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Effects of Torreya grandis Root Rot on Seedling Growth and Screening of Its Chemical Control Agents
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作者 Lei ZHENG Xinyu RU +5 位作者 Ziyi WAN Chao HE Xiaoyu LI Caiyun LYU Li CHEN Zhibing WAN 《Agricultural Biotechnology》 CAS 2023年第5期108-113,共6页
[Objectives]This study was conducted to prevent the occurrence of root rot disease in Torreya grandis and improve the yield and quality of T.grandis.[Methods]One-year-old and two-year-old seedlings of Torreya grandis... [Objectives]This study was conducted to prevent the occurrence of root rot disease in Torreya grandis and improve the yield and quality of T.grandis.[Methods]One-year-old and two-year-old seedlings of Torreya grandis‘Xifei’and‘Cufei’were inoculated with the root rot pathogen Fusarium fujikuroi,and the changes in photosynthesis,chlorophyll content,malondialdehyde,and defense enzyme system(superoxide dismutase,peroxidase,and catalase)activity in leaves of T.grandis seedlings were investigated using water as a control.Meanwhile,the control effects of 80%carbendazim wettable powder,64%metalaxyl·mancozeb wettable powder,430 g/L tebuconazole suspension and 30%difenoconazole·cyproconazole EC on root rot in T.grandis were investigated.[Results]After inoculation with the pathogen F.fujikuroi,the net photosynthetic rates and transpiration rates in leaves of T.grandis‘Xifei’and‘Cufei’decreased,and the contents of chlorophyll decreased,while the contents of malondialdehyde increased,and the contents of superoxide dismutase and catalase increased with time.However,peroxidase showed a high activity in T.grandis‘Cufei’only,but a trend of"increasing-decreasing-increasing"in T.grandis‘Xifei’.Among the four chemical control agents,64%metalaxyl·mancozeb wettable powder had the best control effect on root rot of T.grandis caused by the pathogenic fungus F.fujikuroi.[Conclusions]The net photosynthetic rate,transpiration rate,chlorophyll content,malondialdehyde content,superoxide dismutase activity and catalase activity could all be used as screening indicators for T.grandis varieties resistant to root rot.Meanwhile,64%metalaxyl·mancozeb wettable powder could be used as a control agent for root rot on T.grandis. 展开更多
关键词 Torreya grandis root rot disease Physiological and biochemical metabolism Chemical control
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Formulation of Biofungicides from Cymbopogon citratus and Tithonia diversifolia: Evaluating Its Antimicrobial Activities against Pythium myriotylum, the Causal Agent of Root Rot of Xanthosoma sagittifolium (L.) Schott
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作者 Magni Pacha Tatiana Flore Tene Tayo Paul Martial +4 位作者 Foka Tatiekam Ebenezer Dzelamonyuy Aristide Ewane Cecile Annie Mbouopda Herman Désire Boudjeko Thaddée 《American Journal of Plant Sciences》 2023年第8期896-914,共7页
Three fungicide formulations, namely M1, M2 and M3, were prepared from sodium bicarbonate, citronella essential oil and sunflower slurry. The stability of M1, M2 and M3 formulations was determined based on pH, tempera... Three fungicide formulations, namely M1, M2 and M3, were prepared from sodium bicarbonate, citronella essential oil and sunflower slurry. The stability of M1, M2 and M3 formulations was determined based on pH, temperature, order of incorporation of the inputs and storage time. The most stable formulations were used for antagonistic tests on Pythium myriotylum. The Minimum Inhibitory Concentration (MIC) was used for the greenhouse tests and the mode of action was determined in vitro. The study showed that the order of incorporation of the inputs “Essential Oil-Tween 80-Bicarbonate-Slurry” (EO-T80-B-S) promotes stability. M1 and M2 are stable at 4°C, 25°C, 37°C and 40°C temperatures and have a pH of 7 and 8 respectively. The Minimum Inhibitory Concentration of M1 and M2 is 1% on P. myriotylum. M1 and M2 act on Pythium by membrane lysis, inhibiting proton pumps and inhibiting protein synthesis. The formulations M1 and M2 reduce the incidence of root rot disease in cocoyam plants growth in the greenhouse. M1 and M2 are potential candidates for improvement of cocoyam seedlings production in Cameroon. 展开更多
关键词 BIOFUNGICIDE Xanthosoma sagittifolium Pythium myriotylum root Rot Disease
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Prevention and Control of Gentiana macrophylla Pall Root Rot Disease
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作者 程远辉 侯志江 徐中志 《Agricultural Science & Technology》 CAS 2014年第11期2039-2040,共2页
The root rot is a major disease affecting yield of Gentiana macrophyl a pal , reducing yield or even causing total crop failure. This research introduced the occurrence characteristics and various control techniques o... The root rot is a major disease affecting yield of Gentiana macrophyl a pal , reducing yield or even causing total crop failure. This research introduced the occurrence characteristics and various control techniques of root rot of Gentiana macrophyl a pal , in order to provide references for farmers. 展开更多
关键词 Gentiana macrophyl a pal root rot disease Prevention and control
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A LAMP-assay-based specific microbiota analysis reveals community dynamics and potential interactions of 13 major soybean root pathogens 被引量:6
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作者 YE Wen-wu ZENG Dan-dan +4 位作者 XU Miao YANG Jin MA Jia-xin WANG Yuan-chao ZHENG Xiao-bo 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第8期2056-2063,共8页
Soybean root diseases are associated with numerous fungal and oomycete pathogens;however,the community dynamics and interactions of these pathogens are largely unknown.We performed 13 loop-mediated isothermal amplific... Soybean root diseases are associated with numerous fungal and oomycete pathogens;however,the community dynamics and interactions of these pathogens are largely unknown.We performed 13 loop-mediated isothermal amplification(LAMP)assays that targeted specific soybean root pathogens,and traditional isolation assays.A total of 159 samples were collected from three locations in the Huang-Huai-Hai region of China at three soybean growth stages(30,60,and 90 days after planting)in 2016.In LAMP results,we found that pathogen communities differed slightly among locations,but changed dramatically between soybean growth stages.Phytophthora sojae,Rhizoctonia solani,and Fusarium oxysporum were most frequently detected at the early stage,whereas Phomopsis longicolla,Fusarium equiseti,and Fusarium virguliforme were most common in the later stages.Most samples(86%)contained two to six pathogen species.Interestingly,the less detectable species tended to exist in the samples containing more detected species,and some pathogens preferentially co-occurred in diseased tissue,including P.sojae–R.solani–F.oxysporum and F.virguliforme–Calonectria ilicicola,implying potential interactions during infection.The LAMP detection results were confirmed by traditional isolation methods.The isolated strains exhibited different virulence to soybean,further implying a beneficial interaction among some pathogens. 展开更多
关键词 soybean root disease fungal and oomycete plant pathogens soil-borne and seed-borne pathogens LAMP assay complex infection
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First Report of Fusarium striatum Causing Root Rot Disease of Panax notoginseng in Yunnan,China
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作者 Xuan Zhou Chongyu Luo +6 位作者 Kuixiu Li Dan Zhu Lihui Jiang Lixia Wu Youchun Li Xiahong He Yunlong Du 《Phyton-International Journal of Experimental Botany》 SCIE 2022年第1期13-20,共8页
Panax notoginseng is a traditional Chinese medicinal plant.Root rot of P.notoginseng is one of the most serious diseases affecting P.notoginseng growth and causes wilted leaves,fewer lateral roots and rotten roots.Roo... Panax notoginseng is a traditional Chinese medicinal plant.Root rot of P.notoginseng is one of the most serious diseases affecting P.notoginseng growth and causes wilted leaves,fewer lateral roots and rotten roots.Root rot is a soil-borne disease,and mainly occurs from June to August in Yunnan Province when the temperatures are high and the air is humid.In this study,the endophytic fungal genus Fusarium isolate E-2018.1.22-#3.2 was obtained from a P.notoginseng embryo.Fusarium isolate E-2018.1.22-#3.2 was identified as Fusarium striatum based on morphological characteristics and molecular analysis.The fungus was found to have conidiophores and macroconidia,and its ITS,LSU and TEF-1αgenes shared 100%,99.2%and 99%identities with the homologous genes of Fusarium striatum,respectively.Isolate F.striatum E-2018.1.22-#3.2 can cause root rot symptoms,including black,soft roots,fewer lateral roots and leaf wilt,in 93%of the experimental P.notoginseng plants,and could be re-isolated,fulfilling Koch’s postulates.When the P.notoginseng plants were treated with the fungicide pyraclostrobin,isolate F.striatum E-2018.1.22-#3.2 was unable to cause root rot.We have therefore demonstrated that F.striatum E-2018.1.22-#3.2 is able to cause root rot disease in P.notoginseng.This is the first report of root rot disease caused by F.striatum on P.notoginseng in China. 展开更多
关键词 Panax notoginseng Fusarium striatum root rot disease endophytic fungus
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Characterization and functional analysis of gerbera plant defensin(PDF)genes reveal the role of GhPDF2.4 in defense against the root rot pathogen Phytophthora cryptogea
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作者 Chunzhen Cheng Huan Wu Yongyan Zhang 《aBIOTECH》 EI CAS CSCD 2024年第3期325-338,共14页
Gerbera(Gerbera hybrida),a major fresh cut flower crop,is very susceptible to root rot disease.Although plant defensins(PDFs),a major group of plant antimicrobial peptides,display broad-spectrum antifungal and antibac... Gerbera(Gerbera hybrida),a major fresh cut flower crop,is very susceptible to root rot disease.Although plant defensins(PDFs),a major group of plant antimicrobial peptides,display broad-spectrum antifungal and antibacterial activities,PDF genes in gerbera have not been systematically characterized.Here,we identified and cloned nine PDF genes from gerbera and divided them into two classes based on phylogenetic analysis.Most Class Ⅰ GhPDF genes were highly expressed in petioles,whereas all Class Ⅱ GhPDF genes were highly expressed in roots.Phytophthora cryptogea inoculation strongly upregulated all Class II GhPDF genes in roots and upregulated all Class Ⅰ GhPDF genes in petioles.Transient overexpression of GhPDF1.5 and GhPDF2.4 inhibited P.cryptogea infection in tobacco(Nicotiana benthamiana)leaves.Transient overexpression of GhPDF2.4,but not GhPDF1.5,significantly upregulated ACO and LOX gene expression in tobacco leaves,indicating that overexpressing GhPDF2.4 activated the jasmonic acid/ethylene defense pathway and that the two types of GhPDFs have different modes of action.Prokaryotically expressed recombinant GhPDF2.4 inhibited mycelial growth and delayed the hyphal swelling of P.cryptogea,in vitro,indicating that GhPDF2.4 is a morphogenetic defensin.Moreover,the addition of GhPDF2.4 to plant culture medium alleviated the root rot symptoms of in vitro-grown gerbera seedlings and greatly reduced pathogen titer in P.cryptogea-inoculated gerbera roots in the early stages of treatment.Our study provides a basis for the use of GhPDFs,especially GhPDF2.4,for controlling root rot disease in gerbera. 展开更多
关键词 Plant defensin root rot disease Antifungal activity
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Acupuncture Principle of Tonifying Qi and Regulating Blood,Supporting the Root and Fostering the Source on Aging and Senile Diseases 被引量:7
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作者 韩景献 《Chinese Journal of Integrative Medicine》 SCIE CAS 2007年第3期166-167,共2页
Along with the increasing life span, aging and related diseases have become a serious medical and social problem that has roused global attention. In this paper, under the guidance of traditional Chinese medicine (TC... Along with the increasing life span, aging and related diseases have become a serious medical and social problem that has roused global attention. In this paper, under the guidance of traditional Chinese medicine (TCM), the author raises a theory of "dysfunction of Sanjiao qi activity" based on the studies and discussions of classical literatures on Sanjiao theory by combining knowledge in modern integrative traditional Chinese and Western medicine for aging from his more than 30 years of experiences of clinical and experimental practices. The author also tries to explain the mechanisms for aging from the whole aspect of Sanjiao qi activity. 展开更多
关键词 SOURCE Acupuncture Principle of Tonifying Qi and Regulating Blood Supporting the root and Fostering the Source on Aging and Senile diseases
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Vitapex糊剂用于根尖诱导成形术的疗效观察 被引量:6
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作者 赵芬 林志勇 季梅 《山东大学学报(医学版)》 CAS 北大核心 2009年第3期63-65,70,共4页
目的观察Vitapex糊剂用于根尖诱导成形术的临床疗效。方法应用Vitapex糊剂对62例患牙进行根尖诱导,详细记录治疗过程,观察治疗效果,分析不同牙根发育状况、根尖周骨质破坏状况及不同年龄组对疗效的影响。结果根尖诱导成形术的总有效率为... 目的观察Vitapex糊剂用于根尖诱导成形术的临床疗效。方法应用Vitapex糊剂对62例患牙进行根尖诱导,详细记录治疗过程,观察治疗效果,分析不同牙根发育状况、根尖周骨质破坏状况及不同年龄组对疗效的影响。结果根尖诱导成形术的总有效率为95.2%,牙根不同的发育状况、根尖周骨质破坏的不同程度及不同年龄组的临床疗效之间均无统计学差异(P>0.05)。结论Vitapex糊剂在各种患牙的根尖诱导成形术中均疗效显著。 展开更多
关键词 根尖周疾病 根管充填材料 氢氧化钙
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