Study on the Cutting Rooting of Syriga Microphylla with Tender Branches

[Objective] The study was carried out in order to find out the best method of rapid cutting rooting of Syriga microphylla with tender branches.[Method] Effect of rooting reagent with different concentrations on the rapid cutting rooting of Syriga microphylla with tender branches was studied in our paper.[Result] There was great difference between cuttings with auxin and without auxin.[Conclusion] Tender branches treated by 90 mg/L indole butyric acid（IBA）had the best cutting rooting.

Effects of Root Growth of Deep and Shallow Rooting Rice Cultivars in Compacted Paddy Soils on Subsequent Rice Growth

Rice is often grown as multiple seasons in one year,alternating between flooded and upland systems.A major constraint,introduced from the flooded system,is a plough pan that may decrease rooting depth and productivity of follow-on upland rice.Roots penetrating the plough pan under flooded rice system can leave a legacy of weaker root growth pathways.Deeper rooting rice cultivars could have a bigger impact,but no direct evidence is available.To explore whether a deep rather than a shallow rooting rice cultivar grown in a flooded cropping cycle benefited deeper root growth of follow-on rice in an upland,reduced tillage cropping cycle,a simulated flooded paddy in greenhouse was planted with deep(Black Gora) and shallow(IR64) rooting cultivars and a plant-free control.Artificial plough pans were made in between the topsoil and subsoil to form different treatments with no plough pan(0.35 MPa),soft plough pan(1.03 MPa) and hard plough pan(1.70 MPa).After harvest of this ‘first season’ rice,the soil was drained and undisturbed to simulate zero-tillage upland and planted rice cultivar BRRI Dhan 28.The overall root length density(RLD),root surface area,the numbers of root tips and branching of BRRI Dhan 28 did not vary between plough pan and no plough pan treatments.Compared with the shallow rooting rice genotype,the deep rooting rice genotype as ‘first season’ crop produced 19% greater RLD,34% greater surface area and 29% more branching of BRRI Dhan 28 in the subsoil.In the topsoil,however,BRRI Dhan 28 had 28% greater RLD,35% greater surface area and 43% more branching for the shallow rather than deep rooting genotype planted in the ‘first season’.The results suggested that rice cultivar selection for a paddy cycle affects root growth of a follow-on rice crop grown under no-till,with benefits to subsoil access from deep rooting cultivars and topsoil proliferation for shallow rooting cultivars.

Establishment of Somatic Cell Clones in Thesium chinense Turcz and Its in vitro Rooting Technique

[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz.[Method]With stem fragments of wild Thesium chinense Turcz as explants,different culture media were designed to conduct induction culture,strengthening plantlet culture and in vitro rooting.[Result]The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA,0.01 mg/L NAA and 0.3 mg/L 2,4-D;in addition,60 mg/kg ABT was suitable for rooting,by which the percentage of rooted plantlets reached 76.6%.[Conclusion]This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate,providing basis for artificial cultivation and resource protection of Thesium chinense Turcz.

基于DWT的Alpha-Rooting图像增强

针对数字图像的频域增强,提出一种基于离散小波变换的Alpha-Rooting图像对比度增强方法.该方法利用小波的多分辨率性质,结合Alpha-Rooting图像对比度增强特点,对高频细节信号采用Alpha-Rooting算法增强,低频近似信号采用归一化增强算法.实验结果表明,该方法在EME与直方图两个尺度上,优于其他Alpha-Rooting增强算法(DCT、FFT)的对比度增强效果.

Effects of Different Exogenous Hormones on Rooting of Syringa microphylla Cuttings and Change in Related Enzyme Activity During the Rooting Process

[Objective] This study aimed to investigate the effects of different exoge- nous hormones on the rooting of Syringa microphylla cuttings and the change in related enzymes activity during the rooting process. [Method] Three different exoge- nous hormones IBA, NAA and ABT, each with concentrations of 500, 1 000, 1 500 and 2 000 mg/L were used to treat S. microphylla cuttings, and changes in the ac- tivities of peroxidase （POD）, poiyphenol oxidase （PPO） and indoleacetic acid oxidase （IAAO） during the rooting process were also investigated. [Result] The most appro- priate concentrations of IBA, ABT and NAA were 1 500, 1 000 and 1 000 mg/L, respectively, and the 1 500 mg/L IBA treatment exhibited the best effect on rooting. Throughout the rooting process, POD and PPO activities showed the same trends in the treatment groups as those in the control group, but the POD and PPO activi- ties in the treatment groups were increased significantly, with greater amplitude of variation; at the early stage, IAAO activity exhibited an opposite trend between the control group and the treatment groups, which increased slowly in the former, but decreased rapidly in the latter, and it was significantly lower in the treatment groups compared to the control; additionally, higher POD and IAAO activities were con- ducive to the induction of adventitious roots, and lower POD and IAAO activities fa- vored their formation and elongation. [Conclusion] This study has preliminarily clari- fied the rooting mechanism of S. microphylla cuttings.

Effects of Remaining Leaf Combining with IBA on Rooting,Physiological and Biochemical Indicators of Leaves from Bougainvillea spectabilis Cuttings

[Objective] The research aimed to discuss the effects on rooting,physiological and biochemical indicators of Bougainvillea spectabilis cuttings by remaining leaf combining with soaking cuttings in IBA.[Method] Effects of remaining leaf combining with IBA on rooting,physiological and biochemical indicators of B.spectabilis cuttings were studied under the regulation and control of intelligent seed propagation system,and then set four treatments：remaining leaf combining with soaking in clean water,remaining leaf combining with soaking in IBA,defoliation combining soaking in clean water,and defoliation combining with soaking in IBA.[Result] The soluble sugar content was high,but indoleacetic acid oxidase activity was lower in the initial stage of adventitious root differentiation in treatment of remaining leaf.While the rooting number,root length,the root mass growth and survival rate of cutting were obviously higher than that in treatment of defoliation.Compared to the treatment soaking in clean water,the treatment soaking in 500×10^-6 IBA increased the soluble sugar content in leaves of cuttings and enhanced rooting of cuttings.[Conclusion] The survival rate and rooting of cuttings improved obviously in the treatment of remaining leaf combining with soaking in 500×10^-6 IBA before cutting under the regulation and control of intelligent seed propagation system.

Effect of Different Factors on Rooting Characteristics and Survival of Lonicera japonica Thunb

[Objective] The aim was to explore the effects of different factors on rapid propagation and seedling survival of Lonicera japonica Thunb. [Method] The cuttings of 1, 2 or 3 years old, with different length, were soaked in different hormones at different concentrations for different time and planted at different dates. Finally, their rooting rate and survival rate in different treatments were measured and compared. [Result] The rooting rate and survival rate of one-year old cuttings with length of 10-13 cm and planted on September 20 and October 20, 2012 were relatively higher among all the treatments. Hormone treatment could significantly promote the callus formation and accelerate the rooting of the cuttings, and improve their survival rate. The cuttings treated with 120-200 mg/L ABT1 or NAA for 35-45 min had higher rooting rate and survival rate. Among them, the survival rate of cuttings treated with 160 mg/L ABT1 for 35 min was the highest, up to 92.5%. [Conclusion] The study provided basis for improving the survival rate of L. japonica by cuttage, and for optimizing the rapid propagation technique.

Effect of Water Control before Transplanting and Rooting Powder Treatment on Tobacco Seedling Quality and Physiological Properties at Green Stage

Cultivating strong seedlings is an important guarantee for the production of high-quality flue-cured tobacco, while there are many disadvantages in tobacco floating system that is commonly adopted in China. To improve the tobacco floating system, with Xiangyan No.3 as experimental material, the effects of water control before transplanting and rooting powder treatment on tobacco seedling quality and physiological properties at green stage were investigated. The results showed that： （1） water control showed small influence on tobacco seedling quality, while rooting powder treatment and water control ＋ rooting powder treatment showed great influence on tobacco seedling quality, mainly represented by reduced plant height, thickened stem and increased dry matter accumulative amount; （2） water control before transplanting and rooting powder treatment all improved leaf chlorophyll content and root vigor of tobacco seedlings, and the effect of water control ＋ rooting powder treatment was best, followed by rooting powder treatment and water control; （3） all treatments increased the nitrate reductase and invertase activity, and reduced the MDA content of tobacco seedlings, and the effect of water control ＋ rooting powder treatment was best, followed by rooting powder treatment and water control. Mean- while, the treatment effect 10 d before the transplanting was better than that 5 d before the transplanting. In overall, the improvement effects of water control 10 d before transplanting ＋ rooting powder treatment on tobacco seedling quality and physiological properties at green stage were the best.

Study on Multiplication, Rooting and Transplanting of Tissue Culture Plantlets of Rhododendron chrysanthum Pall

[Objective] This study aimed to shorten the multiplication culture and root- ing culture periods of Rh. chrysanthum Pall. [Method] The Rh. chrysanthum Pall tis- sue culture plantlets collected from Changbai Mountain were used as material, and the effects of different hormone combinations and coconut milk on the proliferation and differentiation of Rh. chrysanthum Pall tissue culture plantlets were investigated. In addition, the rooting medium and transplanting matrix for Rh. chrysanthum Pall tissue culture plantlets were explored. [Result] The medium composed of modified MS, iBA （3 mg/L） and ZT （1.5 mg/L） was the optimum medium for subculture mul- tiplication of Rh. chrysanthum Pall tissue culture plantlets. The multiplication multiple and average plant height were significantly improved by adding coconut milk into the medium （150 mg/L）. [Conclusion] For Rh. chrysanthum Pall tissue culture plantlets, the optimum rooting culture medium was composed of modified MS （1/4） and IBA （5.0 mg/L）, and the tissue culture plantlets began to root 8 d after the inoculation. The root induction treatment was carried out after a 15-d sand culture, and the suitable matrix was composed of tufty soil, humus soil and perlite （2：1：1） with a survival rate of 95.66%.

Research on Plantlet Rooting of Lagerstroemia indica

Tissue culture plantlets of Lagerstroemia indica were used to study the effects of different auxins on in-vitro rooting, and the effects of various media in transplanting of rooted plantlets and ex-vitro rooting. For in-vitro rooting of test-tube plantiets of Lagerstroemia indica, 0.5 mg/L IBA is a suitable growth regulator; in the case of transplanting of rooted plantlets, the medium ratio of peat soil to perlite at 7：3 is better; and as to ex-vitro rooting of Lagerstroemia indica plantlets, the medium ratio of peat soil to perlite at 3：7 is more suitable.

Polynomial rooting based frequency offset estimation for MIMO OFDM systems

Based on the frequency domain training sequences, the polynomial-based carrier frequency offset （CFO） estimation in multiple-input multiple-output （ MIMO ） orthogonal frequency division multiplexing （ OFDM ） systems is extensively investigated. By designing the training sequences to meet certain conditions and exploiting the Hermitian and real symmetric properties of the corresponding matrices, it is found that the roots of the polynomials corresponding to the cost functions are pairwise and that both meger CFO and fractional CFO can be estimated by the direct polynomial rooting approach. By analyzing the polynomials corresponding to the cost functions and their derivatives, it is shown that they have a common polynomial factor and the former can be expressed in a quadratic form of the common polynomial factor. Analytical results further reveal that the derivative polynomial rooting approach is equivalent to the direct one in estimation at the same signal-to-noise ratio（SNR） value and that the latter is superior to the former in complexity. Simulation results agree well with analytical results.

Research on Rooting Cuttings of Hard Branch of Hippophae rhamnoides

Objective] In order to improve the survival rate of Hippophae rhamnoides hardwood cuttings, cultivate high-quality sea buckthorn seedlings. [Method] This pa-per took hard branches of Russian big fruit H. rhamnoides as material. The days of striking roots, rate of striking roots,root length and number of adventitious root were determined. [Result] The cuttings col ected from upper treetops were obviously inferi-or to the lower ones. Three years cutting is obviously better than one or two years. Cuttings from lower branches were not so good as the cuttings from upper branch-es. Base oblique incisions were clearly superior to paper-cover incision. The optimal length of cuttings was 20-25 cm. The rooting rate of cuttings was the highest by fast dipping with NAA of 50 mg/L. [Conclusion] The study provides theoretical basis for H. rhamnoides artificial cultivation.

Technology Research of Plantlets Rooting and Transplanting on Tissue Culture of Ilex centrochinensis

Plantlets of Ilex centrochinensis were used in its tissue culture to study the effect of auxin and gibberellin in vitro rooting, and the effect of various matrix in transplanting rooted plantlets and in rooting outside the tube. The highest fre- quency of rooting was obtained when growth regulator combination was IBA 0.2 mg/L ＋ GA3 0.4 mg/L in vitro rooting, and when the ratio of sand and humus soil was 5 to 5 in rooting outside the tube. When the ratio of perlite and peat soil was 3 to 7, the highest survival of rooted plantlets in transplanting was obtained. This study may provide the basis for improving the factory system of tissue culture of llex centrochinensis.

Effects of mother tree ages, different rooting mediums, light conditions and auxin treatments on rooting behaviour of Dalbergia sissoo branch cuttings

Dalbergia sissoo Roxb. is one of the promising multipurpose tree species of South Asia. Most of the plantations of D. sissoo from seeds are facing severe threats due to the die-back disease, which ultimately causes death of this potential tree-species within a few months. Vegetative propagation could avoid the die-back disease. Thirty mother trees of different age-groups of D. sissoo were selected for evaluating the rooting behaviour of branch cuttings from D. sissoo as influenced by auxins （IAA or IBA at 100, 200, 500 mg·L^-1）, ages of mother trees （10, 4 and 2 years old） and different environment conditions, i.e., different mediums （soil and sand） or light conditions （in shade and open condition）. The results show that application of IAA and IBA induced more numbers of cuttings （collected from 10-year-old mother trees） to root compared to control. Branch cuttings of D. sissoo collected from 10-year-old mother trees and planted in soil bed in open conditions had 100.0% of cuttings to root in IAA （100 mg·L^-1） and IBA （200 mg·L^-1） treatments. Both rooting medium （Soil and sand） influenced significantly （p〈0.05） on rooting response of branch cuttings. Soil medium was found to achieve maximum no. of branch cuttings to root, compared to sand medium.

Effects of IBA on the rooting of branch cuttings of Chinese jujube (Zizyphus jujuba Mill.) and changes to nutrients and endogenous hormones

‘Zhongqiusucui’ jujube secondary shoots were treated with 3-indolebutyric acid(IBA) at three concentrations, 500, 1000 and 1500 mg/L. Results show that IBA could significantly enhance rooting and root characteristics of cuttings and were best with IBA at 1500 mg/L. In the rooting process, the formation of adventitious roots was related to the consumption and accumulation of nutrients(soluble sugars and proteins) and the changes in endogenous hormones in phloem, leaf tips and leaf bases. The rooting of cuttings had a positive correlation with the consumption of soluble sugars during the period of callus formation and with the accumulation of soluble sugars during adventitious root formation and growth. Rooting was positively related to the breakdown of soluble proteins in the phloem when the callus formed, and had a positive correlation with its accumulation during adventitious root formation and growth. Leaf tips and leaf bases showed a reverse trend in changes of soluble protein. However, together with the phloem, leaf tips and leaf bases regulated and controlled the formation and development of adventitious roots. The main activities of soluble proteins exist in the leaf tips as this was the main source of soluble proteins. The relation between rooting and IAA(indole-3-acetic acid) content in phloem was positive and thus a high concentration of IAA could benefit the induction and formation of adventitious roots. However, rooting was negatively related with ABA(abscisic acid) and GA(gibberellic acid) and a high concentration of both could inhibit the induction and formation of adventitious roots. Rooting had a positive correlation with phloem IAA/ABA ratios, and higher ratios could improve rooting. Low concentrations of ZR(zeatin riboside) triggered the induction of adventitious roots, while higher concentrations promoted root growth.Endogenous hormones in leaf tips and bases had an impact on rooting. The activities of endogenous hormones mainly existed in leaf tips because they play a major role in the production and consumption of IAA and its ABA content increased during rooting. The ZR in leaf tips influenced the rooting of cuttings, especially in the callus formation and rooting stage. Leaf tips were the main source of GA.

The type, position and age effect on the cutting reproduction of Picea crassifolia and its rooting mechanism in the Qilian Mountains

Picea crassifolia Kom, a perennial arbor spe- cies is recognized as one of the most adaptable plants found to date in Qilian Mountains. To explore the cutting reproduction technology of P. crassifolia and reveal its rooting mechanism, cuttings of P. crassifolia with different cutting types （softwood, hardwood and root）, positions （top, upper, middle and bottom） and ages （7, 10, 15, 20, 25 year-old） were cultivated in a field experiment. One- year old softwood and hardwood cuttings were collected from 7-, 10-, 15-, 20-, and 25 year-old healthy ortets to analyze the changes from endogenous hormones and organic nutrients. Results indicate that the softwood cut- tings （0.5-1.0 cm in diameter） from upper branches of 15 year-old ortets shows better growth performance by improving rooting indexes, including a significant increasein rooting rate and a decrease in basal rot rate. Concomi- tantly, increasing rooting quantity and root length also increased. It is noteworthy that the high rooting rate of P. crassifolia cuttings due to its ability to accumulate high concentrations of indole-3-acetic acid （IAA） and total carbon （TC） rather than abscisic acid （ABA） and total nitrogen （TN）. The rooting rate was mainly regulated by the IAA/ABA and TC/TN ratio. In summary, our results suggest that the softwood cuttings （0.5-1.0 cm in diameter） from upper branches of 15 year-old P. crassifolia can be considered as an effective strategy to improve cutting rooting rate, and the IAA/ABA and TC/TN ratio was one of the main factors limiting the cutting rooting rate of P. crassifolia.

Effects of Lanthanum and Europium on Rooting of Plantlet Eriobotrya Japonica Lindl. in vitro

The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the length of root were studied after transplanting 20 d. The activity of peroxidase, nitrate reductase and fresh weight of roots were determined after transplanting 44 d. The results show that the optimum concentration range of La 3+ (1.0～3.0 μmol·L -1), Eu 3+ (2.0～3.0 μmol·L -1) in the rooted medium can increase the rooting rate and the fresh weight of roots, and promote the length of root and raise the activities of peroxidase and nitrate reductase significantly. La 3+ has more effect in improving the rooting rate, root length and the activities of peroxidase and less effect in promoting root fresh weight and the activities of nitrate reductase than Eu 3+.

Changes in endogenous hormone levels and redox status during enhanced adventitious rooting by rare earth element neodymium of Dendrobium densiflorum shoot cuttings

The effects of neodymium nitrate （Nd^3＋） on the adventitious rooting of Dendrobium densiflorum shoot cuttings were studied. The addition of Nd^3＋ （5 μmol/L） to culture medium significantly increased rooting frequency. Histological investigation showed that Nd^3＋ did not change the process of root initiation. Nd^3＋ did not influence total endogenous cytokinin levels, but significantly increased the level of endogenous indole-3-acetic acid （IAA） in the base of shoot cuttings. Compared to the control, the ratio of IAA/cytokinins was very high in the Nd^3＋ treatment. These results suggested that the enhanced rooting frequency may be related to the increase in endogenous IAA level in Nd^3＋ treatment. Analysis of enzyme activities showed that the enhanced accumulation of the endogenous IAA by Nd^3＋ should not be attributed to inhibition of IAA decomposition by IAA oxidase or promotion of cytokinin decomposition by cytokinin oxidase. Besides, Nd^3＋ increased the ratio of reduced glutathione （GSH） and oxidized glutathione （GSSG） in the process of adventitious rooting while the ratio of ascorbate （ASC） to dehydroascorbate （DHA） was not affected.

Effect of rooting media and hormone concentrations on vegetative propagation of Balanites aegyptiaca

Vegetative propagation is widely used as a means of genotype preservation in clone banks and seed orchards but despite this, the possibility of vegetative propagation using hormones is not clear for many indigenous tree species, including Balanites aegyptiaca. This research is on the vegetative propagation of B. aegyptiaca as influenced by rooting media and growth hormones.Juvenile stem cuttings were obtained from 4 months old seedlings. Nodal stem cuttings 4–5 cm long from 3–5 mm diameter stems were treated with indole-3-butyric acid or indole-3-acetic acid at 50, 100 and 150 mg/L and sown in three different rooting media: top soil, river sand, and sawdust. The results reveal a significant effect(p \ 0.05)of rooting media and growth hormones on B. aegyptiaca cuttings. B. aegyptiaca can be propagated vegitatively without the use of hormones. The control was recommended for vegetative propagation of B. aegyptiaca.

Optimization of Rooting Medium for Tissue Culture Seedlings of Dendrobium officinale by Response Surface Methodology

[Objectives] To increase the survival rate of tissue culture seedlings of Dendrobium officinale,and optimize the conditions of rooting medium by the response surface methodology( RSM). [Methods]The effects of 6-BA concentration,NAA concentration,potato amount and the amount of mashed banana on the growth of seedlings were determined by single factor experiment and were analyzed by Box-Behnken design and response surface methodology. [Results]The optimal culture conditions: rooting medium 1/2 MS + 6-BA 0. 24 mg/L + mashed banana 87. 63 g/L + potato 89. 30 g/L + NAA 0. 52 mg/L + sucrose 20. 0 g/L + activated carbon 4. 0 g/L + agar 7. 0 g/L,p H 5. 8,and light intensity 2 000 Lx. [Conclusions]The model established by response surface methodology has a good predictability and could be used to optimize the conditions of tissue culture and rooting medium of D. officinale.