phytohemagglutinin (PHA), lipopolysaccharide (LPS), concanavalin A(Con A) and a cytokine mouse interleukin 2(IL 2) in a basal medium at various combinations. Addition of PHA or LPS enhanced growth of the lymphocytes, Con A did not enhance growth so much. Large colonies were formed when PA was added. Con A induced scattered single cell proliferation of the lymphocytes. IL 2 supported the actions of PHA and LPS. Combined addition of PHA and IL 2 maintained proliferation for 30 d.展开更多
The effects of recombinant eel growth hormone (reGH).methyltestosterone (MT)and L-thyroxine (T4)on the growth of red sea bream. Pagrosomus major.were investigated.Administration of reGH to fry by immersion at 2 mg 1 f...The effects of recombinant eel growth hormone (reGH).methyltestosterone (MT)and L-thyroxine (T4)on the growth of red sea bream. Pagrosomus major.were investigated.Administration of reGH to fry by immersion at 2 mg 1 for 2 hevery 5 dsys resulted in significant increase in both weight and length.but the condition factor (CF) diminished relative to that of similarly treated controls over the 37day treatment period.Immersion in 0.1 mg:1 T4 also resulted in significant increase in both weight and length and higher survival rate of test fry compared to the controls. Immersion in MT had less effect on growth and high-dose resulted in high mortality.In the second study.injection of 2 μg reGH(gwk)caused a significant increase in the specific growth rate (SGR) of test red sea bream fingerlings relative to that of the controls during the 4-week treatment period and maintained the increasing trend over the post-treatment period (weeks 4-6).Injection of MT at a dosage of 1μg (gwk) resulted in a significant展开更多
Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH GS and rcsGH GS) or polymer matrix (reGH HP55) to protect the hormone fro...Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH GS and rcsGH GS) or polymer matrix (reGH HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH GS, rcsGH GS, reGH HP55 and free reGH or uncoated rcsGH were administered to red sea bream. Feeding of reGH GS, reGH HP55 and rcsGH GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.展开更多
Host-associated probiotics(HAPs)are bacteria originally isolated from rearing water or the host's gastrointestinal tract in order to enhance the host's growth and health.This study investigated the HAP potenti...Host-associated probiotics(HAPs)are bacteria originally isolated from rearing water or the host's gastrointestinal tract in order to enhance the host's growth and health.This study investigated the HAP potential of Bacillus sp.PM8313,isolated from wild red sea bream(Pagrus major),through characterization and feeding trials.Results based on in vitro tests showed that PM8313 is safe,confirming its hemolytic,cytotoxic,and antibiotic resistance.In addition,PM8313 showed advantages as a probiotic with high viability in the gastrointestinal model and a high cell adhesion rate.Whole-genome sequencing demonstrated that PM8313 has a 4,615,871 bp single circular chromosome and a guaninecytosine content of 45.25%.It also showed the absence of genes encoding virulence factors,such as cytotoxin,enterotoxin,hemolysin,sphingomyelinase,and phospholipase.In the feeding trial,a supplemental diet of 1×10^(8)CFU/g PM8313 positively altered the weight gain,digestive enzyme activity,and intestinal microbiota composition of red sea bream.Analysis of nonspecific immune parameters and immune-related gene expression,and a challenge test showed that PM8313 supplementation increases immunity and pathogenic bacteria resistance.Our findings suggest that PM8313 should be considered for application as a novel HAP to red sea bream aquaculture.展开更多
文摘This study attempted to culture red sea bream lymphocytes using artificial medium supplemented with mitogens and a cytokine. Growth of the lymphocytes was compared between cultures with mitogens phytohemagglutinin (PHA), lipopolysaccharide (LPS), concanavalin A(Con A) and a cytokine mouse interleukin 2(IL 2) in a basal medium at various combinations. Addition of PHA or LPS enhanced growth of the lymphocytes, Con A did not enhance growth so much. Large colonies were formed when PA was added. Con A induced scattered single cell proliferation of the lymphocytes. IL 2 supported the actions of PHA and LPS. Combined addition of PHA and IL 2 maintained proliferation for 30 d.
文摘The effects of recombinant eel growth hormone (reGH).methyltestosterone (MT)and L-thyroxine (T4)on the growth of red sea bream. Pagrosomus major.were investigated.Administration of reGH to fry by immersion at 2 mg 1 for 2 hevery 5 dsys resulted in significant increase in both weight and length.but the condition factor (CF) diminished relative to that of similarly treated controls over the 37day treatment period.Immersion in 0.1 mg:1 T4 also resulted in significant increase in both weight and length and higher survival rate of test fry compared to the controls. Immersion in MT had less effect on growth and high-dose resulted in high mortality.In the second study.injection of 2 μg reGH(gwk)caused a significant increase in the specific growth rate (SGR) of test red sea bream fingerlings relative to that of the controls during the 4-week treatment period and maintained the increasing trend over the post-treatment period (weeks 4-6).Injection of MT at a dosage of 1μg (gwk) resulted in a significant
文摘Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH GS and rcsGH GS) or polymer matrix (reGH HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH GS, rcsGH GS, reGH HP55 and free reGH or uncoated rcsGH were administered to red sea bream. Feeding of reGH GS, reGH HP55 and rcsGH GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.
基金financially supported by the grant (R2022016) from the National Institute of Fisheries Science,Republic of Koreathe National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2022R1A2C1012655).
文摘Host-associated probiotics(HAPs)are bacteria originally isolated from rearing water or the host's gastrointestinal tract in order to enhance the host's growth and health.This study investigated the HAP potential of Bacillus sp.PM8313,isolated from wild red sea bream(Pagrus major),through characterization and feeding trials.Results based on in vitro tests showed that PM8313 is safe,confirming its hemolytic,cytotoxic,and antibiotic resistance.In addition,PM8313 showed advantages as a probiotic with high viability in the gastrointestinal model and a high cell adhesion rate.Whole-genome sequencing demonstrated that PM8313 has a 4,615,871 bp single circular chromosome and a guaninecytosine content of 45.25%.It also showed the absence of genes encoding virulence factors,such as cytotoxin,enterotoxin,hemolysin,sphingomyelinase,and phospholipase.In the feeding trial,a supplemental diet of 1×10^(8)CFU/g PM8313 positively altered the weight gain,digestive enzyme activity,and intestinal microbiota composition of red sea bream.Analysis of nonspecific immune parameters and immune-related gene expression,and a challenge test showed that PM8313 supplementation increases immunity and pathogenic bacteria resistance.Our findings suggest that PM8313 should be considered for application as a novel HAP to red sea bream aquaculture.
